P23748 (MPIP_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
M-phase inducer phosphatase
EC=3.1.3.48

Alternative name(s):
Mitosis initiation protein MIH1
Mitotic inducer homolog

Gene names

Name:	MIH1
Ordered Locus Names:	YMR036C
ORF Names:	YM9532.01C, YM9973.10C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Terminates the cell cycle delay. Reverses the CDC28 phosphorylation catalyzed by SWE1.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Sequence similarities	Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G2/M transition of mitotic cell cycle Inferred from genetic interaction Ref.1. Source: SGD M phase of mitotic cell cycle Inferred from electronic annotation. Source: InterPro cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	protein tyrosine phosphatase activity Inferred from sequence or structural similarity Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	M-phase inducer phosphatase		PRO_0000198662
Regions
	Domain	261 – 373	113	Rhodanese
Sites
	Active site	320	1	By similarity
Amino acid modifications
	Modified residue	382	1	Phosphoserine Ref.5
	Modified residue	417	1	Phosphoserine Ref.4 Ref.5
Experimental info
	Sequence conflict	238 – 239	2	ND → MT in AAA57223. Ref.1
	Sequence conflict	241 – 248	8	FPRISPET → SLEFLQKR in AAA57223. Ref.1
	Sequence conflict	365 – 367	3	FDN → LT in AAA57223. Ref.1
	Sequence conflict	475 – 554	80	ILSKS…TEDKK → S in AAA57223. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P23748 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: DBD85D02CF48CA6F	FASTA	554	63,358
	10 20 30 40 50 60 MNNIFHGTED ECANEDVLSF QKISLKSPFG KKKNIFRNVQ TFFKSKSKHS NVDDDLINKE 70 80 90 100 110 120 NLAFDKSPLL TNHRSKEIDG PSPNIKQLGH RDELDENENE NDDIVLSMHF ASQTLQSPTR 130 140 150 160 170 180 NSSRRSLTNN RDNDLLSRIK YPGSPQRSSS FSRSRSLSRK PSMNSSSNSS RRVQRQDGKI 190 200 210 220 230 240 PRSSRKSSQK FSNITQNTLN FTSASSSPLA PNSVGVKCFE SCLAKTQIPY YYDDRNSNDF 250 260 270 280 290 300 FPRISPETLK NILQNNMCES FYNSCRIIDC RFEYEYTGGH IINSVNIHSR DELEYEFIHK 310 320 330 340 350 360 VLHSDTSNNN TLPTLLIIHC EFSSHRGPSL ASHLRNCDRI INQDHYPKLF YPDILILDGG 370 380 390 400 410 420 YKAVFDNFPE LCYPRQYVGM NSQENLLNCE QEMDKFRRES KRFATKNNSF RKLASPSNPN 430 440 450 460 470 480 FFYRDSHQSS TTMASSALSF RFEPPPKLSL NHRRVSSGSS LNSSESTGDE NFFPILSKSS 490 500 510 520 530 540 MSSNSNLSTS HMLLMDGLDT PSYFSFEDER GNHQQVSGDE EQDGDFTFVG SDREDLPRPA 550 RRSLFPSLET EDKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Conservation of mitotic controls in fission and budding yeasts." Russell P., Moreno S., Reed S.I. Cell 57:295-303(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417, MASS SPECTROMETRY.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-382 AND SER-417, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04846 Genomic DNA. Translation: AAA57223.1. Z48502 Genomic DNA. Translation: CAA88402.1. Z49213 Genomic DNA. Translation: CAA89152.1. BK006946 Genomic DNA. Translation: DAA09935.1.
PIR	A32386.
RefSeq	NP_013750.1. NM_001182533.1.
3D structure databases
ProteinModelPortal	P23748.
SMR	P23748. Positions 244-410.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2713N.
IntAct	P23748. 19 interactions.
MINT	MINT-678695.
STRING	4932.YMR036C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR036C; YMR036C; YMR036C.
GeneID	855052.
KEGG	sce:YMR036C.
Organism-specific databases
CYGD	YMR036c.
SGD	S000004639. MIH1.
Phylogenomic databases
eggNOG	COG5105.
GeneTree	ENSGT00390000018747.
KO	K02555.
OrthoDB	EOG4VQDZR.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32741-MONOMER.
Gene expression databases
Genevestigator	P23748.
GermOnline	YMR036C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR000751. MPI_Phosphatase. IPR001763. Rhodanese-like_dom. [Graphical view]
PANTHER	PTHR10828. PTHR10828. 1 hit.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
PRINTS	PR00716. MPIPHPHTASE.
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978292.

Entry information

Entry name

MPIP_YEAST

Accession

Primary (citable) accession number: P23748
Secondary accession number(s): D6VZL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program