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P23772

- GATA3_MOUSE

UniProt

P23772 - GATA3_MOUSE

Protein

Trans-acting T-cell-specific transcription factor GATA-3

Gene

Gata3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri263 – 28725GATA-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri317 – 34125GATA-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. core promoter proximal region sequence-specific DNA binding Source: UniProtKB
    2. E-box binding Source: UniProtKB
    3. enhancer sequence-specific DNA binding Source: UniProtKB
    4. interleukin-2 receptor binding Source: MGI
    5. protein binding Source: UniProtKB
    6. protein dimerization activity Source: MGI
    7. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: UniProtKB
    8. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
    9. RNA polymerase II core promoter sequence-specific DNA binding Source: MGI
    10. RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
    11. sequence-specific DNA binding Source: MGI
    12. sequence-specific DNA binding transcription factor activity Source: UniProtKB
    13. transcription coactivator activity Source: UniProtKB
    14. transcription regulatory region DNA binding Source: UniProtKB
    15. zinc ion binding Source: InterPro

    GO - Biological processi

    1. anatomical structure formation involved in morphogenesis Source: MGI
    2. aortic valve morphogenesis Source: UniProtKB
    3. axon guidance Source: MGI
    4. canonical Wnt signaling pathway involved in metanephric kidney development Source: UniProtKB
    5. cardiac right ventricle morphogenesis Source: UniProtKB
    6. cell activation Source: MGI
    7. cell fate determination Source: MGI
    8. cell maturation Source: MGI
    9. cell morphogenesis Source: MGI
    10. cellular response to BMP stimulus Source: MGI
    11. cellular response to cytokine stimulus Source: MGI
    12. cellular response to interferon-alpha Source: Ensembl
    13. cellular response to interleukin-4 Source: UniProtKB
    14. cellular response to tumor necrosis factor Source: Ensembl
    15. developmental growth Source: MGI
    16. ear development Source: UniProtKB
    17. embryonic hemopoiesis Source: MGI
    18. embryonic organ development Source: MGI
    19. erythrocyte differentiation Source: MGI
    20. homeostasis of number of cells Source: MGI
    21. humoral immune response Source: MGI
    22. innate immune response Source: UniProtKB-KW
    23. inner ear morphogenesis Source: MGI
    24. interferon-gamma secretion Source: MGI
    25. interleukin-4 secretion Source: MGI
    26. in utero embryonic development Source: MGI
    27. lens development in camera-type eye Source: MGI
    28. lymphocyte migration Source: UniProtKB
    29. male gonad development Source: UniProtKB
    30. mast cell differentiation Source: MGI
    31. mesenchymal to epithelial transition Source: UniProtKB
    32. mesonephros development Source: MGI
    33. negative regulation of cell cycle Source: UniProtKB
    34. negative regulation of cell motility Source: UniProtKB
    35. negative regulation of cell proliferation Source: MGI
    36. negative regulation of cell proliferation involved in mesonephros development Source: UniProtKB
    37. negative regulation of endothelial cell apoptotic process Source: UniProtKB
    38. negative regulation of fat cell differentiation Source: UniProtKB
    39. negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation Source: UniProtKB
    40. negative regulation of gene expression Source: MGI
    41. negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation Source: UniProtKB
    42. negative regulation of inflammatory response Source: UniProtKB
    43. negative regulation of interferon-gamma production Source: MGI
    44. negative regulation of interleukin-2 production Source: MGI
    45. negative regulation of mammary gland epithelial cell proliferation Source: Ensembl
    46. negative regulation of transcription, DNA-templated Source: UniProtKB
    47. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    48. nephric duct formation Source: UniProtKB
    49. nephric duct morphogenesis Source: UniProtKB
    50. nervous system development Source: MGI
    51. neuron differentiation Source: MGI
    52. neuron migration Source: MGI
    53. norepinephrine biosynthetic process Source: MGI
    54. otic vesicle development Source: MGI
    55. parathyroid gland development Source: MGI
    56. parathyroid hormone secretion Source: MGI
    57. pharyngeal system development Source: UniProtKB
    58. phosphatidylinositol 3-kinase signaling Source: UniProtKB
    59. positive regulation of cell differentiation Source: MGI
    60. positive regulation of cytokine production Source: MGI
    61. positive regulation of endothelial cell migration Source: UniProtKB
    62. positive regulation of interleukin-13 production Source: MGI
    63. positive regulation of interleukin-13 secretion Source: UniProtKB
    64. positive regulation of interleukin-4 production Source: MGI
    65. positive regulation of interleukin-5 production Source: MGI
    66. positive regulation of interleukin-5 secretion Source: UniProtKB
    67. positive regulation of protein kinase B signaling Source: UniProtKB
    68. positive regulation of signal transduction Source: UniProtKB
    69. positive regulation of T cell differentiation Source: UniProtKB
    70. positive regulation of thyroid hormone generation Source: UniProtKB
    71. positive regulation of transcription, DNA-templated Source: UniProtKB
    72. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    73. positive regulation of transcription regulatory region DNA binding Source: UniProtKB
    74. positive regulation of ureteric bud formation Source: UniProtKB
    75. post-embryonic development Source: MGI
    76. pro-T cell differentiation Source: MGI
    77. regulation of CD4-positive, alpha-beta T cell differentiation Source: MGI
    78. regulation of cellular response to X-ray Source: UniProtKB
    79. regulation of cytokine biosynthetic process Source: MGI
    80. regulation of establishment of cell polarity Source: MGI
    81. regulation of histone H3-K27 methylation Source: MGI
    82. regulation of histone H3-K4 methylation Source: MGI
    83. regulation of nephron tubule epithelial cell differentiation Source: UniProtKB
    84. regulation of neuron apoptotic process Source: MGI
    85. regulation of neuron projection development Source: MGI
    86. regulation of transcription from RNA polymerase II promoter Source: MGI
    87. renal system development Source: MGI
    88. response to drug Source: Ensembl
    89. response to estrogen Source: Ensembl
    90. response to ethanol Source: Ensembl
    91. response to gamma radiation Source: Ensembl
    92. response to virus Source: Ensembl
    93. signal transduction Source: UniProtKB
    94. sympathetic nervous system development Source: MGI
    95. T cell differentiation Source: MGI
    96. T cell differentiation in thymus Source: MGI
    97. T cell receptor signaling pathway Source: UniProtKB
    98. T-helper 2 cell differentiation Source: MGI
    99. thymic T cell selection Source: MGI
    100. thymus development Source: MGI
    101. TOR signaling Source: UniProtKB
    102. type IV hypersensitivity Source: Ensembl
    103. ureteric bud formation Source: UniProtKB
    104. ureter maturation Source: MGI
    105. uterus development Source: UniProtKB
    106. ventricular septum development Source: UniProtKB

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Immunity, Innate immunity, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trans-acting T-cell-specific transcription factor GATA-3
    Alternative name(s):
    GATA-binding factor 3
    Gene namesi
    Name:Gata3
    Synonyms:Gata-3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:95663. Gata3.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear chromatin Source: Ensembl
    2. nucleolus Source: Ensembl
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi344 – 3441Y → A: Dramatically decreased Th2 cell differentiation. 1 Publication
    Mutagenesisi346 – 3461K → A: Moderately decreased Th2 cell differentiation. 1 Publication
    Mutagenesisi348 – 3481H → A: Dramatically decreased Th2 cell differentiation. 1 Publication
    Mutagenesisi352 – 3521R → A: Fails to induce Th2 cytokine production. 1 Publication
    Mutagenesisi353 – 3531P → A or K: Fails to induce Th2 cytokine production. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 443443Trans-acting T-cell-specific transcription factor GATA-3PRO_0000083409Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei114 – 1141PhosphoserineBy similarity
    Modified residuei161 – 1611PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP23772.

    PTM databases

    PhosphoSiteiP23772.

    Expressioni

    Tissue specificityi

    T-cell specific.

    Gene expression databases

    ArrayExpressiP23772.
    BgeeiP23772.
    CleanExiMM_GATA3.
    GenevestigatoriP23772.

    Interactioni

    Protein-protein interaction databases

    BioGridi199840. 5 interactions.
    DIPiDIP-29712N.
    IntActiP23772. 1 interaction.

    Structurei

    Secondary structure

    1
    443
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni318 – 3203
    Beta strandi326 – 3305
    Beta strandi336 – 3383
    Helixi339 – 34810
    Helixi354 – 3563

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3DFVX-ray3.10C/D308-370[»]
    3DFXX-ray2.70A/B308-370[»]
    ProteinModelPortaliP23772.
    SMRiP23772. Positions 261-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23772.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni288 – 31629Flexible linkerBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi344 – 35310YxKxHxxxRP

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi136 – 1394Poly-Ser
    Compositional biasi322 – 3265Poly-Thr

    Domaini

    Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation By similarity.By similarity
    The YxKxHxxxRP motif is critical for DNA-binding and function.1 Publication

    Sequence similaritiesi

    Contains 2 GATA-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri263 – 28725GATA-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri317 – 34125GATA-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5641.
    HOGENOMiHOG000047701.
    HOVERGENiHBG051705.
    InParanoidiP23772.
    KOiK17895.
    OMAiHGSQVCR.
    OrthoDBiEOG7CCBRF.
    PhylomeDBiP23772.
    TreeFamiTF315391.

    Family and domain databases

    Gene3Di3.30.50.10. 2 hits.
    InterProiIPR029521. GATA-3.
    IPR016374. TF_GATA-1/2/3.
    IPR000679. Znf_GATA.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PANTHERiPTHR10071:SF106. PTHR10071:SF106. 1 hit.
    PfamiPF00320. GATA. 2 hits.
    [Graphical view]
    PIRSFiPIRSF003027. TF_GATA-1/2/3. 1 hit.
    PRINTSiPR00619. GATAZNFINGER.
    SMARTiSM00401. ZnF_GATA. 2 hits.
    [Graphical view]
    PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
    PS50114. GATA_ZN_FINGER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23772-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LGHSYMEAQY PLTEEVDVLF    50
    NIDGQGNHVP SYYGNSVRAT VQRYPPTHHG SQVCRPPLLH GSLPWLDGGK 100
    ALSSHHTASP WNLSPFSKTS IHHGSPGPLS VYPPASSSSL AAGHSSPHLF 150
    TFPPTPPKDV SPDPSLSTPG SAGSARQDEK ECLKYQVQLP DSMKLETSHS 200
    RGSMTTLGGA SSSAHHPITT YPPYVPEYSS GLFPPSSLLG GSPTGFGCKS 250
    RPKARSSTEG RECVNCGATS TPLWRRDGTG HYLCNACGLY HKMNGQNRPL 300
    IKPKRRLSAA RRAGTSCANC QTTTTTLWRR NANGDPVCNA CGLYYKLHNI 350
    NRPLTMKKEG IQTRNRKMSS KSKKCKKVHD ALEDFPKSSS FNPAALSRHM 400
    SSLSHISPFS HSSHMLTTPT PMHPPSGLSF GPHHPSSMVT AMG 443
    Length:443
    Mass (Da):47,968
    Last modified:November 1, 1991 - v1
    Checksum:i980E58FB9872E560
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55123 mRNA. Translation: CAA38917.1.
    BC062915 mRNA. Translation: AAH62915.1.
    CCDSiCCDS15674.1.
    PIRiB39794.
    RefSeqiNP_032117.1. NM_008091.3.
    XP_006497416.1. XM_006497353.1.
    XP_006497417.1. XM_006497354.1.
    UniGeneiMm.313866.

    Genome annotation databases

    EnsembliENSMUST00000102976; ENSMUSP00000100041; ENSMUSG00000015619.
    GeneIDi14462.
    KEGGimmu:14462.
    UCSCiuc008ihf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55123 mRNA. Translation: CAA38917.1 .
    BC062915 mRNA. Translation: AAH62915.1 .
    CCDSi CCDS15674.1.
    PIRi B39794.
    RefSeqi NP_032117.1. NM_008091.3.
    XP_006497416.1. XM_006497353.1.
    XP_006497417.1. XM_006497354.1.
    UniGenei Mm.313866.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3DFV X-ray 3.10 C/D 308-370 [» ]
    3DFX X-ray 2.70 A/B 308-370 [» ]
    ProteinModelPortali P23772.
    SMRi P23772. Positions 261-365.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199840. 5 interactions.
    DIPi DIP-29712N.
    IntActi P23772. 1 interaction.

    PTM databases

    PhosphoSitei P23772.

    Proteomic databases

    PRIDEi P23772.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102976 ; ENSMUSP00000100041 ; ENSMUSG00000015619 .
    GeneIDi 14462.
    KEGGi mmu:14462.
    UCSCi uc008ihf.1. mouse.

    Organism-specific databases

    CTDi 2625.
    MGIi MGI:95663. Gata3.

    Phylogenomic databases

    eggNOGi COG5641.
    HOGENOMi HOG000047701.
    HOVERGENi HBG051705.
    InParanoidi P23772.
    KOi K17895.
    OMAi HGSQVCR.
    OrthoDBi EOG7CCBRF.
    PhylomeDBi P23772.
    TreeFami TF315391.

    Enzyme and pathway databases

    Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.

    Miscellaneous databases

    EvolutionaryTracei P23772.
    NextBioi 286100.
    PROi P23772.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P23772.
    Bgeei P23772.
    CleanExi MM_GATA3.
    Genevestigatori P23772.

    Family and domain databases

    Gene3Di 3.30.50.10. 2 hits.
    InterProi IPR029521. GATA-3.
    IPR016374. TF_GATA-1/2/3.
    IPR000679. Znf_GATA.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    PANTHERi PTHR10071:SF106. PTHR10071:SF106. 1 hit.
    Pfami PF00320. GATA. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF003027. TF_GATA-1/2/3. 1 hit.
    PRINTSi PR00619. GATAZNFINGER.
    SMARTi SM00401. ZnF_GATA. 2 hits.
    [Graphical view ]
    PROSITEi PS00344. GATA_ZN_FINGER_1. 2 hits.
    PS50114. GATA_ZN_FINGER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer."
      Ko L.J., Yamamoto M., Leonard M.W., George K.M., Ting P., Engel J.D.
      Mol. Cell. Biol. 11:2778-2784(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Critical YxKxHxxxRP motif in the C-terminal region of GATA3 for its DNA binding and function."
      Shinnakasu R., Yamashita M., Shinoda K., Endo Y., Hosokawa H., Hasegawa A., Ikemizu S., Nakayama T.
      J. Immunol. 177:5801-5810(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, YXKXHXXXRP MOTIF, MUTAGENESIS OF TYR-344; LYS-346; HIS-348; ARG-352 AND PRO-353.
    4. "Crystal structures of multiple GATA zinc fingers bound to DNA reveal new insights into DNA recognition and self-association by GATA."
      Bates D.L., Chen Y., Kim G., Guo L., Chen L.
      J. Mol. Biol. 381:1292-1306(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 308-370 IN COMPLEX WITH DNA, DOMAIN.

    Entry informationi

    Entry nameiGATA3_MOUSE
    AccessioniPrimary (citable) accession number: P23772
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3