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Protein

Beta-galactosidase

Gene

Glb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84SubstrateBy similarity1
Binding sitei130SubstrateBy similarity1
Binding sitei188SubstrateBy similarity1
Active sitei189Proton donorBy similarity1
Active sitei269NucleophileBy similarity1
Binding sitei334SubstrateBy similarity1

GO - Molecular functioni

  • beta-galactosidase activity Source: MGI
  • galactoside binding Source: MGI
  • hydrolase activity Source: MGI
  • protein homodimerization activity Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiR-MMU-1660662 Glycosphingolipid metabolism
R-MMU-2022857 Keratan sulfate degradation
R-MMU-2024096 HS-GAG degradation
R-MMU-4085001 Sialic acid metabolism
R-MMU-6798695 Neutrophil degranulation
SABIO-RKiP23780

Protein family/group databases

CAZyiGH35 Glycoside Hydrolase Family 35

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.231 Publication)
Alternative name(s):
Acid beta-galactosidase
Short name:
Lactase
Gene namesi
Name:Glb1
Synonyms:Bgl, Glb-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88151 Glb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1667667

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000001219025 – 29By similarity5
ChainiPRO_000001219130 – 647Beta-galactosidaseAdd BLAST618

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi196 ↔ 231By similarity
Glycosylationi248N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi500N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi504N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi510N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi544N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi557N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi617N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi628 ↔ 636By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP23780
PaxDbiP23780
PeptideAtlasiP23780
PRIDEiP23780

PTM databases

iPTMnetiP23780
PhosphoSitePlusiP23780
SwissPalmiP23780

Expressioni

Gene expression databases

BgeeiENSMUSG00000045594
CleanExiMM_GLB1
ExpressionAtlasiP23780 baseline and differential
GenevisibleiP23780 MM

Interactioni

Subunit structurei

Homodimer. May form higher multimers.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198341, 1 interactor
IntActiP23780, 1 interactor
MINTiP23780
STRINGi10090.ENSMUSP00000055803

Chemistry databases

BindingDBiP23780

Structurei

3D structure databases

ProteinModelPortaliP23780
SMRiP23780
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0496 Eukaryota
COG1874 LUCA
GeneTreeiENSGT00390000006586
HOGENOMiHOG000221607
HOVERGENiHBG004841
InParanoidiP23780
KOiK12309
OMAiGWGKGIV
OrthoDBiEOG091G006X
PhylomeDBiP23780
TreeFamiTF314816

Family and domain databases

Gene3Di2.60.120.260, 3 hits
InterProiView protein in InterPro
IPR026283 B-gal_1-like
IPR025300 BetaGal_jelly_roll_dom
IPR008979 Galactose-bd-like_sf
IPR031330 Gly_Hdrlase_35_cat
IPR019801 Glyco_hydro_35_CS
IPR001944 Glycoside_Hdrlase_35
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR23421 PTHR23421, 1 hit
PfamiView protein in Pfam
PF13364 BetaGal_dom4_5, 1 hit
PF01301 Glyco_hydro_35, 1 hit
PIRSFiPIRSF006336 B-gal, 1 hit
PRINTSiPR00742 GLHYDRLASE35
SUPFAMiSSF49785 SSF49785, 2 hits
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS01182 GLYCOSYL_HYDROL_F35, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRVPLCTPL PLLALLQLLG AAHGIYNVTQ RTFKLDYSRD RFLKDGQPFR
60 70 80 90 100
YISGSIHYFR IPRFYWEDRL LKMKMAGLNA IQMYVPWNFH EPQPGQYEFS
110 120 130 140 150
GDRDVEHFIQ LAHELGLLVI LRPGPYICAE WDMGGLPAWL LEKQSIVLRS
160 170 180 190 200
SDPDYLVAVD KWLAVLLPKM KPLLYQNGGP IITVQVENEY GSYFACDYDY
210 220 230 240 250
LRFLVHRFRY HLGNDVILFT TDGASEKMLK CGTLQDLYAT VDFGTGNNIT
260 270 280 290 300
QAFLVQRKFE PKGPLINSEF YTGWLDHWGK PHSTVKTKTL ATSLYNLLAR
310 320 330 340 350
GANVNLYMFI GGTNFAYWNG ANTPYEPQPT SYDYDAPLSE AGDLTKKYFA
360 370 380 390 400
LREVIQMFKE VPEGPIPPST PKFAYGKVAL RKFKTVAEAL GILCPNGPVK
410 420 430 440 450
SLYPLTFTQV KQYFGYVLYR TTLPQDCSNP KPIFSSPFNG VRDRAYVSVD
460 470 480 490 500
GVPQGILDRN LMTALNIRGK AGATLDILVE NMGRVNYGRF INDFKGLISN
510 520 530 540 550
MTINSTVLTN WTVFPLNTEA MVRNHLWGRE ASDEGHLDGR STSNSSDLIL
560 570 580 590 600
PTFYVGNFSI PSGIPDLPQD TFIQFPGWSK GQVWINGFNL GRYWPTMGPQ
610 620 630 640
KTLFVPRNIL TTSAPNNITV LELEFAPCSE GTPELCTVEF VDTPVIS
Length:647
Mass (Da):73,121
Last modified:November 1, 1991 - v1
Checksum:i0E68EAA66A10803A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti517N → D in AAA37292 (PubMed:1906271).Curated1
Sequence conflicti539G → R in AAA37292 (PubMed:1906271).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57734 mRNA Translation: AAA37293.1
M75122
, M75137, M75107, M75108, M75109, M75111, M75112, M75113, M75114, M75115, M75116, M75117, M75118, M75119, M75120, M75121 Genomic DNA Translation: AAA37292.1
CCDSiCCDS23593.1
PIRiA37086
RefSeqiNP_033882.1, NM_009752.2
UniGeneiMm.290516

Genome annotation databases

EnsembliENSMUST00000063042; ENSMUSP00000055803; ENSMUSG00000045594
GeneIDi12091
KEGGimmu:12091
UCSCiuc009rxj.3 mouse

Similar proteinsi

Entry informationi

Entry nameiBGAL_MOUSE
AccessioniPrimary (citable) accession number: P23780
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 20, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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