Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Trk system potassium uptake protein TrkG

Gene

trkG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Low-affinity potassium transport system. Interacts with Trk system potassium uptake protein TrkA and requires TrkE for transport activity. Can transport potassium and rubidium.3 Publications

Kineticsi

  1. KM=1 mM for K+1 Publication
  2. KM=0.4 mM for Rb+1 Publication
  1. Vmax=300 µmol/min/g enzyme with K+ as substrate1 Publication
  2. Vmax=17 µmol/min/g enzyme with Rb+ as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei114 – 1141Potassium ion; via carbonyl oxygenBy similarity
Binding sitei115 – 1151Potassium ion; via carbonyl oxygenBy similarity
Binding sitei224 – 2241Potassium ion; via carbonyl oxygenBy similarity
Binding sitei437 – 4371Potassium ion; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciEcoCyc:TRKG-MONOMER.
ECOL316407:JW1358-MONOMER.
MetaCyc:TRKG-MONOMER.

Protein family/group databases

TCDBi2.A.38.1.6. the k(+) transporter (trk) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Trk system potassium uptake protein TrkG
Gene namesi
Name:trkG
Ordered Locus Names:b1363, JW1358
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11020. trkG.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini? – 485CytoplasmicBy similarity
Topological domaini? – 391CytoplasmicBy similarity
Intramembranei? – 302By similarity
Topological domaini? – 276CytoplasmicBy similarity
Intramembranei? – 100By similarity
Topological domaini1 – 55CytoplasmicBy similarity
Transmembranei6 – 3227HelicalBy similarityAdd
BLAST
Topological domaini33 – 386PeriplasmicBy similarity
Transmembranei39 – 6022HelicalBy similarityAdd
BLAST
Topological domaini61 – 688CytoplasmicBy similarity
Transmembranei69 – 9325HelicalBy similarityAdd
BLAST
Topological domaini94 – ?PeriplasmicBy similarity
Intramembranei101 – 11212Helical; Pore-formingBy similarityAdd
BLAST
Intramembranei113 – 1186By similarity
Topological domaini119 – 1279PeriplasmicBy similarity
Transmembranei128 – 15326HelicalBy similarityAdd
BLAST
Topological domaini154 – 18027CytoplasmicBy similarityAdd
BLAST
Transmembranei181 – 20525HelicalBy similarityAdd
BLAST
Topological domaini206 – 2083PeriplasmicBy similarity
Intramembranei209 – 2091By similarity
Intramembranei210 – 22112Helical; Pore-formingBy similarityAdd
BLAST
Intramembranei222 – 2276By similarity
Topological domaini228 – 23710PeriplasmicBy similarity
Intramembranei238 – 25316HelicalBy similarityAdd
BLAST
Intramembranei254 – ?By similarity
Transmembranei277 – 29721HelicalBy similarityAdd
BLAST
Topological domaini298 – ?PeriplasmicBy similarity
Intramembranei303 – 31816Helical; Pore-formingBy similarityAdd
BLAST
Intramembranei319 – 3246By similarity
Topological domaini325 – 3328PeriplasmicBy similarity
Intramembranei333 – 34412HelicalBy similarityAdd
BLAST
Intramembranei345 – 35713Note=Loop between two helicesBy similarityAdd
BLAST
Intramembranei358 – ?HelicalBy similarity
Transmembranei392 – 41928HelicalBy similarityAdd
BLAST
Topological domaini420 – 4212PeriplasmicBy similarity
Intramembranei422 – 4232By similarity
Intramembranei424 – 43411Helical; Pore-formingBy similarityAdd
BLAST
Intramembranei435 – 4417By similarity
Topological domaini442 – 45312PeriplasmicBy similarityAdd
BLAST
Intramembranei454 – 46512HelicalBy similarityAdd
BLAST
Intramembranei466 – ?By similarity

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Trk system potassium uptake protein TrkGPRO_0000070474Add
BLAST

Proteomic databases

PaxDbiP23849.

Interactioni

Protein-protein interaction databases

BioGridi4260161. 11 interactions.
DIPiDIP-11029N.
STRINGi511145.b1363.

Structurei

3D structure databases

ProteinModelPortaliP23849.
SMRiP23849. Positions 7-484.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni113 – 1186Selectivity filter part 1By similarity
Regioni222 – 2276Selectivity filter part 2By similarity
Regioni319 – 3246Selectivity filter part 3By similarity
Regioni436 – 4416Selectivity filter part 4By similarity

Sequence similaritiesi

Belongs to the TrkH potassium transport family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D37. Bacteria.
COG0168. LUCA.
HOGENOMiHOG000225541.
InParanoidiP23849.
KOiK03498.
OMAiTINFATH.
OrthoDBiEOG63589N.
PhylomeDBiP23849.

Family and domain databases

InterProiIPR003445. Cat_transpt.
IPR004772. TrkH.
[Graphical view]
PfamiPF02386. TrkH. 1 hit.
[Graphical view]
PIRSFiPIRSF006247. TrkH. 1 hit.
TIGRFAMsiTIGR00933. 2a38. 1 hit.

Sequencei

Sequence statusi: Complete.

P23849-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTSHVRVVT HMCGFLVWLY SLSMLPPMVV ALFYKEKSLF VFFITFVIFF
60 70 80 90 100
CIGGGAWYTT KKSGIQLRTR DGFIIIVMFW ILFSVISAFP LWIDSELNLT
110 120 130 140 150
FIDALFEGVS GITTTGATVI DDVSSLPRAY LYYRSQLNFI GGLGVIVLAV
160 170 180 190 200
AVLPLLGIGG AKLYQSEMPG PFKDDKLTPR LADTSRTLWI TYSLLGIACI
210 220 230 240 250
VCYRLAGMPL FDAICHGIST VSLGGFSTHS ESIGYFNNYL VELVAGSFSL
260 270 280 290 300
LSAFNFTLWY IVISRKTIKP LIRDIELRFF LLIALGVIIV TSFQVWHIGM
310 320 330 340 350
YDLHGSFIHS FFLASSMLTD NGLATQDYAS WPTHTIVFLL LSSFFGGCIG
360 370 380 390 400
STCGGIKSLR FLILFKQSKH EINQLSHPRA LLSVNVGGKI VTDRVMRSVW
410 420 430 440 450
SFFFLYTLFT VFFILVLNGM GYDFLTSFAT VAACINNMGL GFGATASSFG
460 470 480
VLNDIAKCLM CIAMILGRLE IYPVIILFSG FFWRS
Length:485
Mass (Da):53,944
Last modified:November 1, 1991 - v1
Checksum:i87E19BEC3B50B458
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56783 Genomic DNA. Translation: CAA40103.1.
U00096 Genomic DNA. Translation: AAC74445.1.
AP009048 Genomic DNA. Translation: BAA14960.1.
PIRiA39408.
RefSeqiNP_415881.1. NC_000913.3.
WP_001097895.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC74445; AAC74445; b1363.
BAA14960; BAA14960; BAA14960.
GeneIDi945932.
KEGGiecj:JW1358.
eco:b1363.
PATRICi32118004. VBIEscCol129921_1422.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56783 Genomic DNA. Translation: CAA40103.1.
U00096 Genomic DNA. Translation: AAC74445.1.
AP009048 Genomic DNA. Translation: BAA14960.1.
PIRiA39408.
RefSeqiNP_415881.1. NC_000913.3.
WP_001097895.1. NZ_CP014272.1.

3D structure databases

ProteinModelPortaliP23849.
SMRiP23849. Positions 7-484.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260161. 11 interactions.
DIPiDIP-11029N.
STRINGi511145.b1363.

Protein family/group databases

TCDBi2.A.38.1.6. the k(+) transporter (trk) family.

Proteomic databases

PaxDbiP23849.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74445; AAC74445; b1363.
BAA14960; BAA14960; BAA14960.
GeneIDi945932.
KEGGiecj:JW1358.
eco:b1363.
PATRICi32118004. VBIEscCol129921_1422.

Organism-specific databases

EchoBASEiEB1013.
EcoGeneiEG11020. trkG.

Phylogenomic databases

eggNOGiENOG4105D37. Bacteria.
COG0168. LUCA.
HOGENOMiHOG000225541.
InParanoidiP23849.
KOiK03498.
OMAiTINFATH.
OrthoDBiEOG63589N.
PhylomeDBiP23849.

Enzyme and pathway databases

BioCyciEcoCyc:TRKG-MONOMER.
ECOL316407:JW1358-MONOMER.
MetaCyc:TRKG-MONOMER.

Miscellaneous databases

PROiP23849.

Family and domain databases

InterProiIPR003445. Cat_transpt.
IPR004772. TrkH.
[Graphical view]
PfamiPF02386. TrkH. 1 hit.
[Graphical view]
PIRSFiPIRSF006247. TrkH. 1 hit.
TIGRFAMsiTIGR00933. 2a38. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Subcloning, nucleotide sequence, and expression of trkG, a gene that encodes an integral membrane protein involved in potassium uptake via the Trk system of Escherichia coli."
    Schloesser A., Kluttig S., Hamann A., Bakker E.P.
    J. Bacteriol. 173:3170-3176(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "K+-transport protein TrkA of Escherichia coli is a peripheral membrane protein that requires other trk gene products for attachment to the cytoplasmic membrane."
    Bossemeyer D., Borchard A., Dosch D.C., Helmer G.C., Epstein W., Booth I.R., Bakker E.P.
    J. Biol. Chem. 264:16403-16410(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "TrkH and its homolog, TrkG, determine the specificity and kinetics of cation transport by the Trk system of Escherichia coli."
    Schlosser A., Meldorf M., Stumpe S., Bakker E.P., Epstein W.
    J. Bacteriol. 177:1908-1910(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiTRKG_ECOLI
AccessioniPrimary (citable) accession number: P23849
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 11, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.