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Protein

Sulfate adenylyltransferase subunit 1

Gene

cysN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be the GTPase, regulating ATP sulfurylase activity.

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.

Enzyme regulationi

GTPase activity is coupled to stimulation of the rate of APS formation.

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 41GTPBy similarity8
Nucleotide bindingi113 – 117GTPBy similarity5
Nucleotide bindingi168 – 171GTPBy similarity4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • GTPase activity Source: EcoliWiki
  • GTP binding Source: EcoliWiki
  • sulfate adenylyltransferase (ATP) activity Source: EcoCyc

GO - Biological processi

  • hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
  • sulfur compound metabolic process Source: EcoliWiki

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:CYSN-MONOMER
MetaCyc:CYSN-MONOMER
SABIO-RKP23845
UniPathwayiUPA00140; UER00204

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 1 (EC:2.7.7.4)
Alternative name(s):
ATP-sulfurylase large subunit
Sulfate adenylate transferase
Short name:
SAT
Gene namesi
Name:cysN
Ordered Locus Names:b2751, JW2721
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10194 cysN

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000915221 – 475Sulfate adenylyltransferase subunit 1Add BLAST475

Proteomic databases

PaxDbiP23845
PRIDEiP23845

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.

Protein-protein interaction databases

BioGridi4262932, 28 interactors
DIPiDIP-513N
IntActiP23845, 3 interactors
STRINGi316385.ECDH10B_2919

Structurei

3D structure databases

ProteinModelPortaliP23845
SMRiP23845
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 239tr-type GAdd BLAST215

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 41G1By similarity8
Regioni92 – 96G2By similarity5
Regioni113 – 116G3By similarity4
Regioni168 – 171G4By similarity4
Regioni206 – 208G5By similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C3T Bacteria
COG2895 LUCA
HOGENOMiHOG000229289
InParanoidiP23845
KOiK00956
OMAiSRGDMIC
PhylomeDBiP23845

Family and domain databases

HAMAPiMF_00062 Sulf_adenylyltr_sub1, 1 hit
InterProiView protein in InterPro
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR011779 SO4_adenylTrfase_lsu
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02034 CysN, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P23845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTALAQQIA NEGGVEAWMI AQQHKSLLRF LTCGSVDDGK STLIGRLLHD
60 70 80 90 100
TRQIYEDQLS SLHNDSKRHG TQGEKLDLAL LVDGLQAERE QGITIDVAYR
110 120 130 140 150
YFSTEKRKFI IADTPGHEQY TRNMATGAST CELAILLIDA RKGVLDQTRR
160 170 180 190 200
HSFISTLLGI KHLVVAINKM DLVDYSEETF TRIREDYLTF AGQLPGNLDI
210 220 230 240 250
RFVPLSALEG DNVASQSESM PWYSGPTLLE VLETVEIQRV VDAQPMRFPV
260 270 280 290 300
QYVNRPNLDF RGYAGTLASG RVEVGQRVKV LPSGVESNVA RIVTFDGDRE
310 320 330 340 350
EAFAGEAITL VLTDEIDISR GDLLLAADEA LPAVQSASVD VVWMAEQPLS
360 370 380 390 400
PGQSYDIKIA GKKTRARVDG IRYQVDINNL TQREVENLPL NGIGLVDLTF
410 420 430 440 450
DEPLVLDRYQ QNPVTGGLIF IDRLSNVTVG AGMVHEPVSQ ATAAPSEFSA
460 470
FELELNALVR RHFPHWGARD LLGDK
Length:475
Mass (Da):52,558
Last modified:November 1, 1991 - v1
Checksum:i91E983E49732AA15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74586 Genomic DNA Translation: AAA23646.1
U29579 Genomic DNA Translation: AAA69261.1
U00096 Genomic DNA Translation: AAC75793.1
AP009048 Genomic DNA Translation: BAE76828.1
PIRiJN0327
RefSeqiNP_417231.1, NC_000913.3
WP_001090361.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75793; AAC75793; b2751
BAE76828; BAE76828; BAE76828
GeneIDi947219
KEGGiecj:JW2721
eco:b2751
PATRICifig|1411691.4.peg.3989

Similar proteinsi

Entry informationi

Entry nameiCYSN_ECOLI
AccessioniPrimary (citable) accession number: P23845
Secondary accession number(s): Q2MA78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: March 28, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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