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P23737

- MTS9_STAAU

UniProt

P23737 - MTS9_STAAU

Protein

Modification methylase Sau96I

Gene

sau96IM

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the Sau96I endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei174 – 1741PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. sequence-specific DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3496. M.Sau96I.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase Sau96I (EC:2.1.1.37)
    Short name:
    M.Sau96I
    Alternative name(s):
    Cytosine-specific methyltransferase Sau96I
    Gene namesi
    Name:sau96IM
    OrganismiStaphylococcus aureus
    Taxonomic identifieri1280 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 430430Modification methylase Sau96IPRO_0000087908Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP23737.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 6355HTH cro/C1-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini99 – 429331SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 HTH cro/C1-type DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.10.260.40. 1 hit.
    3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR001387. Cro/C1-type_HTH.
    IPR010982. Lambda_DNA-bd_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    PF01381. HTH_3. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SMARTiSM00530. HTH_XRE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47413. SSF47413. 1 hit.
    SSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS50943. HTH_CROC1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23737-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLNKGSIIE KMKNQNIKTQ TELAEKIDIS KSQLSFMFSD EYEPLKKNVI    50
    KLADVLKVSP NDIILDEEDQ MPINSDFNRY DYKLDEFIDV SNVRKNKDYN 100
    VFETFAGAGG LALGLESAGL STYGAVEIDK NAAETLRINR PKWKVIENDI 150
    EFIADNLDEF IDEEIDILSG GYPCQTFSYA GKRNGFADTR GTLFYPYSKI 200
    LSKLKPKAFI AENVRGLVNH DDGKTLEVML KVFIKEGYEV YWNILNSWNY 250
    DVAQKRERIV IIGIREDLVK EQKYPFRFPL AQVYKPVLKD VLKDVPKSKV 300
    TAYSDKKREV MKLVPPGGCW VDLPEQIAKD YMGKSWYSGG GKRGMARRIS 350
    WDEPCLTLTT SPSQKQTERC HPDETRPFSI REYARIQSFP DEWEFSGGVG 400
    AQYRQIGNAV PVNLAKYIGK SLVHYLNQFN 430
    Length:430
    Mass (Da):49,284
    Last modified:November 1, 1991 - v1
    Checksum:i884FBD96F4890651
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53096 Genomic DNA. Translation: CAA37260.1.
    PIRiS12705.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53096 Genomic DNA. Translation: CAA37260.1 .
    PIRi S12705.

    3D structure databases

    ProteinModelPortali P23737.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3496. M.Sau96I.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.260.40. 1 hit.
    3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR001387. Cro/C1-type_HTH.
    IPR010982. Lambda_DNA-bd_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    PF01381. HTH_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SMARTi SM00530. HTH_XRE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47413. SSF47413. 1 hit.
    SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS50943. HTH_CROC1. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes."
      Szilak L., Venetianer P., Kiss A.
      Nucleic Acids Res. 18:4659-4664(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: PS96.

    Entry informationi

    Entry nameiMTS9_STAAU
    AccessioniPrimary (citable) accession number: P23737
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3