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P23748

- MPIP_YEAST

UniProt

P23748 - MPIP_YEAST

Protein

M-phase inducer phosphatase

Gene

MIH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Terminates the cell cycle delay. Reverses the CDC28 phosphorylation catalyzed by SWE1.

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei320 – 3201By similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: SGD

    GO - Biological processi

    1. G2/M transition of mitotic cell cycle Source: SGD
    2. mitotic nuclear division Source: UniProtKB-KW
    3. peptidyl-tyrosine dephosphorylation Source: GOC
    4. regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32741-MONOMER.
    ReactomeiREACT_188487. Cyclin A/B1 associated events during G2/M transition.
    REACT_201908. Activation of ATR in response to replication stress.
    REACT_205363. Cyclin B2 mediated events.
    REACT_211772. E2F-enabled inhibition of pre-replication complex formation.
    REACT_220678. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_224255. Cyclin A:Cdk2-associated events at S phase entry.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    M-phase inducer phosphatase (EC:3.1.3.48)
    Alternative name(s):
    Mitosis initiation protein MIH1
    Mitotic inducer homolog
    Gene namesi
    Name:MIH1
    Ordered Locus Names:YMR036C
    ORF Names:YM9532.01C, YM9973.10C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR036c.
    SGDiS000004639. MIH1.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 554554M-phase inducer phosphatasePRO_0000198662Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP23748.

    Interactioni

    Protein-protein interaction databases

    BioGridi35208. 66 interactions.
    DIPiDIP-2713N.
    IntActiP23748. 15 interactions.
    MINTiMINT-678695.
    STRINGi4932.YMR036C.

    Structurei

    3D structure databases

    ProteinModelPortaliP23748.
    SMRiP23748. Positions 244-410.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini261 – 373113RhodanesePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MPI phosphatase family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5105.
    GeneTreeiENSGT00390000018747.
    KOiK02555.
    OMAiTEDECAN.
    OrthoDBiEOG7VMPGR.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    [Graphical view]
    PRINTSiPR00716. MPIPHPHTASE.
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23748-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNIFHGTED ECANEDVLSF QKISLKSPFG KKKNIFRNVQ TFFKSKSKHS    50
    NVDDDLINKE NLAFDKSPLL TNHRSKEIDG PSPNIKQLGH RDELDENENE 100
    NDDIVLSMHF ASQTLQSPTR NSSRRSLTNN RDNDLLSRIK YPGSPQRSSS 150
    FSRSRSLSRK PSMNSSSNSS RRVQRQDGKI PRSSRKSSQK FSNITQNTLN 200
    FTSASSSPLA PNSVGVKCFE SCLAKTQIPY YYDDRNSNDF FPRISPETLK 250
    NILQNNMCES FYNSCRIIDC RFEYEYTGGH IINSVNIHSR DELEYEFIHK 300
    VLHSDTSNNN TLPTLLIIHC EFSSHRGPSL ASHLRNCDRI INQDHYPKLF 350
    YPDILILDGG YKAVFDNFPE LCYPRQYVGM NSQENLLNCE QEMDKFRRES 400
    KRFATKNNSF RKLASPSNPN FFYRDSHQSS TTMASSALSF RFEPPPKLSL 450
    NHRRVSSGSS LNSSESTGDE NFFPILSKSS MSSNSNLSTS HMLLMDGLDT 500
    PSYFSFEDER GNHQQVSGDE EQDGDFTFVG SDREDLPRPA RRSLFPSLET 550
    EDKK 554
    Length:554
    Mass (Da):63,358
    Last modified:November 1, 1995 - v2
    Checksum:iDBD85D02CF48CA6F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti238 – 2392ND → MT in AAA57223. (PubMed:2649252)Curated
    Sequence conflicti241 – 2488FPRISPET → SLEFLQKR in AAA57223. (PubMed:2649252)Curated
    Sequence conflicti365 – 3673FDN → LT in AAA57223. (PubMed:2649252)Curated
    Sequence conflicti475 – 55480ILSKS…TEDKK → S in AAA57223. (PubMed:2649252)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04846 Genomic DNA. Translation: AAA57223.1.
    Z48502 Genomic DNA. Translation: CAA88402.1.
    Z49213 Genomic DNA. Translation: CAA89152.1.
    BK006946 Genomic DNA. Translation: DAA09935.1.
    PIRiA32386.
    RefSeqiNP_013750.1. NM_001182533.1.

    Genome annotation databases

    EnsemblFungiiYMR036C; YMR036C; YMR036C.
    GeneIDi855052.
    KEGGisce:YMR036C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04846 Genomic DNA. Translation: AAA57223.1 .
    Z48502 Genomic DNA. Translation: CAA88402.1 .
    Z49213 Genomic DNA. Translation: CAA89152.1 .
    BK006946 Genomic DNA. Translation: DAA09935.1 .
    PIRi A32386.
    RefSeqi NP_013750.1. NM_001182533.1.

    3D structure databases

    ProteinModelPortali P23748.
    SMRi P23748. Positions 244-410.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35208. 66 interactions.
    DIPi DIP-2713N.
    IntActi P23748. 15 interactions.
    MINTi MINT-678695.
    STRINGi 4932.YMR036C.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR036C ; YMR036C ; YMR036C .
    GeneIDi 855052.
    KEGGi sce:YMR036C.

    Organism-specific databases

    CYGDi YMR036c.
    SGDi S000004639. MIH1.

    Phylogenomic databases

    eggNOGi COG5105.
    GeneTreei ENSGT00390000018747.
    KOi K02555.
    OMAi TEDECAN.
    OrthoDBi EOG7VMPGR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32741-MONOMER.
    Reactomei REACT_188487. Cyclin A/B1 associated events during G2/M transition.
    REACT_201908. Activation of ATR in response to replication stress.
    REACT_205363. Cyclin B2 mediated events.
    REACT_211772. E2F-enabled inhibition of pre-replication complex formation.
    REACT_220678. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_224255. Cyclin A:Cdk2-associated events at S phase entry.

    Miscellaneous databases

    NextBioi 978292.
    PROi P23748.

    Gene expression databases

    Genevestigatori P23748.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view ]
    Pfami PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PRINTSi PR00716. MPIPHPHTASE.
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Conservation of mitotic controls in fission and budding yeasts."
      Russell P., Moreno S., Reed S.I.
      Cell 57:295-303(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMPIP_YEAST
    AccessioniPrimary (citable) accession number: P23748
    Secondary accession number(s): D6VZL1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3