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Protein

Trans-acting T-cell-specific transcription factor GATA-3

Gene

Gata3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri263 – 28725GATA-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri317 – 34125GATA-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. core promoter proximal region sequence-specific DNA binding Source: UniProtKB
  2. core promoter sequence-specific DNA binding Source: MGI
  3. E-box binding Source: UniProtKB
  4. enhancer sequence-specific DNA binding Source: UniProtKB
  5. HMG box domain binding Source: MGI
  6. interleukin-2 receptor binding Source: MGI
  7. protein dimerization activity Source: MGI
  8. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: UniProtKB
  9. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  10. RNA polymerase II core promoter sequence-specific DNA binding Source: MGI
  11. RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
  12. sequence-specific DNA binding Source: MGI
  13. sequence-specific DNA binding transcription factor activity Source: UniProtKB
  14. transcription coactivator activity Source: UniProtKB
  15. transcription factor binding Source: MGI
  16. transcription regulatory region DNA binding Source: UniProtKB
  17. transcription regulatory region sequence-specific DNA binding Source: MGI
  18. zinc ion binding Source: InterPro

GO - Biological processi

  1. anatomical structure formation involved in morphogenesis Source: MGI
  2. aortic valve morphogenesis Source: UniProtKB
  3. axon guidance Source: MGI
  4. canonical Wnt signaling pathway involved in metanephric kidney development Source: UniProtKB
  5. cardiac right ventricle morphogenesis Source: UniProtKB
  6. cell activation Source: MGI
  7. cell fate determination Source: MGI
  8. cell maturation Source: MGI
  9. cell morphogenesis Source: MGI
  10. cellular response to BMP stimulus Source: MGI
  11. cellular response to cytokine stimulus Source: MGI
  12. cellular response to interferon-alpha Source: Ensembl
  13. cellular response to interleukin-4 Source: UniProtKB
  14. cellular response to tumor necrosis factor Source: Ensembl
  15. developmental growth Source: MGI
  16. ear development Source: UniProtKB
  17. embryonic hemopoiesis Source: MGI
  18. embryonic organ development Source: MGI
  19. erythrocyte differentiation Source: MGI
  20. homeostasis of number of cells Source: MGI
  21. humoral immune response Source: MGI
  22. innate immune response Source: UniProtKB-KW
  23. inner ear morphogenesis Source: MGI
  24. interferon-gamma secretion Source: MGI
  25. interleukin-4 secretion Source: MGI
  26. in utero embryonic development Source: MGI
  27. kidney development Source: MGI
  28. lens development in camera-type eye Source: MGI
  29. lymphocyte migration Source: UniProtKB
  30. male gonad development Source: UniProtKB
  31. mast cell differentiation Source: MGI
  32. mesenchymal to epithelial transition Source: UniProtKB
  33. mesonephros development Source: MGI
  34. negative regulation of cell cycle Source: UniProtKB
  35. negative regulation of cell motility Source: UniProtKB
  36. negative regulation of cell proliferation Source: MGI
  37. negative regulation of cell proliferation involved in mesonephros development Source: UniProtKB
  38. negative regulation of endothelial cell apoptotic process Source: UniProtKB
  39. negative regulation of fat cell differentiation Source: UniProtKB
  40. negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation Source: UniProtKB
  41. negative regulation of gene expression Source: MGI
  42. negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation Source: UniProtKB
  43. negative regulation of inflammatory response Source: UniProtKB
  44. negative regulation of interferon-gamma production Source: MGI
  45. negative regulation of interleukin-2 production Source: MGI
  46. negative regulation of mammary gland epithelial cell proliferation Source: MGI
  47. negative regulation of transcription, DNA-templated Source: UniProtKB
  48. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  49. nephric duct formation Source: UniProtKB
  50. nephric duct morphogenesis Source: UniProtKB
  51. nervous system development Source: MGI
  52. neuron differentiation Source: MGI
  53. neuron migration Source: MGI
  54. norepinephrine biosynthetic process Source: MGI
  55. otic vesicle development Source: MGI
  56. parathyroid gland development Source: MGI
  57. parathyroid hormone secretion Source: MGI
  58. pharyngeal system development Source: UniProtKB
  59. phosphatidylinositol 3-kinase signaling Source: UniProtKB
  60. positive regulation of cell differentiation Source: MGI
  61. positive regulation of cytokine production Source: MGI
  62. positive regulation of endothelial cell migration Source: UniProtKB
  63. positive regulation of interleukin-13 production Source: MGI
  64. positive regulation of interleukin-13 secretion Source: UniProtKB
  65. positive regulation of interleukin-4 production Source: MGI
  66. positive regulation of interleukin-5 production Source: MGI
  67. positive regulation of interleukin-5 secretion Source: UniProtKB
  68. positive regulation of protein kinase B signaling Source: UniProtKB
  69. positive regulation of signal transduction Source: UniProtKB
  70. positive regulation of T cell differentiation Source: UniProtKB
  71. positive regulation of thyroid hormone generation Source: UniProtKB
  72. positive regulation of transcription, DNA-templated Source: UniProtKB
  73. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  74. positive regulation of transcription regulatory region DNA binding Source: UniProtKB
  75. positive regulation of ureteric bud formation Source: UniProtKB
  76. post-embryonic development Source: MGI
  77. pro-T cell differentiation Source: MGI
  78. regulation of CD4-positive, alpha-beta T cell differentiation Source: MGI
  79. regulation of cellular response to X-ray Source: UniProtKB
  80. regulation of cytokine biosynthetic process Source: MGI
  81. regulation of establishment of cell polarity Source: MGI
  82. regulation of histone H3-K27 methylation Source: MGI
  83. regulation of histone H3-K4 methylation Source: MGI
  84. regulation of nephron tubule epithelial cell differentiation Source: UniProtKB
  85. regulation of neuron apoptotic process Source: MGI
  86. regulation of neuron projection development Source: MGI
  87. regulation of transcription from RNA polymerase II promoter Source: MGI
  88. renal system development Source: MGI
  89. response to drug Source: Ensembl
  90. response to estrogen Source: Ensembl
  91. response to ethanol Source: Ensembl
  92. response to gamma radiation Source: Ensembl
  93. response to virus Source: Ensembl
  94. signal transduction Source: UniProtKB
  95. sympathetic nervous system development Source: MGI
  96. T cell differentiation Source: MGI
  97. T cell differentiation in thymus Source: MGI
  98. T cell receptor signaling pathway Source: UniProtKB
  99. T-helper 2 cell differentiation Source: MGI
  100. thymic T cell selection Source: MGI
  101. thymus development Source: MGI
  102. TOR signaling Source: UniProtKB
  103. type IV hypersensitivity Source: Ensembl
  104. ureteric bud formation Source: UniProtKB
  105. ureter maturation Source: MGI
  106. uterus development Source: UniProtKB
  107. ventricular septum development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Immunity, Innate immunity, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_280010. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-acting T-cell-specific transcription factor GATA-3
Alternative name(s):
GATA-binding factor 3
Gene namesi
Name:Gata3
Synonyms:Gata-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95663. Gata3.

Subcellular locationi

GO - Cellular componenti

  1. nuclear chromatin Source: MGI
  2. nucleolus Source: MGI
  3. nucleoplasm Source: MGI
  4. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi344 – 3441Y → A: Dramatically decreased Th2 cell differentiation. 1 Publication
Mutagenesisi346 – 3461K → A: Moderately decreased Th2 cell differentiation. 1 Publication
Mutagenesisi348 – 3481H → A: Dramatically decreased Th2 cell differentiation. 1 Publication
Mutagenesisi352 – 3521R → A: Fails to induce Th2 cytokine production. 1 Publication
Mutagenesisi353 – 3531P → A or K: Fails to induce Th2 cytokine production. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Trans-acting T-cell-specific transcription factor GATA-3PRO_0000083409Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei114 – 1141PhosphoserineBy similarity
Modified residuei161 – 1611PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP23772.

PTM databases

PhosphoSiteiP23772.

Expressioni

Tissue specificityi

T-cell specific.

Gene expression databases

BgeeiP23772.
CleanExiMM_GATA3.
ExpressionAtlasiP23772. baseline and differential.
GenevestigatoriP23772.

Interactioni

Protein-protein interaction databases

BioGridi199840. 5 interactions.
DIPiDIP-29712N.
IntActiP23772. 1 interaction.

Structurei

Secondary structure

1
443
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni318 – 3203Combined sources
Beta strandi326 – 3305Combined sources
Beta strandi336 – 3383Combined sources
Helixi339 – 34810Combined sources
Helixi354 – 3563Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DFVX-ray3.10C/D308-370[»]
3DFXX-ray2.70A/B308-370[»]
ProteinModelPortaliP23772.
SMRiP23772. Positions 261-365.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23772.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni288 – 31629Flexible linkerBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi344 – 35310YxKxHxxxRP

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi136 – 1394Poly-Ser
Compositional biasi322 – 3265Poly-Thr

Domaini

Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation (By similarity).By similarity
The YxKxHxxxRP motif is critical for DNA-binding and function.1 Publication

Sequence similaritiesi

Contains 2 GATA-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri263 – 28725GATA-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri317 – 34125GATA-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5641.
HOGENOMiHOG000047701.
HOVERGENiHBG051705.
InParanoidiP23772.
KOiK17895.
OMAiQRYPPTH.
OrthoDBiEOG7CCBRF.
PhylomeDBiP23772.
TreeFamiTF315391.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR029521. GATA-3.
IPR016374. TF_GATA-2/3.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF106. PTHR10071:SF106. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PIRSFiPIRSF003027. TF_GATA-1/2/3. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LGHSYMEAQY PLTEEVDVLF
60 70 80 90 100
NIDGQGNHVP SYYGNSVRAT VQRYPPTHHG SQVCRPPLLH GSLPWLDGGK
110 120 130 140 150
ALSSHHTASP WNLSPFSKTS IHHGSPGPLS VYPPASSSSL AAGHSSPHLF
160 170 180 190 200
TFPPTPPKDV SPDPSLSTPG SAGSARQDEK ECLKYQVQLP DSMKLETSHS
210 220 230 240 250
RGSMTTLGGA SSSAHHPITT YPPYVPEYSS GLFPPSSLLG GSPTGFGCKS
260 270 280 290 300
RPKARSSTEG RECVNCGATS TPLWRRDGTG HYLCNACGLY HKMNGQNRPL
310 320 330 340 350
IKPKRRLSAA RRAGTSCANC QTTTTTLWRR NANGDPVCNA CGLYYKLHNI
360 370 380 390 400
NRPLTMKKEG IQTRNRKMSS KSKKCKKVHD ALEDFPKSSS FNPAALSRHM
410 420 430 440
SSLSHISPFS HSSHMLTTPT PMHPPSGLSF GPHHPSSMVT AMG
Length:443
Mass (Da):47,968
Last modified:October 31, 1991 - v1
Checksum:i980E58FB9872E560
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55123 mRNA. Translation: CAA38917.1.
BC062915 mRNA. Translation: AAH62915.1.
CCDSiCCDS15674.1.
PIRiB39794.
RefSeqiNP_032117.1. NM_008091.3.
XP_006497416.1. XM_006497353.2.
XP_006497417.1. XM_006497354.1.
UniGeneiMm.313866.

Genome annotation databases

EnsembliENSMUST00000102976; ENSMUSP00000100041; ENSMUSG00000015619.
GeneIDi14462.
KEGGimmu:14462.
UCSCiuc008ihf.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55123 mRNA. Translation: CAA38917.1.
BC062915 mRNA. Translation: AAH62915.1.
CCDSiCCDS15674.1.
PIRiB39794.
RefSeqiNP_032117.1. NM_008091.3.
XP_006497416.1. XM_006497353.2.
XP_006497417.1. XM_006497354.1.
UniGeneiMm.313866.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DFVX-ray3.10C/D308-370[»]
3DFXX-ray2.70A/B308-370[»]
ProteinModelPortaliP23772.
SMRiP23772. Positions 261-365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199840. 5 interactions.
DIPiDIP-29712N.
IntActiP23772. 1 interaction.

PTM databases

PhosphoSiteiP23772.

Proteomic databases

PRIDEiP23772.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102976; ENSMUSP00000100041; ENSMUSG00000015619.
GeneIDi14462.
KEGGimmu:14462.
UCSCiuc008ihf.1. mouse.

Organism-specific databases

CTDi2625.
MGIiMGI:95663. Gata3.

Phylogenomic databases

eggNOGiCOG5641.
HOGENOMiHOG000047701.
HOVERGENiHBG051705.
InParanoidiP23772.
KOiK17895.
OMAiQRYPPTH.
OrthoDBiEOG7CCBRF.
PhylomeDBiP23772.
TreeFamiTF315391.

Enzyme and pathway databases

ReactomeiREACT_280010. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

EvolutionaryTraceiP23772.
NextBioi286100.
PROiP23772.
SOURCEiSearch...

Gene expression databases

BgeeiP23772.
CleanExiMM_GATA3.
ExpressionAtlasiP23772. baseline and differential.
GenevestigatoriP23772.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR029521. GATA-3.
IPR016374. TF_GATA-2/3.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF106. PTHR10071:SF106. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PIRSFiPIRSF003027. TF_GATA-1/2/3. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer."
    Ko L.J., Yamamoto M., Leonard M.W., George K.M., Ting P., Engel J.D.
    Mol. Cell. Biol. 11:2778-2784(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Critical YxKxHxxxRP motif in the C-terminal region of GATA3 for its DNA binding and function."
    Shinnakasu R., Yamashita M., Shinoda K., Endo Y., Hosokawa H., Hasegawa A., Ikemizu S., Nakayama T.
    J. Immunol. 177:5801-5810(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, YXKXHXXXRP MOTIF, MUTAGENESIS OF TYR-344; LYS-346; HIS-348; ARG-352 AND PRO-353.
  4. "Crystal structures of multiple GATA zinc fingers bound to DNA reveal new insights into DNA recognition and self-association by GATA."
    Bates D.L., Chen Y., Kim G., Guo L., Chen L.
    J. Mol. Biol. 381:1292-1306(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 308-370 IN COMPLEX WITH DNA, DOMAIN.

Entry informationi

Entry nameiGATA3_MOUSE
AccessioniPrimary (citable) accession number: P23772
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1991
Last sequence update: October 31, 1991
Last modified: March 31, 2015
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.