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P23763 (VAMP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicle-associated membrane protein 1

Short name=VAMP-1
Alternative name(s):
Synaptobrevin-1
Gene names
Name:VAMP1
Synonyms:SYB1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length118 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the targeting and/or fusion of transport vesicles to their target membrane.

Subunit structure

Interacts with VAPA and VAPB.

Subcellular location

Isoform 1: Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Single-pass type IV membrane protein By similarity. Cell junctionsynapsesynaptosome By similarity Ref.2.

Isoform 2: Cytoplasmic vesicle membrane; Single-pass type IV membrane protein By similarity. Cell junctionsynapsesynaptosome By similarity Ref.2.

Isoform 3: Mitochondrion outer membrane; Single-pass type IV membrane protein Ref.2.

Tissue specificity

Nervous system, skeletal muscle and adipose tissue.

Sequence similarities

Belongs to the synaptobrevin family.

Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
   Cellular componentCell junction
Cytoplasmic vesicle
Membrane
Mitochondrion
Mitochondrion outer membrane
Synapse
Synaptosome
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
Transmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processneurotransmitter secretion

Traceable author statement. Source: Reactome

regulation of exocytosis

Inferred from Biological aspect of Ancestor. Source: RefGenome

synaptic transmission

Traceable author statement. Source: Reactome

vesicle fusion

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentSNARE complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

endocytic vesicle membrane

Traceable author statement. Source: Reactome

integral component of plasma membrane

Traceable author statement Ref.1. Source: ProtInc

mitochondrial outer membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

neuron projection

Inferred from electronic annotation. Source: UniProtKB-SubCell

synaptic vesicle

Inferred from Biological aspect of Ancestor. Source: RefGenome

synaptic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionSNAP receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

SNARE binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

protein binding

Inferred from physical interaction PubMed 9920726. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P23763-1)

Also known as: VAMP-1A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P23763-3)

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYFFT → SKYR
Isoform 3 (identifier: P23763-2)

Also known as: VAMP-1B;

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYFFT → RRD

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 118118Vesicle-associated membrane protein 1
PRO_0000206719

Regions

Topological domain1 – 9696Cytoplasmic Potential
Transmembrane97 – 11620Helical; Anchor for type IV membrane protein; Potential
Topological domain117 – 1182Vesicular Potential
Domain33 – 9361v-SNARE coiled-coil homology

Natural variations

Alternative sequence114 – 1185IYFFT → SKYR in isoform 2.
VSP_029185
Alternative sequence114 – 1185IYFFT → RRD in isoform 3.
VSP_006325

Experimental info

Sequence conflict1031A → T in CAA88760. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (VAMP-1A) [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 64CE9615447B686B

FASTA11812,902
        10         20         30         40         50         60 
MSAPAQPPAE GTEGTAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIIRV NVDKVLERDQ 

        70         80         90        100        110 
KLSELDDRAD ALQAGASQFE SSAAKLKRKY WWKNCKMMIM LGAICAIIVV VIVIYFFT 

« Hide

Isoform 2 [UniParc].

Checksum: F3449D319B686B1A
Show »

FASTA11712,765
Isoform 3 (VAMP-1B) [UniParc].

Checksum: 957A002B686B1AE6
Show »

FASTA11612,658

References

« Hide 'large scale' references
[1]"Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2."
Archer B.T. III, Oezcelik T., Jahn R., Francke U., Suedhof T.C.
J. Biol. Chem. 265:17267-17273(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[2]"A splice-isoform of vesicle-associated membrane protein-1 (VAMP-1) contains a mitochondrial targeting signal."
Isenmann S., Khew-Goodall Y., Gamble J., Vadas M., Wattenberg B.W.
Mol. Biol. Cell 9:1649-1660(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), SUBCELLULAR LOCATION.
Tissue: Umbilical vein.
[3]Gough K.H., Verkuylen A., Cosgrove L., Frenkel M.J., Ward C.W.
Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Skeletal muscle.
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Uterus.
[6]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M36200 expand/collapse EMBL AC list , M36196, M36197, M36198, M36199 Genomic DNA. Translation: AAA60603.1.
AF060538 mRNA. Translation: AAC28336.1.
Z48924 mRNA. Translation: CAA88760.1.
CR542214 mRNA. Translation: CAG47010.1.
CR542231 mRNA. Translation: CAG47027.1.
CR749485 mRNA. Translation: CAH18312.1.
AC005840 Genomic DNA. No translation available.
CH471116 Genomic DNA. Translation: EAW88792.1.
CH471116 Genomic DNA. Translation: EAW88795.1.
CH471116 Genomic DNA. Translation: EAW88796.1.
BC023286 mRNA. Translation: AAH23286.1.
CCDSCCDS31731.1. [P23763-3]
CCDS41740.1. [P23763-1]
CCDS44809.1. [P23763-2]
PIRA38315.
S52747.
RefSeqNP_055046.1. NM_014231.3. [P23763-1]
NP_058439.1. NM_016830.2. [P23763-2]
NP_954740.1. NM_199245.1. [P23763-3]
UniGeneHs.20021.

3D structure databases

ProteinModelPortalP23763.
SMRP23763. Positions 30-94.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112710. 7 interactions.
DIPDIP-873N.
STRING9606.ENSP00000380148.

Chemistry

DrugBankDB00042. Botulinum Toxin Type B.

Protein family/group databases

TCDB1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

PTM databases

PhosphoSiteP23763.

Polymorphism databases

DMDM135093.

Proteomic databases

PaxDbP23763.
PRIDEP23763.

Protocols and materials databases

DNASU6843.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361716; ENSP00000355122; ENSG00000139190. [P23763-3]
ENST00000396308; ENSP00000379602; ENSG00000139190. [P23763-1]
ENST00000400911; ENSP00000383702; ENSG00000139190. [P23763-2]
GeneID6843.
KEGGhsa:6843.
UCSCuc001qoj.3. human. [P23763-2]
uc001qok.3. human. [P23763-1]
uc001qol.3. human. [P23763-3]

Organism-specific databases

CTD6843.
GeneCardsGC12M006571.
HGNCHGNC:12642. VAMP1.
HPACAB002779.
CAB016119.
MIM185880. gene.
neXtProtNX_P23763.
Orphanet251282. Autosomal dominant spastic ataxia 1.
PharmGKBPA37266.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5143.
HOVERGENHBG006675.
InParanoidP23763.
KOK08510.
OMAWYLMSRR.
OrthoDBEOG7MSMRJ.
PhylomeDBP23763.
TreeFamTF313666.

Enzyme and pathway databases

ReactomeREACT_116125. Disease.

Gene expression databases

ArrayExpressP23763.
BgeeP23763.
CleanExHS_VAMP1.
GenevestigatorP23763.

Family and domain databases

InterProIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP_met/fun.
[Graphical view]
PfamPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSPR00219. SYNAPTOBREVN.
PROSITEPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiVAMP1.
GenomeRNAi6843.
NextBio26711.
PMAP-CutDBP23763.
PROP23763.
SOURCESearch...

Entry information

Entry nameVAMP1_HUMAN
AccessionPrimary (citable) accession number: P23763
Secondary accession number(s): A8MVP3 expand/collapse secondary AC list , D3DUR3, O75468, Q15857, Q6FG94, Q8IVC9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: July 9, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM