P23756 (RBS_GALSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribulose bisphosphate carboxylase small chain
Short name=RuBisCO small subunit
EC=4.1.1.39

Gene names

Name:

rbcS

Encoded on

Plastid; Chloroplast

Organism

Galdieria sulphuraria (Red alga)

Taxonomic identifier

130081 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Rhodophyta › Bangiophyceae › Cyanidiales › Cyanidiaceae › Galdieria

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
Subunit structure	8 large chains + 8 small chains.
Subcellular location	Plastid › chloroplast.
Miscellaneous	In this alga, in contrast to plants, the small subunit is encoded in the chloroplast.
Sequence similarities	Belongs to the RuBisCO small chain family.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation Photorespiration Photosynthesis
Cellular component	Chloroplast Plastid
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	photorespiration Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 138

138

Ribulose bisphosphate carboxylase small chain

PRO_0000198598

Secondary structure

1

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138

Helix Strand Turn

Details...

Turn

7 – 10

4

Helix

16 – 28

13

Beta strand

32 – 39

8

Beta strand

49 – 52

4

Helix

61 – 74

14

Turn

75 – 77

3

Beta strand

78 – 86

9

Turn

89 – 91

3

Beta strand

93 – 101

9

Beta strand

109 – 115

7

Beta strand

121 – 127

7

Helix

128 – 131

4

Helix

134 – 136

3

Sequences

	Sequence		Length	Mass (Da)
	P23756 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 5E946BA51DAFAEC2	FASTA	138	16,206
	10 20 30 40 50 60 MRITQGTFSF LPDLTDEQIK KQIDYMISKK LAIGIEYTND IHPRNSFWEM WGLPLFEVTD 70 80 90 100 110 120 PAPVLFEINA CRKAKSNFYI KVVGFSSERG IESTIISFIV NRPKHEPGFN LIRQEDKSRS 130 IKYSIQAYET YKPEDQRY

References

[1]	"Structure of the Rubisco operon from the unicellular red alga Cyanidium caldarium: evidence for a polyphyletic origin of the plastids." Valentin K.-U., Zetsche K. Mol. Gen. Genet. 222:425-430(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 14-1-1 / Isolate 107.79/Goettingen.
[2]	Whitney S.M., Andrews J. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: UTEX 2393.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X55524 Genomic DNA. Translation: CAA39141.2.
AF233069 Genomic DNA. Translation: AAF81682.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4F0H	X-ray	1.96	B	1-138	[»]
4F0K	X-ray	2.05	B	1-138	[»]
4F0M	X-ray	2.25	B	1-138	[»]

ProteinModelPortal

P23756.

SMR

P23756. Positions 1-138.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.190.10. 1 hit.

InterPro

IPR000894. RuBisCO_sc_dom.
[Graphical view]

Pfam

PF00101. RuBisCO_small. 1 hit.
[Graphical view]

SUPFAM

SSF55239. RuBisCO_small. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RBS_GALSU

Accession

Primary (citable) accession number: P23756

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)