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Protein

Glutamate receptor 1

Gene

Gria1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei464 – 4641GlutamateBy similarity
Binding sitei499 – 4991GlutamateBy similarity
Binding sitei719 – 7191GlutamateBy similarity

GO - Molecular functioni

GO - Biological processi

  • ionotropic glutamate receptor signaling pathway Source: MGI
  • long-term memory Source: UniProtKB
  • long term synaptic depression Source: MGI
  • receptor internalization Source: UniProtKB
  • synaptic transmission Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-399710. Activation of AMPA receptors.
R-MMU-399719. Trafficking of AMPA receptors.
R-MMU-416993. Trafficking of GluR2-containing AMPA receptors.
R-MMU-438066. Unblocking of NMDA receptor, glutamate binding and activation.
R-MMU-5694530. Cargo concentration in the ER.
R-MMU-8849932. SALM protein interactions at the synapse.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor 1
Short name:
GluR-1
Alternative name(s):
AMPA-selective glutamate receptor 1
GluR-A
GluR-K1
Glutamate receptor ionotropic, AMPA 1
Short name:
GluA1
Gene namesi
Name:Gria1
Synonyms:Glur1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:95808. Gria1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 536518ExtracellularBy similarityAdd
BLAST
Transmembranei537 – 55721HelicalBy similarityAdd
BLAST
Topological domaini558 – 58427CytoplasmicBy similarityAdd
BLAST
Intramembranei585 – 60016Helical; Pore-formingBy similarityAdd
BLAST
Intramembranei601 – 6033By similarity
Topological domaini604 – 6096CytoplasmicBy similarity
Transmembranei610 – 63021HelicalBy similarityAdd
BLAST
Topological domaini631 – 805175ExtracellularBy similarityAdd
BLAST
Transmembranei806 – 82621Helical; Name=M4By similarityAdd
BLAST
Topological domaini827 – 90781CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • AMPA glutamate receptor complex Source: MGI
  • axonal spine Source: MGI
  • cell junction Source: UniProtKB-KW
  • cell surface Source: BHF-UCL
  • dendrite Source: UniProtKB
  • dendrite membrane Source: BHF-UCL
  • dendritic spine Source: UniProtKB
  • dendritic spine membrane Source: MGI
  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • ionotropic glutamate receptor complex Source: BHF-UCL
  • membrane Source: MGI
  • neuronal cell body Source: BHF-UCL
  • neuron projection Source: BHF-UCL
  • neuron spine Source: BHF-UCL
  • plasma membrane Source: BHF-UCL
  • postsynaptic density Source: UniProtKB
  • postsynaptic membrane Source: BHF-UCL
  • recycling endosome Source: MGI
  • synapse Source: MGI
  • synaptic vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Endoplasmic reticulum, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi905 – 9073TGL → AGA: Almost abolishes interaction with SHANK3. 1 Publication

Chemistry

ChEMBLiCHEMBL2096617.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 907889Glutamate receptor 1PRO_0000011530Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence analysis
Disulfide bondi75 ↔ 323By similarity
Glycosylationi249 – 2491N-linked (GlcNAc...)Sequence analysis
Glycosylationi257 – 2571N-linked (GlcNAc...)Sequence analysis
Glycosylationi363 – 3631N-linked (GlcNAc...)Sequence analysis
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence analysis
Glycosylationi406 – 4061N-linked (GlcNAc...)Sequence analysis
Lipidationi603 – 6031S-palmitoyl cysteine1 Publication
Modified residuei645 – 6451PhosphoserineBy similarity
Modified residuei710 – 7101PhosphoserineBy similarity
Disulfide bondi732 ↔ 787By similarity
Lipidationi829 – 8291S-palmitoyl cysteine1 Publication
Modified residuei849 – 8491PhosphoserineBy similarity
Modified residuei863 – 8631PhosphoserineBy similarity

Post-translational modificationi

Palmitoylated. Depalmitoylated upon glutamate stimulation. ZDHHC3/GODZ specifically palmitoylates Cys-603, which leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP23818.
PaxDbiP23818.
PRIDEiP23818.

PTM databases

iPTMnetiP23818.
PhosphoSiteiP23818.
SwissPalmiP23818.

Expressioni

Gene expression databases

BgeeiP23818.
CleanExiMM_GRIA1.
ExpressionAtlasiP23818. baseline and differential.
GenevisibleiP23818. MM.

Interactioni

Subunit structurei

Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2. Interacts with HIP1 and RASGRF2. Interacts with SYNDIG1 and GRIA2. Interacts with LRFN1. Found in a complex with GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with CNIH2, CACNG2 AND CACGN5. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes. Interacts (via PDZ-binding motif) with SHANK3 (via PDZ domain).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gria2P238195EBI-445486,EBI-77538

Protein-protein interaction databases

BioGridi200058. 9 interactions.
DIPiDIP-31970N.
IntActiP23818. 6 interactions.
MINTiMINT-1897734.
STRINGi10090.ENSMUSP00000044494.

Chemistry

BindingDBiP23818.

Structurei

3D structure databases

ProteinModelPortaliP23818.
SMRiP23818. Positions 22-831.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni492 – 4943Glutamate bindingBy similarity
Regioni668 – 6692Glutamate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi904 – 9074PDZ-binding

Domaini

The M4 transmembrane segment mediates tetramerization and is required for cell surface expression.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1054. Eukaryota.
ENOG410XPSH. LUCA.
GeneTreeiENSGT00760000118920.
HOGENOMiHOG000234372.
HOVERGENiHBG051839.
InParanoidiP23818.
KOiK05197.
OMAiAILGQXG.
OrthoDBiEOG7C2R0J.
TreeFamiTF315232.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYIFAFFCT GFLGAVVGAN FPNNIQIGGL FPNQQSQEHA AFRFALSQLT
60 70 80 90 100
EPPKLLPQID IVNISDSFEM TYRFCSQFSK GVYAIFGFYE RRTVNMLTSF
110 120 130 140 150
CGALHVCFIT PSFPVDTSNQ FVLQLRPELQ EALISIIDHY KWQTFVYIYD
160 170 180 190 200
ADRGLSVLQR VLDTAAEKNW QVTAVNILTT TEEGYRMLFQ DLEKKKERLV
210 220 230 240 250
VVDCESERLN AILGQIVKLE KNGIGYHYIL ANLGFMDIDL NKFKESGANV
260 270 280 290 300
TGFQLVNYTD TIPARIMQQW RTSDARDHTR VDWKRPKYTS ALTYDGVKVM
310 320 330 340 350
AEAFQSLRRQ RIDISRRGNA GDCLANPAVP WGQGIDIQRA LQQVRFEGLT
360 370 380 390 400
GNVQFNEKGR RTNYTLHVIE MKHDGIRKIG YWNEDDKFVP AATDAQAGGD
410 420 430 440 450
NSSVQNRTYI VTTILEDPYV MLKKNANQFE GNDRYEGYCV ELAAEIAKHV
460 470 480 490 500
GYSYRLEIVS DGKYGARDPD TKAWNGMVGE LVYGRADVAV APLTITLVRE
510 520 530 540 550
EVIDFSKPFM SLGISIMIKK PQKSKPGVFS FLDPLAYEIW MCIVFAYIGV
560 570 580 590 600
SVVLFLVSRF SPYEWHSEEF EEGRDQTTSD QSNEFGIFNS LWFSLGAFMQ
610 620 630 640 650
QGCDISPRSL SGRIVGGVWW FFTLIIISSY TANLAAFLTV ERMVSPIESA
660 670 680 690 700
EDLAKQTEIA YGTLEAGSTK EFFRRSKIAV FEKMWTYMKS AEPSVFVRTT
710 720 730 740 750
EEGMIRVRKS KGKYAYLLES TMNEYIEQRK PCDTMKVGGN LDSKGYGIAT
760 770 780 790 800
PKGSALRGPV NLAVLKLSEQ GVLDKLKSKW WYDKGECGSK DSGSKDKTSA
810 820 830 840 850
LSLSNVAGVF YILIGGLGLA MLVALIEFCY KSRSESKRMK GFCLIPQQSI
860 870 880 890 900
NEAIRTSTLP RNSGAGASGG SGSGENGRVV SQDFPKSMQS IPCMSHSSGM

PLGATGL
Length:907
Mass (Da):101,569
Last modified:November 1, 1991 - v1
Checksum:iF0FF7031DADD7CEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57497 mRNA. Translation: CAA40734.1.
CCDSiCCDS48800.1.
PIRiS12874.
RefSeqiNP_001106796.1. NM_001113325.2.
UniGeneiMm.4920.

Genome annotation databases

EnsembliENSMUST00000036315; ENSMUSP00000044494; ENSMUSG00000020524.
GeneIDi14799.
KEGGimmu:14799.
UCSCiuc007izs.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57497 mRNA. Translation: CAA40734.1.
CCDSiCCDS48800.1.
PIRiS12874.
RefSeqiNP_001106796.1. NM_001113325.2.
UniGeneiMm.4920.

3D structure databases

ProteinModelPortaliP23818.
SMRiP23818. Positions 22-831.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200058. 9 interactions.
DIPiDIP-31970N.
IntActiP23818. 6 interactions.
MINTiMINT-1897734.
STRINGi10090.ENSMUSP00000044494.

Chemistry

BindingDBiP23818.
ChEMBLiCHEMBL2096617.

PTM databases

iPTMnetiP23818.
PhosphoSiteiP23818.
SwissPalmiP23818.

Proteomic databases

MaxQBiP23818.
PaxDbiP23818.
PRIDEiP23818.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036315; ENSMUSP00000044494; ENSMUSG00000020524.
GeneIDi14799.
KEGGimmu:14799.
UCSCiuc007izs.3. mouse.

Organism-specific databases

CTDi2890.
MGIiMGI:95808. Gria1.

Phylogenomic databases

eggNOGiKOG1054. Eukaryota.
ENOG410XPSH. LUCA.
GeneTreeiENSGT00760000118920.
HOGENOMiHOG000234372.
HOVERGENiHBG051839.
InParanoidiP23818.
KOiK05197.
OMAiAILGQXG.
OrthoDBiEOG7C2R0J.
TreeFamiTF315232.

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-399710. Activation of AMPA receptors.
R-MMU-399719. Trafficking of AMPA receptors.
R-MMU-416993. Trafficking of GluR2-containing AMPA receptors.
R-MMU-438066. Unblocking of NMDA receptor, glutamate binding and activation.
R-MMU-5694530. Cargo concentration in the ER.
R-MMU-8849932. SALM protein interactions at the synapse.

Miscellaneous databases

ChiTaRSiGria1. mouse.
PROiP23818.
SOURCEiSearch...

Gene expression databases

BgeeiP23818.
CleanExiMM_GRIA1.
ExpressionAtlasiP23818. baseline and differential.
GenevisibleiP23818. MM.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Functional expression from cloned cDNAs of glutamate receptor species responsive to kainate and quisqualate."
    Sakimura K., Bujo H., Kushiya E., Araki K., Yamazaki M., Yamazaki M., Meguro H., Warashina A., Numa S., Mishina M.
    FEBS Lett. 272:73-80(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: INTERACTION WITH HIP1.
  3. "Differential regulation of AMPA receptor subunit trafficking by palmitoylation of two distinct sites."
    Hayashi T., Rumbaugh G., Huganir R.L.
    Neuron 47:709-723(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-603 AND CYS-829.
  4. "Age-dependent participation of Ras-GRF proteins in coupling calcium-permeable AMPA glutamate receptors to Ras/Erk signaling in cortical neurons."
    Tian X., Feig L.A.
    J. Biol. Chem. 281:7578-7582(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RASGRF2.
  5. "Direct interaction of post-synaptic density-95/Dlg/ZO-1 domain-containing synaptic molecule Shank3 with GluR1 alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor."
    Uchino S., Wada H., Honda S., Nakamura Y., Ondo Y., Uchiyama T., Tsutsumi M., Suzuki E., Hirasawa T., Kohsaka S.
    J. Neurochem. 97:1203-1214(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SHANK3, MUTAGENESIS OF 905-THR--LEU-907.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  7. "SynDIG1: an activity-regulated, AMPA- receptor-interacting transmembrane protein that regulates excitatory synapse development."
    Kalashnikova E., Lorca R.A., Kaur I., Barisone G.A., Li B., Ishimaru T., Trimmer J.S., Mohapatra D.P., Diaz E.
    Neuron 65:80-93(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SYNDIG1 AND GRIA2.
  8. "Hippocampal AMPA receptor gating controlled by both TARP and cornichon proteins."
    Kato A.S., Gill M.B., Ho M.T., Yu H., Tu Y., Siuda E.R., Wang H., Qian Y.W., Nisenbaum E.S., Tomita S., Bredt D.S.
    Neuron 68:1082-1096(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "Loss of sorting nexin 27 contributes to excitatory synaptic dysfunction by modulating glutamate receptor recycling in Down's syndrome."
    Wang X., Zhao Y., Zhang X., Badie H., Zhou Y., Mu Y., Loo L.S., Cai L., Thompson R.C., Yang B., Chen Y., Johnson P.F., Wu C., Bu G., Mobley W.C., Zhang D., Gage F.H., Ranscht B.
    , Zhang Y.W., Lipton S.A., Hong W., Xu H.
    Nat. Med. 19:473-480(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SNX27.

Entry informationi

Entry nameiGRIA1_MOUSE
AccessioniPrimary (citable) accession number: P23818
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 8, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.