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P23755

- RBL_GALSU

UniProt

P23755 - RBL_GALSU

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Protein
Ribulose bisphosphate carboxylase large chain
Gene
rbcL
Organism
Galdieria sulphuraria (Red alga)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site By similarity.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei132 – 1321Substrate; in homodimeric partner By similarity
Binding sitei182 – 1821Substrate By similarity
Active sitei184 – 1841Proton acceptor By similarity
Binding sitei186 – 1861Substrate By similarity
Metal bindingi210 – 2101Magnesium; via carbamate group By similarity
Metal bindingi212 – 2121Magnesium By similarity
Metal bindingi213 – 2131Magnesium By similarity
Active sitei302 – 3021Proton acceptor By similarity
Binding sitei303 – 3031Substrate By similarity
Binding sitei335 – 3351Substrate By similarity
Sitei342 – 3421Transition state stabilizer By similarity
Binding sitei387 – 3871Substrate By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. monooxygenase activity Source: UniProtKB-KW
  3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. photorespiration Source: UniProtKB-KW
  2. reductive pentose-phosphate cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Calvin cycle, Carbon dioxide fixation, Photorespiration, Photosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chain (EC:4.1.1.39)
Short name:
RuBisCO large subunit
Gene namesi
Name:rbcL
Encoded oniPlastid; Chloroplast
OrganismiGaldieria sulphuraria (Red alga)
Taxonomic identifieri130081 [NCBI]
Taxonomic lineageiEukaryotaRhodophytaBangiophyceaeCyanidialesCyanidiaceaeGaldieria

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Ribulose bisphosphate carboxylase large chainUniRule annotation
PRO_0000062476Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei210 – 2101N6-carboxylysine By similarity

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains By similarity.

Protein-protein interaction databases

DIPiDIP-60089N.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 335
Beta strandi45 – 539
Helixi59 – 6911
Helixi79 – 835
Helixi86 – 894
Beta strandi92 – 998
Beta strandi102 – 11211
Helixi114 – 1163
Helixi122 – 1309
Helixi133 – 1353
Turni136 – 1383
Beta strandi139 – 14810
Helixi151 – 1544
Helixi163 – 1719
Beta strandi178 – 1825
Beta strandi184 – 1874
Helixi191 – 20414
Beta strandi207 – 2104
Beta strandi216 – 2183
Helixi223 – 24119
Beta strandi246 – 2505
Helixi256 – 26914
Beta strandi272 – 2776
Helixi278 – 2803
Helixi282 – 29413
Beta strandi298 – 3025
Turni304 – 3063
Helixi307 – 3104
Beta strandi311 – 3177
Helixi319 – 32911
Beta strandi332 – 3354
Beta strandi339 – 3413
Helixi347 – 35812
Beta strandi360 – 3623
Turni366 – 3694
Beta strandi383 – 3864
Helixi392 – 3943
Helixi395 – 4028
Beta strandi407 – 4115
Helixi412 – 4154
Helixi421 – 44020
Helixi445 – 45814
Helixi461 – 4699
Turni470 – 4734

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4F0HX-ray1.96A1-493[»]
4F0KX-ray2.05A1-493[»]
4F0MX-ray2.25A1-493[»]
ProteinModelPortaliP23755.
SMRiP23755. Positions 14-486.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1.
InterProiIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23755-1 [UniParc]FASTAAdd to Basket

« Hide

MSQSLEEKSV QERTRIKNSR YESGVIPYAK MGYWNPDYQV KDTDVLALFR    50
VTPQPGVDPI EAAAAVAGES STATWTVVWT DLLTAADLYR AKAYKVDQVP 100
NNPEQYFAYI AYELDLFEEG SIANLTASII GNVFGFKAVK ALRLEDMRLP 150
FAYIKTFQGP ATGVILERER LDKFGRPLLG CTTKPKLGLS GKNYGRVVYE 200
ALKGGLDFVK DDENINSQPF MRWRERYLFV MEAVNKAAAA TGEVKGHYLN 250
VTAATMEEMY ARAQLAKELG SVIIMIDLVI GYTAIQTMAK WARDNDMILH 300
LHRAGNSTYS RQKNHGMNFR VICKWMRMAG VDHIHAGTVV GKLEGDPIIT 350
RGFYKTLLLP KLERNLQEGL FFDMDWASLR KVMPVASGGI HAGQMHQLIH 400
YLGEDVVLQF GGGTIGHPDG IQSGATANRV ALEAMILARN ENRDFLTEGP 450
EILREAAKNC GALRTALDLW KDITFNYTST DTSDFVETPT ANI 493
Length:493
Mass (Da):54,989
Last modified:November 1, 1991 - v1
Checksum:iB69EE280D47C6FA2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55524 Genomic DNA. Translation: CAA39140.1.
AF233069 Genomic DNA. Translation: AAF81681.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55524 Genomic DNA. Translation: CAA39140.1 .
AF233069 Genomic DNA. Translation: AAF81681.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4F0H X-ray 1.96 A 1-493 [» ]
4F0K X-ray 2.05 A 1-493 [» ]
4F0M X-ray 2.25 A 1-493 [» ]
ProteinModelPortali P23755.
SMRi P23755. Positions 14-486.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-60089N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPi MF_01338. RuBisCO_L_type1.
InterProi IPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view ]
Pfami PF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEi PS00157. RUBISCO_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure of the Rubisco operon from the unicellular red alga Cyanidium caldarium: evidence for a polyphyletic origin of the plastids."
    Valentin K.-U., Zetsche K.
    Mol. Gen. Genet. 222:425-430(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 14-1-1 / Isolate 107.79/Goettingen.
  2. Whitney S.M., Andrews J.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: UTEX 2393.

Entry informationi

Entry nameiRBL_GALSU
AccessioniPrimary (citable) accession number: P23755
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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