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Protein

Trans-acting T-cell-specific transcription factor GATA-3

Gene

Gata3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri263 – 28725GATA-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri317 – 34125GATA-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • anatomical structure formation involved in morphogenesis Source: MGI
  • aortic valve morphogenesis Source: UniProtKB
  • axon guidance Source: MGI
  • canonical Wnt signaling pathway involved in metanephric kidney development Source: UniProtKB
  • cardiac right ventricle morphogenesis Source: UniProtKB
  • cell activation Source: MGI
  • cell fate determination Source: MGI
  • cell maturation Source: MGI
  • cell morphogenesis Source: MGI
  • cellular response to BMP stimulus Source: MGI
  • cellular response to cytokine stimulus Source: MGI
  • cellular response to interferon-alpha Source: Ensembl
  • cellular response to interleukin-4 Source: UniProtKB
  • cellular response to tumor necrosis factor Source: Ensembl
  • developmental growth Source: MGI
  • ear development Source: UniProtKB
  • embryonic hemopoiesis Source: MGI
  • embryonic organ development Source: MGI
  • erythrocyte differentiation Source: MGI
  • homeostasis of number of cells Source: MGI
  • humoral immune response Source: MGI
  • innate immune response Source: UniProtKB-KW
  • inner ear morphogenesis Source: MGI
  • interferon-gamma secretion Source: MGI
  • interleukin-4 secretion Source: MGI
  • in utero embryonic development Source: MGI
  • kidney development Source: MGI
  • lens development in camera-type eye Source: MGI
  • lymphocyte migration Source: UniProtKB
  • male gonad development Source: UniProtKB
  • mast cell differentiation Source: MGI
  • mesenchymal to epithelial transition Source: UniProtKB
  • mesonephros development Source: MGI
  • negative regulation of cell cycle Source: UniProtKB
  • negative regulation of cell motility Source: UniProtKB
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of cell proliferation involved in mesonephros development Source: UniProtKB
  • negative regulation of endothelial cell apoptotic process Source: UniProtKB
  • negative regulation of fat cell differentiation Source: UniProtKB
  • negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation Source: UniProtKB
  • negative regulation of gene expression Source: MGI
  • negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation Source: UniProtKB
  • negative regulation of inflammatory response Source: UniProtKB
  • negative regulation of interferon-gamma production Source: MGI
  • negative regulation of interleukin-2 production Source: MGI
  • negative regulation of mammary gland epithelial cell proliferation Source: MGI
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • nephric duct formation Source: UniProtKB
  • nephric duct morphogenesis Source: UniProtKB
  • nervous system development Source: MGI
  • neuron differentiation Source: MGI
  • neuron migration Source: MGI
  • norepinephrine biosynthetic process Source: MGI
  • otic vesicle development Source: MGI
  • parathyroid gland development Source: MGI
  • parathyroid hormone secretion Source: MGI
  • pharyngeal system development Source: UniProtKB
  • phosphatidylinositol 3-kinase signaling Source: UniProtKB
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of cytokine production Source: MGI
  • positive regulation of endothelial cell migration Source: UniProtKB
  • positive regulation of interleukin-13 production Source: MGI
  • positive regulation of interleukin-13 secretion Source: UniProtKB
  • positive regulation of interleukin-4 production Source: MGI
  • positive regulation of interleukin-5 production Source: MGI
  • positive regulation of interleukin-5 secretion Source: UniProtKB
  • positive regulation of protein kinase B signaling Source: UniProtKB
  • positive regulation of signal transduction Source: UniProtKB
  • positive regulation of T cell differentiation Source: UniProtKB
  • positive regulation of thyroid hormone generation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of transcription regulatory region DNA binding Source: UniProtKB
  • positive regulation of ureteric bud formation Source: UniProtKB
  • post-embryonic development Source: MGI
  • pro-T cell differentiation Source: MGI
  • regulation of CD4-positive, alpha-beta T cell differentiation Source: MGI
  • regulation of cellular response to X-ray Source: UniProtKB
  • regulation of cytokine biosynthetic process Source: MGI
  • regulation of establishment of cell polarity Source: MGI
  • regulation of histone H3-K27 methylation Source: MGI
  • regulation of histone H3-K4 methylation Source: MGI
  • regulation of nephron tubule epithelial cell differentiation Source: UniProtKB
  • regulation of neuron apoptotic process Source: MGI
  • regulation of neuron projection development Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • renal system development Source: MGI
  • response to drug Source: Ensembl
  • response to estrogen Source: Ensembl
  • response to ethanol Source: Ensembl
  • response to gamma radiation Source: Ensembl
  • response to virus Source: Ensembl
  • signal transduction Source: UniProtKB
  • sympathetic nervous system development Source: MGI
  • T cell differentiation Source: MGI
  • T cell differentiation in thymus Source: MGI
  • T cell receptor signaling pathway Source: UniProtKB
  • T-helper 2 cell differentiation Source: MGI
  • thymic T cell selection Source: MGI
  • thymus development Source: MGI
  • TOR signaling Source: UniProtKB
  • type IV hypersensitivity Source: Ensembl
  • ureteric bud formation Source: UniProtKB
  • ureter maturation Source: MGI
  • uterus development Source: UniProtKB
  • ventricular septum development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Immunity, Innate immunity, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_280010. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-acting T-cell-specific transcription factor GATA-3
Alternative name(s):
GATA-binding factor 3
Gene namesi
Name:Gata3
Synonyms:Gata-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95663. Gata3.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromatin Source: MGI
  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi344 – 3441Y → A: Dramatically decreased Th2 cell differentiation. 1 Publication
Mutagenesisi346 – 3461K → A: Moderately decreased Th2 cell differentiation. 1 Publication
Mutagenesisi348 – 3481H → A: Dramatically decreased Th2 cell differentiation. 1 Publication
Mutagenesisi352 – 3521R → A: Fails to induce Th2 cytokine production. 1 Publication
Mutagenesisi353 – 3531P → A or K: Fails to induce Th2 cytokine production. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Trans-acting T-cell-specific transcription factor GATA-3PRO_0000083409Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei114 – 1141PhosphoserineBy similarity
Modified residuei161 – 1611PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP23772.

PTM databases

PhosphoSiteiP23772.

Expressioni

Tissue specificityi

T-cell specific.

Gene expression databases

BgeeiP23772.
CleanExiMM_GATA3.
ExpressionAtlasiP23772. baseline and differential.
GenevisibleiP23772. MM.

Interactioni

Protein-protein interaction databases

BioGridi199840. 5 interactions.
DIPiDIP-29712N.
IntActiP23772. 1 interaction.
STRINGi10090.ENSMUSP00000100041.

Structurei

Secondary structure

1
443
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni318 – 3203Combined sources
Beta strandi326 – 3305Combined sources
Beta strandi336 – 3383Combined sources
Helixi339 – 34810Combined sources
Helixi354 – 3563Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DFVX-ray3.10C/D308-370[»]
3DFXX-ray2.70A/B308-370[»]
ProteinModelPortaliP23772.
SMRiP23772. Positions 261-365.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23772.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni288 – 31629Flexible linkerBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi344 – 35310YxKxHxxxRP

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi136 – 1394Poly-Ser
Compositional biasi322 – 3265Poly-Thr

Domaini

Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation (By similarity).By similarity
The YxKxHxxxRP motif is critical for DNA-binding and function.1 Publication

Sequence similaritiesi

Contains 2 GATA-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri263 – 28725GATA-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri317 – 34125GATA-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5641.
HOGENOMiHOG000047701.
HOVERGENiHBG051705.
InParanoidiP23772.
KOiK17895.
OMAiQRYPPTH.
OrthoDBiEOG7CCBRF.
PhylomeDBiP23772.
TreeFamiTF315391.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR029521. GATA-3.
IPR016374. TF_GATA-2/3.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF106. PTHR10071:SF106. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PIRSFiPIRSF003027. TF_GATA-1/2/3. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LGHSYMEAQY PLTEEVDVLF
60 70 80 90 100
NIDGQGNHVP SYYGNSVRAT VQRYPPTHHG SQVCRPPLLH GSLPWLDGGK
110 120 130 140 150
ALSSHHTASP WNLSPFSKTS IHHGSPGPLS VYPPASSSSL AAGHSSPHLF
160 170 180 190 200
TFPPTPPKDV SPDPSLSTPG SAGSARQDEK ECLKYQVQLP DSMKLETSHS
210 220 230 240 250
RGSMTTLGGA SSSAHHPITT YPPYVPEYSS GLFPPSSLLG GSPTGFGCKS
260 270 280 290 300
RPKARSSTEG RECVNCGATS TPLWRRDGTG HYLCNACGLY HKMNGQNRPL
310 320 330 340 350
IKPKRRLSAA RRAGTSCANC QTTTTTLWRR NANGDPVCNA CGLYYKLHNI
360 370 380 390 400
NRPLTMKKEG IQTRNRKMSS KSKKCKKVHD ALEDFPKSSS FNPAALSRHM
410 420 430 440
SSLSHISPFS HSSHMLTTPT PMHPPSGLSF GPHHPSSMVT AMG
Length:443
Mass (Da):47,968
Last modified:November 1, 1991 - v1
Checksum:i980E58FB9872E560
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55123 mRNA. Translation: CAA38917.1.
BC062915 mRNA. Translation: AAH62915.1.
CCDSiCCDS15674.1.
PIRiB39794.
RefSeqiNP_032117.1. NM_008091.3.
XP_006497416.1. XM_006497353.2.
XP_006497417.1. XM_006497354.1.
UniGeneiMm.313866.

Genome annotation databases

EnsembliENSMUST00000102976; ENSMUSP00000100041; ENSMUSG00000015619.
GeneIDi14462.
KEGGimmu:14462.
UCSCiuc008ihf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55123 mRNA. Translation: CAA38917.1.
BC062915 mRNA. Translation: AAH62915.1.
CCDSiCCDS15674.1.
PIRiB39794.
RefSeqiNP_032117.1. NM_008091.3.
XP_006497416.1. XM_006497353.2.
XP_006497417.1. XM_006497354.1.
UniGeneiMm.313866.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DFVX-ray3.10C/D308-370[»]
3DFXX-ray2.70A/B308-370[»]
ProteinModelPortaliP23772.
SMRiP23772. Positions 261-365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199840. 5 interactions.
DIPiDIP-29712N.
IntActiP23772. 1 interaction.
STRINGi10090.ENSMUSP00000100041.

PTM databases

PhosphoSiteiP23772.

Proteomic databases

PRIDEiP23772.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102976; ENSMUSP00000100041; ENSMUSG00000015619.
GeneIDi14462.
KEGGimmu:14462.
UCSCiuc008ihf.1. mouse.

Organism-specific databases

CTDi2625.
MGIiMGI:95663. Gata3.

Phylogenomic databases

eggNOGiCOG5641.
HOGENOMiHOG000047701.
HOVERGENiHBG051705.
InParanoidiP23772.
KOiK17895.
OMAiQRYPPTH.
OrthoDBiEOG7CCBRF.
PhylomeDBiP23772.
TreeFamiTF315391.

Enzyme and pathway databases

ReactomeiREACT_280010. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

EvolutionaryTraceiP23772.
NextBioi286100.
PROiP23772.
SOURCEiSearch...

Gene expression databases

BgeeiP23772.
CleanExiMM_GATA3.
ExpressionAtlasiP23772. baseline and differential.
GenevisibleiP23772. MM.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR029521. GATA-3.
IPR016374. TF_GATA-2/3.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF106. PTHR10071:SF106. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PIRSFiPIRSF003027. TF_GATA-1/2/3. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer."
    Ko L.J., Yamamoto M., Leonard M.W., George K.M., Ting P., Engel J.D.
    Mol. Cell. Biol. 11:2778-2784(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Critical YxKxHxxxRP motif in the C-terminal region of GATA3 for its DNA binding and function."
    Shinnakasu R., Yamashita M., Shinoda K., Endo Y., Hosokawa H., Hasegawa A., Ikemizu S., Nakayama T.
    J. Immunol. 177:5801-5810(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, YXKXHXXXRP MOTIF, MUTAGENESIS OF TYR-344; LYS-346; HIS-348; ARG-352 AND PRO-353.
  4. "Crystal structures of multiple GATA zinc fingers bound to DNA reveal new insights into DNA recognition and self-association by GATA."
    Bates D.L., Chen Y., Kim G., Guo L., Chen L.
    J. Mol. Biol. 381:1292-1306(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 308-370 IN COMPLEX WITH DNA, DOMAIN.

Entry informationi

Entry nameiGATA3_MOUSE
AccessioniPrimary (citable) accession number: P23772
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 24, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.