P23796 (RIT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA A64-2'-O-ribosylphosphate transferase
EC=2.4.2.-

Alternative name(s):
Initiator tRNA phosphoribosyl-transferase

Gene names

Name:	RIT1
Ordered Locus Names:	YMR283C
ORF Names:	YM8021.09C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	tRNA backbone modifying enzyme that mediates initiator/ elongator tRNA discrimination. This enzyme modifies exclusively the initiator tRNA in position 64 using 5'-phosphoribosyl-1'-pyrophosphate as the modification donor. Recognize the stem-loop IV region that is unique in eukaryotic cytoplasmic initiator tRNAs.
Miscellaneous	Present with 259 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	charged-tRNA amino acid modification Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	transferase activity, transferring pentosyl groups Inferred from genetic interaction Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	tRNA A64-2'-O-ribosylphosphate transferase		PRO_0000097353

Sequences

	Sequence		Length	Mass (Da)
	P23796 [UniParc]. Last modified February 1, 1996. Version 3. Checksum: 36F97B2445A4EA09	FASTA	513	58,882
	10 20 30 40 50 60 MVSRLSKRYR SRVQFSISVM DENIYLSLSQ INKDIRKENK SVRNRLQSIL LDNKFLQDRV 70 80 90 100 110 120 IPIFPHYPLI PNERCGLWYC NPSSFKQTSY FKSTDGHVNQ WDFSTRRLNF HLLETIRDNK 130 140 150 160 170 180 GIIIVDSTRR GKKIPDALSK TVPIWCAVLN TLMLQETEKN VAIDKVLYLP PETVPKSEYD 190 200 210 220 230 240 MIKRKIPELV AKLQKLNIID SKKLNELFMG KLLRPIWVHP GSSLLDHSVD YFTGEVQEYE 250 260 270 280 290 300 AWETPEDQNI IPIILCTVSY QAQDGMDKRY GFTYVQGAAD DHELWSFGLD SNMFWAHIEY 310 320 330 340 350 360 LGDASYSDDQ LHDYIMDLAA AKLRNQCYIQ DKGSLDEVFG NIDKITNEIS LGKVSSGLTI 370 380 390 400 410 420 NKNLKQKLKS EYGKVIIFSN SVTVAEDTDD EEESGTDPFI SIYKLQSGDK KSSKALRSTF 430 440 450 460 470 480 PRIHGEIQSL FTNRDEKIKP MLICCNTGTD MSIGVILSIL CTKYTEEWML TSELPDISKL 490 500 510 IVRKHLTKLI SHLKGRNVNP SRATLNSVNS FLM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination." Aastroem S.U., Bystroem A.S. Cell 79:535-546(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-513. Strain: S288c / YPH1.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Characterization of a yeast nuclear gene, AEP2, required for accumulation of mitochondrial mRNA encoding subunit 9 of the ATP synthase." Payne M.J., Finnegan P.M., Keramidaris E., Lukins H.B. Curr. Genet. 20:53-61(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 418-513.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80795 Genomic DNA. Translation: CAA56770.1. Z49704 Genomic DNA. Translation: CAA89781.1. AY692815 Genomic DNA. Translation: AAT92834.1. M59860 Genomic DNA. No translation available. BK006946 Genomic DNA. Translation: DAA10184.1.
PIR	S54590.
RefSeq	NP_014010.1. NM_001182790.1.
3D structure databases
ProteinModelPortal	P23796.
ModBase	Search...
Protein-protein interaction databases
STRING	4932.YMR283C.
Proteomic databases
PaxDb	P23796.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR283C; YMR283C; YMR283C.
GeneID	855326.
KEGG	sce:YMR283C.
Organism-specific databases
CYGD	YMR283c.
SGD	S000004896. RIT1.
Phylogenomic databases
eggNOG	NOG241557.
HOGENOM	HOG000174281.
KO	K15463.
OMA	ADDHELW.
OrthoDB	EOG49W5Q9.
Gene expression databases
Genevestigator	P23796.
GermOnline	YMR283C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007306. Init_tRNA_PT. IPR016519. tRNA_ribosyl_Ptfrase_RIT1. [Graphical view]
Pfam	PF04179. Init_tRNA_PT. 1 hit. [Graphical view]
PIRSF	PIRSF007747. Ribosyl_Ptfrase. 1 hit.
ProtoNet	Search...
Other
NextBio	979041.

Entry information

Entry name

RIT1_YEAST

Accession

Primary (citable) accession number: P23796
Secondary accession number(s): D6W0B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program