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Protein

Trans-acting T-cell-specific transcription factor GATA-3

Gene

GATA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri263 – 287GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri317 – 341GATA-type 2PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Immunity, Innate immunity, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000107485-MONOMER.
ReactomeiR-HSA-5689880. Ub-specific processing proteases.
R-HSA-983231. Factors involved in megakaryocyte development and platelet production.
SignaLinkiP23771.
SIGNORiP23771.

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-acting T-cell-specific transcription factor GATA-3
Alternative name(s):
GATA-binding factor 3
Gene namesi
Name:GATA3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:4172. GATA3.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromatin Source: BHF-UCL
  • nucleolus Source: HPA
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • transcription factor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Hypoparathyroidism, sensorineural deafness, and renal disease (HDR)3 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disease characterized by steroid-resistant nephrosis with progressive renal failure, hypoparathyroidism, sensorineural deafness, and renal dysplasia.
See also OMIM:146255
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017818274W → R in HDR. 1 PublicationCorresponds to variant rs104894163dbSNPEnsembl.1
Natural variantiVAR_075427298R → Q in HDR; loss of enhancer activity on PTH gene promoter and on GATA responsive element. 1 Publication1

Keywords - Diseasei

Deafness, Disease mutation

Organism-specific databases

DisGeNETi2625.
MalaCardsiGATA3.
MIMi146255. phenotype.
OpenTargetsiENSG00000107485.
Orphaneti2237. Hypoparathyroidism - deafness - renal disease.
99860. Precursor B-cell acute lymphoblastic leukemia.
PharmGKBiPA28586.

Polymorphism and mutation databases

BioMutaiGATA3.
DMDMi120962.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834081 – 443Trans-acting T-cell-specific transcription factor GATA-3Add BLAST443

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei115PhosphoserineCombined sources1
Modified residuei162PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP23771.
MaxQBiP23771.
PaxDbiP23771.
PeptideAtlasiP23771.
PRIDEiP23771.

PTM databases

iPTMnetiP23771.
PhosphoSitePlusiP23771.

Expressioni

Tissue specificityi

T-cells and endothelial cells.

Gene expression databases

BgeeiENSG00000107485.
CleanExiHS_GATA3.
GenevisibleiP23771. HS.

Organism-specific databases

HPAiCAB016217.
HPA029730.
HPA029731.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
EHMT2Q96KQ720EBI-6664760,EBI-744366
MTA3Q9BTC818EBI-6664760,EBI-2461787
PSMA3P257883EBI-6664760,EBI-348380

GO - Molecular functioni

  • HMG box domain binding Source: UniProtKB
  • RNA polymerase II transcription factor binding Source: GO_Central
  • transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi108895. 21 interactors.
DIPiDIP-61302N.
IntActiP23771. 31 interactors.
MINTiMINT-4721003.
STRINGi9606.ENSP00000368632.

Structurei

Secondary structure

1443
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni264 – 266Combined sources3
Helixi285 – 294Combined sources10
Beta strandi307 – 313Combined sources7
Turni318 – 320Combined sources3
Helixi339 – 348Combined sources10
Helixi354 – 356Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HC7X-ray2.65A/B260-370[»]
4HC9X-ray1.60A260-370[»]
4HCAX-ray2.80A260-370[»]
ProteinModelPortaliP23771.
SMRiP23771.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni288 – 316Flexible linkerAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi344 – 353YxKxHxxxRPBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi137 – 142Poly-Ser6
Compositional biasi322 – 326Poly-Thr5

Domaini

Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation.
The YxKxHxxxRP motif is critical for DNA-binding and function.By similarity

Sequence similaritiesi

Contains 2 GATA-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri263 – 287GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri317 – 341GATA-type 2PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000047701.
HOVERGENiHBG051705.
InParanoidiP23771.
KOiK17895.
OMAiQRYPPTH.
OrthoDBiEOG091G0AUR.
PhylomeDBiP23771.
TreeFamiTF315391.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR029521. GATA-3.
IPR016374. TF_GATA-2/3.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF106. PTHR10071:SF106. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PIRSFiPIRSF003027. TF_GATA-1/2/3. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P23771-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVTADQPRW VSHHHPAVLN GQHPDTHHPG LSHSYMDAAQ YPLPEEVDVL
60 70 80 90 100
FNIDGQGNHV PPYYGNSVRA TVQRYPPTHH GSQVCRPPLL HGSLPWLDGG
110 120 130 140 150
KALGSHHTAS PWNLSPFSKT SIHHGSPGPL SVYPPASSSS LSGGHASPHL
160 170 180 190 200
FTFPPTPPKD VSPDPSLSTP GSAGSARQDE KECLKYQVPL PDSMKLESSH
210 220 230 240 250
SRGSMTALGG ASSSTHHPIT TYPPYVPEYS SGLFPPSSLL GGSPTGFGCK
260 270 280 290 300
SRPKARSSTG RECVNCGATS TPLWRRDGTG HYLCNACGLY HKMNGQNRPL
310 320 330 340 350
IKPKRRLSAA RRAGTSCANC QTTTTTLWRR NANGDPVCNA CGLYYKLHNI
360 370 380 390 400
NRPLTMKKEG IQTRNRKMSS KSKKCKKVHD SLEDFPKNSS FNPAALSRHM
410 420 430 440
SSLSHISPFS HSSHMLTTPT PMHPPSSLSF GPHHPSSMVT AMG
Length:443
Mass (Da):47,916
Last modified:November 1, 1991 - v1
Checksum:iF24C58681E1D02A5
GO
Isoform 2 (identifier: P23771-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     259-259: T → TE

Show »
Length:444
Mass (Da):48,045
Checksum:i506051438132F1D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11V → L in AAA35870 (PubMed:1871134).Curated1
Sequence conflicti102A → V in CAA38877 (PubMed:1827068).Curated1
Sequence conflicti173A → G in CAA38877 (PubMed:1827068).Curated1
Sequence conflicti219I → Y in AAA35870 (PubMed:1871134).Curated1
Sequence conflicti225 – 226YV → TC in AAA35870 (PubMed:1871134).Curated2
Sequence conflicti285 – 286NA → RR in AAA35870 (PubMed:1871134).Curated2
Sequence conflicti425P → A in AAA35870 (PubMed:1871134).Curated1
Sequence conflicti441A → G in AAA35870 (PubMed:1871134).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_019202242G → S.1 PublicationCorresponds to variant rs11567901dbSNPEnsembl.1
Natural variantiVAR_017818274W → R in HDR. 1 PublicationCorresponds to variant rs104894163dbSNPEnsembl.1
Natural variantiVAR_075427298R → Q in HDR; loss of enhancer activity on PTH gene promoter and on GATA responsive element. 1 Publication1
Natural variantiVAR_033025366R → L in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001598259T → TE in isoform 2. 3 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58072 mRNA. Translation: CAA41102.1.
X55037 mRNA. Translation: CAA38877.1.
X55122 mRNA. Translation: CAA38916.1.
M69106 mRNA. Translation: AAA35870.1.
AY497006 Genomic DNA. Translation: AAR32096.1.
AL390294 Genomic DNA. Translation: CAH73143.1.
AL390294 Genomic DNA. Translation: CAH73144.1.
CH471072 Genomic DNA. Translation: EAW86367.1.
CH471072 Genomic DNA. Translation: EAW86368.1.
BC003070 mRNA. Translation: AAH03070.1.
BC006793 mRNA. Translation: AAH06793.1.
CCDSiCCDS31143.1. [P23771-2]
CCDS7083.1. [P23771-1]
PIRiA39794.
RefSeqiNP_001002295.1. NM_001002295.1. [P23771-2]
NP_002042.1. NM_002051.2. [P23771-1]
XP_005252499.1. XM_005252442.2. [P23771-2]
XP_005252500.1. XM_005252443.4. [P23771-2]
UniGeneiHs.524134.

Genome annotation databases

EnsembliENST00000346208; ENSP00000341619; ENSG00000107485. [P23771-1]
ENST00000379328; ENSP00000368632; ENSG00000107485. [P23771-2]
GeneIDi2625.
KEGGihsa:2625.
UCSCiuc001ijz.4. human. [P23771-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

SeattleSNPs
Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58072 mRNA. Translation: CAA41102.1.
X55037 mRNA. Translation: CAA38877.1.
X55122 mRNA. Translation: CAA38916.1.
M69106 mRNA. Translation: AAA35870.1.
AY497006 Genomic DNA. Translation: AAR32096.1.
AL390294 Genomic DNA. Translation: CAH73143.1.
AL390294 Genomic DNA. Translation: CAH73144.1.
CH471072 Genomic DNA. Translation: EAW86367.1.
CH471072 Genomic DNA. Translation: EAW86368.1.
BC003070 mRNA. Translation: AAH03070.1.
BC006793 mRNA. Translation: AAH06793.1.
CCDSiCCDS31143.1. [P23771-2]
CCDS7083.1. [P23771-1]
PIRiA39794.
RefSeqiNP_001002295.1. NM_001002295.1. [P23771-2]
NP_002042.1. NM_002051.2. [P23771-1]
XP_005252499.1. XM_005252442.2. [P23771-2]
XP_005252500.1. XM_005252443.4. [P23771-2]
UniGeneiHs.524134.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HC7X-ray2.65A/B260-370[»]
4HC9X-ray1.60A260-370[»]
4HCAX-ray2.80A260-370[»]
ProteinModelPortaliP23771.
SMRiP23771.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108895. 21 interactors.
DIPiDIP-61302N.
IntActiP23771. 31 interactors.
MINTiMINT-4721003.
STRINGi9606.ENSP00000368632.

PTM databases

iPTMnetiP23771.
PhosphoSitePlusiP23771.

Polymorphism and mutation databases

BioMutaiGATA3.
DMDMi120962.

Proteomic databases

EPDiP23771.
MaxQBiP23771.
PaxDbiP23771.
PeptideAtlasiP23771.
PRIDEiP23771.

Protocols and materials databases

DNASUi2625.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000346208; ENSP00000341619; ENSG00000107485. [P23771-1]
ENST00000379328; ENSP00000368632; ENSG00000107485. [P23771-2]
GeneIDi2625.
KEGGihsa:2625.
UCSCiuc001ijz.4. human. [P23771-1]

Organism-specific databases

CTDi2625.
DisGeNETi2625.
GeneCardsiGATA3.
HGNCiHGNC:4172. GATA3.
HPAiCAB016217.
HPA029730.
HPA029731.
MalaCardsiGATA3.
MIMi131320. gene.
146255. phenotype.
neXtProtiNX_P23771.
OpenTargetsiENSG00000107485.
Orphaneti2237. Hypoparathyroidism - deafness - renal disease.
99860. Precursor B-cell acute lymphoblastic leukemia.
PharmGKBiPA28586.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000047701.
HOVERGENiHBG051705.
InParanoidiP23771.
KOiK17895.
OMAiQRYPPTH.
OrthoDBiEOG091G0AUR.
PhylomeDBiP23771.
TreeFamiTF315391.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000107485-MONOMER.
ReactomeiR-HSA-5689880. Ub-specific processing proteases.
R-HSA-983231. Factors involved in megakaryocyte development and platelet production.
SignaLinkiP23771.
SIGNORiP23771.

Miscellaneous databases

GeneWikiiGATA3.
GenomeRNAii2625.
PROiP23771.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000107485.
CleanExiHS_GATA3.
GenevisibleiP23771. HS.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR029521. GATA-3.
IPR016374. TF_GATA-2/3.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF106. PTHR10071:SF106. 1 hit.
PfamiPF00320. GATA. 2 hits.
[Graphical view]
PIRSFiPIRSF003027. TF_GATA-1/2/3. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA3_HUMAN
AccessioniPrimary (citable) accession number: P23771
Secondary accession number(s): Q5VWG7, Q5VWG8, Q96J16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 30, 2016
This is version 179 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.