P23826 (LACS_LACSL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bacteriocin lactocin-S

Gene names

Name:

lasA

Encoded on

Plasmid pCIM1

Organism

Lactobacillus sakei L45

Taxonomic identifier

1634 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	68 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits the growth of selected species of the genera Lactobacillus, Pedicoccus, and Leuconostoc.
Post-translational modification	It is not established whether the 2,3-didehydrobutyrine is the E- or Z-isomer (Ref.1).

Ontologies

Keywords
Ligand	Pyruvate
Molecular function	Antibiotic Antimicrobial Bacteriocin Lantibiotic
PTM	D-amino acid Thioether bond
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	receptor binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 31	31			PRO_0000002764
	Chain	32 – 68	37	Bacteriocin lactocin-S		PRO_0000002765
Amino acid modifications
	Modified residue	32	1	Pyruvic acid (Ser)
	Modified residue	33	1	2,3-didehydrobutyrine
	Modified residue	38	1	D-alanine (Ser)
	Modified residue	42	1	D-alanine (Ser)
	Modified residue	50	1	D-alanine (Ser)
	Cross-link	54 ↔ 59		Lanthionine (Ser-Cys) Probable
	Cross-link	63 ↔ 68		Lanthionine (Ser-Cys) Probable

Sequences

	Sequence		Length	Mass (Da)
	P23826 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: 5FD203E8506ADC2C	FASTA	68	7,258
	10 20 30 40 50 60 MKTEKKVLDE LSLHASAKMG ARDVESSMNA DSTPVLASVA VSMELLPTAS VLYSDVAGCF KYSAKHHC

References

[1]	"In vivo conversion of L-serine to D-alanine in a ribosomally synthesized polypeptide." Skaugen M., Nissen-Meyer J., Jung G., Stevanovic S., Sletten K., Inger C., Abildgaard C.I., Nes I.F. J. Biol. Chem. 269:27183-27185(1994) [PubMed: 7961627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Analysis of genes involved in the biosynthesis of the lantibiotic lactocin S." Skaugen M., Abildgaard C.I., Nes I.F. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Purification and amino acid sequence of lactocin S, a bacteriocin produced by Lactobacillus sake L45." Mortvedt C.I., Nissen-Meyer J., Sletten K., Nes I.F. Appl. Environ. Microbiol. 57:1829-1834(1991) [PubMed: 1872611] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-67.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79889 Genomic DNA. Translation: CAA56261.1. Z54312 Genomic DNA. Translation: CAA91109.1.
PIR	A55457.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	1.C.23.1.1. lactocin S family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

LACS_LACSL

Accession

Primary (citable) accession number: P23826
Secondary accession number(s): Q48848, Q48858

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	July 19, 2004
Last modified:	May 3, 2011
This is version 35 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program