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Protein

Vesicle-associated membrane protein 1

Gene

VAMP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_263857. Toxicity of botulinum toxin type F (BoNT/F).
REACT_263924. Toxicity of botulinum toxin type D (BoNT/D).
REACT_264271. Toxicity of botulinum toxin type G (BoNT/G).

Protein family/group databases

TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 1
Short name:
VAMP-1
Alternative name(s):
Synaptobrevin-1
Gene namesi
Name:VAMP1
Synonyms:SYB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:12642. VAMP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9696CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei97 – 11620Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini117 – 1182VesicularSequence Analysis

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cell surface Source: Ensembl
  • cytosol Source: Reactome
  • integral component of plasma membrane Source: ProtInc
  • mitochondrial outer membrane Source: UniProtKB-SubCell
  • SNARE complex Source: GO_Central
  • specific granule membrane Source: UniProtKB
  • synaptic vesicle Source: GO_Central
  • synaptic vesicle membrane Source: Reactome
  • terminal bouton Source: Ensembl
  • tertiary granule membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Mitochondrion, Mitochondrion outer membrane, Synapse, Synaptosome

Pathology & Biotechi

Involvement in diseasei

Spastic ataxia 1, autosomal dominant (SPAX1)1 Publication

The disease is caused by mutations affecting the gene represented in this entry. A mutation affecting a critical donor site for the splicing of VAMP1 isoforms leads to the loss of neuron-specific isoform 1 and subsequently results in haploinsufficiency (PubMed:22958904). Therefore, there would be less neurotransmitter exocytosis in specific regions of the brain, causing the symptoms of SPAX1.

Disease descriptionAn autosomal dominant form of spastic ataxia, a progressive neurodegenerative disorder characterized by lower-limb spasticity and generalized ataxia with dysarthria, impaired ocular movements, and gait disturbance.

See also OMIM:108600

Keywords - Diseasei

Neurodegeneration

Organism-specific databases

MIMi108600. phenotype.
Orphaneti251282. Autosomal dominant spastic ataxia type 1.
PharmGKBiPA37266.

Chemistry

DrugBankiDB00042. Botulinum Toxin Type B.

Polymorphism and mutation databases

BioMutaiVAMP1.
DMDMi135093.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 118118Vesicle-associated membrane protein 1PRO_0000206719Add
BLAST

Proteomic databases

PaxDbiP23763.
PRIDEiP23763.

PTM databases

PhosphoSiteiP23763.

Miscellaneous databases

PMAP-CutDBP23763.

Expressioni

Tissue specificityi

Nervous system, skeletal muscle and adipose tissue.

Gene expression databases

BgeeiP23763.
CleanExiHS_VAMP1.
ExpressionAtlasiP23763. baseline and differential.
GenevisibleiP23763. HS.

Organism-specific databases

HPAiCAB002779.
CAB016119.

Interactioni

Subunit structurei

Interacts with VAPA and VAPB.

Binary interactionsi

WithEntry#Exp.IntActNotes
CCDC155Q8N6L03EBI-10201335,EBI-749265
SNAP29O957213EBI-10201335,EBI-490676
STX4Q128463EBI-10201335,EBI-744942

Protein-protein interaction databases

BioGridi112710. 8 interactions.
DIPiDIP-873N.
IntActiP23763. 3 interactions.
STRINGi9606.ENSP00000379602.

Structurei

3D structure databases

ProteinModelPortaliP23763.
SMRiP23763. Positions 30-116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 9361v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5143.
GeneTreeiENSGT00550000074449.
HOVERGENiHBG006675.
InParanoidiP23763.
KOiK08510.
OMAiVIVIHEQ.
OrthoDBiEOG7MSMRJ.
PhylomeDBiP23763.
TreeFamiTF313666.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
IPR028715. VAMP1.
[Graphical view]
PANTHERiPTHR21136:SF32. PTHR21136:SF32. 1 hit.
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P23763-1) [UniParc]FASTAAdd to basket

Also known as: VAMP-1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAPAQPPAE GTEGTAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIIRV
60 70 80 90 100
NVDKVLERDQ KLSELDDRAD ALQAGASQFE SSAAKLKRKY WWKNCKMMIM
110
LGAICAIIVV VIVIYFFT
Length:118
Mass (Da):12,902
Last modified:November 1, 1991 - v1
Checksum:i64CE9615447B686B
GO
Isoform 2 (identifier: P23763-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYFFT → SKYR

Show »
Length:117
Mass (Da):12,765
Checksum:iF3449D319B686B1A
GO
Isoform 3 (identifier: P23763-2) [UniParc]FASTAAdd to basket

Also known as: VAMP-1B

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYFFT → RRD

Show »
Length:116
Mass (Da):12,658
Checksum:i957A002B686B1AE6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031A → T in CAA88760 (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei114 – 1185IYFFT → SKYR in isoform 2. 2 PublicationsVSP_029185
Alternative sequencei114 – 1185IYFFT → RRD in isoform 3. 2 PublicationsVSP_006325

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36200
, M36196, M36197, M36198, M36199 Genomic DNA. Translation: AAA60603.1.
AF060538 mRNA. Translation: AAC28336.1.
Z48924 mRNA. Translation: CAA88760.1.
CR542214 mRNA. Translation: CAG47010.1.
CR542231 mRNA. Translation: CAG47027.1.
CR749485 mRNA. Translation: CAH18312.1.
AC005840 Genomic DNA. No translation available.
CH471116 Genomic DNA. Translation: EAW88792.1.
CH471116 Genomic DNA. Translation: EAW88795.1.
CH471116 Genomic DNA. Translation: EAW88796.1.
BC023286 mRNA. Translation: AAH23286.1.
CCDSiCCDS31731.1. [P23763-3]
CCDS41740.1. [P23763-1]
CCDS44809.1. [P23763-2]
PIRiA38315.
S52747.
RefSeqiNP_001284367.1. NM_001297438.1.
NP_055046.1. NM_014231.4. [P23763-1]
NP_058439.1. NM_016830.3. [P23763-2]
NP_954740.1. NM_199245.2. [P23763-3]
UniGeneiHs.20021.

Genome annotation databases

EnsembliENST00000361716; ENSP00000355122; ENSG00000139190. [P23763-3]
ENST00000396308; ENSP00000379602; ENSG00000139190.
ENST00000400911; ENSP00000383702; ENSG00000139190. [P23763-2]
GeneIDi6843.
KEGGihsa:6843.
UCSCiuc001qoj.3. human. [P23763-2]
uc001qok.3. human. [P23763-1]
uc001qol.3. human. [P23763-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36200
, M36196, M36197, M36198, M36199 Genomic DNA. Translation: AAA60603.1.
AF060538 mRNA. Translation: AAC28336.1.
Z48924 mRNA. Translation: CAA88760.1.
CR542214 mRNA. Translation: CAG47010.1.
CR542231 mRNA. Translation: CAG47027.1.
CR749485 mRNA. Translation: CAH18312.1.
AC005840 Genomic DNA. No translation available.
CH471116 Genomic DNA. Translation: EAW88792.1.
CH471116 Genomic DNA. Translation: EAW88795.1.
CH471116 Genomic DNA. Translation: EAW88796.1.
BC023286 mRNA. Translation: AAH23286.1.
CCDSiCCDS31731.1. [P23763-3]
CCDS41740.1. [P23763-1]
CCDS44809.1. [P23763-2]
PIRiA38315.
S52747.
RefSeqiNP_001284367.1. NM_001297438.1.
NP_055046.1. NM_014231.4. [P23763-1]
NP_058439.1. NM_016830.3. [P23763-2]
NP_954740.1. NM_199245.2. [P23763-3]
UniGeneiHs.20021.

3D structure databases

ProteinModelPortaliP23763.
SMRiP23763. Positions 30-116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112710. 8 interactions.
DIPiDIP-873N.
IntActiP23763. 3 interactions.
STRINGi9606.ENSP00000379602.

Chemistry

DrugBankiDB00042. Botulinum Toxin Type B.

Protein family/group databases

TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

PTM databases

PhosphoSiteiP23763.

Polymorphism and mutation databases

BioMutaiVAMP1.
DMDMi135093.

Proteomic databases

PaxDbiP23763.
PRIDEiP23763.

Protocols and materials databases

DNASUi6843.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361716; ENSP00000355122; ENSG00000139190. [P23763-3]
ENST00000396308; ENSP00000379602; ENSG00000139190.
ENST00000400911; ENSP00000383702; ENSG00000139190. [P23763-2]
GeneIDi6843.
KEGGihsa:6843.
UCSCiuc001qoj.3. human. [P23763-2]
uc001qok.3. human. [P23763-1]
uc001qol.3. human. [P23763-3]

Organism-specific databases

CTDi6843.
GeneCardsiGC12M006571.
HGNCiHGNC:12642. VAMP1.
HPAiCAB002779.
CAB016119.
MIMi108600. phenotype.
185880. gene.
neXtProtiNX_P23763.
Orphaneti251282. Autosomal dominant spastic ataxia type 1.
PharmGKBiPA37266.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5143.
GeneTreeiENSGT00550000074449.
HOVERGENiHBG006675.
InParanoidiP23763.
KOiK08510.
OMAiVIVIHEQ.
OrthoDBiEOG7MSMRJ.
PhylomeDBiP23763.
TreeFamiTF313666.

Enzyme and pathway databases

ReactomeiREACT_263857. Toxicity of botulinum toxin type F (BoNT/F).
REACT_263924. Toxicity of botulinum toxin type D (BoNT/D).
REACT_264271. Toxicity of botulinum toxin type G (BoNT/G).

Miscellaneous databases

GeneWikiiVAMP1.
GenomeRNAii6843.
NextBioi26711.
PMAP-CutDBP23763.
PROiP23763.
SOURCEiSearch...

Gene expression databases

BgeeiP23763.
CleanExiHS_VAMP1.
ExpressionAtlasiP23763. baseline and differential.
GenevisibleiP23763. HS.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
IPR028715. VAMP1.
[Graphical view]
PANTHERiPTHR21136:SF32. PTHR21136:SF32. 1 hit.
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2."
    Archer B.T. III, Oezcelik T., Jahn R., Francke U., Suedhof T.C.
    J. Biol. Chem. 265:17267-17273(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
  2. "A splice-isoform of vesicle-associated membrane protein-1 (VAMP-1) contains a mitochondrial targeting signal."
    Isenmann S., Khew-Goodall Y., Gamble J., Vadas M., Wattenberg B.W.
    Mol. Biol. Cell 9:1649-1660(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), SUBCELLULAR LOCATION.
    Tissue: Umbilical vein.
  3. Gough K.H., Verkuylen A., Cosgrove L., Frenkel M.J., Ward C.W.
    Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Skeletal muscle.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Uterus.
  6. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  9. Cited for: INVOLVEMENT IN SPAX1.

Entry informationi

Entry nameiVAMP1_HUMAN
AccessioniPrimary (citable) accession number: P23763
Secondary accession number(s): A8MVP3
, D3DUR3, O75468, Q15857, Q6FG94, Q8IVC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: July 22, 2015
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.