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P23836

- PHOP_ECOLI

UniProt

P23836 - PHOP_ECOLI

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Protein

Transcriptional regulatory protein PhoP

Gene

phoP

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system PhoP/PhoQ involved in adaptation to low Mg2+ environments and the control of acid resistance genes. In low periplasmic Mg2+, PhoQ phosphorylates PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg2+, PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG (By similarity). Mediates magnesium influx to the cytosol by activation of MgtA. Promotes expression of the two-component regulatory system rstA/rstB and transcription of the hemL, mgrB, nagA, slyB, vboR and yrbL genes.By similarity1 Publication

Enzyme regulationi

Feedback inhibited by MgrB, which seems to bind PhoQ, altering its activity and that of downstream effector PhoP. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, or treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.2 Publications

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. identical protein binding Source: IntAct

GO - Biological processi

  1. phosphorelay signal transduction system Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PHOP-MONOMER.
ECOL316407:JW1116-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein PhoP
Gene namesi
Name:phoP
Ordered Locus Names:b1130, JW1116
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10731. phoP.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Altered expression of 33 genes; 9 are down-regulated, the rest up-regulated.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Transcriptional regulatory protein PhoPPRO_0000081195Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 5114-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by PhoQ.Curated

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP23836.
PRIDEiP23836.

Expressioni

Inductioni

The phoP/phoQ operon is positively autoregulated by both PhoP and PhoQ in a Mg2+-dependent manner, inhibited at high Mg2+ concentrations (PubMed:10464230). Induced by dsbA disruption and dithiothreitol (PubMed:22267510).2 Publications

Gene expression databases

GenevestigatoriP23836.

Interactioni

Subunit structurei

Monomer in the inactive, unphosphorylated state and dimer in the active, phosphorylated state.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself5EBI-1121453,EBI-1121453

Protein-protein interaction databases

DIPiDIP-10500N.
IntActiP23836. 5 interactions.
STRINGi511145.b1130.

Structurei

Secondary structure

1
223
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Helixi10 – 2213Combined sources
Beta strandi26 – 327Combined sources
Helixi33 – 4210Combined sources
Beta strandi46 – 505Combined sources
Beta strandi55 – 573Combined sources
Helixi59 – 6810Combined sources
Beta strandi75 – 806Combined sources
Helixi84 – 929Combined sources
Beta strandi96 – 1027Combined sources
Helixi105 – 11915Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PKXX-ray2.54A/B1-121[»]
2PL1X-ray1.90A1-121[»]
ProteinModelPortaliP23836.
SMRiP23836. Positions 1-221.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23836.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 116116Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034815.
InParanoidiP23836.
KOiK07660.
OMAiVELPVIM.
OrthoDBiEOG6G4VQG.
PhylomeDBiP23836.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23836-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVLVVEDNA LLRHHLKVQI QDAGHQVDDA EDAKEADYYL NEHIPDIAIV
60 70 80 90 100
DLGLPDEDGL SLIRRWRSND VSLPILVLTA RESWQDKVEV LSAGADDYVT
110 120 130 140 150
KPFHIEEVMA RMQALMRRNS GLASQVISLP PFQVDLSRRE LSINDEVIKL
160 170 180 190 200
TAFEYTIMET LIRNNGKVVS KDSLMLQLYP DAELRESHTI DVLMGRLRKK
210 220
IQAQYPQEVI TTVRGQGYLF ELR
Length:223
Mass (Da):25,535
Last modified:November 1, 1991 - v1
Checksum:i2EFF27E3923D43EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90393 Genomic DNA. Translation: BAA14390.1.
M81433 Genomic DNA. Translation: AAA24377.1.
U00096 Genomic DNA. Translation: AAC74214.1.
AP009048 Genomic DNA. Translation: BAA35952.1.
M74924 Genomic DNA. Translation: AAA92732.1.
X59307 Genomic DNA. Translation: CAA41997.1.
PIRiA41965.
RefSeqiNP_415648.1. NC_000913.3.
YP_489398.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74214; AAC74214; b1130.
BAA35952; BAA35952; BAA35952.
GeneIDi12931100.
945697.
KEGGiecj:Y75_p1100.
eco:b1130.
PATRICi32117509. VBIEscCol129921_1177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90393 Genomic DNA. Translation: BAA14390.1 .
M81433 Genomic DNA. Translation: AAA24377.1 .
U00096 Genomic DNA. Translation: AAC74214.1 .
AP009048 Genomic DNA. Translation: BAA35952.1 .
M74924 Genomic DNA. Translation: AAA92732.1 .
X59307 Genomic DNA. Translation: CAA41997.1 .
PIRi A41965.
RefSeqi NP_415648.1. NC_000913.3.
YP_489398.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2PKX X-ray 2.54 A/B 1-121 [» ]
2PL1 X-ray 1.90 A 1-121 [» ]
ProteinModelPortali P23836.
SMRi P23836. Positions 1-221.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10500N.
IntActi P23836. 5 interactions.
STRINGi 511145.b1130.

Proteomic databases

PaxDbi P23836.
PRIDEi P23836.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74214 ; AAC74214 ; b1130 .
BAA35952 ; BAA35952 ; BAA35952 .
GeneIDi 12931100.
945697.
KEGGi ecj:Y75_p1100.
eco:b1130.
PATRICi 32117509. VBIEscCol129921_1177.

Organism-specific databases

EchoBASEi EB0724.
EcoGenei EG10731. phoP.

Phylogenomic databases

eggNOGi COG0745.
HOGENOMi HOG000034815.
InParanoidi P23836.
KOi K07660.
OMAi VELPVIM.
OrthoDBi EOG6G4VQG.
PhylomeDBi P23836.

Enzyme and pathway databases

BioCyci EcoCyc:PHOP-MONOMER.
ECOL316407:JW1116-MONOMER.

Miscellaneous databases

EvolutionaryTracei P23836.
PROi P23836.

Gene expression databases

Genevestigatori P23836.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view ]
SMARTi SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52172. SSF52172. 1 hit.
PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular analysis of the Escherichia coli phoP-phoQ operon."
    Kasahara M., Nakata A., Shinagawa H.
    J. Bacteriol. 174:492-498(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Molecular genetic analysis of the Escherichia coli phoP locus."
    Groisman E.A., Heffron F., Solomon F.
    J. Bacteriol. 174:486-491(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR."
    He B., Smith J.M., Zalkin H.
    J. Bacteriol. 174:130-136(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
    Strain: K12.
  7. Green S.M., Drabble W.T.
    Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
    Strain: K12.
  8. "Novel mode of transcription regulation of divergently overlapping promoters by PhoP, the regulator of two-component system sensing external magnesium availability."
    Yamamoto K., Ogasawara H., Fujita N., Utsumi R., Ishihama A.
    Mol. Microbiol. 45:423-438(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-7 AND 119-125.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  9. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  10. "Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12: identification of extracellular Mg2+-responsive promoters."
    Kato A., Tanabe H., Utsumi R.
    J. Bacteriol. 181:5516-5520(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY LOW MG(2+).
    Strain: K12 / MC4100 / JA176.
  11. "Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli."
    Minagawa S., Ogasawara H., Kato A., Yamamoto K., Eguchi Y., Oshima T., Mori H., Ishihama A., Utsumi R.
    J. Bacteriol. 185:3696-3702(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME-WIDE ANALYSIS, REGULATION BY MG(2+).
    Strain: K12 / MC4100 / JA176.
  12. "Genome-wide analyses revealing a signaling network of the RcsC-YojN-RcsB phosphorelay system in Escherichia coli."
    Hagiwara D., Sugiura M., Oshima T., Mori H., Aiba H., Yamashino T., Mizuno T.
    J. Bacteriol. 185:5735-5746(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME-WIDE ANALYSIS.
    Strain: K12 / ST001.
  13. "Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli."
    Eguchi Y., Okada T., Minagawa S., Oshima T., Mori H., Yamamoto K., Ishihama A., Utsumi R.
    J. Bacteriol. 186:3006-3014(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EVGA/EVGS.
    Strain: K12 / MC4100 / JA176.
  14. "Dissecting the PhoP regulatory network of Escherichia coli and Salmonella enterica."
    Zwir I., Shin D., Kato A., Nishino K., Latifi T., Solomon F., Hare J.M., Huang H., Groisman E.A.
    Proc. Natl. Acad. Sci. U.S.A. 102:2862-2867(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ANALYSIS OF PHOP REGULATORY NETWORK, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.
  15. "Feedback inhibition in the PhoQ/PhoP signaling system by a membrane peptide."
    Lippa A.M., Goulian M.
    PLoS Genet. 5:E1000788-E1000788(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
    Strain: K12 / MG1655 / ATCC 47076.
  16. "Perturbation of the oxidizing environment of the periplasm stimulates the PhoQ/PhoP system in Escherichia coli."
    Lippa A.M., Goulian M.
    J. Bacteriol. 194:1457-1463(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION, INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  17. "Crystal structures of the receiver domain of the response regulator PhoP from Escherichia coli in the absence and presence of the phosphoryl analog beryllofluoride."
    Bachhawat P., Stock A.M.
    J. Bacteriol. 189:5987-5995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 1-120 IN THE PRESENCE AND ABSENCE OF PHOSPHORYLATION ANALOG, SUBUNIT.

Entry informationi

Entry nameiPHOP_ECOLI
AccessioniPrimary (citable) accession number: P23836
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 26, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

There is a close linkage between the Rcs and PhoQ/P signaling systems, and both signaling systems respond to certain external divalent cations (zinc and magnesium).
Two-component regulatory system EvgA/EvgS interacts with PhoP/PhoQ via signal transduction mediated by phospho-EvgA.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3