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Protein

Beta-galactosidase

Gene

Glb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei189Proton donorSequence analysis1
Active sitei269NucleophileSequence analysis1

GO - Molecular functioni

  • beta-galactosidase activity Source: MGI
  • galactoside binding Source: Ensembl
  • hydrolase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.
R-MMU-2022857. Keratan sulfate degradation.
R-MMU-2024096. HS-GAG degradation.
R-MMU-4085001. Sialic acid metabolism.
R-MMU-6798695. Neutrophil degranulation.
SABIO-RKP23780.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Alternative name(s):
Acid beta-galactosidase
Short name:
Lactase
Gene namesi
Name:Glb1
Synonyms:Bgl, Glb-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88151. Glb1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1667667.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000001219025 – 29By similarity5
ChainiPRO_000001219130 – 647Beta-galactosidaseAdd BLAST618

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (GlcNAc...)Sequence analysis1
Glycosylationi248N-linked (GlcNAc...)Sequence analysis1
Glycosylationi500N-linked (GlcNAc...)Sequence analysis1
Glycosylationi504N-linked (GlcNAc...)Sequence analysis1
Glycosylationi510N-linked (GlcNAc...)Sequence analysis1
Glycosylationi544N-linked (GlcNAc...)Sequence analysis1
Glycosylationi557N-linked (GlcNAc...)Sequence analysis1
Glycosylationi617N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Zymogen

Proteomic databases

EPDiP23780.
PaxDbiP23780.
PeptideAtlasiP23780.
PRIDEiP23780.

PTM databases

iPTMnetiP23780.
PhosphoSitePlusiP23780.
SwissPalmiP23780.

Expressioni

Gene expression databases

BgeeiENSMUSG00000045594.
CleanExiMM_GLB1.
ExpressionAtlasiP23780. baseline and differential.
GenevisibleiP23780. MM.

Interactioni

Protein-protein interaction databases

BioGridi198341. 1 interactor.
IntActiP23780. 1 interactor.
MINTiMINT-4089206.
STRINGi10090.ENSMUSP00000055803.

Chemistry databases

BindingDBiP23780.

Structurei

3D structure databases

ProteinModelPortaliP23780.
SMRiP23780.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0496. Eukaryota.
COG1874. LUCA.
GeneTreeiENSGT00390000006586.
HOGENOMiHOG000221607.
HOVERGENiHBG004841.
InParanoidiP23780.
KOiK12309.
OMAiENMGRVS.
OrthoDBiEOG091G006X.
PhylomeDBiP23780.
TreeFamiTF314816.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR026283. B-gal_1-like.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF13364. BetaGal_dom4_5. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFiPIRSF006336. B-gal. 1 hit.
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRVPLCTPL PLLALLQLLG AAHGIYNVTQ RTFKLDYSRD RFLKDGQPFR
60 70 80 90 100
YISGSIHYFR IPRFYWEDRL LKMKMAGLNA IQMYVPWNFH EPQPGQYEFS
110 120 130 140 150
GDRDVEHFIQ LAHELGLLVI LRPGPYICAE WDMGGLPAWL LEKQSIVLRS
160 170 180 190 200
SDPDYLVAVD KWLAVLLPKM KPLLYQNGGP IITVQVENEY GSYFACDYDY
210 220 230 240 250
LRFLVHRFRY HLGNDVILFT TDGASEKMLK CGTLQDLYAT VDFGTGNNIT
260 270 280 290 300
QAFLVQRKFE PKGPLINSEF YTGWLDHWGK PHSTVKTKTL ATSLYNLLAR
310 320 330 340 350
GANVNLYMFI GGTNFAYWNG ANTPYEPQPT SYDYDAPLSE AGDLTKKYFA
360 370 380 390 400
LREVIQMFKE VPEGPIPPST PKFAYGKVAL RKFKTVAEAL GILCPNGPVK
410 420 430 440 450
SLYPLTFTQV KQYFGYVLYR TTLPQDCSNP KPIFSSPFNG VRDRAYVSVD
460 470 480 490 500
GVPQGILDRN LMTALNIRGK AGATLDILVE NMGRVNYGRF INDFKGLISN
510 520 530 540 550
MTINSTVLTN WTVFPLNTEA MVRNHLWGRE ASDEGHLDGR STSNSSDLIL
560 570 580 590 600
PTFYVGNFSI PSGIPDLPQD TFIQFPGWSK GQVWINGFNL GRYWPTMGPQ
610 620 630 640
KTLFVPRNIL TTSAPNNITV LELEFAPCSE GTPELCTVEF VDTPVIS
Length:647
Mass (Da):73,121
Last modified:November 1, 1991 - v1
Checksum:i0E68EAA66A10803A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti517N → D in AAA37292 (PubMed:1906271).Curated1
Sequence conflicti539G → R in AAA37292 (PubMed:1906271).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57734 mRNA. Translation: AAA37293.1.
M75122
, M75137, M75107, M75108, M75109, M75111, M75112, M75113, M75114, M75115, M75116, M75117, M75118, M75119, M75120, M75121 Genomic DNA. Translation: AAA37292.1.
CCDSiCCDS23593.1.
PIRiA37086.
RefSeqiNP_033882.1. NM_009752.2.
UniGeneiMm.290516.

Genome annotation databases

EnsembliENSMUST00000063042; ENSMUSP00000055803; ENSMUSG00000045594.
GeneIDi12091.
KEGGimmu:12091.
UCSCiuc009rxj.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57734 mRNA. Translation: AAA37293.1.
M75122
, M75137, M75107, M75108, M75109, M75111, M75112, M75113, M75114, M75115, M75116, M75117, M75118, M75119, M75120, M75121 Genomic DNA. Translation: AAA37292.1.
CCDSiCCDS23593.1.
PIRiA37086.
RefSeqiNP_033882.1. NM_009752.2.
UniGeneiMm.290516.

3D structure databases

ProteinModelPortaliP23780.
SMRiP23780.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198341. 1 interactor.
IntActiP23780. 1 interactor.
MINTiMINT-4089206.
STRINGi10090.ENSMUSP00000055803.

Chemistry databases

BindingDBiP23780.
ChEMBLiCHEMBL1667667.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

PTM databases

iPTMnetiP23780.
PhosphoSitePlusiP23780.
SwissPalmiP23780.

Proteomic databases

EPDiP23780.
PaxDbiP23780.
PeptideAtlasiP23780.
PRIDEiP23780.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063042; ENSMUSP00000055803; ENSMUSG00000045594.
GeneIDi12091.
KEGGimmu:12091.
UCSCiuc009rxj.3. mouse.

Organism-specific databases

CTDi2720.
MGIiMGI:88151. Glb1.

Phylogenomic databases

eggNOGiKOG0496. Eukaryota.
COG1874. LUCA.
GeneTreeiENSGT00390000006586.
HOGENOMiHOG000221607.
HOVERGENiHBG004841.
InParanoidiP23780.
KOiK12309.
OMAiENMGRVS.
OrthoDBiEOG091G006X.
PhylomeDBiP23780.
TreeFamiTF314816.

Enzyme and pathway databases

ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.
R-MMU-2022857. Keratan sulfate degradation.
R-MMU-2024096. HS-GAG degradation.
R-MMU-4085001. Sialic acid metabolism.
R-MMU-6798695. Neutrophil degranulation.
SABIO-RKP23780.

Miscellaneous databases

PROiP23780.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045594.
CleanExiMM_GLB1.
ExpressionAtlasiP23780. baseline and differential.
GenevisibleiP23780. MM.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR026283. B-gal_1-like.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF13364. BetaGal_dom4_5. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFiPIRSF006336. B-gal. 1 hit.
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_MOUSE
AccessioniPrimary (citable) accession number: P23780
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.