Reviewed, UniProtKB/Swiss-Prot P23826 (LACS_LACSL)

Names and origin

Protein names

Recommended name:
    Bacteriocin lactocin-S

Gene names

Name:

lasA

Encoded on

Plasmid pCIM1

Organism

Lactobacillus sakei L45

Taxonomic identifier

1634 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	68 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function

Inhibits the growth of selected species of the genera Lactobacillus, Pedicoccus, and Leuconostoc.

Ontologies

Keywords
Ligand	Pyruvate
Molecular function	Antibiotic Antimicrobial Bacteriocin Lantibiotic
PTM	D-amino acid Thioether bond
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	receptor binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 31	31			PRO_0000002764
	Chain	32 – 68	37	Bacteriocin lactocin-S		PRO_0000002765
Amino acid modifications
	Modified residue	32	1	Pyruvic acid (Ser)
	Modified residue	33	1	2,3-didehydrobutyrine
	Modified residue	38	1	D-alanine (Ser)
	Modified residue	42	1	D-alanine (Ser)
	Modified residue	50	1	D-alanine (Ser)
	Cross-link	54 ↔ 59		Lanthionine (Ser-Cys) Probable
	Cross-link	63 ↔ 68		Lanthionine (Ser-Cys) Probable

Sequences

	Sequence		Length	Mass (Da)
	P23826-1 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: 5FD203E8506ADC2C	FASTA	68	7,258
	10 20 30 40 50 60 MKTEKKVLDE LSLHASAKMG ARDVESSMNA DSTPVLASVA VSMELLPTAS VLYSDVAGCF KYSAKHHC

References

[1]	"In vivo conversion of L-serine to D-alanine in a ribosomally synthesized polypeptide." Skaugen M., Nissen-Meyer J., Jung G., Stevanovic S., Sletten K., Inger C., Abildgaard C.I., Nes I.F. J. Biol. Chem. 269:27183-27185(1994) [PubMed: 7961627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Analysis of genes involved in the biosynthesis of the lantibiotic lactocin S." Skaugen M., Abildgaard C.I., Nes I.F. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45." Mortvedt C.I., Nissen-Meyer J., Sletten K., Nes I.F. Appl. Environ. Microbiol. 57:1829-1834(1991) [PubMed: 1872611] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-67.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79889 Genomic DNA. Translation: CAA56261.1. Z54312 Genomic DNA. Translation: CAA91109.1.
PIR	A55457.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	1.C.23.1.1. lactocin S family.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

LACS_LACSL

Accession

Primary (citable) accession number: P23826
Secondary accession number(s): Q48848, Q48858

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	July 19, 2004
Last modified:	March 3, 2009
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)