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Protein

Diacylglycerol kinase alpha

Gene

DGKA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi123 – 1341CuratedAdd BLAST12
Calcium bindingi168 – 1792CuratedAdd BLAST12
Zinc fingeri205 – 253Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri269 – 319Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium ion binding Source: InterPro
  • diacylglycerol kinase activity Source: BHF-UCL
  • kinase activity Source: BHF-UCL
  • phospholipid binding Source: Ensembl

GO - Biological processi

  • diacylglycerol metabolic process Source: BHF-UCL
  • glycerolipid metabolic process Source: BHF-UCL
  • intracellular signal transduction Source: ProtInc
  • lipid phosphorylation Source: BHF-UCL
  • phosphatidic acid biosynthetic process Source: BHF-UCL
  • platelet activation Source: Reactome
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS12171-MONOMER.
BRENDAi2.7.1.107. 2681.
ReactomeiR-HSA-114508. Effects of PIP2 hydrolysis.
SABIO-RKP23743.
SIGNORiP23743.

Chemistry databases

SwissLipidsiSLP:000000555.
SLP:000000739.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase alpha (EC:2.7.1.107)
Short name:
DAG kinase alpha
Alternative name(s):
80 kDa diacylglycerol kinase
Diglyceride kinase alpha
Short name:
DGK-alpha
Gene namesi
Name:DGKA
Synonyms:DAGK, DAGK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:2849. DGKA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Ensembl
  • membrane Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi1606.
OpenTargetsiENSG00000065357.
PharmGKBiPA27310.

Chemistry databases

DrugBankiDB00163. Vitamin E.

Polymorphism and mutation databases

BioMutaiDGKA.
DMDMi281185505.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002184531 – 735Diacylglycerol kinase alphaAdd BLAST735

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei484N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP23743.
MaxQBiP23743.
PaxDbiP23743.
PeptideAtlasiP23743.
PRIDEiP23743.

PTM databases

iPTMnetiP23743.
PhosphoSitePlusiP23743.

Expressioni

Tissue specificityi

Lymphocytes and oligodendroglial cells.

Gene expression databases

BgeeiENSG00000065357.
CleanExiHS_DGKA.
ExpressionAtlasiP23743. baseline and differential.
GenevisibleiP23743. HS.

Organism-specific databases

HPAiHPA041645.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi107976. 4 interactors.
IntActiP23743. 4 interactors.
MINTiMINT-8020268.
STRINGi9606.ENSP00000328405.

Structurei

Secondary structure

1735
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 22Combined sources13
Helixi27 – 35Combined sources9
Helixi38 – 42Combined sources5
Helixi50 – 60Combined sources11
Helixi68 – 77Combined sources10
Helixi96 – 107Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TUZNMR-A1-116[»]
ProteinModelPortaliP23743.
SMRiP23743.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23743.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini110 – 145EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini155 – 190EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini372 – 506DAGKcPROSITE-ProRule annotationAdd BLAST135

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri205 – 253Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri269 – 319Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169. Eukaryota.
ENOG410XQVB. LUCA.
GeneTreeiENSGT00760000119050.
HOVERGENiHBG051345.
InParanoidiP23743.
KOiK00901.
OMAiVGLHCVW.
OrthoDBiEOG091G04A5.
PhylomeDBiP23743.
TreeFamiTF313104.

Family and domain databases

CDDicd00029. C1. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProiIPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
SSF47473. SSF47473. 3 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P23743-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKERGLISP SDFAQLQKYM EYSTKKVSDV LKLFEDGEMA KYVQGDAIGY
60 70 80 90 100
EGFQQFLKIY LEVDNVPRHL SLALFQSFET GHCLNETNVT KDVVCLNDVS
110 120 130 140 150
CYFSLLEGGR PEDKLEFTFK LYDTDRNGIL DSSEVDKIIL QMMRVAEYLD
160 170 180 190 200
WDVSELRPIL QEMMKEIDYD GSGSVSQAEW VRAGATTVPL LVLLGLEMTL
210 220 230 240 250
KDDGQHMWRP KRFPRPVYCN LCESSIGLGK QGLSCNLCKY TVHDQCAMKA
260 270 280 290 300
LPCEVSTYAK SRKDIGVQSH VWVRGGCESG RCDRCQKKIR IYHSLTGLHC
310 320 330 340 350
VWCHLEIHDD CLQAVGHECD CGLLRDHILP PSSIYPSVLA SGPDRKNSKT
360 370 380 390 400
SQKTMDDLNL STSEALRIDP VPNTHPLLVF VNPKSGGKQG QRVLWKFQYI
410 420 430 440 450
LNPRQVFNLL KDGPEIGLRL FKDVPDSRIL VCGGDGTVGW ILETIDKANL
460 470 480 490 500
PVLPPVAVLP LGTGNDLARC LRWGGGYEGQ NLAKILKDLE MSKVVHMDRW
510 520 530 540 550
SVEVIPQQTE EKSDPVPFQI INNYFSIGVD ASIAHRFHIM REKYPEKFNS
560 570 580 590 600
RMKNKLWYFE FATSESIFST CKKLEESLTV EICGKPLDLS NLSLEGIAVL
610 620 630 640 650
NIPSMHGGSN LWGDTRRPHG DIYGINQALG ATAKVITDPD ILKTCVPDLS
660 670 680 690 700
DKRLEVVGLE GAIEMGQIYT KLKNAGRRLA KCSEITFHTT KTLPMQIDGE
710 720 730
PWMQTPCTIK ITHKNQMPML MGPPPRSTNF FGFLS
Length:735
Mass (Da):82,630
Last modified:December 15, 2009 - v3
Checksum:iACAA0AD186EE64A0
GO
Isoform 2 (identifier: P23743-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     117-118: FT → WS
     119-735: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:118
Mass (Da):13,486
Checksum:iDC47336F5D8B584F
GO
Isoform 3 (identifier: P23743-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     237-247: LCKYTVHDQCA → RPITCVGARRL
     248-735: Missing.

Show »
Length:247
Mass (Da):28,094
Checksum:i8B502053A1906FB4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti339L → P in AAC34804 (Ref. 2) Curated1
Sequence conflicti379V → L in AAC34802 (Ref. 2) Curated1
Sequence conflicti385S → W in AAC34802 (Ref. 2) Curated1
Sequence conflicti684E → G in AAC34803 (Ref. 2) Curated1
Sequence conflicti699G → V in CAA44396 (PubMed:2175712).Curated1
Sequence conflicti715N → K in AAC34803 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031563538H → Y.1 PublicationCorresponds to variant rs17852990dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032212117 – 118FT → WS in isoform 2. 1 Publication2
Alternative sequenceiVSP_032213119 – 735Missing in isoform 2. 1 PublicationAdd BLAST617
Alternative sequenceiVSP_047702237 – 247LCKYTVHDQCA → RPITCVGARRL in isoform 3. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_047703248 – 735Missing in isoform 3. 1 PublicationAdd BLAST488

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62535 mRNA. Translation: CAA44396.1.
AF064767 mRNA. Translation: AAC34802.1.
AF064768 mRNA. Translation: AAC34803.1.
AF064769 mRNA. Translation: AAC34804.1.
AF064771 mRNA. Translation: AAC34806.1.
AY930112 mRNA. Translation: AAY20994.1.
AC025162 Genomic DNA. No translation available.
BC023523 mRNA. Translation: AAH23523.1.
BC031870 mRNA. Translation: AAH31870.1.
CCDSiCCDS8896.1. [P23743-1]
PIRiS12969.
RefSeqiNP_001336.2. NM_001345.4. [P23743-1]
NP_958852.1. NM_201444.2. [P23743-1]
NP_958853.1. NM_201445.1. [P23743-1]
NP_963848.1. NM_201554.1. [P23743-1]
XP_011536293.1. XM_011537991.2. [P23743-1]
XP_011536295.1. XM_011537993.2. [P23743-1]
UniGeneiHs.524488.

Genome annotation databases

EnsembliENST00000331886; ENSP00000328405; ENSG00000065357. [P23743-1]
ENST00000394147; ENSP00000377703; ENSG00000065357. [P23743-1]
ENST00000402956; ENSP00000385792; ENSG00000065357. [P23743-3]
ENST00000548549; ENSP00000448565; ENSG00000065357. [P23743-2]
ENST00000551156; ENSP00000450359; ENSG00000065357. [P23743-1]
ENST00000553084; ENSP00000446605; ENSG00000065357. [P23743-2]
GeneIDi1606.
KEGGihsa:1606.
UCSCiuc001sij.4. human. [P23743-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62535 mRNA. Translation: CAA44396.1.
AF064767 mRNA. Translation: AAC34802.1.
AF064768 mRNA. Translation: AAC34803.1.
AF064769 mRNA. Translation: AAC34804.1.
AF064771 mRNA. Translation: AAC34806.1.
AY930112 mRNA. Translation: AAY20994.1.
AC025162 Genomic DNA. No translation available.
BC023523 mRNA. Translation: AAH23523.1.
BC031870 mRNA. Translation: AAH31870.1.
CCDSiCCDS8896.1. [P23743-1]
PIRiS12969.
RefSeqiNP_001336.2. NM_001345.4. [P23743-1]
NP_958852.1. NM_201444.2. [P23743-1]
NP_958853.1. NM_201445.1. [P23743-1]
NP_963848.1. NM_201554.1. [P23743-1]
XP_011536293.1. XM_011537991.2. [P23743-1]
XP_011536295.1. XM_011537993.2. [P23743-1]
UniGeneiHs.524488.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TUZNMR-A1-116[»]
ProteinModelPortaliP23743.
SMRiP23743.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107976. 4 interactors.
IntActiP23743. 4 interactors.
MINTiMINT-8020268.
STRINGi9606.ENSP00000328405.

Chemistry databases

DrugBankiDB00163. Vitamin E.
SwissLipidsiSLP:000000555.
SLP:000000739.

PTM databases

iPTMnetiP23743.
PhosphoSitePlusiP23743.

Polymorphism and mutation databases

BioMutaiDGKA.
DMDMi281185505.

Proteomic databases

EPDiP23743.
MaxQBiP23743.
PaxDbiP23743.
PeptideAtlasiP23743.
PRIDEiP23743.

Protocols and materials databases

DNASUi1606.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000331886; ENSP00000328405; ENSG00000065357. [P23743-1]
ENST00000394147; ENSP00000377703; ENSG00000065357. [P23743-1]
ENST00000402956; ENSP00000385792; ENSG00000065357. [P23743-3]
ENST00000548549; ENSP00000448565; ENSG00000065357. [P23743-2]
ENST00000551156; ENSP00000450359; ENSG00000065357. [P23743-1]
ENST00000553084; ENSP00000446605; ENSG00000065357. [P23743-2]
GeneIDi1606.
KEGGihsa:1606.
UCSCiuc001sij.4. human. [P23743-1]

Organism-specific databases

CTDi1606.
DisGeNETi1606.
GeneCardsiDGKA.
HGNCiHGNC:2849. DGKA.
HPAiHPA041645.
MIMi125855. gene.
neXtProtiNX_P23743.
OpenTargetsiENSG00000065357.
PharmGKBiPA27310.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1169. Eukaryota.
ENOG410XQVB. LUCA.
GeneTreeiENSGT00760000119050.
HOVERGENiHBG051345.
InParanoidiP23743.
KOiK00901.
OMAiVGLHCVW.
OrthoDBiEOG091G04A5.
PhylomeDBiP23743.
TreeFamiTF313104.

Enzyme and pathway databases

BioCyciZFISH:HS12171-MONOMER.
BRENDAi2.7.1.107. 2681.
ReactomeiR-HSA-114508. Effects of PIP2 hydrolysis.
SABIO-RKP23743.
SIGNORiP23743.

Miscellaneous databases

ChiTaRSiDGKA. human.
EvolutionaryTraceiP23743.
GeneWikiiDGKA.
GenomeRNAii1606.
PROiP23743.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000065357.
CleanExiHS_DGKA.
ExpressionAtlasiP23743. baseline and differential.
GenevisibleiP23743. HS.

Family and domain databases

CDDicd00029. C1. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProiIPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
SSF47473. SSF47473. 3 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDGKA_HUMAN
AccessioniPrimary (citable) accession number: P23743
Secondary accession number(s): O75481
, O75482, O75483, O95217, Q3ZE25, Q8IZ56, Q8N5Q2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: December 15, 2009
Last modified: November 30, 2016
This is version 178 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.