ID   RIT1_YEAST              Reviewed;         513 AA.
AC   P23796; D6W0B0;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 3.
DT   27-MAR-2024, entry version 160.
DE   RecName: Full=tRNA A64-2'-O-ribosylphosphate transferase;
DE            EC=2.4.2.-;
DE   AltName: Full=Initiator tRNA phosphoribosyl-transferase;
GN   Name=RIT1; OrderedLocusNames=YMR283C; ORFNames=YM8021.09C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-513.
RC   STRAIN=S288c / YPH1;
RX   PubMed=7954819; DOI=10.1016/0092-8674(94)90262-3;
RA   Aastroem S.U., Bystroem A.S.;
RT   "Rit1, a tRNA backbone-modifying enzyme that mediates initiator and
RT   elongator tRNA discrimination.";
RL   Cell 79:535-546(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 418-513.
RX   PubMed=1718609; DOI=10.1007/bf00312765;
RA   Payne M.J., Finnegan P.M., Keramidaris E., Lukins H.B.;
RT   "Characterization of a yeast nuclear gene, AEP2, required for accumulation
RT   of mitochondrial mRNA encoding subunit 9 of the ATP synthase.";
RL   Curr. Genet. 20:53-61(1991).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: tRNA backbone modifying enzyme that mediates initiator/
CC       elongator tRNA discrimination. This enzyme modifies exclusively the
CC       initiator tRNA in position 64 using 5'-phosphoribosyl-1'-pyrophosphate
CC       as the modification donor. Recognize the stem-loop IV region that is
CC       unique in eukaryotic cytoplasmic initiator tRNAs.
CC   -!- MISCELLANEOUS: Present with 259 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; X80795; CAA56770.1; -; Genomic_DNA.
DR   EMBL; Z49704; CAA89781.1; -; Genomic_DNA.
DR   EMBL; AY692815; AAT92834.1; -; Genomic_DNA.
DR   EMBL; M59860; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BK006946; DAA10184.1; -; Genomic_DNA.
DR   PIR; S54590; S54590.
DR   RefSeq; NP_014010.1; NM_001182790.1.
DR   AlphaFoldDB; P23796; -.
DR   SMR; P23796; -.
DR   BioGRID; 35462; 121.
DR   STRING; 4932.YMR283C; -.
DR   iPTMnet; P23796; -.
DR   MaxQB; P23796; -.
DR   PaxDb; 4932-YMR283C; -.
DR   PeptideAtlas; P23796; -.
DR   EnsemblFungi; YMR283C_mRNA; YMR283C; YMR283C.
DR   GeneID; 855326; -.
DR   KEGG; sce:YMR283C; -.
DR   AGR; SGD:S000004896; -.
DR   SGD; S000004896; RIT1.
DR   VEuPathDB; FungiDB:YMR283C; -.
DR   eggNOG; KOG2634; Eukaryota.
DR   HOGENOM; CLU_027654_1_1_1; -.
DR   InParanoid; P23796; -.
DR   OMA; HGGCVIV; -.
DR   OrthoDB; 7104at2759; -.
DR   BioCyc; YEAST:G3O-32953-MONOMER; -.
DR   BioGRID-ORCS; 855326; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P23796; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; P23796; Protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0016763; F:pentosyltransferase activity; IDA:SGD.
DR   GO; GO:0043399; F:tRNA A64-2'-O-ribosylphosphate transferase activity; IEA:InterPro.
DR   GO; GO:0019988; P:charged-tRNA amino acid modification; IMP:SGD.
DR   InterPro; IPR007306; Rit1.
DR   InterPro; IPR033421; Rit1_DUSP-like.
DR   InterPro; IPR033449; Rit1_N.
DR   PANTHER; PTHR31811; TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE; 1.
DR   PANTHER; PTHR31811:SF0; TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE; 1.
DR   Pfam; PF04179; Init_tRNA_PT; 1.
DR   Pfam; PF17184; Rit1_C; 1.
DR   PIRSF; PIRSF007747; Ribosyl_Ptfrase; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Reference proteome; Transferase; tRNA processing.
FT   CHAIN           1..513
FT                   /note="tRNA A64-2'-O-ribosylphosphate transferase"
FT                   /id="PRO_0000097353"
SQ   SEQUENCE   513 AA;  58882 MW;  36F97B2445A4EA09 CRC64;
     MVSRLSKRYR SRVQFSISVM DENIYLSLSQ INKDIRKENK SVRNRLQSIL LDNKFLQDRV
     IPIFPHYPLI PNERCGLWYC NPSSFKQTSY FKSTDGHVNQ WDFSTRRLNF HLLETIRDNK
     GIIIVDSTRR GKKIPDALSK TVPIWCAVLN TLMLQETEKN VAIDKVLYLP PETVPKSEYD
     MIKRKIPELV AKLQKLNIID SKKLNELFMG KLLRPIWVHP GSSLLDHSVD YFTGEVQEYE
     AWETPEDQNI IPIILCTVSY QAQDGMDKRY GFTYVQGAAD DHELWSFGLD SNMFWAHIEY
     LGDASYSDDQ LHDYIMDLAA AKLRNQCYIQ DKGSLDEVFG NIDKITNEIS LGKVSSGLTI
     NKNLKQKLKS EYGKVIIFSN SVTVAEDTDD EEESGTDPFI SIYKLQSGDK KSSKALRSTF
     PRIHGEIQSL FTNRDEKIKP MLICCNTGTD MSIGVILSIL CTKYTEEWML TSELPDISKL
     IVRKHLTKLI SHLKGRNVNP SRATLNSVNS FLM
//