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P23780

- BGAL_MOUSE

UniProt

P23780 - BGAL_MOUSE

Protein

Beta-galactosidase

Gene

Glb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei189 – 1891Proton donorSequence Analysis
    Active sitei269 – 2691NucleophileSequence Analysis

    GO - Molecular functioni

    1. beta-galactosidase activity Source: MGI
    2. galactoside binding Source: Ensembl

    GO - Biological processi

    1. galactose catabolic process Source: Ensembl

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    ReactomeiREACT_198960. Keratan sulfate degradation.
    REACT_198967. HS-GAG degradation.
    REACT_199008. Glycosphingolipid metabolism.
    REACT_202500. Sialic acid metabolism.

    Protein family/group databases

    CAZyiGH35. Glycoside Hydrolase Family 35.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase (EC:3.2.1.23)
    Alternative name(s):
    Acid beta-galactosidase
    Short name:
    Lactase
    Gene namesi
    Name:Glb1
    Synonyms:Bgl, Glb-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:88151. Glb1.

    Subcellular locationi

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 295By similarityPRO_0000012190
    Chaini30 – 647618Beta-galactosidasePRO_0000012191Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi27 – 271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi500 – 5001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi504 – 5041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi510 – 5101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi544 – 5441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi557 – 5571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi617 – 6171N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiP23780.
    PaxDbiP23780.
    PRIDEiP23780.

    PTM databases

    PhosphoSiteiP23780.

    Expressioni

    Gene expression databases

    ArrayExpressiP23780.
    BgeeiP23780.
    CleanExiMM_GLB1.
    GenevestigatoriP23780.

    Interactioni

    Protein-protein interaction databases

    BioGridi198341. 1 interaction.
    IntActiP23780. 1 interaction.
    MINTiMINT-4089206.

    Structurei

    3D structure databases

    ProteinModelPortaliP23780.
    SMRiP23780. Positions 30-646.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000221607.
    HOVERGENiHBG004841.
    InParanoidiP23780.
    KOiK12309.
    OMAiRIPRVYW.
    OrthoDBiEOG7GXPCD.
    PhylomeDBiP23780.
    TreeFamiTF314816.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR026283. B-gal_1-like.
    IPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006336. B-gal. 1 hit.
    PRINTSiPR00742. GLHYDRLASE35.
    SUPFAMiSSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23780-1 [UniParc]FASTAAdd to Basket

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    MLRVPLCTPL PLLALLQLLG AAHGIYNVTQ RTFKLDYSRD RFLKDGQPFR    50
    YISGSIHYFR IPRFYWEDRL LKMKMAGLNA IQMYVPWNFH EPQPGQYEFS 100
    GDRDVEHFIQ LAHELGLLVI LRPGPYICAE WDMGGLPAWL LEKQSIVLRS 150
    SDPDYLVAVD KWLAVLLPKM KPLLYQNGGP IITVQVENEY GSYFACDYDY 200
    LRFLVHRFRY HLGNDVILFT TDGASEKMLK CGTLQDLYAT VDFGTGNNIT 250
    QAFLVQRKFE PKGPLINSEF YTGWLDHWGK PHSTVKTKTL ATSLYNLLAR 300
    GANVNLYMFI GGTNFAYWNG ANTPYEPQPT SYDYDAPLSE AGDLTKKYFA 350
    LREVIQMFKE VPEGPIPPST PKFAYGKVAL RKFKTVAEAL GILCPNGPVK 400
    SLYPLTFTQV KQYFGYVLYR TTLPQDCSNP KPIFSSPFNG VRDRAYVSVD 450
    GVPQGILDRN LMTALNIRGK AGATLDILVE NMGRVNYGRF INDFKGLISN 500
    MTINSTVLTN WTVFPLNTEA MVRNHLWGRE ASDEGHLDGR STSNSSDLIL 550
    PTFYVGNFSI PSGIPDLPQD TFIQFPGWSK GQVWINGFNL GRYWPTMGPQ 600
    KTLFVPRNIL TTSAPNNITV LELEFAPCSE GTPELCTVEF VDTPVIS 647
    Length:647
    Mass (Da):73,121
    Last modified:November 1, 1991 - v1
    Checksum:i0E68EAA66A10803A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti517 – 5171N → D in AAA37292. (PubMed:1906271)Curated
    Sequence conflicti539 – 5391G → R in AAA37292. (PubMed:1906271)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57734 mRNA. Translation: AAA37293.1.
    M75122
    , M75137, M75107, M75108, M75109, M75111, M75112, M75113, M75114, M75115, M75116, M75117, M75118, M75119, M75120, M75121 Genomic DNA. Translation: AAA37292.1.
    CCDSiCCDS23593.1.
    PIRiA37086.
    RefSeqiNP_033882.1. NM_009752.2.
    UniGeneiMm.290516.

    Genome annotation databases

    EnsembliENSMUST00000063042; ENSMUSP00000055803; ENSMUSG00000045594.
    GeneIDi12091.
    KEGGimmu:12091.
    UCSCiuc009rxj.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57734 mRNA. Translation: AAA37293.1 .
    M75122
    , M75137 , M75107 , M75108 , M75109 , M75111 , M75112 , M75113 , M75114 , M75115 , M75116 , M75117 , M75118 , M75119 , M75120 , M75121 Genomic DNA. Translation: AAA37292.1 .
    CCDSi CCDS23593.1.
    PIRi A37086.
    RefSeqi NP_033882.1. NM_009752.2.
    UniGenei Mm.290516.

    3D structure databases

    ProteinModelPortali P23780.
    SMRi P23780. Positions 30-646.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198341. 1 interaction.
    IntActi P23780. 1 interaction.
    MINTi MINT-4089206.

    Chemistry

    BindingDBi P23780.
    ChEMBLi CHEMBL1667667.

    Protein family/group databases

    CAZyi GH35. Glycoside Hydrolase Family 35.

    PTM databases

    PhosphoSitei P23780.

    Proteomic databases

    MaxQBi P23780.
    PaxDbi P23780.
    PRIDEi P23780.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000063042 ; ENSMUSP00000055803 ; ENSMUSG00000045594 .
    GeneIDi 12091.
    KEGGi mmu:12091.
    UCSCi uc009rxj.2. mouse.

    Organism-specific databases

    CTDi 2720.
    MGIi MGI:88151. Glb1.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000221607.
    HOVERGENi HBG004841.
    InParanoidi P23780.
    KOi K12309.
    OMAi RIPRVYW.
    OrthoDBi EOG7GXPCD.
    PhylomeDBi P23780.
    TreeFami TF314816.

    Enzyme and pathway databases

    Reactomei REACT_198960. Keratan sulfate degradation.
    REACT_198967. HS-GAG degradation.
    REACT_199008. Glycosphingolipid metabolism.
    REACT_202500. Sialic acid metabolism.

    Miscellaneous databases

    NextBioi 280407.
    PROi P23780.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P23780.
    Bgeei P23780.
    CleanExi MM_GLB1.
    Genevestigatori P23780.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR026283. B-gal_1-like.
    IPR008979. Galactose-bd-like.
    IPR019801. Glyco_hydro_35_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006336. B-gal. 1 hit.
    PRINTSi PR00742. GLHYDRLASE35.
    SUPFAMi SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    PROSITEi PS01182. GLYCOSYL_HYDROL_F35. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of mouse acid beta-galactosidase cDNA: sequence, expression of catalytic activity and comparison with the human enzyme."
      Nanba E., Suzuki K.
      Biochem. Biophys. Res. Commun. 173:141-148(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Tissue: Brain.
    2. "Organization of the mouse acid beta-galactosidase gene."
      Nanba E., Suzuki K.
      Biochem. Biophys. Res. Commun. 178:158-164(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DBA/2J.

    Entry informationi

    Entry nameiBGAL_MOUSE
    AccessioniPrimary (citable) accession number: P23780
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3