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P23772

- GATA3_MOUSE

UniProt

P23772 - GATA3_MOUSE

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Protein
Trans-acting T-cell-specific transcription factor GATA-3
Gene
Gata3, Gata-3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri263 – 28725GATA-type 1
Add
BLAST
Zinc fingeri317 – 34125GATA-type 2
Add
BLAST

GO - Molecular functioni

  1. E-box binding Source: UniProtKB
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: UniProtKB
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  4. RNA polymerase II core promoter sequence-specific DNA binding Source: MGI
  5. RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
  6. core promoter proximal region sequence-specific DNA binding Source: UniProtKB
  7. enhancer sequence-specific DNA binding Source: UniProtKB
  8. interleukin-2 receptor binding Source: MGI
  9. protein binding Source: UniProtKB
  10. protein dimerization activity Source: MGI
  11. sequence-specific DNA binding Source: MGI
  12. sequence-specific DNA binding transcription factor activity Source: UniProtKB
  13. transcription coactivator activity Source: UniProtKB
  14. transcription regulatory region DNA binding Source: UniProtKB
  15. zinc ion binding Source: InterPro

GO - Biological processi

  1. T cell differentiation Source: MGI
  2. T cell differentiation in thymus Source: MGI
  3. T cell receptor signaling pathway Source: UniProtKB
  4. T-helper 2 cell differentiation Source: MGI
  5. TOR signaling Source: UniProtKB
  6. anatomical structure formation involved in morphogenesis Source: MGI
  7. aortic valve morphogenesis Source: UniProtKB
  8. axon guidance Source: MGI
  9. canonical Wnt signaling pathway involved in metanephric kidney development Source: UniProtKB
  10. cardiac right ventricle morphogenesis Source: UniProtKB
  11. cell activation Source: MGI
  12. cell fate determination Source: MGI
  13. cell maturation Source: MGI
  14. cell morphogenesis Source: MGI
  15. cellular response to BMP stimulus Source: MGI
  16. cellular response to cytokine stimulus Source: MGI
  17. cellular response to interferon-alpha Source: Ensembl
  18. cellular response to interleukin-4 Source: UniProtKB
  19. cellular response to tumor necrosis factor Source: Ensembl
  20. developmental growth Source: MGI
  21. ear development Source: UniProtKB
  22. embryonic hemopoiesis Source: MGI
  23. embryonic organ development Source: MGI
  24. erythrocyte differentiation Source: MGI
  25. homeostasis of number of cells Source: MGI
  26. humoral immune response Source: MGI
  27. in utero embryonic development Source: MGI
  28. innate immune response Source: UniProtKB-KW
  29. inner ear morphogenesis Source: MGI
  30. interferon-gamma secretion Source: MGI
  31. interleukin-4 secretion Source: MGI
  32. lens development in camera-type eye Source: MGI
  33. lymphocyte migration Source: UniProtKB
  34. male gonad development Source: UniProtKB
  35. mast cell differentiation Source: MGI
  36. mesenchymal to epithelial transition Source: UniProtKB
  37. mesonephros development Source: MGI
  38. negative regulation of cell cycle Source: UniProtKB
  39. negative regulation of cell motility Source: UniProtKB
  40. negative regulation of cell proliferation Source: MGI
  41. negative regulation of cell proliferation involved in mesonephros development Source: UniProtKB
  42. negative regulation of endothelial cell apoptotic process Source: UniProtKB
  43. negative regulation of fat cell differentiation Source: UniProtKB
  44. negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation Source: UniProtKB
  45. negative regulation of gene expression Source: MGI
  46. negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation Source: UniProtKB
  47. negative regulation of inflammatory response Source: UniProtKB
  48. negative regulation of interferon-gamma production Source: MGI
  49. negative regulation of interleukin-2 production Source: MGI
  50. negative regulation of mammary gland epithelial cell proliferation Source: Ensembl
  51. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  52. negative regulation of transcription, DNA-templated Source: UniProtKB
  53. nephric duct formation Source: UniProtKB
  54. nephric duct morphogenesis Source: UniProtKB
  55. nervous system development Source: MGI
  56. neuron differentiation Source: MGI
  57. neuron migration Source: MGI
  58. norepinephrine biosynthetic process Source: MGI
  59. otic vesicle development Source: MGI
  60. parathyroid gland development Source: MGI
  61. parathyroid hormone secretion Source: MGI
  62. pharyngeal system development Source: UniProtKB
  63. phosphatidylinositol 3-kinase signaling Source: UniProtKB
  64. positive regulation of T cell differentiation Source: UniProtKB
  65. positive regulation of cell differentiation Source: MGI
  66. positive regulation of cytokine production Source: MGI
  67. positive regulation of endothelial cell migration Source: UniProtKB
  68. positive regulation of interleukin-13 production Source: MGI
  69. positive regulation of interleukin-13 secretion Source: UniProtKB
  70. positive regulation of interleukin-4 production Source: MGI
  71. positive regulation of interleukin-5 production Source: MGI
  72. positive regulation of interleukin-5 secretion Source: UniProtKB
  73. positive regulation of protein kinase B signaling Source: UniProtKB
  74. positive regulation of signal transduction Source: UniProtKB
  75. positive regulation of thyroid hormone generation Source: UniProtKB
  76. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  77. positive regulation of transcription regulatory region DNA binding Source: UniProtKB
  78. positive regulation of transcription, DNA-templated Source: UniProtKB
  79. positive regulation of ureteric bud formation Source: UniProtKB
  80. post-embryonic development Source: MGI
  81. pro-T cell differentiation Source: MGI
  82. regulation of CD4-positive, alpha-beta T cell differentiation Source: MGI
  83. regulation of cellular response to X-ray Source: UniProtKB
  84. regulation of cytokine biosynthetic process Source: MGI
  85. regulation of establishment of cell polarity Source: MGI
  86. regulation of histone H3-K27 methylation Source: MGI
  87. regulation of histone H3-K4 methylation Source: MGI
  88. regulation of nephron tubule epithelial cell differentiation Source: UniProtKB
  89. regulation of neuron apoptotic process Source: MGI
  90. regulation of neuron projection development Source: MGI
  91. regulation of transcription from RNA polymerase II promoter Source: MGI
  92. renal system development Source: MGI
  93. response to drug Source: Ensembl
  94. response to estrogen Source: Ensembl
  95. response to ethanol Source: Ensembl
  96. response to gamma radiation Source: Ensembl
  97. response to virus Source: Ensembl
  98. signal transduction Source: UniProtKB
  99. sympathetic nervous system development Source: MGI
  100. thymic T cell selection Source: MGI
  101. thymus development Source: MGI
  102. type IV hypersensitivity Source: Ensembl
  103. ureter maturation Source: MGI
  104. ureteric bud formation Source: UniProtKB
  105. uterus development Source: UniProtKB
  106. ventricular septum development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Immunity, Innate immunity, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-acting T-cell-specific transcription factor GATA-3
Alternative name(s):
GATA-binding factor 3
Gene namesi
Name:Gata3
Synonyms:Gata-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:95663. Gata3.

Subcellular locationi

GO - Cellular componenti

  1. nuclear chromatin Source: Ensembl
  2. nucleolus Source: Ensembl
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi344 – 3441Y → A: Dramatically decreased Th2 cell differentiation. 1 Publication
Mutagenesisi346 – 3461K → A: Moderately decreased Th2 cell differentiation. 1 Publication
Mutagenesisi348 – 3481H → A: Dramatically decreased Th2 cell differentiation. 1 Publication
Mutagenesisi352 – 3521R → A: Fails to induce Th2 cytokine production. 1 Publication
Mutagenesisi353 – 3531P → A or K: Fails to induce Th2 cytokine production. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Trans-acting T-cell-specific transcription factor GATA-3
PRO_0000083409Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei114 – 1141Phosphoserine By similarity
Modified residuei161 – 1611Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP23772.

PTM databases

PhosphoSiteiP23772.

Expressioni

Tissue specificityi

T-cell specific.

Gene expression databases

ArrayExpressiP23772.
BgeeiP23772.
CleanExiMM_GATA3.
GenevestigatoriP23772.

Interactioni

Protein-protein interaction databases

BioGridi199840. 5 interactions.
DIPiDIP-29712N.
IntActiP23772. 1 interaction.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni318 – 3203
Beta strandi326 – 3305
Beta strandi336 – 3383
Helixi339 – 34810
Helixi354 – 3563

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DFVX-ray3.10C/D308-370[»]
3DFXX-ray2.70A/B308-370[»]
ProteinModelPortaliP23772.
SMRiP23772. Positions 261-365.

Miscellaneous databases

EvolutionaryTraceiP23772.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni288 – 31629Flexible linker By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi344 – 35310YxKxHxxxRP

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi136 – 1394Poly-Ser
Compositional biasi322 – 3265Poly-Thr

Domaini

Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation By similarity.1 Publication
The YxKxHxxxRP motif is critical for DNA-binding and function.1 Publication

Sequence similaritiesi

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5641.
HOGENOMiHOG000047701.
HOVERGENiHBG051705.
InParanoidiP23772.
KOiK17895.
OMAiHGSQVCR.
OrthoDBiEOG7CCBRF.
PhylomeDBiP23772.
TreeFamiTF315391.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR029521. GATA-3.
IPR016374. TF_GATA-1/2/3.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF106. PTHR10071:SF106. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PIRSFiPIRSF003027. TF_GATA-1/2/3. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23772-1 [UniParc]FASTAAdd to Basket

« Hide

MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LGHSYMEAQY PLTEEVDVLF    50
NIDGQGNHVP SYYGNSVRAT VQRYPPTHHG SQVCRPPLLH GSLPWLDGGK 100
ALSSHHTASP WNLSPFSKTS IHHGSPGPLS VYPPASSSSL AAGHSSPHLF 150
TFPPTPPKDV SPDPSLSTPG SAGSARQDEK ECLKYQVQLP DSMKLETSHS 200
RGSMTTLGGA SSSAHHPITT YPPYVPEYSS GLFPPSSLLG GSPTGFGCKS 250
RPKARSSTEG RECVNCGATS TPLWRRDGTG HYLCNACGLY HKMNGQNRPL 300
IKPKRRLSAA RRAGTSCANC QTTTTTLWRR NANGDPVCNA CGLYYKLHNI 350
NRPLTMKKEG IQTRNRKMSS KSKKCKKVHD ALEDFPKSSS FNPAALSRHM 400
SSLSHISPFS HSSHMLTTPT PMHPPSGLSF GPHHPSSMVT AMG 443
Length:443
Mass (Da):47,968
Last modified:November 1, 1991 - v1
Checksum:i980E58FB9872E560
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55123 mRNA. Translation: CAA38917.1.
BC062915 mRNA. Translation: AAH62915.1.
CCDSiCCDS15674.1.
PIRiB39794.
RefSeqiNP_032117.1. NM_008091.3.
XP_006497416.1. XM_006497353.1.
XP_006497417.1. XM_006497354.1.
UniGeneiMm.313866.

Genome annotation databases

EnsembliENSMUST00000102976; ENSMUSP00000100041; ENSMUSG00000015619.
GeneIDi14462.
KEGGimmu:14462.
UCSCiuc008ihf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55123 mRNA. Translation: CAA38917.1 .
BC062915 mRNA. Translation: AAH62915.1 .
CCDSi CCDS15674.1.
PIRi B39794.
RefSeqi NP_032117.1. NM_008091.3.
XP_006497416.1. XM_006497353.1.
XP_006497417.1. XM_006497354.1.
UniGenei Mm.313866.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3DFV X-ray 3.10 C/D 308-370 [» ]
3DFX X-ray 2.70 A/B 308-370 [» ]
ProteinModelPortali P23772.
SMRi P23772. Positions 261-365.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199840. 5 interactions.
DIPi DIP-29712N.
IntActi P23772. 1 interaction.

PTM databases

PhosphoSitei P23772.

Proteomic databases

PRIDEi P23772.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000102976 ; ENSMUSP00000100041 ; ENSMUSG00000015619 .
GeneIDi 14462.
KEGGi mmu:14462.
UCSCi uc008ihf.1. mouse.

Organism-specific databases

CTDi 2625.
MGIi MGI:95663. Gata3.

Phylogenomic databases

eggNOGi COG5641.
HOGENOMi HOG000047701.
HOVERGENi HBG051705.
InParanoidi P23772.
KOi K17895.
OMAi HGSQVCR.
OrthoDBi EOG7CCBRF.
PhylomeDBi P23772.
TreeFami TF315391.

Enzyme and pathway databases

Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

EvolutionaryTracei P23772.
NextBioi 286100.
PROi P23772.
SOURCEi Search...

Gene expression databases

ArrayExpressi P23772.
Bgeei P23772.
CleanExi MM_GATA3.
Genevestigatori P23772.

Family and domain databases

Gene3Di 3.30.50.10. 2 hits.
InterProi IPR029521. GATA-3.
IPR016374. TF_GATA-1/2/3.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view ]
PANTHERi PTHR10071:SF106. PTHR10071:SF106. 1 hit.
Pfami PF00320. GATA. 2 hits.
[Graphical view ]
PIRSFi PIRSF003027. TF_GATA-1/2/3. 1 hit.
PRINTSi PR00619. GATAZNFINGER.
SMARTi SM00401. ZnF_GATA. 2 hits.
[Graphical view ]
PROSITEi PS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer."
    Ko L.J., Yamamoto M., Leonard M.W., George K.M., Ting P., Engel J.D.
    Mol. Cell. Biol. 11:2778-2784(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Critical YxKxHxxxRP motif in the C-terminal region of GATA3 for its DNA binding and function."
    Shinnakasu R., Yamashita M., Shinoda K., Endo Y., Hosokawa H., Hasegawa A., Ikemizu S., Nakayama T.
    J. Immunol. 177:5801-5810(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, YXKXHXXXRP MOTIF, MUTAGENESIS OF TYR-344; LYS-346; HIS-348; ARG-352 AND PRO-353.
  4. "Crystal structures of multiple GATA zinc fingers bound to DNA reveal new insights into DNA recognition and self-association by GATA."
    Bates D.L., Chen Y., Kim G., Guo L., Chen L.
    J. Mol. Biol. 381:1292-1306(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 308-370 IN COMPLEX WITH DNA, DOMAIN.

Entry informationi

Entry nameiGATA3_MOUSE
AccessioniPrimary (citable) accession number: P23772
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: September 3, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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