Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P23795

- ACES_BOVIN

UniProt

P23795 - ACES_BOVIN

Protein

Acetylcholinesterase

Gene

ACHE

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (11 Jul 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.

    Catalytic activityi

    Acetylcholine + H2O = choline + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei233 – 2331Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei364 – 3641Charge relay systemBy similarity
    Active sitei477 – 4771Charge relay systemBy similarity

    GO - Molecular functioni

    1. acetylcholinesterase activity Source: RefGenome
    2. beta-amyloid binding Source: HGNC
    3. cholinesterase activity Source: RefGenome
    4. protein binding Source: HGNC

    GO - Biological processi

    1. choline metabolic process Source: RefGenome
    2. neurotransmitter catabolic process Source: UniProtKB-KW
    3. regulation of axonogenesis Source: RefGenome
    4. regulation of dendrite morphogenesis Source: RefGenome
    5. synapse assembly Source: RefGenome
    6. synaptic transmission, cholinergic Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Keywords - Biological processi

    Neurotransmitter degradation

    Enzyme and pathway databases

    ReactomeiREACT_223134. Neurotransmitter Clearance In The Synaptic Cleft.
    REACT_223586. Synthesis of PC.

    Protein family/group databases

    MEROPSiS09.979.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetylcholinesterase (EC:3.1.1.7)
    Short name:
    AChE
    Gene namesi
    Name:ACHE
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 25

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. axon Source: RefGenome
    3. cell junction Source: UniProtKB-KW
    4. cell surface Source: RefGenome
    5. dendrite Source: RefGenome
    6. endoplasmic reticulum lumen Source: RefGenome
    7. extracellular space Source: RefGenome
    8. neuromuscular junction Source: RefGenome
    9. postsynaptic membrane Source: RefGenome
    10. presynaptic membrane Source: RefGenome

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Secreted, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Add
    BLAST
    Chaini31 – 613583AcetylcholinesterasePRO_0000008585Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi91 – 911N-linked (GlcNAc...)Curated
    Disulfide bondi99 ↔ 126By similarity
    Disulfide bondi287 ↔ 302By similarity
    Glycosylationi295 – 2951N-linked (GlcNAc...)Curated
    Glycosylationi380 – 3801N-linked (GlcNAc...)Curated
    Disulfide bondi439 ↔ 559By similarity
    Glycosylationi494 – 4941N-linked (GlcNAc...)Curated
    Disulfide bondi610 – 610InterchainBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    PTM databases

    UniCarbKBiP23795.

    Interactioni

    Subunit structurei

    Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers By similarity. Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP23795.
    SMRiP23795. Positions 35-607.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2272.
    GeneTreeiENSGT00740000115241.
    HOGENOMiHOG000091866.
    HOVERGENiHBG008839.
    InParanoidiP23795.
    KOiK01049.
    OrthoDBiEOG789C9R.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR014788. AChE_tetra.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    IPR000997. Cholinesterase.
    [Graphical view]
    PfamiPF08674. AChE_tetra. 1 hit.
    PF00135. COesterase. 1 hit.
    [Graphical view]
    PRINTSiPR00878. CHOLNESTRASE.
    ProDomiPD415333. AChE_tetra. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform T (identifier: P23795-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRPPWCPLHT PSLTPPLLLL LFLIGGGAEA EGPEDPELLV MVRGGRLRGL    50
    RLMAPRGPVS AFLGIPFAEP PVGPRRFLPP EPKRPWPGVL NATAFQSVCY 100
    QYVDTLYPGF EGTEMWNPNR ELSEDCLYLN VWTPYPRPSS PTPVLVWIYG 150
    GGFYSGASSL DVYDGRFLTQ AEGTVLVSMN YRVGAFGFLA LPGSREAPGN 200
    VGLLDQRLAL QWVQENVAAF GGDPTSVTLF GESAGAASVG MHLLSPPSRG 250
    LFHRAVLQSG APNGPWATVG VGEARRRATL LARLVGCPPG GAGGNDTELV 300
    ACLRARPAQD LVDHEWRVLP QESVFRFSFV PVVDGDFLSD TPEALINAGD 350
    FHGLQVLVGV VKDEGSYFLV YGAPGFSKDN ESLISRAQFL AGVRVGVPQA 400
    SDLAAEAVVL HYTDWLHPED PARLREALSD VVGDHNVVCP VAQLAGRLAA 450
    QGARVYAYIF EHRASTLSWP LWMGVPHGYE IEFIFGLPLE PSLNYTIEER 500
    TFAQRLMRYW ANFARTGDPN DPRDPKAPQW PPYTAGAQQY VSLNLRPLEV 550
    RRGLRAQACA FWNRFLPKLL SATDTLDEAE RQWKAEFHRW SSYMVHWKNQ 600
    FDHYSKQDRC SDL 613
    Length:613
    Mass (Da):67,664
    Last modified:July 11, 2001 - v2
    Checksum:i698D4F0DF8624B12
    GO
    Isoform H (identifier: P23795-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         574-613: DTLDEAERQW...YSKQDRCSDL → ASEAPCTCSG...LFLLSRLLRL

    Show »
    Length:614
    Mass (Da):66,908
    Checksum:i444F4D6D0EDFDAE3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241I → L in AAI23899. 1 PublicationCurated
    Sequence conflicti46 – 461R → E AA sequence (PubMed:2365060)Curated
    Sequence conflicti169 – 1691T → V AA sequence (PubMed:2365060)Curated
    Sequence conflicti212 – 2121W → S AA sequence (PubMed:2365060)Curated
    Sequence conflicti323 – 3231S → H AA sequence (PubMed:2365060)Curated
    Sequence conflicti352 – 3521H → V AA sequence (PubMed:2365060)Curated
    Sequence conflicti424 – 4241L → W AA sequence (PubMed:2365060)Curated
    Sequence conflicti524 – 5241D → A AA sequence (PubMed:2365060)Curated
    Sequence conflicti549 – 5546EVRRGL → GVPQAS AA sequence (PubMed:2365060)Curated
    Sequence conflicti571 – 5711S → N AA sequence (PubMed:2365060)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei574 – 61340DTLDE…RCSDL → ASEAPCTCSGPAHGEAAPRP RPGLPLPLLLLLFLLSRLLR L in isoform H. CuratedVSP_001455Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061815, AF061813, AF061814 Genomic DNA. Translation: AAC64270.1.
    BC123898 mRNA. Translation: AAI23899.1.
    RefSeqiNP_001069688.1. NM_001076220.1.
    UniGeneiBt.1299.

    Genome annotation databases

    EnsembliENSBTAT00000001512; ENSBTAP00000001512; ENSBTAG00000001139.
    GeneIDi540446.
    KEGGibta:540446.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061815 , AF061813 , AF061814 Genomic DNA. Translation: AAC64270.1 .
    BC123898 mRNA. Translation: AAI23899.1 .
    RefSeqi NP_001069688.1. NM_001076220.1.
    UniGenei Bt.1299.

    3D structure databases

    ProteinModelPortali P23795.
    SMRi P23795. Positions 35-607.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P23795.
    ChEMBLi CHEMBL4768.

    Protein family/group databases

    MEROPSi S09.979.

    PTM databases

    UniCarbKBi P23795.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000001512 ; ENSBTAP00000001512 ; ENSBTAG00000001139 .
    GeneIDi 540446.
    KEGGi bta:540446.

    Organism-specific databases

    CTDi 43.

    Phylogenomic databases

    eggNOGi COG2272.
    GeneTreei ENSGT00740000115241.
    HOGENOMi HOG000091866.
    HOVERGENi HBG008839.
    InParanoidi P23795.
    KOi K01049.
    OrthoDBi EOG789C9R.

    Enzyme and pathway databases

    Reactomei REACT_223134. Neurotransmitter Clearance In The Synaptic Cleft.
    REACT_223586. Synthesis of PC.

    Miscellaneous databases

    NextBioi 20878642.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR014788. AChE_tetra.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    IPR000997. Cholinesterase.
    [Graphical view ]
    Pfami PF08674. AChE_tetra. 1 hit.
    PF00135. COesterase. 1 hit.
    [Graphical view ]
    PRINTSi PR00878. CHOLNESTRASE.
    ProDomi PD415333. AChE_tetra. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bovine acetylcholinesterase: cloning, expression and characterization."
      Mendelson I., Kronman C., Ariel N., Shafferman A., Velan B.
      Biochem. J. 334:251-259(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
      Tissue: Kidney.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM T).
      Strain: Hereford.
      Tissue: Basal ganglia.
    3. "Complete amino acid sequence of fetal bovine serum acetylcholinesterase and its comparison in various regions with other cholinesterases."
      Doctor B.P., Chapman T.C., Christner C.E., Deal C.D., de la Hoz D.M., Gentry M.K., Ogert R.A., Rush R.S., Smyth K.K., Wolfe A.D.
      FEBS Lett. 266:123-127(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 31-613 (ISOFORM H).
      Tissue: Fetal serum.

    Entry informationi

    Entry nameiACES_BOVIN
    AccessioniPrimary (citable) accession number: P23795
    Secondary accession number(s): O97579, Q08D79
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: July 11, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3