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Protein

Thiosulfate sulfurtransferase PspE

Gene

pspE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspE catalyzes the sulfur-transfer reaction from thiosulfate to cyanide, to form sulfite and thiocyanate. Also able to use dithiol (dithiothreitol) as an alternate sulfur acceptor. Also possesses a very low mercaptopyruvate sulfurtransferase activity.

Miscellaneous

Catalysis proceeds by a classical ping-pong bi-bi reaction mechanism, whereby a covalent enzyme-sulfur intermediate is formed on the active site cysteine.

Catalytic activityi

Thiosulfate + cyanide = sulfite + thiocyanate.1 Publication

Enzyme regulationi

Inhibited by thiosulfate above 100 mM, particularly at low cyanide concentrations (<5 mM). Inhibited by sodium sulfate or sodium chloride at 0.25 M which gives around 50% inhibition of rhodanese activity. Addition of sodium phosphate at the same concentration results in about 65% inhibition. Sulfite strongly inhibits PspE activity (1 mM sodium sulfite resulted in more than 50% inhibition of rhodanese activity).2 Publications

Kineticsi

  1. KM=2.7 mM for thiosulfate (at pH 8.6 and at 25 degrees Celsius)2 Publications
  2. KM=10 mM for dithiothreitol (at pH 8.6 and at 25 degrees Celsius)2 Publications
  3. KM=32 mM for cyanide (at pH 8.6 and at 25 degrees Celsius)2 Publications
  1. Vmax=410 µmol/min/mg enzyme toward cyanide (at pH 8.6 and at 25 degrees Celsius)2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67Cysteine persulfide intermediate1
Sitei93May be important for providing the necessary conformational flexibility for enzymatic catalysis1

GO - Molecular functioni

  • thiosulfate sulfurtransferase activity Source: EcoCyc

Keywordsi

Molecular functionTransferase
Biological processStress response

Enzyme and pathway databases

BioCyciEcoCyc:EG10780-MONOMER.
MetaCyc:EG10780-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiosulfate sulfurtransferase PspE (EC:2.8.1.1)
Short name:
TST
Alternative name(s):
Phage shock protein E
Gene namesi
Name:pspE
Ordered Locus Names:b1308, JW1301
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10780. pspE.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
  • periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000003043120 – 104Thiosulfate sulfurtransferase PspEAdd BLAST85

Proteomic databases

PaxDbiP23857.
PRIDEiP23857.

Expressioni

Inductioni

By heat, ethanol, osmotic shock and infection by filamentous bacteriophages. Expression is positively regulated by cyclic AMP-cAMP receptor protein (cAMP-CRP). Expressed at higher levels during growth on glycerol, acetate or proline as carbon source relative to expression during growth on glucose.3 Publications

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4263234. 400 interactors.
DIPiDIP-10591N.
IntActiP23857. 3 interactors.
STRINGi316385.ECDH10B_1425.

Structurei

Secondary structure

1104
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 25Combined sources5
Helixi29 – 32Combined sources4
Beta strandi35 – 37Combined sources3
Beta strandi40 – 42Combined sources3
Helixi45 – 55Combined sources11
Beta strandi61 – 70Combined sources10
Helixi71 – 82Combined sources12
Beta strandi86 – 93Combined sources8
Turni94 – 96Combined sources3
Beta strandi101 – 103Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JTQNMR-A20-104[»]
2JTRNMR-A20-104[»]
2JTSNMR-A20-104[»]
ProteinModelPortaliP23857.
SMRiP23857.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23857.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 104RhodanesePROSITE-ProRule annotationAdd BLAST85

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105K8Z. Bacteria.
COG0607. LUCA.
HOGENOMiHOG000247776.
InParanoidiP23857.
KOiK03972.
PhylomeDBiP23857.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiView protein in InterPro
IPR014323. Phageshock_PspE.
IPR001763. Rhodanese-like_dom.
PfamiView protein in Pfam
PF00581. Rhodanese. 1 hit.
SMARTiView protein in SMART
SM00450. RHOD. 1 hit.
SUPFAMiSSF52821. SSF52821. 1 hit.
TIGRFAMsiTIGR02981. phageshock_pspE. 1 hit.
PROSITEiView protein in PROSITE
PS50206. RHODANESE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23857-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKKGLLALA LVFSLPVFAA EHWIDVRVPE QYQQEHVQGA INIPLKEVKE
60 70 80 90 100
RIATAVPDKN DTVKVYCNAG RQSGQAKEIL SEMGYTHVEN AGGLKDIAMP

KVKG
Length:104
Mass (Da):11,475
Last modified:November 1, 1991 - v1
Checksum:i52D52FB8FC0CC943
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57560 Genomic DNA. Translation: CAA40793.1.
U00096 Genomic DNA. Translation: AAC74390.1.
AP009048 Genomic DNA. Translation: BAA14877.1.
PIRiS17125.
RefSeqiNP_415824.1. NC_000913.3.
WP_000473109.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74390; AAC74390; b1308.
BAA14877; BAA14877; BAA14877.
GeneIDi945652.
KEGGiecj:JW1301.
eco:b1308.
PATRICifig|1411691.4.peg.971.

Similar proteinsi

Entry informationi

Entry nameiPSPE_ECOLI
AccessioniPrimary (citable) accession number: P23857
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: September 27, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references