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P23797 (GPI12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase

EC=3.5.1.89
Gene names
Name:GPI12
Ordered Locus Names:YMR281W
ORF Names:YM8021.07
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the second step of GPI biosynthesis. De-N-acetylation of N-acetylglucosaminyl-phosphatidylinositol.

Catalytic activity

6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate.

Pathway

Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.

Subcellular location

Endoplasmic reticulum membrane.

Sequence similarities

Belongs to the PIGL family.

Sequence caution

The sequence M59860 differs from that shown. Reason: Frameshift at position 64.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TAF2P232551EBI-7773,EBI-18862

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 304304N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase
PRO_0000207170

Regions

Topological domain1 – 2020Lumenal Potential
Transmembrane21 – 3818Helical; Potential
Topological domain39 – 304266Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P23797 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 7A3B7A16D7B72DD7

FASTA30435,446
        10         20         30         40         50         60 
MKMLRRTKVN FSKLLYKITK LAIVLTILYI YFTPKIVSRN NASLQHIFPH KYGDYEINLV 

        70         80         90        100        110        120 
IAHPDDEVMF FSPIISQLNS YFPRTVPFNI ICLSKGNAEG LGETRVRELN ESAALLLHNE 

       130        140        150        160        170        180 
RAVSVQVMDF QDGMDEIWDI DSITSSLSQK IDIKNHNLNQ IIVTFDSYGV SNHINHKSCY 

       190        200        210        220        230        240 
AAVKKLVDDY AQPKTKRNEQ PPHVTALYLR SYKNNIVLKY NSFIWEILKI LYDLISPFRR 

       250        260        270        280        290        300 
IIQALPPNTA AEKDKLSLMN THAQYVLAFA TMLNAHESQV VWFRYGWWIF SRFVFVNEFD 


VYTY 

« Hide

References

« Hide 'large scale' references
[1]"Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis."
Watanabe R., Ohishi K., Maeda Y., Nakamura N., Kinoshita T.
Biochem. J. 339:185-192(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]"Characterization of a yeast nuclear gene, AEP2, required for accumulation of mitochondrial mRNA encoding subunit 9 of the ATP synthase."
Payne M.J., Finnegan P.M., Keramidaris E., Lukins H.B.
Curr. Genet. 20:53-61(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB017166 Genomic DNA. Translation: BAA74776.1.
M59860 Genomic DNA. No translation available.
Z49704 Genomic DNA. Translation: CAA89779.1.
BK006946 Genomic DNA. Translation: DAA10182.1.
PIRS54588.
RefSeqNP_014008.1. NM_001182788.1.

3D structure databases

ProteinModelPortalP23797.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35460. 8 interactions.
IntActP23797. 1 interaction.
MINTMINT-4498932.
STRING4932.YMR281W.

Proteomic databases

MaxQBP23797.
PaxDbP23797.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR281W; YMR281W; YMR281W.
GeneID855324.
KEGGsce:YMR281W.

Organism-specific databases

CYGDYMR281w.
SGDS000004894. GPI12.

Phylogenomic databases

eggNOGCOG2120.
GeneTreeENSGT00390000018434.
HOGENOMHOG000176611.
KOK03434.
OMAWSRERRI.
OrthoDBEOG7DFXPJ.

Enzyme and pathway databases

BioCycYEAST:YMR281W-MONOMER.
UniPathwayUPA00196.

Gene expression databases

GenevestigatorP23797.

Family and domain databases

Gene3D3.40.50.10320. 1 hit.
InterProIPR003737. GlcNAc_PI_deacetylase-related.
IPR024078. LmbE-like_dom.
[Graphical view]
PANTHERPTHR12993. PTHR12993. 1 hit.
PfamPF02585. PIG-L. 1 hit.
[Graphical view]
SUPFAMSSF102588. SSF102588. 1 hit.
ProtoNetSearch...

Other

NextBio979035.
PROP23797.

Entry information

Entry nameGPI12_YEAST
AccessionPrimary (citable) accession number: P23797
Secondary accession number(s): D6W0A8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways