P23763 (VAMP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicle-associated membrane protein 1
Short name=VAMP-1

Alternative name(s):
Synaptobrevin-1

Gene names

Name:	VAMP1
Synonyms:	SYB1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Subunit structure	Interacts with VAPA and VAPB.
Subcellular location	Isoform 1: Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass type IV membrane protein By similarity. Cell junction › synapse › synaptosome By similarity Ref.2. Isoform 2: Cytoplasmic vesicle membrane; Single-pass type IV membrane protein By similarity. Cell junction › synapse › synaptosome By similarity Ref.2. Isoform 3: Mitochondrion outer membrane; Single-pass type IV membrane protein Ref.2.
Tissue specificity	Nervous system, skeletal muscle and adipose tissue.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Membrane Mitochondrion Mitochondrion outer membrane Synapse Synaptosome
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	neurotransmitter secretion Traceable author statement. Source: Reactome vesicle-mediated transport Inferred from electronic annotation. Source: InterPro
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW endocytic vesicle membrane Traceable author statement. Source: Reactome integral to plasma membrane Traceable author statement. Source: ProtInc mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synaptic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synaptosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P23763-1)

Also known as: VAMP-1A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P23763-3)

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYFFT → SKYR

Isoform 3 (identifier: P23763-2)

Also known as: VAMP-1B;

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYFFT → RRD

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	Vesicle-associated membrane protein 1		PRO_0000206719
Regions
	Topological domain	1 – 96	96	Cytoplasmic Potential
	Transmembrane	97 – 116	20	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	117 – 118	2	Vesicular Potential
	Domain	33 – 93	61	v-SNARE coiled-coil homology
Amino acid modifications
	Modified residue	63	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	114 – 118	5	IYFFT → SKYR in isoform 2.		VSP_029185
	Alternative sequence	114 – 118	5	IYFFT → RRD in isoform 3.		VSP_006325
Experimental info
	Sequence conflict	103	1	A → T in CAA88760. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (VAMP-1A) [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 64CE9615447B686B	FASTA	118	12,902
	10 20 30 40 50 60 MSAPAQPPAE GTEGTAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIIRV NVDKVLERDQ 70 80 90 100 110 KLSELDDRAD ALQAGASQFE SSAAKLKRKY WWKNCKMMIM LGAICAIIVV VIVIYFFT
	Isoform 2 [UniParc]. Checksum: F3449D319B686B1A	FASTA	117	12,765
	10 20 30 40 50 60 MSAPAQPPAE GTEGTAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIIRV NVDKVLERDQ 70 80 90 100 110 KLSELDDRAD ALQAGASQFE SSAAKLKRKY WWKNCKMMIM LGAICAIIVV VIVSKYR
	Isoform 3 (VAMP-1B) [UniParc]. Checksum: 957A002B686B1AE6	FASTA	116	12,658
	10 20 30 40 50 60 MSAPAQPPAE GTEGTAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIIRV NVDKVLERDQ 70 80 90 100 110 KLSELDDRAD ALQAGASQFE SSAAKLKRKY WWKNCKMMIM LGAICAIIVV VIVRRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2." Archer B.T. III, Oezcelik T., Jahn R., Francke U., Suedhof T.C. J. Biol. Chem. 265:17267-17273(1990) [PubMed: 1976629] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[2]	"A splice-isoform of vesicle-associated membrane protein-1 (VAMP-1) contains a mitochondrial targeting signal." Isenmann S., Khew-Goodall Y., Gamble J., Vadas M., Wattenberg B.W. Mol. Biol. Cell 9:1649-1660(1998) [PubMed: 9658161] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), SUBCELLULAR LOCATION. Tissue: Umbilical vein.
[3]	Gough K.H., Verkuylen A., Cosgrove L., Frenkel M.J., Ward C.W. Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Skeletal muscle.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Uterus.
[6]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed: 16541075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M36200 , M36196, M36197, M36198, M36199 Genomic DNA. Translation: AAA60603.1. AF060538 mRNA. Translation: AAC28336.1. Z48924 mRNA. Translation: CAA88760.1. CR542214 mRNA. Translation: CAG47010.1. CR542231 mRNA. Translation: CAG47027.1. CR749485 mRNA. Translation: CAH18312.1. AC005840 Genomic DNA. No translation available. CH471116 Genomic DNA. Translation: EAW88792.1. CH471116 Genomic DNA. Translation: EAW88795.1. CH471116 Genomic DNA. Translation: EAW88796.1. BC023286 mRNA. Translation: AAH23286.1.
IPI	IPI00012898. IPI00332864. IPI00397527.
PIR	A38315. S52747.
RefSeq	NP_055046.1. NM_014231.3. NP_058439.1. NM_016830.2. NP_954740.1. NM_199245.1.
UniGene	Hs.20021.
3D structure databases
ProteinModelPortal	P23763.
SMR	P23763. Positions 30-94.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-873N.
STRING	P23763.
Protein family/group databases
TCDB	1.F.1.1.1. synaptosomal vesicle fusion pore (SVF-Pore) family.
PTM databases
PhosphoSite	P23763.
Polymorphism databases
DMDM	135093.
Proteomic databases
PRIDE	P23763.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000396943; ENSP00000380148; ENSG00000139190.
GeneID	6843.
KEGG	hsa:6843.
UCSC	uc001qoj.1. human. uc001qok.1. human. uc001qol.1. human.
Organism-specific databases
CTD	6843.
GeneCards	GC12M006571.
HGNC	HGNC:12642. VAMP1.
HPA	CAB002779. CAB016119.
MIM	185880. gene.
neXtProt	NX_P23763.
PharmGKB	PA37266.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG20990.
GeneTree	ENSGT00550000074449.
HOVERGEN	HBG006675.
InParanoid	P23763.
PhylomeDB	P23763.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
ArrayExpress	P23763.
Bgee	P23763.
CleanEx	HS_VAMP1.
Genevestigator	P23763.
GermOnline	ENSG00000139190. Homo sapiens.
Family and domain databases
InterPro	IPR001388. Synaptobrevin. IPR016444. Synaptobrevin_met/fun. [Graphical view]
KO	K08510.
Pfam	PF00957. Synaptobrevin. 1 hit. [Graphical view]
PIRSF	PIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTS	PR00219. SYNAPTOBREVN.
PROSITE	PS00417. SYNAPTOBREVIN. 1 hit. PS50892. V_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00042. Botulinum Toxin Type B.
NextBio	26711.
PMAP-CutDB	P23763.
SOURCE	Search...

Entry information

Entry name

VAMP1_HUMAN

Accession

Primary (citable) accession number: P23763
Secondary accession number(s): A8MVP3 , D3DUR3, O75468, Q15857, Q6FG94, Q8IVC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	January 25, 2012
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.