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Reviewed, UniProtKB/Swiss-Prot P23780 (BGAL_MOUSE)

Last modified November 3, 2009. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Beta-galactosidase
    EC=3.2.1.23
Alternative name(s):
    Acid beta-galactosidase
      Short name=Lactase
Gene names
Name: Glb1
Synonyms: Bgl, Glb-1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length647 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Lysosome.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

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Ontologies

Keywords
   Cellular componentLysosome
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Zymogen
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionbeta-galactosidase activity

Inferred from direct assay. Source: MGI

cation binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 295 By similarity
PRO_0000012190
Chain30 – 647618Beta-galactosidase
PRO_0000012191

Sites

Active site1891Proton donor Potential
Active site2691Nucleophile Potential

Amino acid modifications

Glycosylation271N-linked (GlcNAc...) Potential
Glycosylation2481N-linked (GlcNAc...) Potential
Glycosylation5001N-linked (GlcNAc...) Potential
Glycosylation5041N-linked (GlcNAc...) Potential
Glycosylation5101N-linked (GlcNAc...) Potential
Glycosylation5441N-linked (GlcNAc...) Potential
Glycosylation5571N-linked (GlcNAc...) Potential
Glycosylation6171N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict5171N → D in AAA37292. Ref.2
Sequence conflict5391G → R in AAA37292. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
P23780-1 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 0E68EAA66A10803A

FASTA64773,121
        10         20         30         40         50         60 
MLRVPLCTPL PLLALLQLLG AAHGIYNVTQ RTFKLDYSRD RFLKDGQPFR YISGSIHYFR 

        70         80         90        100        110        120 
IPRFYWEDRL LKMKMAGLNA IQMYVPWNFH EPQPGQYEFS GDRDVEHFIQ LAHELGLLVI 

       130        140        150        160        170        180 
LRPGPYICAE WDMGGLPAWL LEKQSIVLRS SDPDYLVAVD KWLAVLLPKM KPLLYQNGGP 

       190        200        210        220        230        240 
IITVQVENEY GSYFACDYDY LRFLVHRFRY HLGNDVILFT TDGASEKMLK CGTLQDLYAT 

       250        260        270        280        290        300 
VDFGTGNNIT QAFLVQRKFE PKGPLINSEF YTGWLDHWGK PHSTVKTKTL ATSLYNLLAR 

       310        320        330        340        350        360 
GANVNLYMFI GGTNFAYWNG ANTPYEPQPT SYDYDAPLSE AGDLTKKYFA LREVIQMFKE 

       370        380        390        400        410        420 
VPEGPIPPST PKFAYGKVAL RKFKTVAEAL GILCPNGPVK SLYPLTFTQV KQYFGYVLYR 

       430        440        450        460        470        480 
TTLPQDCSNP KPIFSSPFNG VRDRAYVSVD GVPQGILDRN LMTALNIRGK AGATLDILVE 

       490        500        510        520        530        540 
NMGRVNYGRF INDFKGLISN MTINSTVLTN WTVFPLNTEA MVRNHLWGRE ASDEGHLDGR 

       550        560        570        580        590        600 
STSNSSDLIL PTFYVGNFSI PSGIPDLPQD TFIQFPGWSK GQVWINGFNL GRYWPTMGPQ 

       610        620        630        640 
KTLFVPRNIL TTSAPNNITV LELEFAPCSE GTPELCTVEF VDTPVIS

« Hide

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References

[1]"Molecular cloning of mouse acid beta-galactosidase cDNA: sequence, expression of catalytic activity and comparison with the human enzyme."
Nanba E., Suzuki K.
Biochem. Biophys. Res. Commun. 173:141-148(1990) [PubMed: 2124109] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Brain.
[2]"Organization of the mouse acid beta-galactosidase gene."
Nanba E., Suzuki K.
Biochem. Biophys. Res. Commun. 178:158-164(1991) [PubMed: 1906271] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DBA/2J.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M57734 mRNA. Translation: AAA37293.1.
M75122 expand/collapse EMBL AC list , M75137, M75107, M75108, M75109, M75111, M75112, M75113, M75114, M75115, M75116, M75117, M75118, M75119, M75120, M75121 Genomic DNA. Translation: AAA37292.1.
IPIIPI00135915.
PIRA37086.
RefSeqNP_033882.1.
UniGeneMm.290516
Mm.440489

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGP23780.

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

PTM databases

PhosphoSiteP23780.

Proteomic databases

PRIDEP23780.

Genome annotation databases

EnsemblENSMUST00000063042; ENSMUSP00000055803; ENSMUSG00000045594; Mus musculus. [Genome view]
GeneID12091.
KEGGmmu:12091.
UCSCuc009rxm.1. mouse.

Organism-specific databases

CTD12091.
MGIMGI:88151. Glb1.

Phylogenomic databases

HOVERGENP23780.
OMAFAYGKVA.

Enzyme and pathway databases

BRENDA3.2.1.23. 244.

Gene expression databases

ArrayExpressP23780.
BgeeP23780.
CleanExMM_GLB1.
GenevestigatorP23780.
GermOnlineENSMUSG00000045594. Mus musculus.

Family and domain databases

InterProIPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_sg_catalytic.
IPR001944. Glycoside_Hdrlase_35.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHERPTHR23421. Glyco_hydro_35. 1 hit.
PfamPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio280407.
SOURCESearch...

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Entry information

Entry nameBGAL_MOUSE
AccessionPrimary (citable) accession number: P23780
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 3, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents