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P23772 (GATA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trans-acting T-cell-specific transcription factor GATA-3
Alternative name(s):
GATA-binding factor 3
Gene names
Name:Gata3
Synonyms:Gata-3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length443 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.

Subcellular location

Nucleus.

Tissue specificity

T-cell specific.

Domain

Binds DNA via the 2 GATA-type zinc fingers. Ref.3

Sequence similarities

Contains 2 GATA-type zinc fingers.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT cell differentiation

Inferred from direct assay PubMed 7956841. Source: MGI

T cell differentiation in thymus

Inferred from mutant phenotype PubMed 14670303PubMed 19934022PubMed 8945476. Source: MGI

T cell receptor signaling pathway

Inferred from direct assay PubMed 20696860. Source: UniProtKB

T-helper 2 cell differentiation

Inferred from direct assay PubMed 20706986. Source: MGI

TOR signaling

Inferred from mutant phenotype PubMed 20696860. Source: UniProtKB

anatomical structure formation involved in morphogenesis

Inferred from mutant phenotype PubMed 16319112. Source: MGI

aortic valve morphogenesis

Inferred from mutant phenotype PubMed 18955134. Source: UniProtKB

axon guidance

Inferred from mutant phenotype PubMed 11135239. Source: MGI

canonical Wnt signaling pathway involved in metanephric kidney development

Inferred from mutant phenotype PubMed 19112489. Source: UniProtKB

cardiac right ventricle morphogenesis

Inferred from mutant phenotype PubMed 18955134. Source: UniProtKB

cell activation

Inferred from mutant phenotype PubMed 17082577. Source: MGI

cell fate determination

Inferred from direct assay PubMed 12923059. Source: MGI

cell maturation

Inferred from mutant phenotype PubMed 17082577. Source: MGI

cell morphogenesis

Inferred from mutant phenotype PubMed 8945476. Source: MGI

cellular response to BMP stimulus

Inferred from direct assay PubMed 20501701. Source: MGI

cellular response to cytokine stimulus

Inferred from direct assay PubMed 19666510. Source: MGI

cellular response to interferon-alpha

Inferred from electronic annotation. Source: Ensembl

cellular response to interleukin-4

Inferred from expression pattern PubMed 20696860. Source: UniProtKB

cellular response to tumor necrosis factor

Inferred from electronic annotation. Source: Ensembl

developmental growth

Inferred from mutant phenotype PubMed 7550312. Source: MGI

ear development

Inferred from sequence or structural similarity. Source: UniProtKB

embryonic hemopoiesis

Inferred from mutant phenotype PubMed 7550312. Source: MGI

embryonic organ development

Inferred from mutant phenotype PubMed 11135239. Source: MGI

erythrocyte differentiation

Inferred from direct assay PubMed 9576834. Source: MGI

homeostasis of number of cells

Inferred from mutant phenotype PubMed 19934022. Source: MGI

humoral immune response

Inferred from mutant phenotype PubMed 17658279. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 20702712. Source: MGI

inner ear morphogenesis

Inferred from mutant phenotype PubMed 11135239PubMed 21553382. Source: MGI

interferon-gamma secretion

Inferred from mutant phenotype PubMed 17658279. Source: MGI

interleukin-4 secretion

Inferred from mutant phenotype PubMed 17658279. Source: MGI

lens development in camera-type eye

Inferred from mutant phenotype PubMed 19623612. Source: MGI

lymphocyte migration

Inferred from sequence or structural similarity. Source: UniProtKB

male gonad development

Inferred from mutant phenotype PubMed 19112489. Source: UniProtKB

mast cell differentiation

Inferred from direct assay PubMed 17603486. Source: MGI

mesenchymal to epithelial transition

Inferred from sequence or structural similarity. Source: UniProtKB

mesonephros development

Inferred from mutant phenotype PubMed 16319112. Source: MGI

negative regulation of cell cycle

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell motility

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 16319112PubMed 19623612. Source: MGI

negative regulation of cell proliferation involved in mesonephros development

Inferred from mutant phenotype PubMed 16319112. Source: UniProtKB

negative regulation of endothelial cell apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of fat cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation

Inferred from mutant phenotype PubMed 19112489. Source: UniProtKB

negative regulation of gene expression

Inferred from mutant phenotype PubMed 17357106. Source: MGI

negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation

Inferred from mutant phenotype PubMed 19112489. Source: UniProtKB

negative regulation of inflammatory response

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of interferon-gamma production

Inferred from direct assay PubMed 18792410. Source: MGI

negative regulation of interleukin-2 production

Inferred from direct assay PubMed 18792410. Source: MGI

negative regulation of mammary gland epithelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18792410. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

nephric duct formation

Inferred from mutant phenotype PubMed 16319112. Source: UniProtKB

nephric duct morphogenesis

Inferred from mutant phenotype PubMed 19112489. Source: UniProtKB

nervous system development

Inferred from mutant phenotype PubMed 7550312. Source: MGI

neuron differentiation

Inferred from mutant phenotype PubMed 20818386. Source: MGI

neuron migration

Inferred from mutant phenotype PubMed 10556076. Source: MGI

norepinephrine biosynthetic process

Inferred from mutant phenotype PubMed 10835639. Source: MGI

otic vesicle development

Inferred from mutant phenotype PubMed 11135239. Source: MGI

parathyroid gland development

Inferred from mutant phenotype PubMed 20484821. Source: MGI

parathyroid hormone secretion

Inferred from mutant phenotype PubMed 20484821. Source: MGI

pharyngeal system development

Inferred from mutant phenotype PubMed 18955134. Source: UniProtKB

phosphatidylinositol 3-kinase signaling

Inferred from mutant phenotype PubMed 20696860. Source: UniProtKB

positive regulation of T cell differentiation

Inferred from direct assay PubMed 20696860. Source: UniProtKB

positive regulation of cell differentiation

Inferred from mutant phenotype PubMed 19623612. Source: MGI

positive regulation of cytokine production

Inferred from direct assay PubMed 18792410. Source: MGI

positive regulation of endothelial cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of interleukin-13 production

Inferred from mutant phenotype PubMed 10549629. Source: MGI

positive regulation of interleukin-13 secretion

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of interleukin-4 production

Inferred from direct assay PubMed 17658278. Source: MGI

positive regulation of interleukin-5 production

Inferred from mutant phenotype PubMed 10549629. Source: MGI

positive regulation of interleukin-5 secretion

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of protein kinase B signaling

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of signal transduction

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of thyroid hormone generation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 12127263PubMed 18776904Ref.1PubMed 21867929PubMed 7720565. Source: MGI

positive regulation of transcription regulatory region DNA binding

Inferred from mutant phenotype PubMed 20636338. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 20636338. Source: UniProtKB

positive regulation of ureteric bud formation

Inferred from mutant phenotype PubMed 19112489. Source: UniProtKB

post-embryonic development

Inferred from mutant phenotype PubMed 7550312. Source: MGI

pro-T cell differentiation

Inferred from mutant phenotype PubMed 19934022. Source: MGI

regulation of CD4-positive, alpha-beta T cell differentiation

Inferred from mutant phenotype PubMed 18776904. Source: MGI

regulation of cellular response to X-ray

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of cytokine biosynthetic process

Inferred from direct assay PubMed 11093124. Source: MGI

regulation of establishment of cell polarity

Inferred from direct assay PubMed 21613615. Source: MGI

regulation of histone H3-K27 methylation

Inferred from direct assay PubMed 21867929. Source: MGI

regulation of histone H3-K4 methylation

Inferred from direct assay PubMed 21867929. Source: MGI

regulation of nephron tubule epithelial cell differentiation

Inferred from mutant phenotype PubMed 19112489. Source: UniProtKB

regulation of neuron apoptotic process

Inferred from mutant phenotype PubMed 20702712. Source: MGI

regulation of neuron projection development

Inferred from mutant phenotype PubMed 23426694. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19796622PubMed 21536806. Source: MGI

renal system development

Inferred from genetic interaction PubMed 21731775. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

response to estrogen

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to gamma radiation

Inferred from electronic annotation. Source: Ensembl

response to virus

Inferred from electronic annotation. Source: Ensembl

signal transduction

Inferred from mutant phenotype PubMed 16319112. Source: UniProtKB

sympathetic nervous system development

Inferred from mutant phenotype PubMed 15329349. Source: MGI

thymic T cell selection

Inferred from mutant phenotype PubMed 14670303. Source: MGI

thymus development

Inferred from mutant phenotype PubMed 14643687PubMed 17082577. Source: MGI

type IV hypersensitivity

Inferred from electronic annotation. Source: Ensembl

ureter maturation

Inferred from mutant phenotype PubMed 21521737. Source: MGI

ureteric bud formation

Inferred from mutant phenotype PubMed 16319112. Source: UniProtKB

uterus development

Inferred from mutant phenotype PubMed 19112489. Source: UniProtKB

ventricular septum development

Inferred from mutant phenotype PubMed 18955134. Source: UniProtKB

   Cellular_componentnuclear chromatin

Inferred from electronic annotation. Source: Ensembl

nucleolus

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 20696860. Source: UniProtKB

   Molecular_functionE-box binding

Inferred from sequence or structural similarity. Source: UniProtKB

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription

Inferred from sequence or structural similarity. Source: UniProtKB

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 7720565. Source: MGI

RNA polymerase II core promoter sequence-specific DNA binding

Inferred from direct assay PubMed 10549629PubMed 20484821. Source: MGI

RNA polymerase II regulatory region sequence-specific DNA binding

Inferred from direct assay PubMed 18776904PubMed 20176728. Source: MGI

core promoter proximal region sequence-specific DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

enhancer sequence-specific DNA binding

Inferred from direct assay PubMed 20855530. Source: UniProtKB

interleukin-2 receptor binding

Inferred from physical interaction PubMed 21217760. Source: MGI

protein dimerization activity

Inferred from direct assay Ref.3. Source: MGI

sequence-specific DNA binding

Inferred from direct assay Ref.3PubMed 21867929. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 20636338. Source: UniProtKB

transcription coactivator activity

Inferred from sequence or structural similarity. Source: UniProtKB

transcription regulatory region DNA binding

Inferred from direct assay PubMed 20636338. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 443443Trans-acting T-cell-specific transcription factor GATA-3
PRO_0000083409

Regions

Zinc finger263 – 28725GATA-type 1
Zinc finger317 – 34125GATA-type 2
Compositional bias136 – 1394Poly-Ser
Compositional bias322 – 3265Poly-Thr

Amino acid modifications

Modified residue1141Phosphoserine By similarity
Modified residue1611Phosphoserine By similarity

Secondary structure

.......... 443
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P23772 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 980E58FB9872E560

FASTA44347,968
        10         20         30         40         50         60 
MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LGHSYMEAQY PLTEEVDVLF NIDGQGNHVP 

        70         80         90        100        110        120 
SYYGNSVRAT VQRYPPTHHG SQVCRPPLLH GSLPWLDGGK ALSSHHTASP WNLSPFSKTS 

       130        140        150        160        170        180 
IHHGSPGPLS VYPPASSSSL AAGHSSPHLF TFPPTPPKDV SPDPSLSTPG SAGSARQDEK 

       190        200        210        220        230        240 
ECLKYQVQLP DSMKLETSHS RGSMTTLGGA SSSAHHPITT YPPYVPEYSS GLFPPSSLLG 

       250        260        270        280        290        300 
GSPTGFGCKS RPKARSSTEG RECVNCGATS TPLWRRDGTG HYLCNACGLY HKMNGQNRPL 

       310        320        330        340        350        360 
IKPKRRLSAA RRAGTSCANC QTTTTTLWRR NANGDPVCNA CGLYYKLHNI NRPLTMKKEG 

       370        380        390        400        410        420 
IQTRNRKMSS KSKKCKKVHD ALEDFPKSSS FNPAALSRHM SSLSHISPFS HSSHMLTTPT 

       430        440 
PMHPPSGLSF GPHHPSSMVT AMG 

« Hide

References

« Hide 'large scale' references
[1]"Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer."
Ko L.J., Yamamoto M., Leonard M.W., George K.M., Ting P., Engel J.D.
Mol. Cell. Biol. 11:2778-2784(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"Crystal structures of multiple GATA zinc fingers bound to DNA reveal new insights into DNA recognition and self-association by GATA."
Bates D.L., Chen Y., Kim G., Guo L., Chen L.
J. Mol. Biol. 381:1292-1306(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 308-370 IN COMPLEX WITH DNA, DOMAIN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55123 mRNA. Translation: CAA38917.1.
BC062915 mRNA. Translation: AAH62915.1.
PIRB39794.
RefSeqNP_032117.1. NM_008091.3.
XP_006497416.1. XM_006497353.1.
XP_006497417.1. XM_006497354.1.
UniGeneMm.313866.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3DFVX-ray3.10C/D308-370[»]
3DFXX-ray2.70A/B308-370[»]
ProteinModelPortalP23772.
SMRP23772. Positions 261-365.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199840. 4 interactions.
DIPDIP-29712N.
IntActP23772. 1 interaction.

PTM databases

PhosphoSiteP23772.

Proteomic databases

PRIDEP23772.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102976; ENSMUSP00000100041; ENSMUSG00000015619.
GeneID14462.
KEGGmmu:14462.
UCSCuc008ihf.1. mouse.

Organism-specific databases

CTD2625.
MGIMGI:95663. Gata3.

Phylogenomic databases

eggNOGCOG5641.
HOGENOMHOG000047701.
HOVERGENHBG051705.
InParanoidP23772.
KOK17895.
OMAHHPSSMV.
OrthoDBEOG7CCBRF.
PhylomeDBP23772.
TreeFamTF315391.

Gene expression databases

ArrayExpressP23772.
BgeeP23772.
CleanExMM_GATA3.
GenevestigatorP23772.

Family and domain databases

Gene3D3.30.50.10. 2 hits.
InterProIPR016374. TF_GATA-1/2/3.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00320. GATA. 2 hits.
[Graphical view]
PIRSFPIRSF003027. TF_GATA-1/2/3. 1 hit.
PRINTSPR00619. GATAZNFINGER.
SMARTSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP23772.
NextBio286100.
PROP23772.
SOURCESearch...

Entry information

Entry nameGATA3_MOUSE
AccessionPrimary (citable) accession number: P23772
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 16, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot