P23795 (ACES_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylcholinesterase
Short name=AChE
EC=3.1.1.7

Gene names

Name:

ACHE

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Catalytic activity	Acetylcholine + H2O = choline + acetate.
Subunit structure	Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers By similarity. Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers.
Subcellular location	Cell junction › synapse. Secreted. Cell membrane; Peripheral membrane protein By similarity. Isoform H: Cell membrane; Lipid-anchor › GPI-anchor; Extracellular side.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Biological process	Neurotransmitter degradation
Cellular component	Cell junction Cell membrane Membrane Secreted Synapse
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase Serine esterase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	choline metabolic process Inferred from Biological aspect of Ancestor. Source: RefGenome neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of axonogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of dendrite morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome synapse assembly Inferred from Biological aspect of Ancestor. Source: RefGenome synaptic transmission, cholinergic Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW axon Inferred from Biological aspect of Ancestor. Source: RefGenome cell junction Inferred from electronic annotation. Source: UniProtKB-KW cell surface Inferred from Biological aspect of Ancestor. Source: RefGenome dendrite Inferred from Biological aspect of Ancestor. Source: RefGenome endoplasmic reticulum lumen Inferred from Biological aspect of Ancestor. Source: RefGenome extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome neuromuscular junction Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from Biological aspect of Ancestor. Source: RefGenome presynaptic membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular function	acetylcholinesterase activity Inferred from Biological aspect of Ancestor. Source: RefGenome beta-amyloid binding Inferred from direct assay. Source: HGNC carboxylesterase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform T (identifier: P23795-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform H (identifier: P23795-2)

The sequence of this isoform differs from the canonical sequence as follows:
     574-613: DTLDEAERQW...YSKQDRCSDL → ASEAPCTCSG...LFLLSRLLRL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Ref.3
	Chain	31 – 613	583	Acetylcholinesterase		PRO_0000008585
Sites
	Active site	233	1	Acyl-ester intermediate By similarity
	Active site	364	1	Charge relay system By similarity
	Active site	477	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	91	1	N-linked (GlcNAc...) Probable
	Glycosylation	295	1	N-linked (GlcNAc...) Probable
	Glycosylation	380	1	N-linked (GlcNAc...) Probable
	Glycosylation	494	1	N-linked (GlcNAc...) Probable
	Disulfide bond	99 ↔ 126		By similarity
	Disulfide bond	287 ↔ 302		By similarity
	Disulfide bond	439 ↔ 559		By similarity
	Disulfide bond	610		Interchain By similarity
Natural variations
	Alternative sequence	574 – 613	40	DTLDE…RCSDL → ASEAPCTCSGPAHGEAAPRP RPGLPLPLLLLLFLLSRLLR L in isoform H.		VSP_001455
Experimental info
	Sequence conflict	24	1	I → L in AAI23899. Ref.2
	Sequence conflict	46	1	R → E AA sequence Ref.3
	Sequence conflict	169	1	T → V AA sequence Ref.3
	Sequence conflict	212	1	W → S AA sequence Ref.3
	Sequence conflict	323	1	S → H AA sequence Ref.3
	Sequence conflict	352	1	H → V AA sequence Ref.3
	Sequence conflict	424	1	L → W AA sequence Ref.3
	Sequence conflict	524	1	D → A AA sequence Ref.3
	Sequence conflict	549 – 554	6	EVRRGL → GVPQAS AA sequence Ref.3
	Sequence conflict	571	1	S → N AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform T [UniParc]. Last modified July 11, 2001. Version 2. Checksum: 698D4F0DF8624B12	FASTA	613	67,664
	10 20 30 40 50 60 MRPPWCPLHT PSLTPPLLLL LFLIGGGAEA EGPEDPELLV MVRGGRLRGL RLMAPRGPVS 70 80 90 100 110 120 AFLGIPFAEP PVGPRRFLPP EPKRPWPGVL NATAFQSVCY QYVDTLYPGF EGTEMWNPNR 130 140 150 160 170 180 ELSEDCLYLN VWTPYPRPSS PTPVLVWIYG GGFYSGASSL DVYDGRFLTQ AEGTVLVSMN 190 200 210 220 230 240 YRVGAFGFLA LPGSREAPGN VGLLDQRLAL QWVQENVAAF GGDPTSVTLF GESAGAASVG 250 260 270 280 290 300 MHLLSPPSRG LFHRAVLQSG APNGPWATVG VGEARRRATL LARLVGCPPG GAGGNDTELV 310 320 330 340 350 360 ACLRARPAQD LVDHEWRVLP QESVFRFSFV PVVDGDFLSD TPEALINAGD FHGLQVLVGV 370 380 390 400 410 420 VKDEGSYFLV YGAPGFSKDN ESLISRAQFL AGVRVGVPQA SDLAAEAVVL HYTDWLHPED 430 440 450 460 470 480 PARLREALSD VVGDHNVVCP VAQLAGRLAA QGARVYAYIF EHRASTLSWP LWMGVPHGYE 490 500 510 520 530 540 IEFIFGLPLE PSLNYTIEER TFAQRLMRYW ANFARTGDPN DPRDPKAPQW PPYTAGAQQY 550 560 570 580 590 600 VSLNLRPLEV RRGLRAQACA FWNRFLPKLL SATDTLDEAE RQWKAEFHRW SSYMVHWKNQ 610 FDHYSKQDRC SDL
	Isoform H [UniParc]. Checksum: 444F4D6D0EDFDAE3	FASTA	614	66,908
	10 20 30 40 50 60 MRPPWCPLHT PSLTPPLLLL LFLIGGGAEA EGPEDPELLV MVRGGRLRGL RLMAPRGPVS 70 80 90 100 110 120 AFLGIPFAEP PVGPRRFLPP EPKRPWPGVL NATAFQSVCY QYVDTLYPGF EGTEMWNPNR 130 140 150 160 170 180 ELSEDCLYLN VWTPYPRPSS PTPVLVWIYG GGFYSGASSL DVYDGRFLTQ AEGTVLVSMN 190 200 210 220 230 240 YRVGAFGFLA LPGSREAPGN VGLLDQRLAL QWVQENVAAF GGDPTSVTLF GESAGAASVG 250 260 270 280 290 300 MHLLSPPSRG LFHRAVLQSG APNGPWATVG VGEARRRATL LARLVGCPPG GAGGNDTELV 310 320 330 340 350 360 ACLRARPAQD LVDHEWRVLP QESVFRFSFV PVVDGDFLSD TPEALINAGD FHGLQVLVGV 370 380 390 400 410 420 VKDEGSYFLV YGAPGFSKDN ESLISRAQFL AGVRVGVPQA SDLAAEAVVL HYTDWLHPED 430 440 450 460 470 480 PARLREALSD VVGDHNVVCP VAQLAGRLAA QGARVYAYIF EHRASTLSWP LWMGVPHGYE 490 500 510 520 530 540 IEFIFGLPLE PSLNYTIEER TFAQRLMRYW ANFARTGDPN DPRDPKAPQW PPYTAGAQQY 550 560 570 580 590 600 VSLNLRPLEV RRGLRAQACA FWNRFLPKLL SATASEAPCT CSGPAHGEAA PRPRPGLPLP 610 LLLLLFLLSR LLRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bovine acetylcholinesterase: cloning, expression and characterization." Mendelson I., Kronman C., Ariel N., Shafferman A., Velan B. Biochem. J. 334:251-259(1998) [PubMed: 9693127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Tissue: Kidney.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM T). Strain: Hereford. Tissue: Basal ganglia.
[3]	"Complete amino acid sequence of fetal bovine serum acetylcholinesterase and its comparison in various regions with other cholinesterases." Doctor B.P., Chapman T.C., Christner C.E., Deal C.D., de la Hoz D.M., Gentry M.K., Ogert R.A., Rush R.S., Smyth K.K., Wolfe A.D. FEBS Lett. 266:123-127(1990) [PubMed: 2365060] [Abstract] Cited for: PROTEIN SEQUENCE OF 31-613 (ISOFORM H). Tissue: Fetal serum.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF061815, AF061813, AF061814 Genomic DNA. Translation: AAC64270.1. BC123898 mRNA. Translation: AAI23899.1.
IPI	IPI00690780. IPI00711550.
RefSeq	NP_001069688.1. NM_001076220.1.
UniGene	Bt.1299.
3D structure databases
ProteinModelPortal	P23795.
SMR	P23795. Positions 35-607.
ModBase	Search...
Protein-protein interaction databases
STRING	P23795.
Protein family/group databases
MEROPS	S09.979.
PTM databases
GlycoSuiteDB	P23795.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000001512; ENSBTAP00000001512; ENSBTAG00000001139.
GeneID	540446.
KEGG	bta:540446.
Organism-specific databases
CTD	43.
Phylogenomic databases
eggNOG	maNOG13227.
HOVERGEN	HBG008839.
InParanoid	P23795.
OrthoDB	EOG46WZ86.
Family and domain databases
InterPro	IPR014788. AChE_tetra. IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. IPR019819. Carboxylesterase_B_CS. IPR000997. Cholinesterase. [Graphical view]
KO	K01049.
Pfam	PF08674. AChE_tetra. 1 hit. PF00135. COesterase. 1 hit. [Graphical view]
PRINTS	PR00878. CHOLNESTRASE.
ProDom	PD415333. AChE_tetra. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. PS00941. CARBOXYLESTERASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACES_BOVIN

Accession

Primary (citable) accession number: P23795
Secondary accession number(s): O97579, Q08D79

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	July 11, 2001
Last modified:	November 16, 2011
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program