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P23796 (RIT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA A64-2'-O-ribosylphosphate transferase

EC=2.4.2.-
Alternative name(s):
Initiator tRNA phosphoribosyl-transferase
Gene names
Name:RIT1
Ordered Locus Names:YMR283C
ORF Names:YM8021.09C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

tRNA backbone modifying enzyme that mediates initiator/ elongator tRNA discrimination. This enzyme modifies exclusively the initiator tRNA in position 64 using 5'-phosphoribosyl-1'-pyrophosphate as the modification donor. Recognize the stem-loop IV region that is unique in eukaryotic cytoplasmic initiator tRNAs.

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 513513tRNA A64-2'-O-ribosylphosphate transferase
PRO_0000097353

Sequences

Sequence LengthMass (Da)Tools
P23796 [UniParc].

Last modified February 1, 1996. Version 3.
Checksum: 36F97B2445A4EA09

FASTA51358,882
        10         20         30         40         50         60 
MVSRLSKRYR SRVQFSISVM DENIYLSLSQ INKDIRKENK SVRNRLQSIL LDNKFLQDRV 

        70         80         90        100        110        120 
IPIFPHYPLI PNERCGLWYC NPSSFKQTSY FKSTDGHVNQ WDFSTRRLNF HLLETIRDNK 

       130        140        150        160        170        180 
GIIIVDSTRR GKKIPDALSK TVPIWCAVLN TLMLQETEKN VAIDKVLYLP PETVPKSEYD 

       190        200        210        220        230        240 
MIKRKIPELV AKLQKLNIID SKKLNELFMG KLLRPIWVHP GSSLLDHSVD YFTGEVQEYE 

       250        260        270        280        290        300 
AWETPEDQNI IPIILCTVSY QAQDGMDKRY GFTYVQGAAD DHELWSFGLD SNMFWAHIEY 

       310        320        330        340        350        360 
LGDASYSDDQ LHDYIMDLAA AKLRNQCYIQ DKGSLDEVFG NIDKITNEIS LGKVSSGLTI 

       370        380        390        400        410        420 
NKNLKQKLKS EYGKVIIFSN SVTVAEDTDD EEESGTDPFI SIYKLQSGDK KSSKALRSTF 

       430        440        450        460        470        480 
PRIHGEIQSL FTNRDEKIKP MLICCNTGTD MSIGVILSIL CTKYTEEWML TSELPDISKL 

       490        500        510 
IVRKHLTKLI SHLKGRNVNP SRATLNSVNS FLM 

« Hide

References

« Hide 'large scale' references
[1]"Rit1, a tRNA backbone-modifying enzyme that mediates initiator and elongator tRNA discrimination."
Aastroem S.U., Bystroem A.S.
Cell 79:535-546(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 20-513.
Strain: S288c / YPH1.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Characterization of a yeast nuclear gene, AEP2, required for accumulation of mitochondrial mRNA encoding subunit 9 of the ATP synthase."
Payne M.J., Finnegan P.M., Keramidaris E., Lukins H.B.
Curr. Genet. 20:53-61(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 418-513.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X80795 Genomic DNA. Translation: CAA56770.1.
Z49704 Genomic DNA. Translation: CAA89781.1.
AY692815 Genomic DNA. Translation: AAT92834.1.
M59860 Genomic DNA. No translation available.
BK006946 Genomic DNA. Translation: DAA10184.1.
PIRS54590.
RefSeqNP_014010.1. NM_001182790.1.

3D structure databases

ProteinModelPortalP23796.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35462. 51 interactions.
MINTMINT-4498956.
STRING4932.YMR283C.

Proteomic databases

PaxDbP23796.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR283C; YMR283C; YMR283C.
GeneID855326.
KEGGsce:YMR283C.

Organism-specific databases

CYGDYMR283c.
SGDS000004896. RIT1.

Phylogenomic databases

eggNOGNOG241557.
HOGENOMHOG000174281.
KOK15463.
OMASADDHEL.
OrthoDBEOG7B05P8.

Enzyme and pathway databases

BioCycYEAST:G3O-32953-MONOMER.

Gene expression databases

GenevestigatorP23796.

Family and domain databases

InterProIPR007306. Init_tRNA_PT.
IPR016519. tRNA_ribosyl_Ptfrase_RIT1.
[Graphical view]
PfamPF04179. Init_tRNA_PT. 1 hit.
[Graphical view]
PIRSFPIRSF007747. Ribosyl_Ptfrase. 1 hit.
ProtoNetSearch...

Other

NextBio979041.

Entry information

Entry nameRIT1_YEAST
AccessionPrimary (citable) accession number: P23796
Secondary accession number(s): D6W0B0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD