P23842 (YFEA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized protein YfeA

Gene names

Name:	yfeA
Ordered Locus Names:	b2395, JW5391

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	729 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Induction	Expressed during exponential and post-exponential growth at both 28 and 37 degrees Celsius. Repressed by RpoS. Ref.5
Sequence similarities	Contains 1 EAL domain. Contains 1 GGDEF domain.
Sequence caution	The sequence AAA65719.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAA65719.1 differs from that shown. Reason: Frameshift at position 112. The sequence CAA45395.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence CAA45395.1 differs from that shown. Reason: Frameshift at position 112.

Ontologies

Keywords
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cyclic nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro intracellular signal transduction Inferred from electronic annotation. Source: InterPro
Molecular_function	phosphorus-oxygen lyase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 729	729	Uncharacterized protein YfeA		PRO_0000169216
Regions
	Domain	348 – 476	129	GGDEF
	Domain	488 – 729	242	EAL

Sequences

	Sequence		Length	Mass (Da)
	P23842 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: F1CF1AA15D592650	FASTA	729	83,524
	10 20 30 40 50 60 MFVEHNLIKN IKIFTLAFTL TVVLIQLSRF ISPLAIIHSS YIFLAWMPLC VMLSILFIFG 70 80 90 100 110 120 WRGVVPVLCG MFCTNLWNFH LSFLQTAVML GSQTFVVLCA CAILRWQLGT RWRYGLTSRY 130 140 150 160 170 180 VWQRLFWLGL VTPIGIKCSM YLVGSFFDFP LKISTFFGDA DAIFTVVDLL SLFTAVLIYN 190 200 210 220 230 240 MLFYYLTRMI VSPHFAQILW RRDIAPSLGK EKRAFTLSWL AALSVLLLLL CTPYENDFIA 250 260 270 280 290 300 GYLVPVFFII FTLGVGKLRY PFLNLTWAVS TLCLLNYNQN FLQGVETEYS LAFILAVLIS 310 320 330 340 350 360 FSVCLLYMVR IYHRSEWLNR RWHLQALTDP LTLLPNFRAL EQAPEQEAGK SFCCLRIDNL 370 380 390 400 410 420 EFMSRHYGLM MRVHCIRSIC RTLLPLMQEN EKLYQLPGSE LLLVLSGPET EGRLQHMVNI 430 440 450 460 470 480 LNSRQIHWNN TGLDMGYGAA WGRFDGNQET LQPLLGQLSW LAEQSCAHHH VLALDSREEM 490 500 510 520 530 540 VSGQTTKQVL LLNTIRTALD QGDLLLYAQP IRNKEGEGYD EILARLKYDG GIMTPDKFLP 550 560 570 580 590 600 LIAQFNLSAR FDLQVLESLL KWLATHPCDK KGPRFSVNLM PLTLLQKNIA GRIIRLFKRY 610 620 630 640 650 660 HISPQAVILE ITEEQAFSNA ESSMYNIEQL HKFGFRIAID DFGTGYANYE RLKRLQADII 670 680 690 700 710 720 KIDGVFVKDI VTNTLDAMIV RSITDLAKAK SLSVVAEFVE TQQQQALLHK LGVQYLQGYL IGRPQPLAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Precise mapping and comparison of two evolutionarily related regions of the Escherichia coli K-12 chromosome. Evolution of valU and lysT from an ancestral tRNA operon." Brun Y.V., Breton R., Lanouette P., Lapointe J. J. Mol. Biol. 214:825-843(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-153. Strain: K12.
[5]	"Gene expression patterns and differential input into curli fimbriae regulation of all GGDEF/EAL domain proteins in Escherichia coli." Sommerfeldt N., Possling A., Becker G., Pesavento C., Tschowri N., Hengge R. Microbiology 155:1318-1331(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, RPOS-REPRESSION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC75454.2. AP009048 Genomic DNA. Translation: BAA16267.2. M13687 Genomic DNA. Translation: AAA65719.1. Sequence problems. X63976 Genomic DNA. Translation: CAA45395.1. Sequence problems.
PIR	H65013. S11411.
RefSeq	NP_416896.4. NC_000913.2. YP_490636.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P23842.
SMR	P23842. Positions 498-726.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12011N.
IntAct	P23842. 1 interaction.
MINT	MINT-1223642.
STRING	511145.b2395.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75454; AAC75454; b2395. BAA16267; BAA16267; BAA16267.
GeneID	12930192. 946864.
KEGG	ecj:Y75_p2361. eco:b2395.
PATRIC	32120167. VBIEscCol129921_2492.
Organism-specific databases
EchoBASE	EB1135.
EcoGene	EG11145. yfeA.
Phylogenomic databases
eggNOG	COG2199.
HOGENOM	HOG000122953.
OMA	AQFNLSV.
ProtClustDB	CLSK880383.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11145-MONOMER. ECOL316407:JW5391-MONOMER.
Gene expression databases
Genevestigator	P23842.
Family and domain databases
Gene3D	3.20.20.450. 1 hit.
InterPro	IPR001054. A/G_cyclase. IPR001633. Diguanylate_PEstase_EAL_dom. IPR000160. GGDEF_dom. IPR007895. MASE1. [Graphical view]
Pfam	PF00563. EAL. 1 hit. PF05231. MASE1. 1 hit. [Graphical view]
SMART	SM00052. EAL. 1 hit. SM00267. GGDEF. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 1 hit. SSF141868. SSF141868. 1 hit.
PROSITE	PS50883. EAL. 1 hit. PS50887. GGDEF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YFEA_ECOLI

Accession

Primary (citable) accession number: P23842
Secondary accession number(s): P76529, P76954

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	April 27, 2001
Last modified:	May 1, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program