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P23797

- GPI12_YEAST

UniProt

P23797 - GPI12_YEAST

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Protein

N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase

Gene

GPI12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the second step of GPI biosynthesis. De-N-acetylation of N-acetylglucosaminyl-phosphatidylinositol.

Catalytic activityi

6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate.

Pathwayi

GO - Molecular functioni

  1. N-acetylglucosaminylphosphatidylinositol deacetylase activity Source: SGD

GO - Biological processi

  1. GPI anchor biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YMR281W-MONOMER.
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (EC:3.5.1.89)
Gene namesi
Name:GPI12
Ordered Locus Names:YMR281W
ORF Names:YM8021.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR281w.
SGDiS000004894. GPI12.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304N-acetylglucosaminyl-phosphatidylinositol de-N-acetylasePRO_0000207170Add
BLAST

Proteomic databases

MaxQBiP23797.
PaxDbiP23797.

Expressioni

Gene expression databases

GenevestigatoriP23797.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TAF2P232551EBI-7773,EBI-18862

Protein-protein interaction databases

BioGridi35460. 8 interactions.
IntActiP23797. 1 interaction.
MINTiMINT-4498932.
STRINGi4932.YMR281W.

Structurei

3D structure databases

ProteinModelPortaliP23797.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020LumenalSequence AnalysisAdd
BLAST
Topological domaini39 – 304266CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei21 – 3818HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGL family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2120.
GeneTreeiENSGT00390000018434.
HOGENOMiHOG000176611.
InParanoidiP23797.
KOiK03434.
OMAiWSRERRI.
OrthoDBiEOG7DFXPJ.

Family and domain databases

Gene3Di3.40.50.10320. 1 hit.
InterProiIPR003737. GlcNAc_PI_deacetylase-related.
IPR024078. LmbE-like_dom.
[Graphical view]
PANTHERiPTHR12993. PTHR12993. 1 hit.
PfamiPF02585. PIG-L. 1 hit.
[Graphical view]
SUPFAMiSSF102588. SSF102588. 1 hit.

Sequencei

Sequence statusi: Complete.

P23797-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKMLRRTKVN FSKLLYKITK LAIVLTILYI YFTPKIVSRN NASLQHIFPH
60 70 80 90 100
KYGDYEINLV IAHPDDEVMF FSPIISQLNS YFPRTVPFNI ICLSKGNAEG
110 120 130 140 150
LGETRVRELN ESAALLLHNE RAVSVQVMDF QDGMDEIWDI DSITSSLSQK
160 170 180 190 200
IDIKNHNLNQ IIVTFDSYGV SNHINHKSCY AAVKKLVDDY AQPKTKRNEQ
210 220 230 240 250
PPHVTALYLR SYKNNIVLKY NSFIWEILKI LYDLISPFRR IIQALPPNTA
260 270 280 290 300
AEKDKLSLMN THAQYVLAFA TMLNAHESQV VWFRYGWWIF SRFVFVNEFD

VYTY
Length:304
Mass (Da):35,446
Last modified:October 1, 1996 - v2
Checksum:i7A3B7A16D7B72DD7
GO

Sequence cautioni

The sequence M59860 differs from that shown. Reason: Frameshift at position 64.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB017166 Genomic DNA. Translation: BAA74776.1.
M59860 Genomic DNA. No translation available.
Z49704 Genomic DNA. Translation: CAA89779.1.
BK006946 Genomic DNA. Translation: DAA10182.1.
PIRiS54588.
RefSeqiNP_014008.1. NM_001182788.1.

Genome annotation databases

EnsemblFungiiYMR281W; YMR281W; YMR281W.
GeneIDi855324.
KEGGisce:YMR281W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB017166 Genomic DNA. Translation: BAA74776.1 .
M59860 Genomic DNA. No translation available.
Z49704 Genomic DNA. Translation: CAA89779.1 .
BK006946 Genomic DNA. Translation: DAA10182.1 .
PIRi S54588.
RefSeqi NP_014008.1. NM_001182788.1.

3D structure databases

ProteinModelPortali P23797.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35460. 8 interactions.
IntActi P23797. 1 interaction.
MINTi MINT-4498932.
STRINGi 4932.YMR281W.

Proteomic databases

MaxQBi P23797.
PaxDbi P23797.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR281W ; YMR281W ; YMR281W .
GeneIDi 855324.
KEGGi sce:YMR281W.

Organism-specific databases

CYGDi YMR281w.
SGDi S000004894. GPI12.

Phylogenomic databases

eggNOGi COG2120.
GeneTreei ENSGT00390000018434.
HOGENOMi HOG000176611.
InParanoidi P23797.
KOi K03434.
OMAi WSRERRI.
OrthoDBi EOG7DFXPJ.

Enzyme and pathway databases

UniPathwayi UPA00196 .
BioCyci YEAST:YMR281W-MONOMER.

Miscellaneous databases

NextBioi 979035.
PROi P23797.

Gene expression databases

Genevestigatori P23797.

Family and domain databases

Gene3Di 3.40.50.10320. 1 hit.
InterProi IPR003737. GlcNAc_PI_deacetylase-related.
IPR024078. LmbE-like_dom.
[Graphical view ]
PANTHERi PTHR12993. PTHR12993. 1 hit.
Pfami PF02585. PIG-L. 1 hit.
[Graphical view ]
SUPFAMi SSF102588. SSF102588. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis."
    Watanabe R., Ohishi K., Maeda Y., Nakamura N., Kinoshita T.
    Biochem. J. 339:185-192(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. "Characterization of a yeast nuclear gene, AEP2, required for accumulation of mitochondrial mRNA encoding subunit 9 of the ATP synthase."
    Payne M.J., Finnegan P.M., Keramidaris E., Lukins H.B.
    Curr. Genet. 20:53-61(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiGPI12_YEAST
AccessioniPrimary (citable) accession number: P23797
Secondary accession number(s): D6W0A8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3