Transcriptional regulatory protein PhoP - Escherichia coli (strain K12)

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P23836 (PHOP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 119. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein PhoP

Gene names

Name:	phoP
Ordered Locus Names:	b1130, JW1116

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system PhoQ/PhoP involved in adaptation to low Mg²⁺ environments and the control of acid resistance genes. Mediates magnesium influx to the cytosol by activation of MgtA. Promotes expression of the two-component regulatory system rstA/rstB and transcription of the hemL, mgrB, nagA, slyB, vboR and yrbL genes. Ref.10
Subcellular location	Cytoplasm Probable.
Induction	PhoQ/phoP operon is positively autoregulated by both PhoP and PhoQ in a Mg²⁺-dependent manner. Ref.10 Ref.11
Post-translational modification	Phosphorylated by PhoQ Probable.
Miscellaneous	There is a close linkage between the Rcs and PhoQ/P signaling systems, and both signaling systems respond to certain external divalent cations (zinc and magnesium). Two-component regulatory system EvgA/EvgS interacts with PhoQ/PhoP via signal transduction mediated by phospho-EvgA.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-1121453,EBI-1121453

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 223

223

Transcriptional regulatory protein PhoP

PRO_0000081195

Regions

Domain

1 – 116

116

Response regulatory

Amino acid modifications

Modified residue

4-aspartylphosphate By similarity

Secondary structure

223

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P23836 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 2EFF27E3923D43EF
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FASTA

223

25,535

        10         20         30         40         50         60 
MRVLVVEDNA LLRHHLKVQI QDAGHQVDDA EDAKEADYYL NEHIPDIAIV DLGLPDEDGL 

        70         80         90        100        110        120 
SLIRRWRSND VSLPILVLTA RESWQDKVEV LSAGADDYVT KPFHIEEVMA RMQALMRRNS 

       130        140        150        160        170        180 
GLASQVISLP PFQVDLSRRE LSINDEVIKL TAFEYTIMET LIRNNGKVVS KDSLMLQLYP 

       190        200        210        220 
DAELRESHTI DVLMGRLRKK IQAQYPQEVI TTVRGQGYLF ELR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of the Escherichia coli phoP-phoQ operon." Kasahara M., Nakata A., Shinagawa H. J. Bacteriol. 174:492-498(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Molecular genetic analysis of the Escherichia coli phoP locus." Groisman E.A., Heffron F., Solomon F. J. Bacteriol. 174:486-491(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR." He B., Smith J.M., Zalkin H. J. Bacteriol. 174:130-136(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50. Strain: K12.
[7]	Green S.M., Drabble W.T. Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24. Strain: K12.
[8]	"Novel mode of transcription regulation of divergently overlapping promoters by PhoP, the regulator of two-component system sensing external magnesium availability." Yamamoto K., Ogasawara H., Fujita N., Utsumi R., Ishihama A. Mol. Microbiol. 45:423-438(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-7 AND 119-125. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[9]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
[10]	"Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12: identification of extracellular Mg2+-responsive promoters." Kato A., Tanabe H., Utsumi R. J. Bacteriol. 181:5516-5520(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, REGULATION. Strain: K12 / MC4100 / JA176.
[11]	"Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli." Minagawa S., Ogasawara H., Kato A., Yamamoto K., Eguchi Y., Oshima T., Mori H., Ishihama A., Utsumi R. J. Bacteriol. 185:3696-3702(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME-WIDE ANALYSIS, REGULATION BY MG(2+). Strain: K12 / MC4100 / JA176.
[12]	"Genome-wide analyses revealing a signaling network of the RcsC-YojN-RcsB phosphorelay system in Escherichia coli." Hagiwara D., Sugiura M., Oshima T., Mori H., Aiba H., Yamashino T., Mizuno T. J. Bacteriol. 185:5735-5746(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME-WIDE ANALYSIS. Strain: K12 / ST001.
[13]	"Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli." Eguchi Y., Okada T., Minagawa S., Oshima T., Mori H., Yamamoto K., Ishihama A., Utsumi R. J. Bacteriol. 186:3006-3014(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH EVGA/EVGS. Strain: K12 / MC4100 / JA176.
[14]	"Dissecting the PhoP regulatory network of Escherichia coli and Salmonella enterica." Zwir I., Shin D., Kato A., Nishino K., Latifi T., Solomon F., Hare J.M., Huang H., Groisman E.A. Proc. Natl. Acad. Sci. U.S.A. 102:2862-2867(2005) [PubMed] [Europe PMC] [Abstract] Cited for: ANALYSIS OF PHOP REGULATORY NETWORK. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D90393 Genomic DNA. Translation: BAA14390.1.
M81433 Genomic DNA. Translation: AAA24377.1.
U00096 Genomic DNA. Translation: AAC74214.1.
AP009048 Genomic DNA. Translation: BAA35952.1.
M74924 Genomic DNA. Translation: AAA92732.1.
X59307 Genomic DNA. Translation: CAA41997.1.

PIR

A41965.

RefSeq

NP_415648.1. NC_000913.2.
YP_489398.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PKX	X-ray	2.54	A/B	1-120	[»]
2PL1	X-ray	1.90	A	1-120	[»]

ProteinModelPortal

P23836.

SMR

P23836. Positions 1-221.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10500N.

IntAct

P23836. 5 interactions.

STRING

511145.b1130.

Proteomic databases

PaxDb

P23836.

PRIDE

P23836.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74214; AAC74214; b1130.
BAA35952; BAA35952; BAA35952.

GeneID

12931100.
945697.

KEGG

ecj:Y75_p1100.
eco:b1130.

PATRIC

32117509. VBIEscCol129921_1177.

Organism-specific databases

EchoBASE

EB0724.

EcoGene

EG10731. phoP.

Phylogenomic databases

eggNOG

COG0745.

HOGENOM

HOG000034815.

K07660.

OMA

ADYFLNE.

ProtClustDB

PRK10816.

Enzyme and pathway databases

BioCyc

EcoCyc:PHOP-MONOMER.
ECOL316407:JW1116-MONOMER.

Gene expression databases

Genevestigator

P23836.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]

SMART

SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]

SUPFAM

SSF52172. CheY_like. 1 hit.

PROSITE

PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P23836.

Entry information

Entry name

PHOP_ECOLI

Accession

Primary (citable) accession number: P23836

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	May 1, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents