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Protein

M-phase inducer phosphatase

Gene

MIH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Terminates the cell cycle delay. Reverses the CDC28 phosphorylation catalyzed by SWE1.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei320 – 3201By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: SGD

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. G2/M transition of mitotic cell cycle Source: SGD
  3. mitotic nuclear division Source: UniProtKB-KW
  4. peptidyl-tyrosine dephosphorylation Source: GOC
  5. regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32741-MONOMER.
ReactomeiREACT_188487. Cyclin A/B1 associated events during G2/M transition.
REACT_201908. Activation of ATR in response to replication stress.
REACT_220678. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_224255. Cyclin A:Cdk2-associated events at S phase entry.
REACT_245870. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_259128. Polo-like kinase mediated events.
REACT_259368. p53-Independent DNA Damage Response.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase (EC:3.1.3.48)
Alternative name(s):
Mitosis initiation protein MIH1
Mitotic inducer homolog
Gene namesi
Name:MIH1
Ordered Locus Names:YMR036C
ORF Names:YM9532.01C, YM9973.10C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR036c.
SGDiS000004639. MIH1.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554M-phase inducer phosphatasePRO_0000198662Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP23748.

Interactioni

Protein-protein interaction databases

BioGridi35208. 68 interactions.
DIPiDIP-2713N.
IntActiP23748. 15 interactions.
MINTiMINT-678695.
STRINGi4932.YMR036C.

Structurei

3D structure databases

ProteinModelPortaliP23748.
SMRiP23748. Positions 244-410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini261 – 373113RhodanesePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5105.
GeneTreeiENSGT00390000018747.
InParanoidiP23748.
KOiK02555.
OrthoDBiEOG7VMPGR.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23748-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNNIFHGTED ECANEDVLSF QKISLKSPFG KKKNIFRNVQ TFFKSKSKHS
60 70 80 90 100
NVDDDLINKE NLAFDKSPLL TNHRSKEIDG PSPNIKQLGH RDELDENENE
110 120 130 140 150
NDDIVLSMHF ASQTLQSPTR NSSRRSLTNN RDNDLLSRIK YPGSPQRSSS
160 170 180 190 200
FSRSRSLSRK PSMNSSSNSS RRVQRQDGKI PRSSRKSSQK FSNITQNTLN
210 220 230 240 250
FTSASSSPLA PNSVGVKCFE SCLAKTQIPY YYDDRNSNDF FPRISPETLK
260 270 280 290 300
NILQNNMCES FYNSCRIIDC RFEYEYTGGH IINSVNIHSR DELEYEFIHK
310 320 330 340 350
VLHSDTSNNN TLPTLLIIHC EFSSHRGPSL ASHLRNCDRI INQDHYPKLF
360 370 380 390 400
YPDILILDGG YKAVFDNFPE LCYPRQYVGM NSQENLLNCE QEMDKFRRES
410 420 430 440 450
KRFATKNNSF RKLASPSNPN FFYRDSHQSS TTMASSALSF RFEPPPKLSL
460 470 480 490 500
NHRRVSSGSS LNSSESTGDE NFFPILSKSS MSSNSNLSTS HMLLMDGLDT
510 520 530 540 550
PSYFSFEDER GNHQQVSGDE EQDGDFTFVG SDREDLPRPA RRSLFPSLET

EDKK
Length:554
Mass (Da):63,358
Last modified:November 1, 1995 - v2
Checksum:iDBD85D02CF48CA6F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti238 – 2392ND → MT in AAA57223. (PubMed:2649252)Curated
Sequence conflicti241 – 2488FPRISPET → SLEFLQKR in AAA57223. (PubMed:2649252)Curated
Sequence conflicti365 – 3673FDN → LT in AAA57223. (PubMed:2649252)Curated
Sequence conflicti475 – 55480ILSKS…TEDKK → S in AAA57223. (PubMed:2649252)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04846 Genomic DNA. Translation: AAA57223.1.
Z48502 Genomic DNA. Translation: CAA88402.1.
Z49213 Genomic DNA. Translation: CAA89152.1.
BK006946 Genomic DNA. Translation: DAA09935.1.
PIRiA32386.
RefSeqiNP_013750.1. NM_001182533.1.

Genome annotation databases

EnsemblFungiiYMR036C; YMR036C; YMR036C.
GeneIDi855052.
KEGGisce:YMR036C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04846 Genomic DNA. Translation: AAA57223.1.
Z48502 Genomic DNA. Translation: CAA88402.1.
Z49213 Genomic DNA. Translation: CAA89152.1.
BK006946 Genomic DNA. Translation: DAA09935.1.
PIRiA32386.
RefSeqiNP_013750.1. NM_001182533.1.

3D structure databases

ProteinModelPortaliP23748.
SMRiP23748. Positions 244-410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35208. 68 interactions.
DIPiDIP-2713N.
IntActiP23748. 15 interactions.
MINTiMINT-678695.
STRINGi4932.YMR036C.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR036C; YMR036C; YMR036C.
GeneIDi855052.
KEGGisce:YMR036C.

Organism-specific databases

CYGDiYMR036c.
SGDiS000004639. MIH1.

Phylogenomic databases

eggNOGiCOG5105.
GeneTreeiENSGT00390000018747.
InParanoidiP23748.
KOiK02555.
OrthoDBiEOG7VMPGR.

Enzyme and pathway databases

BioCyciYEAST:G3O-32741-MONOMER.
ReactomeiREACT_188487. Cyclin A/B1 associated events during G2/M transition.
REACT_201908. Activation of ATR in response to replication stress.
REACT_220678. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
REACT_224255. Cyclin A:Cdk2-associated events at S phase entry.
REACT_245870. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_259128. Polo-like kinase mediated events.
REACT_259368. p53-Independent DNA Damage Response.

Miscellaneous databases

NextBioi978292.
PROiP23748.

Gene expression databases

GenevestigatoriP23748.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Conservation of mitotic controls in fission and budding yeasts."
    Russell P., Moreno S., Reed S.I.
    Cell 57:295-303(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMPIP_YEAST
AccessioniPrimary (citable) accession number: P23748
Secondary accession number(s): D6VZL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1995
Last modified: February 4, 2015
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.