P23845 (CYSN_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate adenylyltransferase subunit 1
EC=2.7.7.4

Alternative name(s):
ATP-sulfurylase large subunit
Sulfate adenylate transferase
Short name=SAT

Gene names

Name:	cysN
Ordered Locus Names:	b2751, JW2721

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be the GTPase, regulating ATP sulfurylase activity. HAMAP-Rule MF_00062
Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP-Rule MF_00062
Enzyme regulation	GTPase activity is coupled to stimulation of the rate of APS formation. HAMAP-Rule MF_00062
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP-Rule MF_00062
Subunit structure	Heterodimer composed of CysD, the smaller subunit, and CysN.
Sequence similarities	Belongs to the GTP-binding elongation factor family. CysN/NodQ subfamily.

Ontologies

Keywords
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	hydrogen sulfide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway sulfate assimilation Inferred from electronic annotation. Source: HAMAP sulfur compound metabolic process Inferred from mutant phenotype Ref.4. Source: EcoliWiki
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from sequence or structural similarity PubMed 1730615. Source: EcoliWiki GTPase activity Inferred from direct assay PubMed 1730615. Source: EcoliWiki sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Sulfate adenylyltransferase subunit 1 HAMAP-Rule MF_00062		PRO_0000091522
Regions
	Nucleotide binding	34 – 41	8	GTP By similarity
	Nucleotide binding	113 – 117	5	GTP By similarity
	Nucleotide binding	168 – 171	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23845 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 91E983E49732AA15	FASTA	475	52,558
	10 20 30 40 50 60 MNTALAQQIA NEGGVEAWMI AQQHKSLLRF LTCGSVDDGK STLIGRLLHD TRQIYEDQLS 70 80 90 100 110 120 SLHNDSKRHG TQGEKLDLAL LVDGLQAERE QGITIDVAYR YFSTEKRKFI IADTPGHEQY 130 140 150 160 170 180 TRNMATGAST CELAILLIDA RKGVLDQTRR HSFISTLLGI KHLVVAINKM DLVDYSEETF 190 200 210 220 230 240 TRIREDYLTF AGQLPGNLDI RFVPLSALEG DNVASQSESM PWYSGPTLLE VLETVEIQRV 250 260 270 280 290 300 VDAQPMRFPV QYVNRPNLDF RGYAGTLASG RVEVGQRVKV LPSGVESNVA RIVTFDGDRE 310 320 330 340 350 360 EAFAGEAITL VLTDEIDISR GDLLLAADEA LPAVQSASVD VVWMAEQPLS PGQSYDIKIA 370 380 390 400 410 420 GKKTRARVDG IRYQVDINNL TQREVENLPL NGIGLVDLTF DEPLVLDRYQ QNPVTGGLIF 430 440 450 460 470 IDRLSNVTVG AGMVHEPVSQ ATAAPSEFSA FELELNALVR RHFPHWGARD LLGDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence of the sulfate activation locus from Escherichia coli K-12." Leyh T.S., Vogt T.F., Suo Y. J. Biol. Chem. 267:10405-10410(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The sulfate activation locus of Escherichia coli K12: cloning, genetic, and enzymatic characterization." Leyh T.S., Taylor J.C., Markham G.D. J. Biol. Chem. 263:2409-2416(1988) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M74586 Genomic DNA. Translation: AAA23646.1. U29579 Genomic DNA. Translation: AAA69261.1. U00096 Genomic DNA. Translation: AAC75793.1. AP009048 Genomic DNA. Translation: BAE76828.1.
PIR	JN0327.
RefSeq	NP_417231.1. NC_000913.2. YP_490960.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P23845.
SMR	P23845. Positions 22-434.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-513N.
IntAct	P23845. 2 interactions.
MINT	MINT-1247685.
STRING	511145.b2751.
Proteomic databases
PaxDb	P23845.
PRIDE	P23845.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75793; AAC75793; b2751. BAE76828; BAE76828; BAE76828.
GeneID	12933938. 947219.
KEGG	ecj:Y75_p2689. eco:b2751.
PATRIC	32120908. VBIEscCol129921_2846.
Organism-specific databases
EchoBASE	EB0191.
EcoGene	EG10194. cysN.
Phylogenomic databases
eggNOG	COG2895.
HOGENOM	HOG000229289.
KO	K00956.
OMA	HDSAQIY.
ProtClustDB	PRK05124.
Enzyme and pathway databases
BioCyc	EcoCyc:CYSN-MONOMER. ECOL316407:JW2721-MONOMER. MetaCyc:CYSN-MONOMER.
SABIO-RK	P23845.
UniPathway	UPA00140; UER00204.
Gene expression databases
Genevestigator	P23845.
Family and domain databases
HAMAP	MF_00062. Sulf_adenylyltr_sub1.
InterPro	IPR000795. EF_GTP-bd_dom. IPR005225. Small_GTP-bd_dom. IPR011779. SO4_adenylTrfase_lsu. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR02034. CysN. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSN_ECOLI

Accession

Primary (citable) accession number: P23845
Secondary accession number(s): Q2MA78

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	May 1, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program