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P23797

- GPI12_YEAST

UniProt

P23797 - GPI12_YEAST

Protein

N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase

Gene

GPI12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in the second step of GPI biosynthesis. De-N-acetylation of N-acetylglucosaminyl-phosphatidylinositol.

    Catalytic activityi

    6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate.

    Pathwayi

    GO - Molecular functioni

    1. N-acetylglucosaminylphosphatidylinositol deacetylase activity Source: SGD

    GO - Biological processi

    1. GPI anchor biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    GPI-anchor biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YMR281W-MONOMER.
    UniPathwayiUPA00196.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (EC:3.5.1.89)
    Gene namesi
    Name:GPI12
    Ordered Locus Names:YMR281W
    ORF Names:YM8021.07
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR281w.
    SGDiS000004894. GPI12.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: SGD
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 304304N-acetylglucosaminyl-phosphatidylinositol de-N-acetylasePRO_0000207170Add
    BLAST

    Proteomic databases

    MaxQBiP23797.
    PaxDbiP23797.

    Expressioni

    Gene expression databases

    GenevestigatoriP23797.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TAF2P232551EBI-7773,EBI-18862

    Protein-protein interaction databases

    BioGridi35460. 8 interactions.
    IntActiP23797. 1 interaction.
    MINTiMINT-4498932.
    STRINGi4932.YMR281W.

    Structurei

    3D structure databases

    ProteinModelPortaliP23797.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2020LumenalSequence AnalysisAdd
    BLAST
    Topological domaini39 – 304266CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 3818HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PIGL family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2120.
    GeneTreeiENSGT00390000018434.
    HOGENOMiHOG000176611.
    KOiK03434.
    OMAiWSRERRI.
    OrthoDBiEOG7DFXPJ.

    Family and domain databases

    Gene3Di3.40.50.10320. 1 hit.
    InterProiIPR003737. GlcNAc_PI_deacetylase-related.
    IPR024078. LmbE-like_dom.
    [Graphical view]
    PANTHERiPTHR12993. PTHR12993. 1 hit.
    PfamiPF02585. PIG-L. 1 hit.
    [Graphical view]
    SUPFAMiSSF102588. SSF102588. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P23797-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKMLRRTKVN FSKLLYKITK LAIVLTILYI YFTPKIVSRN NASLQHIFPH    50
    KYGDYEINLV IAHPDDEVMF FSPIISQLNS YFPRTVPFNI ICLSKGNAEG 100
    LGETRVRELN ESAALLLHNE RAVSVQVMDF QDGMDEIWDI DSITSSLSQK 150
    IDIKNHNLNQ IIVTFDSYGV SNHINHKSCY AAVKKLVDDY AQPKTKRNEQ 200
    PPHVTALYLR SYKNNIVLKY NSFIWEILKI LYDLISPFRR IIQALPPNTA 250
    AEKDKLSLMN THAQYVLAFA TMLNAHESQV VWFRYGWWIF SRFVFVNEFD 300
    VYTY 304
    Length:304
    Mass (Da):35,446
    Last modified:October 1, 1996 - v2
    Checksum:i7A3B7A16D7B72DD7
    GO

    Sequence cautioni

    The sequence M59860 differs from that shown. Reason: Frameshift at position 64.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017166 Genomic DNA. Translation: BAA74776.1.
    M59860 Genomic DNA. No translation available.
    Z49704 Genomic DNA. Translation: CAA89779.1.
    BK006946 Genomic DNA. Translation: DAA10182.1.
    PIRiS54588.
    RefSeqiNP_014008.1. NM_001182788.1.

    Genome annotation databases

    EnsemblFungiiYMR281W; YMR281W; YMR281W.
    GeneIDi855324.
    KEGGisce:YMR281W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB017166 Genomic DNA. Translation: BAA74776.1 .
    M59860 Genomic DNA. No translation available.
    Z49704 Genomic DNA. Translation: CAA89779.1 .
    BK006946 Genomic DNA. Translation: DAA10182.1 .
    PIRi S54588.
    RefSeqi NP_014008.1. NM_001182788.1.

    3D structure databases

    ProteinModelPortali P23797.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35460. 8 interactions.
    IntActi P23797. 1 interaction.
    MINTi MINT-4498932.
    STRINGi 4932.YMR281W.

    Proteomic databases

    MaxQBi P23797.
    PaxDbi P23797.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR281W ; YMR281W ; YMR281W .
    GeneIDi 855324.
    KEGGi sce:YMR281W.

    Organism-specific databases

    CYGDi YMR281w.
    SGDi S000004894. GPI12.

    Phylogenomic databases

    eggNOGi COG2120.
    GeneTreei ENSGT00390000018434.
    HOGENOMi HOG000176611.
    KOi K03434.
    OMAi WSRERRI.
    OrthoDBi EOG7DFXPJ.

    Enzyme and pathway databases

    UniPathwayi UPA00196 .
    BioCyci YEAST:YMR281W-MONOMER.

    Miscellaneous databases

    NextBioi 979035.
    PROi P23797.

    Gene expression databases

    Genevestigatori P23797.

    Family and domain databases

    Gene3Di 3.40.50.10320. 1 hit.
    InterProi IPR003737. GlcNAc_PI_deacetylase-related.
    IPR024078. LmbE-like_dom.
    [Graphical view ]
    PANTHERi PTHR12993. PTHR12993. 1 hit.
    Pfami PF02585. PIG-L. 1 hit.
    [Graphical view ]
    SUPFAMi SSF102588. SSF102588. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis."
      Watanabe R., Ohishi K., Maeda Y., Nakamura N., Kinoshita T.
      Biochem. J. 339:185-192(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    2. "Characterization of a yeast nuclear gene, AEP2, required for accumulation of mitochondrial mRNA encoding subunit 9 of the ATP synthase."
      Payne M.J., Finnegan P.M., Keramidaris E., Lukins H.B.
      Curr. Genet. 20:53-61(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.

    Entry informationi

    Entry nameiGPI12_YEAST
    AccessioniPrimary (citable) accession number: P23797
    Secondary accession number(s): D6W0A8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3