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P23780 (BGAL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-galactosidase
EC=3.2.1.23
Alternative name(s):
Acid beta-galactosidase
Short name=Lactase
Gene names
Name:Glb1
Synonyms:Bgl, Glb-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length647 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Lysosome.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
   Cellular componentLysosome
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processgalactose catabolic process

Inferred from electronic annotation. Source: Compara

   Cellular_componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from direct assay PubMed 1914521PubMed 4830244PubMed 7203014. Source: MGI

galactoside binding

Inferred from electronic annotation. Source: Compara

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 295 By similarity
PRO_0000012190
Chain30 – 647618Beta-galactosidase
PRO_0000012191

Sites

Active site1891Proton donor Potential
Active site2691Nucleophile Potential

Amino acid modifications

Glycosylation271N-linked (GlcNAc...) Potential
Glycosylation2481N-linked (GlcNAc...) Potential
Glycosylation5001N-linked (GlcNAc...) Potential
Glycosylation5041N-linked (GlcNAc...) Potential
Glycosylation5101N-linked (GlcNAc...) Potential
Glycosylation5441N-linked (GlcNAc...) Potential
Glycosylation5571N-linked (GlcNAc...) Potential
Glycosylation6171N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict5171N → D in AAA37292. Ref.2
Sequence conflict5391G → R in AAA37292. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P23780 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 0E68EAA66A10803A

FASTA64773,121
        10         20         30         40         50         60 
MLRVPLCTPL PLLALLQLLG AAHGIYNVTQ RTFKLDYSRD RFLKDGQPFR YISGSIHYFR 

        70         80         90        100        110        120 
IPRFYWEDRL LKMKMAGLNA IQMYVPWNFH EPQPGQYEFS GDRDVEHFIQ LAHELGLLVI 

       130        140        150        160        170        180 
LRPGPYICAE WDMGGLPAWL LEKQSIVLRS SDPDYLVAVD KWLAVLLPKM KPLLYQNGGP 

       190        200        210        220        230        240 
IITVQVENEY GSYFACDYDY LRFLVHRFRY HLGNDVILFT TDGASEKMLK CGTLQDLYAT 

       250        260        270        280        290        300 
VDFGTGNNIT QAFLVQRKFE PKGPLINSEF YTGWLDHWGK PHSTVKTKTL ATSLYNLLAR 

       310        320        330        340        350        360 
GANVNLYMFI GGTNFAYWNG ANTPYEPQPT SYDYDAPLSE AGDLTKKYFA LREVIQMFKE 

       370        380        390        400        410        420 
VPEGPIPPST PKFAYGKVAL RKFKTVAEAL GILCPNGPVK SLYPLTFTQV KQYFGYVLYR 

       430        440        450        460        470        480 
TTLPQDCSNP KPIFSSPFNG VRDRAYVSVD GVPQGILDRN LMTALNIRGK AGATLDILVE 

       490        500        510        520        530        540 
NMGRVNYGRF INDFKGLISN MTINSTVLTN WTVFPLNTEA MVRNHLWGRE ASDEGHLDGR 

       550        560        570        580        590        600 
STSNSSDLIL PTFYVGNFSI PSGIPDLPQD TFIQFPGWSK GQVWINGFNL GRYWPTMGPQ 

       610        620        630        640 
KTLFVPRNIL TTSAPNNITV LELEFAPCSE GTPELCTVEF VDTPVIS

« Hide

References

[1]"Molecular cloning of mouse acid beta-galactosidase cDNA: sequence, expression of catalytic activity and comparison with the human enzyme."
Nanba E., Suzuki K.
Biochem. Biophys. Res. Commun. 173:141-148(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Brain.
[2]"Organization of the mouse acid beta-galactosidase gene."
Nanba E., Suzuki K.
Biochem. Biophys. Res. Commun. 178:158-164(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DBA/2J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M57734 mRNA. Translation: AAA37293.1.
M75122 expand/collapse EMBL AC list , M75137, M75107, M75108, M75109, M75111, M75112, M75113, M75114, M75115, M75116, M75117, M75118, M75119, M75120, M75121 Genomic DNA. Translation: AAA37292.1.
IPIIPI00135915.
PIRA37086.
RefSeqNP_033882.1. NM_009752.1.
UniGeneMm.290516.

3D structure databases

ProteinModelPortalP23780.
SMRP23780. Positions 30-646.
ModBaseSearch...

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

PTM databases

PhosphoSiteP23780.

Proteomic databases

PaxDbP23780.
PRIDEP23780.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000063042; ENSMUSP00000055803; ENSMUSG00000045594.
GeneID12091.
KEGGmmu:12091.

Organism-specific databases

CTD2720.
MGIMGI:88151. Glb1.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000221607.
HOVERGENHBG004841.
InParanoidP23780.
KOK12309.
OMARIPRVYW.
OrthoDBEOG46MBJ7.

Gene expression databases

ArrayExpressP23780.
BgeeP23780.
CleanExMM_GLB1.
GenevestigatorP23780.
GermOnlineENSMUSG00000045594. Mus musculus.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR026283. B-gal_1-like.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFPIRSF006336. B-gal. 1 hit.
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. Gal_bind_like. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP23780.
ChEMBLCHEMBL1667667.
NextBio280407.
SOURCESearch...

Entry information

Entry nameBGAL_MOUSE
AccessionPrimary (citable) accession number: P23780
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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