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Protein

Trk system potassium uptake protein TrkG

Gene

trkG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Low-affinity potassium transport system. Interacts with Trk system potassium uptake protein TrkA and requires TrkE for transport activity. Can transport potassium and rubidium.3 Publications

Kineticsi

  1. KM=1 mM for K+1 Publication
  2. KM=0.4 mM for Rb+1 Publication
  1. Vmax=300 µmol/min/g enzyme with K+ as substrate1 Publication
  2. Vmax=17 µmol/min/g enzyme with Rb+ as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114Potassium ion; via carbonyl oxygenBy similarity1
Binding sitei115Potassium ion; via carbonyl oxygenBy similarity1
Binding sitei224Potassium ion; via carbonyl oxygenBy similarity1
Binding sitei437Potassium ion; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciEcoCyc:TRKG-MONOMER.
ECOL316407:JW1358-MONOMER.
MetaCyc:TRKG-MONOMER.

Protein family/group databases

TCDBi2.A.38.1.6. the k(+) transporter (trk) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Trk system potassium uptake protein TrkG
Gene namesi
Name:trkG
Ordered Locus Names:b1363, JW1358
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11020. trkG.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini? – 485CytoplasmicBy similarity
Topological domaini? – 391CytoplasmicBy similarity
Intramembranei? – 302By similarity
Topological domaini? – 276CytoplasmicBy similarity
Intramembranei? – 100By similarity
Topological domaini1 – 5CytoplasmicBy similarity5
Transmembranei6 – 32HelicalBy similarityAdd BLAST27
Topological domaini33 – 38PeriplasmicBy similarity6
Transmembranei39 – 60HelicalBy similarityAdd BLAST22
Topological domaini61 – 68CytoplasmicBy similarity8
Transmembranei69 – 93HelicalBy similarityAdd BLAST25
Topological domaini94 – ?PeriplasmicBy similarity
Intramembranei101 – 112Helical; Pore-formingBy similarityAdd BLAST12
Intramembranei113 – 118By similarity6
Topological domaini119 – 127PeriplasmicBy similarity9
Transmembranei128 – 153HelicalBy similarityAdd BLAST26
Topological domaini154 – 180CytoplasmicBy similarityAdd BLAST27
Transmembranei181 – 205HelicalBy similarityAdd BLAST25
Topological domaini206 – 208PeriplasmicBy similarity3
Intramembranei209By similarity1
Intramembranei210 – 221Helical; Pore-formingBy similarityAdd BLAST12
Intramembranei222 – 227By similarity6
Topological domaini228 – 237PeriplasmicBy similarity10
Intramembranei238 – 253HelicalBy similarityAdd BLAST16
Intramembranei254 – ?By similarity
Transmembranei277 – 297HelicalBy similarityAdd BLAST21
Topological domaini298 – ?PeriplasmicBy similarity
Intramembranei303 – 318Helical; Pore-formingBy similarityAdd BLAST16
Intramembranei319 – 324By similarity6
Topological domaini325 – 332PeriplasmicBy similarity8
Intramembranei333 – 344HelicalBy similarityAdd BLAST12
Intramembranei345 – 357Note=Loop between two helicesBy similarityAdd BLAST13
Intramembranei358 – ?HelicalBy similarity
Transmembranei392 – 419HelicalBy similarityAdd BLAST28
Topological domaini420 – 421PeriplasmicBy similarity2
Intramembranei422 – 423By similarity2
Intramembranei424 – 434Helical; Pore-formingBy similarityAdd BLAST11
Intramembranei435 – 441By similarity7
Topological domaini442 – 453PeriplasmicBy similarityAdd BLAST12
Intramembranei454 – 465HelicalBy similarityAdd BLAST12
Intramembranei466 – ?By similarity

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000704741 – 485Trk system potassium uptake protein TrkGAdd BLAST485

Proteomic databases

PaxDbiP23849.
PRIDEiP23849.

Interactioni

Protein-protein interaction databases

BioGridi4260161. 11 interactors.
DIPiDIP-11029N.
STRINGi511145.b1363.

Structurei

3D structure databases

ProteinModelPortaliP23849.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni113 – 118Selectivity filter part 1By similarity6
Regioni222 – 227Selectivity filter part 2By similarity6
Regioni319 – 324Selectivity filter part 3By similarity6
Regioni436 – 441Selectivity filter part 4By similarity6

Sequence similaritiesi

Belongs to the TrkH potassium transport family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D37. Bacteria.
COG0168. LUCA.
HOGENOMiHOG000225541.
InParanoidiP23849.
KOiK03498.
OMAiISMTIMI.
PhylomeDBiP23849.

Family and domain databases

InterProiIPR003445. Cat_transpt.
IPR004772. TrkH.
[Graphical view]
PfamiPF02386. TrkH. 1 hit.
[Graphical view]
PIRSFiPIRSF006247. TrkH. 1 hit.
TIGRFAMsiTIGR00933. 2a38. 1 hit.

Sequencei

Sequence statusi: Complete.

P23849-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTSHVRVVT HMCGFLVWLY SLSMLPPMVV ALFYKEKSLF VFFITFVIFF
60 70 80 90 100
CIGGGAWYTT KKSGIQLRTR DGFIIIVMFW ILFSVISAFP LWIDSELNLT
110 120 130 140 150
FIDALFEGVS GITTTGATVI DDVSSLPRAY LYYRSQLNFI GGLGVIVLAV
160 170 180 190 200
AVLPLLGIGG AKLYQSEMPG PFKDDKLTPR LADTSRTLWI TYSLLGIACI
210 220 230 240 250
VCYRLAGMPL FDAICHGIST VSLGGFSTHS ESIGYFNNYL VELVAGSFSL
260 270 280 290 300
LSAFNFTLWY IVISRKTIKP LIRDIELRFF LLIALGVIIV TSFQVWHIGM
310 320 330 340 350
YDLHGSFIHS FFLASSMLTD NGLATQDYAS WPTHTIVFLL LSSFFGGCIG
360 370 380 390 400
STCGGIKSLR FLILFKQSKH EINQLSHPRA LLSVNVGGKI VTDRVMRSVW
410 420 430 440 450
SFFFLYTLFT VFFILVLNGM GYDFLTSFAT VAACINNMGL GFGATASSFG
460 470 480
VLNDIAKCLM CIAMILGRLE IYPVIILFSG FFWRS
Length:485
Mass (Da):53,944
Last modified:November 1, 1991 - v1
Checksum:i87E19BEC3B50B458
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56783 Genomic DNA. Translation: CAA40103.1.
U00096 Genomic DNA. Translation: AAC74445.1.
AP009048 Genomic DNA. Translation: BAA14960.1.
PIRiA39408.
RefSeqiNP_415881.1. NC_000913.3.
WP_001097895.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC74445; AAC74445; b1363.
BAA14960; BAA14960; BAA14960.
GeneIDi945932.
KEGGiecj:JW1358.
eco:b1363.
PATRICi32118004. VBIEscCol129921_1422.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56783 Genomic DNA. Translation: CAA40103.1.
U00096 Genomic DNA. Translation: AAC74445.1.
AP009048 Genomic DNA. Translation: BAA14960.1.
PIRiA39408.
RefSeqiNP_415881.1. NC_000913.3.
WP_001097895.1. NZ_CP014272.1.

3D structure databases

ProteinModelPortaliP23849.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260161. 11 interactors.
DIPiDIP-11029N.
STRINGi511145.b1363.

Protein family/group databases

TCDBi2.A.38.1.6. the k(+) transporter (trk) family.

Proteomic databases

PaxDbiP23849.
PRIDEiP23849.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74445; AAC74445; b1363.
BAA14960; BAA14960; BAA14960.
GeneIDi945932.
KEGGiecj:JW1358.
eco:b1363.
PATRICi32118004. VBIEscCol129921_1422.

Organism-specific databases

EchoBASEiEB1013.
EcoGeneiEG11020. trkG.

Phylogenomic databases

eggNOGiENOG4105D37. Bacteria.
COG0168. LUCA.
HOGENOMiHOG000225541.
InParanoidiP23849.
KOiK03498.
OMAiISMTIMI.
PhylomeDBiP23849.

Enzyme and pathway databases

BioCyciEcoCyc:TRKG-MONOMER.
ECOL316407:JW1358-MONOMER.
MetaCyc:TRKG-MONOMER.

Miscellaneous databases

PROiP23849.

Family and domain databases

InterProiIPR003445. Cat_transpt.
IPR004772. TrkH.
[Graphical view]
PfamiPF02386. TrkH. 1 hit.
[Graphical view]
PIRSFiPIRSF006247. TrkH. 1 hit.
TIGRFAMsiTIGR00933. 2a38. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRKG_ECOLI
AccessioniPrimary (citable) accession number: P23849
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.