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P23737

- MTS9_STAAU

UniProt

P23737 - MTS9_STAAU

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Protein

Modification methylase Sau96I

Gene

sau96IM

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the Sau96I endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei174 – 1741PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Protein family/group databases

REBASEi3496. M.Sau96I.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase Sau96I (EC:2.1.1.37)
Short name:
M.Sau96I
Alternative name(s):
Cytosine-specific methyltransferase Sau96I
Gene namesi
Name:sau96IM
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 430430Modification methylase Sau96IPRO_0000087908Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP23737.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 6355HTH cro/C1-typePROSITE-ProRule annotationAdd
BLAST
Domaini99 – 429331SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
PF01381. HTH_3. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SMARTiSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS50943. HTH_CROC1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23737-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLNKGSIIE KMKNQNIKTQ TELAEKIDIS KSQLSFMFSD EYEPLKKNVI
60 70 80 90 100
KLADVLKVSP NDIILDEEDQ MPINSDFNRY DYKLDEFIDV SNVRKNKDYN
110 120 130 140 150
VFETFAGAGG LALGLESAGL STYGAVEIDK NAAETLRINR PKWKVIENDI
160 170 180 190 200
EFIADNLDEF IDEEIDILSG GYPCQTFSYA GKRNGFADTR GTLFYPYSKI
210 220 230 240 250
LSKLKPKAFI AENVRGLVNH DDGKTLEVML KVFIKEGYEV YWNILNSWNY
260 270 280 290 300
DVAQKRERIV IIGIREDLVK EQKYPFRFPL AQVYKPVLKD VLKDVPKSKV
310 320 330 340 350
TAYSDKKREV MKLVPPGGCW VDLPEQIAKD YMGKSWYSGG GKRGMARRIS
360 370 380 390 400
WDEPCLTLTT SPSQKQTERC HPDETRPFSI REYARIQSFP DEWEFSGGVG
410 420 430
AQYRQIGNAV PVNLAKYIGK SLVHYLNQFN
Length:430
Mass (Da):49,284
Last modified:November 1, 1991 - v1
Checksum:i884FBD96F4890651
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53096 Genomic DNA. Translation: CAA37260.1.
PIRiS12705.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53096 Genomic DNA. Translation: CAA37260.1 .
PIRi S12705.

3D structure databases

ProteinModelPortali P23737.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3496. M.Sau96I.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.260.40. 1 hit.
3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
PF01381. HTH_3. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SMARTi SM00530. HTH_XRE. 1 hit.
[Graphical view ]
SUPFAMi SSF47413. SSF47413. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS50943. HTH_CROC1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes."
    Szilak L., Venetianer P., Kiss A.
    Nucleic Acids Res. 18:4659-4664(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: PS96.

Entry informationi

Entry nameiMTS9_STAAU
AccessioniPrimary (citable) accession number: P23737
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 1, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3