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P23796

- RIT1_YEAST

UniProt

P23796 - RIT1_YEAST

Protein

tRNA A64-2'-O-ribosylphosphate transferase

Gene

RIT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 3 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    tRNA backbone modifying enzyme that mediates initiator/ elongator tRNA discrimination. This enzyme modifies exclusively the initiator tRNA in position 64 using 5'-phosphoribosyl-1'-pyrophosphate as the modification donor. Recognize the stem-loop IV region that is unique in eukaryotic cytoplasmic initiator tRNAs.

    GO - Molecular functioni

    1. transferase activity, transferring pentosyl groups Source: SGD

    GO - Biological processi

    1. charged-tRNA amino acid modification Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32953-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA A64-2'-O-ribosylphosphate transferase (EC:2.4.2.-)
    Alternative name(s):
    Initiator tRNA phosphoribosyl-transferase
    Gene namesi
    Name:RIT1
    Ordered Locus Names:YMR283C
    ORF Names:YM8021.09C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR283c.
    SGDiS000004896. RIT1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 513513tRNA A64-2'-O-ribosylphosphate transferasePRO_0000097353Add
    BLAST

    Proteomic databases

    MaxQBiP23796.
    PaxDbiP23796.

    Expressioni

    Gene expression databases

    GenevestigatoriP23796.

    Interactioni

    Protein-protein interaction databases

    BioGridi35462. 51 interactions.
    MINTiMINT-4498956.
    STRINGi4932.YMR283C.

    Structurei

    3D structure databases

    ProteinModelPortaliP23796.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG241557.
    HOGENOMiHOG000174281.
    KOiK15463.
    OMAiSADDHEL.
    OrthoDBiEOG7B05P8.

    Family and domain databases

    InterProiIPR007306. Init_tRNA_PT.
    IPR016519. tRNA_ribosyl_Ptfrase_RIT1.
    [Graphical view]
    PfamiPF04179. Init_tRNA_PT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF007747. Ribosyl_Ptfrase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P23796-1 [UniParc]FASTAAdd to Basket

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    MVSRLSKRYR SRVQFSISVM DENIYLSLSQ INKDIRKENK SVRNRLQSIL    50
    LDNKFLQDRV IPIFPHYPLI PNERCGLWYC NPSSFKQTSY FKSTDGHVNQ 100
    WDFSTRRLNF HLLETIRDNK GIIIVDSTRR GKKIPDALSK TVPIWCAVLN 150
    TLMLQETEKN VAIDKVLYLP PETVPKSEYD MIKRKIPELV AKLQKLNIID 200
    SKKLNELFMG KLLRPIWVHP GSSLLDHSVD YFTGEVQEYE AWETPEDQNI 250
    IPIILCTVSY QAQDGMDKRY GFTYVQGAAD DHELWSFGLD SNMFWAHIEY 300
    LGDASYSDDQ LHDYIMDLAA AKLRNQCYIQ DKGSLDEVFG NIDKITNEIS 350
    LGKVSSGLTI NKNLKQKLKS EYGKVIIFSN SVTVAEDTDD EEESGTDPFI 400
    SIYKLQSGDK KSSKALRSTF PRIHGEIQSL FTNRDEKIKP MLICCNTGTD 450
    MSIGVILSIL CTKYTEEWML TSELPDISKL IVRKHLTKLI SHLKGRNVNP 500
    SRATLNSVNS FLM 513
    Length:513
    Mass (Da):58,882
    Last modified:February 1, 1996 - v3
    Checksum:i36F97B2445A4EA09
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80795 Genomic DNA. Translation: CAA56770.1.
    Z49704 Genomic DNA. Translation: CAA89781.1.
    AY692815 Genomic DNA. Translation: AAT92834.1.
    M59860 Genomic DNA. No translation available.
    BK006946 Genomic DNA. Translation: DAA10184.1.
    PIRiS54590.
    RefSeqiNP_014010.1. NM_001182790.1.

    Genome annotation databases

    EnsemblFungiiYMR283C; YMR283C; YMR283C.
    GeneIDi855326.
    KEGGisce:YMR283C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80795 Genomic DNA. Translation: CAA56770.1 .
    Z49704 Genomic DNA. Translation: CAA89781.1 .
    AY692815 Genomic DNA. Translation: AAT92834.1 .
    M59860 Genomic DNA. No translation available.
    BK006946 Genomic DNA. Translation: DAA10184.1 .
    PIRi S54590.
    RefSeqi NP_014010.1. NM_001182790.1.

    3D structure databases

    ProteinModelPortali P23796.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35462. 51 interactions.
    MINTi MINT-4498956.
    STRINGi 4932.YMR283C.

    Proteomic databases

    MaxQBi P23796.
    PaxDbi P23796.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR283C ; YMR283C ; YMR283C .
    GeneIDi 855326.
    KEGGi sce:YMR283C.

    Organism-specific databases

    CYGDi YMR283c.
    SGDi S000004896. RIT1.

    Phylogenomic databases

    eggNOGi NOG241557.
    HOGENOMi HOG000174281.
    KOi K15463.
    OMAi SADDHEL.
    OrthoDBi EOG7B05P8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32953-MONOMER.

    Miscellaneous databases

    NextBioi 979041.

    Gene expression databases

    Genevestigatori P23796.

    Family and domain databases

    InterProi IPR007306. Init_tRNA_PT.
    IPR016519. tRNA_ribosyl_Ptfrase_RIT1.
    [Graphical view ]
    Pfami PF04179. Init_tRNA_PT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF007747. Ribosyl_Ptfrase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination."
      Aastroem S.U., Bystroem A.S.
      Cell 79:535-546(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-513.
      Strain: S288c / YPH1.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Characterization of a yeast nuclear gene, AEP2, required for accumulation of mitochondrial mRNA encoding subunit 9 of the ATP synthase."
      Payne M.J., Finnegan P.M., Keramidaris E., Lukins H.B.
      Curr. Genet. 20:53-61(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 418-513.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRIT1_YEAST
    AccessioniPrimary (citable) accession number: P23796
    Secondary accession number(s): D6W0B0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 103 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 259 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3