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Protein

tRNA A64-2'-O-ribosylphosphate transferase

Gene

RIT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

tRNA backbone modifying enzyme that mediates initiator/ elongator tRNA discrimination. This enzyme modifies exclusively the initiator tRNA in position 64 using 5'-phosphoribosyl-1'-pyrophosphate as the modification donor. Recognize the stem-loop IV region that is unique in eukaryotic cytoplasmic initiator tRNAs.

GO - Molecular functioni

  1. transferase activity, transferring pentosyl groups Source: SGD

GO - Biological processi

  1. charged-tRNA amino acid modification Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-32953-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA A64-2'-O-ribosylphosphate transferase (EC:2.4.2.-)
Alternative name(s):
Initiator tRNA phosphoribosyl-transferase
Gene namesi
Name:RIT1
Ordered Locus Names:YMR283C
ORF Names:YM8021.09C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR283c.
SGDiS000004896. RIT1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 513513tRNA A64-2'-O-ribosylphosphate transferasePRO_0000097353Add
BLAST

Proteomic databases

MaxQBiP23796.
PaxDbiP23796.

Expressioni

Gene expression databases

GenevestigatoriP23796.

Interactioni

Protein-protein interaction databases

BioGridi35462. 51 interactions.
MINTiMINT-4498956.
STRINGi4932.YMR283C.

Structurei

3D structure databases

ProteinModelPortaliP23796.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG241557.
HOGENOMiHOG000174281.
KOiK15463.
OMAiCAVLNTL.
OrthoDBiEOG7B05P8.

Family and domain databases

InterProiIPR007306. Rit1.
[Graphical view]
PfamiPF04179. Init_tRNA_PT. 1 hit.
[Graphical view]
PIRSFiPIRSF007747. Ribosyl_Ptfrase. 1 hit.

Sequencei

Sequence statusi: Complete.

P23796-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSRLSKRYR SRVQFSISVM DENIYLSLSQ INKDIRKENK SVRNRLQSIL
60 70 80 90 100
LDNKFLQDRV IPIFPHYPLI PNERCGLWYC NPSSFKQTSY FKSTDGHVNQ
110 120 130 140 150
WDFSTRRLNF HLLETIRDNK GIIIVDSTRR GKKIPDALSK TVPIWCAVLN
160 170 180 190 200
TLMLQETEKN VAIDKVLYLP PETVPKSEYD MIKRKIPELV AKLQKLNIID
210 220 230 240 250
SKKLNELFMG KLLRPIWVHP GSSLLDHSVD YFTGEVQEYE AWETPEDQNI
260 270 280 290 300
IPIILCTVSY QAQDGMDKRY GFTYVQGAAD DHELWSFGLD SNMFWAHIEY
310 320 330 340 350
LGDASYSDDQ LHDYIMDLAA AKLRNQCYIQ DKGSLDEVFG NIDKITNEIS
360 370 380 390 400
LGKVSSGLTI NKNLKQKLKS EYGKVIIFSN SVTVAEDTDD EEESGTDPFI
410 420 430 440 450
SIYKLQSGDK KSSKALRSTF PRIHGEIQSL FTNRDEKIKP MLICCNTGTD
460 470 480 490 500
MSIGVILSIL CTKYTEEWML TSELPDISKL IVRKHLTKLI SHLKGRNVNP
510
SRATLNSVNS FLM
Length:513
Mass (Da):58,882
Last modified:January 31, 1996 - v3
Checksum:i36F97B2445A4EA09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80795 Genomic DNA. Translation: CAA56770.1.
Z49704 Genomic DNA. Translation: CAA89781.1.
AY692815 Genomic DNA. Translation: AAT92834.1.
M59860 Genomic DNA. No translation available.
BK006946 Genomic DNA. Translation: DAA10184.1.
PIRiS54590.
RefSeqiNP_014010.1. NM_001182790.1.

Genome annotation databases

EnsemblFungiiYMR283C; YMR283C; YMR283C.
GeneIDi855326.
KEGGisce:YMR283C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80795 Genomic DNA. Translation: CAA56770.1.
Z49704 Genomic DNA. Translation: CAA89781.1.
AY692815 Genomic DNA. Translation: AAT92834.1.
M59860 Genomic DNA. No translation available.
BK006946 Genomic DNA. Translation: DAA10184.1.
PIRiS54590.
RefSeqiNP_014010.1. NM_001182790.1.

3D structure databases

ProteinModelPortaliP23796.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35462. 51 interactions.
MINTiMINT-4498956.
STRINGi4932.YMR283C.

Proteomic databases

MaxQBiP23796.
PaxDbiP23796.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR283C; YMR283C; YMR283C.
GeneIDi855326.
KEGGisce:YMR283C.

Organism-specific databases

CYGDiYMR283c.
SGDiS000004896. RIT1.

Phylogenomic databases

eggNOGiNOG241557.
HOGENOMiHOG000174281.
KOiK15463.
OMAiCAVLNTL.
OrthoDBiEOG7B05P8.

Enzyme and pathway databases

BioCyciYEAST:G3O-32953-MONOMER.

Miscellaneous databases

NextBioi979041.

Gene expression databases

GenevestigatoriP23796.

Family and domain databases

InterProiIPR007306. Rit1.
[Graphical view]
PfamiPF04179. Init_tRNA_PT. 1 hit.
[Graphical view]
PIRSFiPIRSF007747. Ribosyl_Ptfrase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination."
    Aastroem S.U., Bystroem A.S.
    Cell 79:535-546(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-513.
    Strain: S288c / YPH1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Characterization of a yeast nuclear gene, AEP2, required for accumulation of mitochondrial mRNA encoding subunit 9 of the ATP synthase."
    Payne M.J., Finnegan P.M., Keramidaris E., Lukins H.B.
    Curr. Genet. 20:53-61(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 418-513.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRIT1_YEAST
AccessioniPrimary (citable) accession number: P23796
Secondary accession number(s): D6W0B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1991
Last sequence update: January 31, 1996
Last modified: March 31, 2015
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.