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Protein

CDP-diacylglycerol--serine O-phosphatidyltransferase

Gene

pssA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine.

GO - Molecular functioni

  • CDP-diacylglycerol-serine O-phosphatidyltransferase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciEcoCyc:PHOSPHASERSYN-MONOMER.
ECOL316407:JW2569-MONOMER.
MetaCyc:PHOSPHASERSYN-MONOMER.
BRENDAi2.7.8.8. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
CDP-diacylglycerol--serine O-phosphatidyltransferase (EC:2.7.8.8)
Alternative name(s):
Phosphatidylserine synthase
Gene namesi
Name:pssA
Synonyms:pss
Ordered Locus Names:b2585, JW2569
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10781. pssA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451CDP-diacylglycerol--serine O-phosphatidyltransferasePRO_0000056824Add
BLAST

Proteomic databases

EPDiP23830.
PaxDbiP23830.
PRIDEiP23830.

Interactioni

Subunit structurei

Multimeric. Interacts with ACP, YbgC and PlsB, forming altogether a complex at the inner membrane.1 Publication

Protein-protein interaction databases

BioGridi4259429. 210 interactions.
DIPiDIP-10593N.
IntActiP23830. 35 interactions.
MINTiMINT-1218158.
STRINGi511145.b2585.

Structurei

3D structure databases

ProteinModelPortaliP23830.
SMRiP23830. Positions 6-451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini133 – 15927PLD phosphodiesterase 1PROSITE-ProRule annotationAdd
BLAST
Domaini352 – 37928PLD phosphodiesterase 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 15436HydrophobicAdd
BLAST
Regioni239 – 28446HydrophobicAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 96Lys-rich (basic)
Compositional biasi430 – 44920Arg/Lys-rich (basic)Add
BLAST

Sequence similaritiesi

Contains 2 PLD phosphodiesterase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105JIM. Bacteria.
COG1502. LUCA.
HOGENOMiHOG000270167.
InParanoidiP23830.
KOiK00998.
OMAiHLKGFVI.
OrthoDBiEOG6M3P98.
PhylomeDBiP23830.

Family and domain databases

InterProiIPR025202. PLD-like_dom.
IPR016270. PLipase-D_PtdSer-synthase-type.
IPR001736. PLipase_D/transphosphatidylase.
[Graphical view]
PANTHERiPTHR12586. PTHR12586. 1 hit.
PfamiPF13091. PLDc_2. 2 hits.
[Graphical view]
PIRSFiPIRSF000850. Phospholipase_D_PSS. 1 hit.
SMARTiSM00155. PLDc. 2 hits.
[Graphical view]
PROSITEiPS50035. PLD. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23830-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSKFKRNKH QQHLAQLPKI SQSVDDVDFF YAPADFRETL LEKIASAKQR
60 70 80 90 100
ICIVALYLEQ DDGGKGILNA LYEAKRQRPE LDVRVLVDWH RAQRGRIGAA
110 120 130 140 150
ASNTNADWYC RMAQENPGVD VPVYGVPINT REALGVLHFK GFIIDDSVLY
160 170 180 190 200
SGASLNDVYL HQHDKYRYDR YHLIRNRKMS DIMFEWVTQN IMNGRGVNRL
210 220 230 240 250
DDVNRPKSPE IKNDIRLFRQ ELRDAAYHFQ GDADNDQLSV TPLVGLGKSS
260 270 280 290 300
LLNKTIFHLM PCAEQKLTIC TPYFNLPAIL VRNIIQLLRE GKKVEIIVGD
310 320 330 340 350
KTANDFYIPE DEPFKIIGAL PYLYEINLRR FLSRLQYYVN TDQLVVRLWK
360 370 380 390 400
DDDNTYHLKG MWVDDKWMLI TGNNLNPRAW RLDLENAILI HDPQLELAPQ
410 420 430 440 450
REKELELIRE HTTIVKHYRD LQSIADYPVK VRKLIRRLRR IRIDRLISRI

L
Length:451
Mass (Da):52,802
Last modified:November 1, 1997 - v2
Checksum:i9E9A2A5C4B4C814F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321A → R in AAA97504 (PubMed:2002065).Curated
Sequence conflicti78 – 781R → DD in AAA97504 (PubMed:2002065).Curated
Sequence conflicti165 – 1673KYR → NIA in AAA97504 (PubMed:2002065).Curated
Sequence conflicti287 – 2882LL → FV in AAA97504 (PubMed:2002065).Curated
Sequence conflicti309 – 3091P → S in AAA97504 (PubMed:2002065).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58699 Genomic DNA. Translation: AAA97504.1.
U00096 Genomic DNA. Translation: AAC75638.2.
AP009048 Genomic DNA. Translation: BAA16470.2.
RefSeqiNP_417080.4. NC_000913.3.
WP_000949265.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75638; AAC75638; b2585.
BAA16470; BAA16470; BAA16470.
GeneIDi947059.
KEGGiecj:JW2569.
eco:b2585.
PATRICi32120571. VBIEscCol129921_2688.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58699 Genomic DNA. Translation: AAA97504.1.
U00096 Genomic DNA. Translation: AAC75638.2.
AP009048 Genomic DNA. Translation: BAA16470.2.
RefSeqiNP_417080.4. NC_000913.3.
WP_000949265.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP23830.
SMRiP23830. Positions 6-451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259429. 210 interactions.
DIPiDIP-10593N.
IntActiP23830. 35 interactions.
MINTiMINT-1218158.
STRINGi511145.b2585.

Proteomic databases

EPDiP23830.
PaxDbiP23830.
PRIDEiP23830.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75638; AAC75638; b2585.
BAA16470; BAA16470; BAA16470.
GeneIDi947059.
KEGGiecj:JW2569.
eco:b2585.
PATRICi32120571. VBIEscCol129921_2688.

Organism-specific databases

EchoBASEiEB0774.
EcoGeneiEG10781. pssA.

Phylogenomic databases

eggNOGiENOG4105JIM. Bacteria.
COG1502. LUCA.
HOGENOMiHOG000270167.
InParanoidiP23830.
KOiK00998.
OMAiHLKGFVI.
OrthoDBiEOG6M3P98.
PhylomeDBiP23830.

Enzyme and pathway databases

BioCyciEcoCyc:PHOSPHASERSYN-MONOMER.
ECOL316407:JW2569-MONOMER.
MetaCyc:PHOSPHASERSYN-MONOMER.
BRENDAi2.7.8.8. 2026.

Miscellaneous databases

PROiP23830.

Family and domain databases

InterProiIPR025202. PLD-like_dom.
IPR016270. PLipase-D_PtdSer-synthase-type.
IPR001736. PLipase_D/transphosphatidylase.
[Graphical view]
PANTHERiPTHR12586. PTHR12586. 1 hit.
PfamiPF13091. PLDc_2. 2 hits.
[Graphical view]
PIRSFiPIRSF000850. Phospholipase_D_PSS. 1 hit.
SMARTiSM00155. PLDc. 2 hits.
[Graphical view]
PROSITEiPS50035. PLD. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and inactivation of the pss gene of Escherichia coli. Phosphatidylethanolamine may not be essential for cell viability."
    Dechavigny A., Heacock P.N., Dowhan W.
    J. Biol. Chem. 266:5323-5332(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Phosphatidylserine synthase from Escherichia coli."
    Dowhan W.
    Methods Enzymol. 209:287-298(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  6. "A protein network for phospholipid synthesis uncovered by a variant of the tandem affinity purification method in Escherichia coli."
    Gully D., Bouveret E.
    Proteomics 6:282-293(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ACP; YBGC AND PLSB.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPSS_ECOLI
AccessioniPrimary (citable) accession number: P23830
Secondary accession number(s): P78100, P78256
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1997
Last modified: March 16, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.