Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase

Gene

GPI12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the second step of GPI biosynthesis. De-N-acetylation of N-acetylglucosaminyl-phosphatidylinositol.

Catalytic activityi

6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate.

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

  • N-acetylglucosaminylphosphatidylinositol deacetylase activity Source: SGD

GO - Biological processi

  • GPI anchor biosynthetic process Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processGPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YMR281W-MONOMER
ReactomeiR-SCE-162710 Synthesis of glycosylphosphatidylinositol (GPI)
UniPathwayiUPA00196

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (EC:3.5.1.89)
Gene namesi
Name:GPI12
Ordered Locus Names:YMR281W
ORF Names:YM8021.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR281W
SGDiS000004894 GPI12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20LumenalSequence analysisAdd BLAST20
Transmembranei21 – 38HelicalSequence analysisAdd BLAST18
Topological domaini39 – 304CytoplasmicSequence analysisAdd BLAST266

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002071701 – 304N-acetylglucosaminyl-phosphatidylinositol de-N-acetylaseAdd BLAST304

Proteomic databases

MaxQBiP23797
PaxDbiP23797
PRIDEiP23797

PTM databases

iPTMnetiP23797

Interactioni

Protein-protein interaction databases

BioGridi35460, 369 interactors
IntActiP23797, 1 interactor
STRINGi4932.YMR281W

Structurei

3D structure databases

ProteinModelPortaliP23797
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGL family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000018434
HOGENOMiHOG000176611
InParanoidiP23797
KOiK03434
OMAiLVWFRWL
OrthoDBiEOG092C3E4U

Family and domain databases

Gene3Di3.40.50.10320, 1 hit
InterProiView protein in InterPro
IPR003737 GlcNAc_PI_deacetylase-related
IPR024078 LmbE-like_dom_sf
PANTHERiPTHR12993 PTHR12993, 1 hit
PfamiView protein in Pfam
PF02585 PIG-L, 1 hit
SUPFAMiSSF102588 SSF102588, 1 hit

Sequencei

Sequence statusi: Complete.

P23797-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMLRRTKVN FSKLLYKITK LAIVLTILYI YFTPKIVSRN NASLQHIFPH
60 70 80 90 100
KYGDYEINLV IAHPDDEVMF FSPIISQLNS YFPRTVPFNI ICLSKGNAEG
110 120 130 140 150
LGETRVRELN ESAALLLHNE RAVSVQVMDF QDGMDEIWDI DSITSSLSQK
160 170 180 190 200
IDIKNHNLNQ IIVTFDSYGV SNHINHKSCY AAVKKLVDDY AQPKTKRNEQ
210 220 230 240 250
PPHVTALYLR SYKNNIVLKY NSFIWEILKI LYDLISPFRR IIQALPPNTA
260 270 280 290 300
AEKDKLSLMN THAQYVLAFA TMLNAHESQV VWFRYGWWIF SRFVFVNEFD

VYTY
Length:304
Mass (Da):35,446
Last modified:October 1, 1996 - v2
Checksum:i7A3B7A16D7B72DD7
GO

Sequence cautioni

The sequence M59860 differs from that shown. Reason: Frameshift at position 64.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017166 Genomic DNA Translation: BAA74776.1
M59860 Genomic DNA No translation available.
Z49704 Genomic DNA Translation: CAA89779.1
BK006946 Genomic DNA Translation: DAA10182.1
PIRiS54588
RefSeqiNP_014008.1, NM_001182788.1

Genome annotation databases

EnsemblFungiiYMR281W; YMR281W; YMR281W
GeneIDi855324
KEGGisce:YMR281W

Similar proteinsi

Entry informationi

Entry nameiGPI12_YEAST
AccessioniPrimary (citable) accession number: P23797
Secondary accession number(s): D6W0A8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health