P23778 (PP2A_BRANA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Serine/threonine-protein phosphatase PP2A catalytic subunit EC=3.1.3.16
Organism	Brassica napus (Rape)
Taxonomic identifier	3708 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	309 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-2A subfamily.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 309	›309	Serine/threonine-protein phosphatase PP2A catalytic subunit		PRO_0000058857
Sites
	Active site	118	1	Proton donor By similarity
	Metal binding	57	1	Iron By similarity
	Metal binding	59	1	Iron By similarity
	Metal binding	85	1	Iron By similarity
	Metal binding	85	1	Manganese By similarity
	Metal binding	117	1	Manganese By similarity
	Metal binding	167	1	Manganese By similarity
	Metal binding	241	1	Manganese By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P23778 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 50701E2E6768F581	FASTA	309	35,176
	10 20 30 40 50 60 SIPTDATLDL DEQISQLMQC KPLSEQQVRA LCEKAKEILM DESNVQPVKS PVTICGDIHG 70 80 90 100 110 120 QFHDLAELFR IGGMCPDTNY LFMGDYVDRG YYSVETVTLL VGLKVRYPQR ITILRGNHES 130 140 150 160 170 180 RQITQVYGFY DECLRKYGNA NVWKYFTDLF DYLPLTALVE SEIFCLHGGL SPSIETLDNI 190 200 210 220 230 240 RNFDRVQEVP HGGPMCDLLW SDPDDRCGWG ISPRGAGYTF GQDISNQFNH SNSLKLISRA 250 260 270 280 290 300 HQLVMDGYNW AHEAKGGTIF SAPNYCYRCG NMASILEVDD CRNHTFIQFE PAPRRGEPDV TRRTPDYFL

References

[1]

"Identification by molecular cloning of two cDNA sequences from the plant Brassica napus which are very similar to mammalian protein phosphatases-1 and -2A." Mackintosh R.W., Haycox G., Hardie D.G., Cohen P.T.W. FEBS Lett. 276:156-160(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57439 mRNA. Translation: CAA40687.1.
PIR	S12986.
3D structure databases
ProteinModelPortal	P23778.
SMR	P23778. Positions 7-309.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP2A_BRANA

Accession

Primary (citable) accession number: P23778

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program