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Protein

Trans-acting T-cell-specific transcription factor GATA-3

Gene

Gata3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri263 – 287GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri317 – 341GATA-type 2PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • anatomical structure formation involved in morphogenesis Source: MGI
  • aortic valve morphogenesis Source: UniProtKB
  • axon guidance Source: MGI
  • canonical Wnt signaling pathway involved in metanephric kidney development Source: UniProtKB
  • cardiac right ventricle morphogenesis Source: UniProtKB
  • cell activation Source: MGI
  • cell fate determination Source: MGI
  • cell maturation Source: MGI
  • cell morphogenesis Source: MGI
  • cellular response to BMP stimulus Source: MGI
  • cellular response to cytokine stimulus Source: MGI
  • cellular response to interferon-alpha Source: Ensembl
  • cellular response to interleukin-4 Source: UniProtKB
  • cellular response to tumor necrosis factor Source: Ensembl
  • chromatin remodeling Source: MGI
  • cochlea development Source: Ensembl
  • developmental growth Source: MGI
  • digestive tract development Source: GO_Central
  • ear development Source: UniProtKB
  • embryonic hemopoiesis Source: MGI
  • embryonic organ development Source: MGI
  • erythrocyte differentiation Source: MGI
  • homeostasis of number of cells Source: MGI
  • humoral immune response Source: MGI
  • innate immune response Source: UniProtKB-KW
  • inner ear morphogenesis Source: MGI
  • interferon-gamma secretion Source: MGI
  • interleukin-4 secretion Source: MGI
  • in utero embryonic development Source: MGI
  • kidney development Source: MGI
  • lens development in camera-type eye Source: MGI
  • lymphocyte migration Source: UniProtKB
  • male gonad development Source: UniProtKB
  • mast cell differentiation Source: MGI
  • mesenchymal to epithelial transition Source: UniProtKB
  • mesonephros development Source: MGI
  • negative regulation of cell cycle Source: UniProtKB
  • negative regulation of cell motility Source: UniProtKB
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of cell proliferation involved in mesonephros development Source: UniProtKB
  • negative regulation of DNA demethylation Source: MGI
  • negative regulation of endothelial cell apoptotic process Source: UniProtKB
  • negative regulation of fat cell differentiation Source: UniProtKB
  • negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation Source: UniProtKB
  • negative regulation of gene expression Source: MGI
  • negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation Source: UniProtKB
  • negative regulation of inflammatory response Source: UniProtKB
  • negative regulation of interferon-gamma production Source: MGI
  • negative regulation of interleukin-2 production Source: MGI
  • negative regulation of mammary gland epithelial cell proliferation Source: MGI
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • nephric duct formation Source: UniProtKB
  • nephric duct morphogenesis Source: UniProtKB
  • nervous system development Source: MGI
  • neuron differentiation Source: MGI
  • neuron migration Source: MGI
  • norepinephrine biosynthetic process Source: MGI
  • otic vesicle development Source: MGI
  • parathyroid gland development Source: MGI
  • parathyroid hormone secretion Source: MGI
  • pharyngeal system development Source: UniProtKB
  • phosphatidylinositol 3-kinase signaling Source: UniProtKB
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of cytokine production Source: MGI
  • positive regulation of endothelial cell migration Source: UniProtKB
  • positive regulation of gene expression Source: MGI
  • positive regulation of histone H3-K14 acetylation Source: MGI
  • positive regulation of histone H3-K9 acetylation Source: MGI
  • positive regulation of interleukin-13 production Source: MGI
  • positive regulation of interleukin-13 secretion Source: UniProtKB
  • positive regulation of interleukin-4 production Source: MGI
  • positive regulation of interleukin-5 production Source: MGI
  • positive regulation of interleukin-5 secretion Source: UniProtKB
  • positive regulation of protein kinase B signaling Source: UniProtKB
  • positive regulation of signal transduction Source: UniProtKB
  • positive regulation of T cell differentiation Source: UniProtKB
  • positive regulation of T-helper 2 cell cytokine production Source: MGI
  • positive regulation of thyroid hormone generation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of transcription regulatory region DNA binding Source: UniProtKB
  • positive regulation of ureteric bud formation Source: UniProtKB
  • post-embryonic development Source: MGI
  • pro-T cell differentiation Source: MGI
  • regulation of CD4-positive, alpha-beta T cell differentiation Source: MGI
  • regulation of cellular response to X-ray Source: UniProtKB
  • regulation of cytokine biosynthetic process Source: MGI
  • regulation of establishment of cell polarity Source: MGI
  • regulation of histone H3-K27 methylation Source: MGI
  • regulation of histone H3-K4 methylation Source: MGI
  • regulation of nephron tubule epithelial cell differentiation Source: UniProtKB
  • regulation of neuron apoptotic process Source: MGI
  • regulation of neuron projection development Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • renal system development Source: MGI
  • response to drug Source: Ensembl
  • response to estrogen Source: Ensembl
  • response to ethanol Source: Ensembl
  • response to gamma radiation Source: Ensembl
  • response to virus Source: Ensembl
  • signal transduction Source: UniProtKB
  • sympathetic nervous system development Source: MGI
  • T cell differentiation Source: MGI
  • T cell differentiation in thymus Source: MGI
  • T cell receptor signaling pathway Source: UniProtKB
  • T-helper 2 cell differentiation Source: MGI
  • thymic T cell selection Source: MGI
  • thymus development Source: MGI
  • TOR signaling Source: UniProtKB
  • type IV hypersensitivity Source: Ensembl
  • ureteric bud formation Source: UniProtKB
  • ureter maturation Source: MGI
  • uterus development Source: UniProtKB
  • ventricular septum development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Immunity, Innate immunity, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5689880. Ub-specific processing proteases.
R-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-acting T-cell-specific transcription factor GATA-3
Alternative name(s):
GATA-binding factor 3
Gene namesi
Name:Gata3
Synonyms:Gata-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95663. Gata3.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromatin Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: UniProtKB
  • transcription factor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi344Y → A: Dramatically decreased Th2 cell differentiation. 1 Publication1
Mutagenesisi346K → A: Moderately decreased Th2 cell differentiation. 1 Publication1
Mutagenesisi348H → A: Dramatically decreased Th2 cell differentiation. 1 Publication1
Mutagenesisi352R → A: Fails to induce Th2 cytokine production. 1 Publication1
Mutagenesisi353P → A or K: Fails to induce Th2 cytokine production. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834091 – 443Trans-acting T-cell-specific transcription factor GATA-3Add BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei114PhosphoserineBy similarity1
Modified residuei161PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP23772.
PRIDEiP23772.

PTM databases

iPTMnetiP23772.
PhosphoSitePlusiP23772.

Expressioni

Tissue specificityi

T-cell specific.

Gene expression databases

BgeeiENSMUSG00000015619.
CleanExiMM_GATA3.
ExpressionAtlasiP23772. baseline and differential.
GenevisibleiP23772. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199840. 4 interactors.
DIPiDIP-29712N.
IntActiP23772. 3 interactors.
STRINGi10090.ENSMUSP00000100041.

Structurei

Secondary structure

1443
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni318 – 320Combined sources3
Beta strandi326 – 330Combined sources5
Beta strandi336 – 338Combined sources3
Helixi339 – 348Combined sources10
Helixi354 – 356Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DFVX-ray3.10C/D308-370[»]
3DFXX-ray2.70A/B308-370[»]
ProteinModelPortaliP23772.
SMRiP23772.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23772.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni288 – 316Flexible linkerBy similarityAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi344 – 353YxKxHxxxRP10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi136 – 139Poly-Ser4
Compositional biasi322 – 326Poly-Thr5

Domaini

Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation (By similarity).By similarity
The YxKxHxxxRP motif is critical for DNA-binding and function.1 Publication

Sequence similaritiesi

Contains 2 GATA-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri263 – 287GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri317 – 341GATA-type 2PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000047701.
HOVERGENiHBG051705.
InParanoidiP23772.
KOiK17895.
OMAiQRYPPTH.
OrthoDBiEOG091G0AUR.
PhylomeDBiP23772.
TreeFamiTF315391.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR029521. GATA-3.
IPR016374. TF_GATA-2/3.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF106. PTHR10071:SF106. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PIRSFiPIRSF003027. TF_GATA-1/2/3. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LGHSYMEAQY PLTEEVDVLF
60 70 80 90 100
NIDGQGNHVP SYYGNSVRAT VQRYPPTHHG SQVCRPPLLH GSLPWLDGGK
110 120 130 140 150
ALSSHHTASP WNLSPFSKTS IHHGSPGPLS VYPPASSSSL AAGHSSPHLF
160 170 180 190 200
TFPPTPPKDV SPDPSLSTPG SAGSARQDEK ECLKYQVQLP DSMKLETSHS
210 220 230 240 250
RGSMTTLGGA SSSAHHPITT YPPYVPEYSS GLFPPSSLLG GSPTGFGCKS
260 270 280 290 300
RPKARSSTEG RECVNCGATS TPLWRRDGTG HYLCNACGLY HKMNGQNRPL
310 320 330 340 350
IKPKRRLSAA RRAGTSCANC QTTTTTLWRR NANGDPVCNA CGLYYKLHNI
360 370 380 390 400
NRPLTMKKEG IQTRNRKMSS KSKKCKKVHD ALEDFPKSSS FNPAALSRHM
410 420 430 440
SSLSHISPFS HSSHMLTTPT PMHPPSGLSF GPHHPSSMVT AMG
Length:443
Mass (Da):47,968
Last modified:November 1, 1991 - v1
Checksum:i980E58FB9872E560
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55123 mRNA. Translation: CAA38917.1.
BC062915 mRNA. Translation: AAH62915.1.
CCDSiCCDS15674.1.
PIRiB39794.
RefSeqiNP_032117.1. NM_008091.3.
XP_006497417.1. XM_006497354.1.
UniGeneiMm.313866.

Genome annotation databases

EnsembliENSMUST00000102976; ENSMUSP00000100041; ENSMUSG00000015619.
GeneIDi14462.
KEGGimmu:14462.
UCSCiuc008ihf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55123 mRNA. Translation: CAA38917.1.
BC062915 mRNA. Translation: AAH62915.1.
CCDSiCCDS15674.1.
PIRiB39794.
RefSeqiNP_032117.1. NM_008091.3.
XP_006497417.1. XM_006497354.1.
UniGeneiMm.313866.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DFVX-ray3.10C/D308-370[»]
3DFXX-ray2.70A/B308-370[»]
ProteinModelPortaliP23772.
SMRiP23772.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199840. 4 interactors.
DIPiDIP-29712N.
IntActiP23772. 3 interactors.
STRINGi10090.ENSMUSP00000100041.

PTM databases

iPTMnetiP23772.
PhosphoSitePlusiP23772.

Proteomic databases

PaxDbiP23772.
PRIDEiP23772.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102976; ENSMUSP00000100041; ENSMUSG00000015619.
GeneIDi14462.
KEGGimmu:14462.
UCSCiuc008ihf.1. mouse.

Organism-specific databases

CTDi2625.
MGIiMGI:95663. Gata3.

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000047701.
HOVERGENiHBG051705.
InParanoidiP23772.
KOiK17895.
OMAiQRYPPTH.
OrthoDBiEOG091G0AUR.
PhylomeDBiP23772.
TreeFamiTF315391.

Enzyme and pathway databases

ReactomeiR-MMU-5689880. Ub-specific processing proteases.
R-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

EvolutionaryTraceiP23772.
PROiP23772.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015619.
CleanExiMM_GATA3.
ExpressionAtlasiP23772. baseline and differential.
GenevisibleiP23772. MM.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR029521. GATA-3.
IPR016374. TF_GATA-2/3.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF106. PTHR10071:SF106. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PIRSFiPIRSF003027. TF_GATA-1/2/3. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA3_MOUSE
AccessioniPrimary (citable) accession number: P23772
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 2, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.