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P23748

- MPIP_YEAST

UniProt

P23748 - MPIP_YEAST

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Protein
M-phase inducer phosphatase
Gene
MIH1, YMR036C, YM9532.01C, YM9973.10C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Terminates the cell cycle delay. Reverses the CDC28 phosphorylation catalyzed by SWE1.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei320 – 3201 By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. G2/M transition of mitotic cell cycle Source: SGD
  2. mitotic nuclear division Source: UniProtKB-KW
  3. peptidyl-tyrosine dephosphorylation Source: GOC
  4. regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32741-MONOMER.
ReactomeiREACT_188487. Cyclin A/B1 associated events during G2/M transition.
REACT_201908. Activation of ATR in response to replication stress.
REACT_205363. Cyclin B2 mediated events.
REACT_211772. E2F-enabled inhibition of pre-replication complex formation.
REACT_220678. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_224255. Cyclin A:Cdk2-associated events at S phase entry.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase (EC:3.1.3.48)
Alternative name(s):
Mitosis initiation protein MIH1
Mitotic inducer homolog
Gene namesi
Name:MIH1
Ordered Locus Names:YMR036C
ORF Names:YM9532.01C, YM9973.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR036c.
SGDiS000004639. MIH1.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554M-phase inducer phosphatase
PRO_0000198662Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP23748.

Interactioni

Protein-protein interaction databases

BioGridi35208. 66 interactions.
DIPiDIP-2713N.
IntActiP23748. 15 interactions.
MINTiMINT-678695.
STRINGi4932.YMR036C.

Structurei

3D structure databases

ProteinModelPortaliP23748.
SMRiP23748. Positions 244-410.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini261 – 373113Rhodanese
Add
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.

Phylogenomic databases

eggNOGiCOG5105.
GeneTreeiENSGT00390000018747.
KOiK02555.
OMAiTEDECAN.
OrthoDBiEOG7VMPGR.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23748-1 [UniParc]FASTAAdd to Basket

« Hide

MNNIFHGTED ECANEDVLSF QKISLKSPFG KKKNIFRNVQ TFFKSKSKHS    50
NVDDDLINKE NLAFDKSPLL TNHRSKEIDG PSPNIKQLGH RDELDENENE 100
NDDIVLSMHF ASQTLQSPTR NSSRRSLTNN RDNDLLSRIK YPGSPQRSSS 150
FSRSRSLSRK PSMNSSSNSS RRVQRQDGKI PRSSRKSSQK FSNITQNTLN 200
FTSASSSPLA PNSVGVKCFE SCLAKTQIPY YYDDRNSNDF FPRISPETLK 250
NILQNNMCES FYNSCRIIDC RFEYEYTGGH IINSVNIHSR DELEYEFIHK 300
VLHSDTSNNN TLPTLLIIHC EFSSHRGPSL ASHLRNCDRI INQDHYPKLF 350
YPDILILDGG YKAVFDNFPE LCYPRQYVGM NSQENLLNCE QEMDKFRRES 400
KRFATKNNSF RKLASPSNPN FFYRDSHQSS TTMASSALSF RFEPPPKLSL 450
NHRRVSSGSS LNSSESTGDE NFFPILSKSS MSSNSNLSTS HMLLMDGLDT 500
PSYFSFEDER GNHQQVSGDE EQDGDFTFVG SDREDLPRPA RRSLFPSLET 550
EDKK 554
Length:554
Mass (Da):63,358
Last modified:November 1, 1995 - v2
Checksum:iDBD85D02CF48CA6F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti238 – 2392ND → MT in AAA57223. 1 Publication
Sequence conflicti241 – 2488FPRISPET → SLEFLQKR in AAA57223. 1 Publication
Sequence conflicti365 – 3673FDN → LT in AAA57223. 1 Publication
Sequence conflicti475 – 55480ILSKS…TEDKK → S in AAA57223. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04846 Genomic DNA. Translation: AAA57223.1.
Z48502 Genomic DNA. Translation: CAA88402.1.
Z49213 Genomic DNA. Translation: CAA89152.1.
BK006946 Genomic DNA. Translation: DAA09935.1.
PIRiA32386.
RefSeqiNP_013750.1. NM_001182533.1.

Genome annotation databases

EnsemblFungiiYMR036C; YMR036C; YMR036C.
GeneIDi855052.
KEGGisce:YMR036C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04846 Genomic DNA. Translation: AAA57223.1 .
Z48502 Genomic DNA. Translation: CAA88402.1 .
Z49213 Genomic DNA. Translation: CAA89152.1 .
BK006946 Genomic DNA. Translation: DAA09935.1 .
PIRi A32386.
RefSeqi NP_013750.1. NM_001182533.1.

3D structure databases

ProteinModelPortali P23748.
SMRi P23748. Positions 244-410.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35208. 66 interactions.
DIPi DIP-2713N.
IntActi P23748. 15 interactions.
MINTi MINT-678695.
STRINGi 4932.YMR036C.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR036C ; YMR036C ; YMR036C .
GeneIDi 855052.
KEGGi sce:YMR036C.

Organism-specific databases

CYGDi YMR036c.
SGDi S000004639. MIH1.

Phylogenomic databases

eggNOGi COG5105.
GeneTreei ENSGT00390000018747.
KOi K02555.
OMAi TEDECAN.
OrthoDBi EOG7VMPGR.

Enzyme and pathway databases

BioCyci YEAST:G3O-32741-MONOMER.
Reactomei REACT_188487. Cyclin A/B1 associated events during G2/M transition.
REACT_201908. Activation of ATR in response to replication stress.
REACT_205363. Cyclin B2 mediated events.
REACT_211772. E2F-enabled inhibition of pre-replication complex formation.
REACT_220678. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_224255. Cyclin A:Cdk2-associated events at S phase entry.

Miscellaneous databases

NextBioi 978292.
PROi P23748.

Gene expression databases

Genevestigatori P23748.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view ]
Pfami PF00581. Rhodanese. 1 hit.
[Graphical view ]
PRINTSi PR00716. MPIPHPHTASE.
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Conservation of mitotic controls in fission and budding yeasts."
    Russell P., Moreno S., Reed S.I.
    Cell 57:295-303(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMPIP_YEAST
AccessioniPrimary (citable) accession number: P23748
Secondary accession number(s): D6VZL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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