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Protein

Periplasmic oligopeptide-binding protein

Gene

oppA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity.

GO - Molecular functioni

GO - Biological processi

  • oligopeptide transport Source: EcoliWiki
  • protein transport Source: UniProtKB-KW
  • response to heat Source: EcoCyc
  • transmembrane transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Peptide transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:OPPA-MONOMER.
ECOL316407:JW1235-MONOMER.

Protein family/group databases

TCDBi3.A.1.5.41. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic oligopeptide-binding protein
Gene namesi
Name:oppA
Ordered Locus Names:b1243, JW1235
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10674. oppA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 263 PublicationsAdd BLAST26
ChainiPRO_000003179727 – 543Periplasmic oligopeptide-binding proteinAdd BLAST517

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi297 ↔ 443By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP23843.
PaxDbiP23843.
PRIDEiP23843.

2D gel databases

SWISS-2DPAGEP23843.

Interactioni

Protein-protein interaction databases

BioGridi4263482. 633 interactors.
DIPiDIP-10405N.
IntActiP23843. 2 interactors.
STRINGi511145.b1243.

Structurei

Secondary structure

1543
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi40 – 44Combined sources5
Turni54 – 56Combined sources3
Helixi60 – 69Combined sources10
Beta strandi73 – 76Combined sources4
Beta strandi82 – 93Combined sources12
Turni94 – 96Combined sources3
Beta strandi97 – 102Combined sources6
Helixi116 – 127Combined sources12
Turni129 – 131Combined sources3
Helixi136 – 142Combined sources7
Helixi147 – 151Combined sources5
Helixi157 – 159Combined sources3
Beta strandi160 – 166Combined sources7
Beta strandi169 – 176Combined sources8
Helixi181 – 186Combined sources6
Helixi188 – 190Combined sources3
Helixi195 – 201Combined sources7
Helixi202 – 204Combined sources3
Turni208 – 210Combined sources3
Beta strandi215 – 223Combined sources9
Turni224 – 226Combined sources3
Beta strandi227 – 232Combined sources6
Helixi239 – 241Combined sources3
Beta strandi245 – 250Combined sources6
Helixi255 – 263Combined sources9
Helixi276 – 285Combined sources10
Helixi287 – 289Combined sources3
Beta strandi290 – 303Combined sources14
Turni308 – 311Combined sources4
Helixi313 – 322Combined sources10
Helixi325 – 330Combined sources6
Turni331 – 333Combined sources3
Beta strandi341 – 344Combined sources4
Helixi357 – 360Combined sources4
Helixi363 – 376Combined sources14
Beta strandi381 – 383Combined sources3
Beta strandi386 – 393Combined sources8
Helixi395 – 412Combined sources18
Beta strandi415 – 421Combined sources7
Helixi423 – 432Combined sources10
Beta strandi436 – 443Combined sources8
Beta strandi445 – 448Combined sources4
Helixi450 – 453Combined sources4
Helixi454 – 456Combined sources3
Helixi470 – 479Combined sources10
Beta strandi482 – 484Combined sources3
Helixi485 – 501Combined sources17
Beta strandi505 – 517Combined sources13
Beta strandi521 – 523Combined sources3
Helixi535 – 537Combined sources3
Beta strandi539 – 541Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TCFX-ray2.00A/B/C/D/E/F/G/H27-543[»]
3TCGX-ray2.00A/B/C/D/E/F/G/H27-543[»]
3TCHX-ray1.98A27-543[»]
ProteinModelPortaliP23843.
SMRiP23843.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23843.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108J59. Bacteria.
COG4166. LUCA.
HOGENOMiHOG000179191.
InParanoidiP23843.
KOiK15580.
OMAiMSIITKK.
PhylomeDBiP23843.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR023765. SBP_5_CS.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
PROSITEiPS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNITKRSLV AAGVLAALMA GNVALAADVP AGVTLAEKQT LVRNNGSEVQ
60 70 80 90 100
SLDPHKIEGV PESNISRDLF EGLLVSDLDG HPAPGVAESW DNKDAKVWTF
110 120 130 140 150
HLRKDAKWSD GTPVTAQDFV YSWQRSVDPN TASPYASYLQ YGHIAGIDEI
160 170 180 190 200
LEGKKPITDL GVKAIDDHTL EVTLSEPVPY FYKLLVHPST SPVPKAAIEK
210 220 230 240 250
FGEKWTQPGN IVTNGAYTLK DWVVNERIVL ERSPTYWNNA KTVINQVTYL
260 270 280 290 300
PIASEVTDVN RYRSGEIDMT NNSMPIELFQ KLKKEIPDEV HVDPYLCTYY
310 320 330 340 350
YEINNQKPPF NDVRVRTALK LGMDRDIIVN KVKAQGNMPA YGYTPPYTDG
360 370 380 390 400
AKLTQPEWFG WSQEKRNEEA KKLLAEAGYT ADKPLTINLL YNTSDLHKKL
410 420 430 440 450
AIAASSLWKK NIGVNVKLVN QEWKTFLDTR HQGTFDVARA GWCADYNEPT
460 470 480 490 500
SFLNTMLSNS SMNTAHYKSP AFDSIMAETL KVTDEAQRTA LYTKAEQQLD
510 520 530 540
KDSAIVPVYY YVNARLVKPW VGGYTGKDPL DNTYTRNMYI VKH
Length:543
Mass (Da):60,899
Last modified:October 1, 1994 - v2
Checksum:iBBEF9FEBD42254EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti271N → Y in AAA21302 (Ref. 2) Curated1
Sequence conflicti314 – 315RV → LW in AAA21302 (Ref. 2) Curated2
Sequence conflicti487 – 488QR → HG in AAA21302 (Ref. 2) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05433 Genomic DNA. Translation: AAA21302.1.
M60918 Genomic DNA. Translation: AAB00918.1.
U00096 Genomic DNA. Translation: AAC74325.1.
AP009048 Genomic DNA. Translation: BAA14775.1.
X59501 Genomic DNA. Translation: CAA42089.1.
D83137 Genomic DNA. Translation: BAA11814.1.
PIRiF64871.
RefSeqiNP_415759.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74325; AAC74325; b1243.
BAA14775; BAA14775; BAA14775.
GeneIDi945830.
KEGGiecj:JW1235.
eco:b1243.
PATRICi32117746. VBIEscCol129921_1293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05433 Genomic DNA. Translation: AAA21302.1.
M60918 Genomic DNA. Translation: AAB00918.1.
U00096 Genomic DNA. Translation: AAC74325.1.
AP009048 Genomic DNA. Translation: BAA14775.1.
X59501 Genomic DNA. Translation: CAA42089.1.
D83137 Genomic DNA. Translation: BAA11814.1.
PIRiF64871.
RefSeqiNP_415759.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TCFX-ray2.00A/B/C/D/E/F/G/H27-543[»]
3TCGX-ray2.00A/B/C/D/E/F/G/H27-543[»]
3TCHX-ray1.98A27-543[»]
ProteinModelPortaliP23843.
SMRiP23843.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263482. 633 interactors.
DIPiDIP-10405N.
IntActiP23843. 2 interactors.
STRINGi511145.b1243.

Protein family/group databases

TCDBi3.A.1.5.41. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP23843.

Proteomic databases

EPDiP23843.
PaxDbiP23843.
PRIDEiP23843.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74325; AAC74325; b1243.
BAA14775; BAA14775; BAA14775.
GeneIDi945830.
KEGGiecj:JW1235.
eco:b1243.
PATRICi32117746. VBIEscCol129921_1293.

Organism-specific databases

EchoBASEiEB0668.
EcoGeneiEG10674. oppA.

Phylogenomic databases

eggNOGiENOG4108J59. Bacteria.
COG4166. LUCA.
HOGENOMiHOG000179191.
InParanoidiP23843.
KOiK15580.
OMAiMSIITKK.
PhylomeDBiP23843.

Enzyme and pathway databases

BioCyciEcoCyc:OPPA-MONOMER.
ECOL316407:JW1235-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP23843.
PROiP23843.

Family and domain databases

InterProiIPR030678. Peptide/Ni-bd.
IPR023765. SBP_5_CS.
IPR000914. SBP_5_dom.
[Graphical view]
PfamiPF00496. SBP_bac_5. 1 hit.
[Graphical view]
PIRSFiPIRSF002741. MppA. 1 hit.
PROSITEiPS01040. SBP_BACTERIAL_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOPPA_ECOLI
AccessioniPrimary (citable) accession number: P23843
Secondary accession number(s): P76829
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.