P23794 (GLNA_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine synthetase
Short name=GS
EC=6.3.1.2

Alternative name(s):
Glutamate--ammonia ligase

Gene names

Name:	glnA
Synonyms:	glnA-1
Ordered Locus Names:	SSO0366

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus solfataricus

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
Enzyme regulation	The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro nitrogen fixation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Inferred from electronic annotation. Source: UniProtKB-EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Glutamine synthetase		PRO_0000153214
Amino acid modifications
	Modified residue	400	1	O-AMP-tyrosine By similarity
Experimental info
	Sequence conflict	162	1	S → T in CAA37352. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P23794 [UniParc]. Last modified June 20, 2001. Version 2. Checksum: 1CC4239287B6C2CD	FASTA	471	53,348
	10 20 30 40 50 60 MPSTAEDVLK FLKENNIKWV DLQFTDVPGR LHHITIPADE FDLESLKTGF GKLDGSSIRG 70 80 90 100 110 120 FTSIYESDMV LLPVPETMTL IPWSPGVARV LCKVFWGGGK GRFERDPRFI AEEAEKYQTE 130 140 150 160 170 180 QGYTSYYGPE LEFFMFDKVK LDVSAPQSGT GYKIYAREAP WSDSGTFVIR FKEGYYPAPP 190 200 210 220 230 240 VDQLMDVRVE IVDTLVKYFG YTIEATHHEV ATAGQGEIDF RFSTLVDTAD KVQTLKYVAK 250 260 270 280 290 300 NIAAKHGLVV TFMPKPIYGD NGTGMHTHLS LWTKDGKKNL MYDPNDEYAE ISQFGRYVIG 310 320 330 340 350 360 GLLTHARALS AIVSPTVNSY RRLIPGFEAP VYIAWSKGNR SAVIRVPAYY RGMEKAKRIE 370 380 390 400 410 420 YRPPDPSTNP YLAFAALLMA ALDGVNKKID PGDPVDENIY HLTPEKRRQL GIKELPRSLN 430 440 450 460 470 EALDELESDK EFLKPVFNSS ILDTYIDLKR DEARSLQGYP HPMELYYYLD S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of an archaebacterial glutamine synthetase gene: phylogenetic implications." Sanangelantoni A.M., Barbarini D., di Pasquale G., Cammarano P., Tiboni O. Mol. Gen. Genet. 221:187-194(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53263 Genomic DNA. Translation: CAA37352.1. AE006641 Genomic DNA. Translation: AAK40696.1.
PIR	A99180. S11899.
RefSeq	NP_341906.1. NC_002754.1.
3D structure databases
ProteinModelPortal	P23794.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	273057.SSO0366.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK40696; AAK40696; SSO0366.
GeneID	1455506.
KEGG	sso:SSO0366.
Phylogenomic databases
eggNOG	COG0174.
HOGENOM	HOG000005157.
KO	K01915.
OMA	VHAWEIE.
Enzyme and pathway databases
BioCyc	SSOL273057:GCH2-349-MONOMER.
Family and domain databases
Gene3D	3.10.20.70. 1 hit. 3.30.590.10. 1 hit.
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat_dom. IPR008146. Gln_synth_cat_dom. IPR027303. Gln_synth_gly_rich_site. IPR004809. Gln_synth_I. IPR027302. Gln_synth_N_conserv_site. [Graphical view]
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
SUPFAM	SSF54368. SSF54368. 1 hit.
TIGRFAMs	TIGR00653. GlnA. 1 hit.
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNA_SULSO

Accession

Primary (citable) accession number: P23794

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	June 20, 2001
Last modified:	May 14, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program