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Protein

Sulfate adenylyltransferase subunit 1

Gene

cysN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be the GTPase, regulating ATP sulfurylase activity.

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.

Enzyme regulationi

GTPase activity is coupled to stimulation of the rate of APS formation.

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi34 – 418GTPBy similarity
Nucleotide bindingi113 – 1175GTPBy similarity
Nucleotide bindingi168 – 1714GTPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • GTPase activity Source: EcoliWiki
  • GTP binding Source: EcoliWiki
  • sulfate adenylyltransferase (ATP) activity Source: EcoCyc

GO - Biological processi

  • hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
  • sulfate assimilation Source: UniProtKB-HAMAP
  • sulfur compound metabolic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:CYSN-MONOMER.
ECOL316407:JW2721-MONOMER.
MetaCyc:CYSN-MONOMER.
SABIO-RKP23845.
UniPathwayiUPA00140; UER00204.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 1 (EC:2.7.7.4)
Alternative name(s):
ATP-sulfurylase large subunit
Sulfate adenylate transferase
Short name:
SAT
Gene namesi
Name:cysN
Ordered Locus Names:b2751, JW2721
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10194. cysN.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Sulfate adenylyltransferase subunit 1PRO_0000091522Add
BLAST

Proteomic databases

PaxDbiP23845.
PRIDEiP23845.

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.

Protein-protein interaction databases

BioGridi4262932. 18 interactions.
DIPiDIP-513N.
IntActiP23845. 3 interactions.
MINTiMINT-1247685.
STRINGi511145.b2751.

Structurei

3D structure databases

ProteinModelPortaliP23845.
SMRiP23845. Positions 22-434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 239215tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 418G1By similarity
Regioni92 – 965G2By similarity
Regioni113 – 1164G3By similarity
Regioni168 – 1714G4By similarity
Regioni206 – 2083G5By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG2895. LUCA.
HOGENOMiHOG000229289.
InParanoidiP23845.
KOiK00956.
OMAiRGNMLVS.
OrthoDBiEOG6GR38Q.
PhylomeDBiP23845.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00062. Sulf_adenylyltr_sub1.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTALAQQIA NEGGVEAWMI AQQHKSLLRF LTCGSVDDGK STLIGRLLHD
60 70 80 90 100
TRQIYEDQLS SLHNDSKRHG TQGEKLDLAL LVDGLQAERE QGITIDVAYR
110 120 130 140 150
YFSTEKRKFI IADTPGHEQY TRNMATGAST CELAILLIDA RKGVLDQTRR
160 170 180 190 200
HSFISTLLGI KHLVVAINKM DLVDYSEETF TRIREDYLTF AGQLPGNLDI
210 220 230 240 250
RFVPLSALEG DNVASQSESM PWYSGPTLLE VLETVEIQRV VDAQPMRFPV
260 270 280 290 300
QYVNRPNLDF RGYAGTLASG RVEVGQRVKV LPSGVESNVA RIVTFDGDRE
310 320 330 340 350
EAFAGEAITL VLTDEIDISR GDLLLAADEA LPAVQSASVD VVWMAEQPLS
360 370 380 390 400
PGQSYDIKIA GKKTRARVDG IRYQVDINNL TQREVENLPL NGIGLVDLTF
410 420 430 440 450
DEPLVLDRYQ QNPVTGGLIF IDRLSNVTVG AGMVHEPVSQ ATAAPSEFSA
460 470
FELELNALVR RHFPHWGARD LLGDK
Length:475
Mass (Da):52,558
Last modified:November 1, 1991 - v1
Checksum:i91E983E49732AA15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74586 Genomic DNA. Translation: AAA23646.1.
U29579 Genomic DNA. Translation: AAA69261.1.
U00096 Genomic DNA. Translation: AAC75793.1.
AP009048 Genomic DNA. Translation: BAE76828.1.
PIRiJN0327.
RefSeqiNP_417231.1. NC_000913.3.
WP_001090361.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75793; AAC75793; b2751.
BAE76828; BAE76828; BAE76828.
GeneIDi947219.
KEGGiecj:JW2721.
eco:b2751.
PATRICi32120908. VBIEscCol129921_2846.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74586 Genomic DNA. Translation: AAA23646.1.
U29579 Genomic DNA. Translation: AAA69261.1.
U00096 Genomic DNA. Translation: AAC75793.1.
AP009048 Genomic DNA. Translation: BAE76828.1.
PIRiJN0327.
RefSeqiNP_417231.1. NC_000913.3.
WP_001090361.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP23845.
SMRiP23845. Positions 22-434.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262932. 18 interactions.
DIPiDIP-513N.
IntActiP23845. 3 interactions.
MINTiMINT-1247685.
STRINGi511145.b2751.

Proteomic databases

PaxDbiP23845.
PRIDEiP23845.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75793; AAC75793; b2751.
BAE76828; BAE76828; BAE76828.
GeneIDi947219.
KEGGiecj:JW2721.
eco:b2751.
PATRICi32120908. VBIEscCol129921_2846.

Organism-specific databases

EchoBASEiEB0191.
EcoGeneiEG10194. cysN.

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG2895. LUCA.
HOGENOMiHOG000229289.
InParanoidiP23845.
KOiK00956.
OMAiRGNMLVS.
OrthoDBiEOG6GR38Q.
PhylomeDBiP23845.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.
BioCyciEcoCyc:CYSN-MONOMER.
ECOL316407:JW2721-MONOMER.
MetaCyc:CYSN-MONOMER.
SABIO-RKP23845.

Miscellaneous databases

PROiP23845.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00062. Sulf_adenylyltr_sub1.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence of the sulfate activation locus from Escherichia coli K-12."
    Leyh T.S., Vogt T.F., Suo Y.
    J. Biol. Chem. 267:10405-10410(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The sulfate activation locus of Escherichia coli K12: cloning, genetic, and enzymatic characterization."
    Leyh T.S., Taylor J.C., Markham G.D.
    J. Biol. Chem. 263:2409-2416(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiCYSN_ECOLI
AccessioniPrimary (citable) accession number: P23845
Secondary accession number(s): Q2MA78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: February 17, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.