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Protein

Polycomb group RING finger protein 2

Gene

Pcgf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Is not functionally redundant with BMI1; unlike BMI1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex.5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri18 – 5740RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: UniProtKB-KW
  • zinc ion binding Source: InterPro

GO - Biological processi

  • anterior/posterior pattern specification Source: MGI
  • cellular response to hydrogen peroxide Source: MGI
  • embryonic skeletal system development Source: MGI
  • embryonic skeletal system morphogenesis Source: MGI
  • histone acetylation Source: MGI
  • in utero embryonic development Source: MGI
  • negative regulation of apoptotic signaling pathway Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb group RING finger protein 2
Alternative name(s):
DNA-binding protein Mel-18
Melanoma nuclear protein 18
RING finger protein 110
Zinc finger protein 144
Short name:
Zfp-144
Gene namesi
Name:Pcgf2
Synonyms:Mel-18, Mel18, Rnf110, Zfp144, Znf144
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:99161. Pcgf2.

Subcellular locationi

GO - Cellular componenti

  • nuclear body Source: MGI
  • nuclear chromatin Source: MGI
  • nucleoplasm Source: Reactome
  • nucleus Source: MGI
  • PcG protein complex Source: MGI
  • PRC1 complex Source: UniProtKB
  • sex chromatin Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Probably related to tumorigenesis since it is expressed strongly in most tumor cell lines.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Polycomb group RING finger protein 2PRO_0000055985Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei237 – 2371Phosphothreonine; by PKASequence analysis
Modified residuei334 – 3341Phosphothreonine; by PKASequence analysis

Post-translational modificationi

Phosphorylated. Homodimer formation is regulated by phosphorylation with only unphosphorylated proteins forming homodimers.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23798.
PaxDbiP23798.
PeptideAtlasiP23798.
PRIDEiP23798.

PTM databases

iPTMnetiP23798.
PhosphoSiteiP23798.

Expressioni

Tissue specificityi

Expressed in a variety of tumor cells and in neural tissues.

Developmental stagei

Developmentally regulated.

Gene expression databases

BgeeiENSMUSG00000018537.
CleanExiMM_PCGF2.
ExpressionAtlasiP23798. baseline and differential.
GenevisibleiP23798. MM.

Interactioni

Subunit structurei

Exists as both a monomer and homodimer (By similarity). Component of a PRC1-like complex. Interacts WITH CBX8, RING1 AND RNF2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Phc1Q640284EBI-926857,EBI-927346
Phc2Q9QWH15EBI-926857,EBI-642357
Rnf2Q9CQJ415EBI-926857,EBI-927321

Protein-protein interaction databases

BioGridi204639. 12 interactions.
DIPiDIP-37645N.
IntActiP23798. 12 interactions.
MINTiMINT-1172293.
STRINGi10090.ENSMUSP00000018681.

Structurei

3D structure databases

ProteinModelPortaliP23798.
SMRiP23798. Positions 3-102.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi81 – 9515Nuclear localization signalSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi242 – 342101Pro/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri18 – 5740RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2660. Eukaryota.
ENOG410XPCN. LUCA.
GeneTreeiENSGT00550000074463.
HOGENOMiHOG000231945.
HOVERGENiHBG052826.
InParanoidiP23798.
KOiK11460.
OMAiDRDEKKG.
OrthoDBiEOG091G09IB.
PhylomeDBiP23798.
TreeFamiTF324206.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF16207. RAWUL. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRTTRIKIT ELNPHLMCAL CGGYFIDATT IVECLHSFCK TCIVRYLETN
60 70 80 90 100
KYCPMCDVQV HKTRPLLSIR SDKTLQDIVY KLVPGLFKDE MKRRRDFYAA
110 120 130 140 150
YPLTEVPNGS NEDRGEVLEQ EKGALGDDEI VSLSIEFYEG VRDREEKKNL
160 170 180 190 200
TENGDGDKEK TGVRFLRCPA AMTVMHLAKF LRNKMDVPSK YKVEILYEDE
210 220 230 240 250
PLKEYYTLMD IAYIYPWRRN GPLPLKYRVQ PACKRLTLPT VPTPSEGTNT
260 270 280 290 300
SGASECESVS DKAPSPATLP ATSSSLPSPA TPSHGSPSSH GPPATHPTSP
310 320 330 340
TPPSTAAGTT TATNGGTSNC LQTPSSTSRG RKMTVNGAPC PP
Length:342
Mass (Da):37,723
Last modified:May 1, 1992 - v2
Checksum:iC23031B8B9E30108
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90085 mRNA. Translation: BAA14122.1.
AF483502 mRNA. Translation: AAL90776.1.
AF483503 mRNA. Translation: AAL90777.1.
BC016419 mRNA. Translation: AAH16419.1.
CCDSiCCDS25327.1.
PIRiA37991.
RefSeqiNP_001156779.1. NM_001163307.1.
NP_001156780.1. NM_001163308.1.
NP_033571.1. NM_009545.2.
XP_011247274.1. XM_011248972.1.
XP_011247275.1. XM_011248973.1.
XP_011247276.1. XM_011248974.1.
XP_011247277.1. XM_011248975.1.
UniGeneiMm.2418.

Genome annotation databases

EnsembliENSMUST00000018681; ENSMUSP00000018681; ENSMUSG00000018537.
ENSMUST00000103148; ENSMUSP00000099437; ENSMUSG00000018537.
ENSMUST00000169807; ENSMUSP00000126967; ENSMUSG00000018537.
ENSMUST00000179765; ENSMUSP00000137517; ENSMUSG00000018537.
GeneIDi22658.
KEGGimmu:22658.
UCSCiuc007lel.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90085 mRNA. Translation: BAA14122.1.
AF483502 mRNA. Translation: AAL90776.1.
AF483503 mRNA. Translation: AAL90777.1.
BC016419 mRNA. Translation: AAH16419.1.
CCDSiCCDS25327.1.
PIRiA37991.
RefSeqiNP_001156779.1. NM_001163307.1.
NP_001156780.1. NM_001163308.1.
NP_033571.1. NM_009545.2.
XP_011247274.1. XM_011248972.1.
XP_011247275.1. XM_011248973.1.
XP_011247276.1. XM_011248974.1.
XP_011247277.1. XM_011248975.1.
UniGeneiMm.2418.

3D structure databases

ProteinModelPortaliP23798.
SMRiP23798. Positions 3-102.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204639. 12 interactions.
DIPiDIP-37645N.
IntActiP23798. 12 interactions.
MINTiMINT-1172293.
STRINGi10090.ENSMUSP00000018681.

PTM databases

iPTMnetiP23798.
PhosphoSiteiP23798.

Proteomic databases

MaxQBiP23798.
PaxDbiP23798.
PeptideAtlasiP23798.
PRIDEiP23798.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018681; ENSMUSP00000018681; ENSMUSG00000018537.
ENSMUST00000103148; ENSMUSP00000099437; ENSMUSG00000018537.
ENSMUST00000169807; ENSMUSP00000126967; ENSMUSG00000018537.
ENSMUST00000179765; ENSMUSP00000137517; ENSMUSG00000018537.
GeneIDi22658.
KEGGimmu:22658.
UCSCiuc007lel.2. mouse.

Organism-specific databases

CTDi7703.
MGIiMGI:99161. Pcgf2.

Phylogenomic databases

eggNOGiKOG2660. Eukaryota.
ENOG410XPCN. LUCA.
GeneTreeiENSGT00550000074463.
HOGENOMiHOG000231945.
HOVERGENiHBG052826.
InParanoidiP23798.
KOiK11460.
OMAiDRDEKKG.
OrthoDBiEOG091G09IB.
PhylomeDBiP23798.
TreeFamiTF324206.

Enzyme and pathway databases

ReactomeiR-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.

Miscellaneous databases

ChiTaRSiPcgf2. mouse.
PROiP23798.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018537.
CleanExiMM_PCGF2.
ExpressionAtlasiP23798. baseline and differential.
GenevisibleiP23798. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF16207. RAWUL. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCGF2_MOUSE
AccessioniPrimary (citable) accession number: P23798
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: May 1, 1992
Last modified: September 7, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.