P23833 (SCO1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SCO1, mitochondrial

Gene names

Name:	SCO1
Ordered Locus Names:	YBR037C
ORF Names:	YBR0406

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the accumulation of subunits 1 and 2 of cytochrome c oxidase complex. Thought to play a role in either mitochondrial copper transport or insertion of copper into the active site of COX. Ref.7
Subcellular location	Mitochondrion inner membrane Ref.5.
Miscellaneous	Present with 2550 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the SCO1/2 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane Transmembrane helix
Ligand	Copper Metal-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular copper ion homeostasis Inferred from electronic annotation. Source: InterPro copper ion transport Inferred from genetic interaction. Source: SGD respiratory chain complex IV assembly Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from direct assay Ref.5. Source: SGD
Molecular function	copper ion binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 295

Protein SCO1, mitochondrial

PRO_0000031923

Regions

Transmembrane

76 – 92

17

Helical; Potential

Sites

Metal binding

148

1

Copper By similarity

Metal binding

152

1

Copper By similarity

Metal binding

239

1

Copper By similarity

Secondary structure

1

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295

Helix Strand Turn

Details...

Beta strand

120 – 123

4

Beta strand

128 – 130

3

Helix

131 – 134

4

Beta strand

139 – 144

6

Helix

151 – 170

20

Beta strand

175 – 181

7

Turn

183 – 185

3

Helix

188 – 194

7

Helix

195 – 197

3

Beta strand

203 – 206

4

Helix

209 – 218

10

Beta strand

243 – 246

4

Beta strand

252 – 256

5

Helix

264 – 275

12

Beta strand

284 – 291

8

Sequences

	Sequence		Length	Mass (Da)
	P23833 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 500EFD92A0F44DDA	FASTA	295	33,166
	10 20 30 40 50 60 MLKLSRSANL RLVQLPAARL SGNGAKLLTQ RGFFTVTRLW QSNGKKPLSR VPVGGTPIKD 70 80 90 100 110 120 NGKVREGSIE FSTGKAIALF LAVGGALSYF FNREKRRLET QKEAEANRGY GKPSLGGPFH 130 140 150 160 170 180 LEDMYGNEFT EKNLLGKFSI IYFGFSNCPD ICPDELDKLG LWLNTLSSKY GITLQPLFIT 190 200 210 220 230 240 CDPARDSPAV LKEYLSDFHP SILGLTGTFD EVKNACKKYR VYFSTPPNVK PGQDYLVDHS 250 260 270 280 290 IFFYLMDPEG QFVDALGRNY DEKTGVDKIV EHVKSYVPAE QRAKQKEAWY SFLFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Accumulation of the cytochrome c oxidase subunits I and II in yeast requires a mitochondrial membrane-associated protein, encoded by the nuclear SCO1 gene." Schulze M., Roedel G. Mol. Gen. Genet. 216:37-43(1989) [PubMed: 2543907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Immunological identification of yeast SCO1 protein as a component of the inner mitochondrial membrane." Buchwald P., Krummeck G., Roedel G. Mol. Gen. Genet. 229:413-420(1991) [PubMed: 1944230] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Crystal structure of yeast Sco1." Abajian C., Rosenzweig A.C. J. Biol. Inorg. Chem. 11:459-466(2006) [PubMed: 16570183] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 96-295 IN COMPLEX WITH COPPER IONS, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X17441 Genomic DNA. Translation: CAA35490.1.
Z35906 Genomic DNA. Translation: CAA84979.1.
AY557863 Genomic DNA. Translation: AAS56189.1.
BK006936 Genomic DNA. Translation: DAA07157.1.

PIR

JE0051.

RefSeq

NP_009593.1. NM_001178385.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2B7J	X-ray	2.30	A/B/C/D	96-295	[»]
2B7K	X-ray	1.80	A/B/C/D	96-295	[»]

ProteinModelPortal

P23833.

SMR

P23833. Positions 113-293.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-7610N.

IntAct

P23833. 3 interactions.

STRING

P23833.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YBR037C; YBR037C; YBR037C.

GeneID

852325.

KEGG

sce:YBR037C.

NMPDR

fig|4932.3.peg.288.

Organism-specific databases

CYGD

YBR037c.

SGD

S000000241. SCO1.

Phylogenomic databases

eggNOG

fuNOG04530.

GeneTree

EFGT00050000005486.

HOGENOM

HBG756383.

OMA

GNEFTEK.

OrthoDB

EOG40VZZP.

Gene expression databases

ArrayExpress

P23833.

Genevestigator

P23833.

GermOnline

YBR037C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR003782. Cu-chaperone_SCO1/SenC.
IPR017276. Synth_of_cyt-c-oxidase_Sco1/2.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

Gene3D

G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

KO

K07152.

PANTHER

PTHR12151. SCO1_SenC. 1 hit.

Pfam

PF02630. SCO1-SenC. 1 hit.
[Graphical view]

PIRSF

PIRSF037736. SCO1. 1 hit.

SUPFAM

SSF52833. Thiordxn-like_fd. 1 hit.

ProtoNet

Search...

Other

NextBio

971029.

Entry information

Entry name

SCO1_YEAST

Accession

Primary (citable) accession number: P23833
Secondary accession number(s): D6VQ37

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	December 14, 2011
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program