Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P23748 (MPIP_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
M-phase inducer phosphatase

EC=3.1.3.48
Alternative name(s):
Mitosis initiation protein MIH1
Mitotic inducer homolog
Gene names
Name:MIH1
Ordered Locus Names:YMR036C
ORF Names:YM9532.01C, YM9973.10C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Terminates the cell cycle delay. Reverses the CDC28 phosphorylation catalyzed by SWE1.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sequence similarities

Belongs to the MPI phosphatase family.

Contains 1 rhodanese domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554M-phase inducer phosphatase
PRO_0000198662

Regions

Domain261 – 373113Rhodanese

Sites

Active site3201 By similarity

Experimental info

Sequence conflict238 – 2392ND → MT in AAA57223. Ref.1
Sequence conflict241 – 2488FPRISPET → SLEFLQKR in AAA57223. Ref.1
Sequence conflict365 – 3673FDN → LT in AAA57223. Ref.1
Sequence conflict475 – 55480ILSKS…TEDKK → S in AAA57223. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P23748 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: DBD85D02CF48CA6F

FASTA55463,358
        10         20         30         40         50         60 
MNNIFHGTED ECANEDVLSF QKISLKSPFG KKKNIFRNVQ TFFKSKSKHS NVDDDLINKE 

        70         80         90        100        110        120 
NLAFDKSPLL TNHRSKEIDG PSPNIKQLGH RDELDENENE NDDIVLSMHF ASQTLQSPTR 

       130        140        150        160        170        180 
NSSRRSLTNN RDNDLLSRIK YPGSPQRSSS FSRSRSLSRK PSMNSSSNSS RRVQRQDGKI 

       190        200        210        220        230        240 
PRSSRKSSQK FSNITQNTLN FTSASSSPLA PNSVGVKCFE SCLAKTQIPY YYDDRNSNDF 

       250        260        270        280        290        300 
FPRISPETLK NILQNNMCES FYNSCRIIDC RFEYEYTGGH IINSVNIHSR DELEYEFIHK 

       310        320        330        340        350        360 
VLHSDTSNNN TLPTLLIIHC EFSSHRGPSL ASHLRNCDRI INQDHYPKLF YPDILILDGG 

       370        380        390        400        410        420 
YKAVFDNFPE LCYPRQYVGM NSQENLLNCE QEMDKFRRES KRFATKNNSF RKLASPSNPN 

       430        440        450        460        470        480 
FFYRDSHQSS TTMASSALSF RFEPPPKLSL NHRRVSSGSS LNSSESTGDE NFFPILSKSS 

       490        500        510        520        530        540 
MSSNSNLSTS HMLLMDGLDT PSYFSFEDER GNHQQVSGDE EQDGDFTFVG SDREDLPRPA 

       550 
RRSLFPSLET EDKK 

« Hide

References

« Hide 'large scale' references
[1]"Conservation of mitotic controls in fission and budding yeasts."
Russell P., Moreno S., Reed S.I.
Cell 57:295-303(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04846 Genomic DNA. Translation: AAA57223.1.
Z48502 Genomic DNA. Translation: CAA88402.1.
Z49213 Genomic DNA. Translation: CAA89152.1.
BK006946 Genomic DNA. Translation: DAA09935.1.
PIRA32386.
RefSeqNP_013750.1. NM_001182533.1.

3D structure databases

ProteinModelPortalP23748.
SMRP23748. Positions 244-410.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35208. 66 interactions.
DIPDIP-2713N.
IntActP23748. 15 interactions.
MINTMINT-678695.
STRING4932.YMR036C.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR036C; YMR036C; YMR036C.
GeneID855052.
KEGGsce:YMR036C.

Organism-specific databases

CYGDYMR036c.
SGDS000004639. MIH1.

Phylogenomic databases

eggNOGCOG5105.
GeneTreeENSGT00390000018747.
KOK02555.
OMATEDECAN.
OrthoDBEOG7VMPGR.

Enzyme and pathway databases

BioCycYEAST:G3O-32741-MONOMER.

Gene expression databases

GenevestigatorP23748.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
InterProIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSPR00716. MPIPHPHTASE.
SMARTSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52821. SSF52821. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978292.
PROP23748.

Entry information

Entry nameMPIP_YEAST
AccessionPrimary (citable) accession number: P23748
Secondary accession number(s): D6VZL1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families