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Protein

Middle operon regulator

Gene

mor

Organism
Enterobacteria phage Mu (Bacteriophage Mu)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Activator of the Pm promoter, which controls middle genes expression. Activation of Pm allows expression of protein C necessary for late gene expression. In addition to Mor binding, activation of Pm might require the interaction of Mor with the C-terminus of host RNAP subunits RpoA and RpoH.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi94 – 11421H-T-H motifSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Middle operon regulator
Short name:
Mor
Alternative name(s):
GemB
Gene product 17
Short name:
gp17
Gene namesi
Name:mor
Ordered Locus Names:Mup17
OrganismiEnterobacteria phage Mu (Bacteriophage Mu)
Taxonomic identifieri10677 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMulikevirus
Virus hostiEnterobacteriaceae [TaxID: 543]
Proteomesi
  • UP000002611 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi65 – 651G → M, N, R, T or V: Complete loss of DNA-binding and transcription activation ability. 1 Publication
Mutagenesisi66 – 661G → A, H, L, P or R: Complete loss of DNA-binding and transcription activation ability. 1 Publication
Mutagenesisi68 – 681Q → G, F, I, L, K, P or R: Complete loss of DNA-binding and transcription activation ability. 1 Publication
Mutagenesisi70 – 701Y → G, I, R, S or V: Complete loss of DNA-binding and transcription activation ability. 1 Publication
Mutagenesisi74 – 741G → F or W: Partial loss of DNA-binding and transcription activation ability. 1 Publication
Mutagenesisi74 – 741G → I, S, T or Y: Partial loss of DNA-binding and transcription activation ability. 1 Publication
Mutagenesisi74 – 741G → R: Complete loss of DNA-binding and transcription activation ability. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 129129Middle operon regulatorPRO_0000062803Add
BLAST

Expressioni

Inductioni

This protein is constitively expressed from the Pgem promoter unlike most other early proteins which are expressed under the control of the Pe promoter. Its expression seems to escape repression by repressor protein c and thus could occur throughout latency (Probable).1 Publication

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.2 Publications

Structurei

Secondary structure

1
129
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi28 – 4518Combined sources
Helixi53 – 6412Combined sources
Helixi75 – 8915Combined sources
Helixi95 – 1028Combined sources
Helixi106 – 11813Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RR7X-ray2.20A1-129[»]
ProteinModelPortaliP23848.
SMRiP23848. Positions 27-120.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23848.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni28 – 6437DimerizationAdd
BLAST
Regioni77 – 12953DNA-bindingAdd
BLAST

Domaini

The dimerization domain in the N-terminus and the DNA-binding domain in the C-terminus are connected by a linker containing a beta-strand.

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR009057. Homeodomain-like.
IPR014875. Mor_transcription_activator.
[Graphical view]
PfamiPF08765. Mor. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.

Sequencei

Sequence statusi: Complete.

P23848-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEDLFGDLQ DDTILAHLDN PAEDTSRFPA LLAELNDLLR GELSRLGVDP
60 70 80 90 100
AHSLEIVVAI CKHLGGGQVY IPRGQALDSL IRDLRIWNDF NGRNVSELTT
110 120
RYGVTFNTVY KAIRRMRRLK YRQYQPSLL
Length:129
Mass (Da):14,714
Last modified:November 1, 1991 - v1
Checksum:iC796C950F1686FBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64097 Genomic DNA. Translation: AAA32408.1.
AF083977 Genomic DNA. Translation: AAF01094.1.
RefSeqiNP_050621.1. NC_000929.1.

Genome annotation databases

GeneIDi2636272.
KEGGivg:2636272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64097 Genomic DNA. Translation: AAA32408.1.
AF083977 Genomic DNA. Translation: AAF01094.1.
RefSeqiNP_050621.1. NC_000929.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RR7X-ray2.20A1-129[»]
ProteinModelPortaliP23848.
SMRiP23848. Positions 27-120.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2636272.
KEGGivg:2636272.

Miscellaneous databases

EvolutionaryTraceiP23848.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR009057. Homeodomain-like.
IPR014875. Mor_transcription_activator.
[Graphical view]
PfamiPF08765. Mor. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a positive regulator of the Mu middle operon."
    Mathee K., Howe M.M.
    J. Bacteriol. 172:6641-6650(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence of the left end of Mu."
    Priess H., Brauer B., Schmidt C., Kamp D.
    (In) Symonds N., Toussaint A., van de Putte P., Howe M.M. (eds.); Phage Mu, pp.277-296, Cold Spring Harbor Laboratory Press, New York (1987)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus."
    Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.
    J. Mol. Biol. 317:337-359(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The bacteriophage Mu gem gene: a positive regulator of the C operon required for normal levels of late transcription."
    Giusti M., Di Lallo G., Ghelardini P., Paolozzi L.
    Virology 179:694-700(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The Mu gem operon: its role in gene expression, recombination and cell cycle."
    Ghelardini P., La Valle R., Paolozzi L.
    Genetica 94:151-156(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  6. "Distortion in the spacer region of Pm during activation of middle transcription of phage Mu."
    Artsimovitch I., Kahmeyer-Gabbe M., Howe M.M.
    Proc. Natl. Acad. Sci. U.S.A. 93:9408-9413(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Regulation of the bacteriophage Mu gem operon."
    Fabozzi G., Paolozzi L., Ghelardini P.
    Virology 241:73-79(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "Genetic analysis of phage Mu Mor protein amino acids involved in DNA minor groove binding and conformational changes."
    Kumaraswami M., Avanigadda L., Rai R., Park H.W., Howe M.M.
    J. Biol. Chem. 286:35852-35862(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING, SUBUNIT, DIMERIZATION DOMAIN, MUTAGENESIS OF GLY-65; GLY-66; GLN-68; TYR-70 AND GLY-74.
  9. "Crystal structure of the Mor protein of bacteriophage Mu, a member of the Mor/C family of transcription activators."
    Kumaraswami M., Howe M.M., Park H.W.
    J. Biol. Chem. 279:16581-16590(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), DNA-BINDING, SUBUNIT.

Entry informationi

Entry nameiMOR_BPMU
AccessioniPrimary (citable) accession number: P23848
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: December 9, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.