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P23737 (MTS9_STAAU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase Sau96I

Short name=M.Sau96I
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase Sau96I
Gene names
Name:sau96IM
OrganismStaphylococcus aureus
Taxonomic identifier1280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length430 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the Sau96I endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 HTH cro/C1-type DNA-binding domain.

Contains 1 SAM-dependent MTase C5-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 430430Modification methylase Sau96I
PRO_0000087908

Regions

Domain9 – 6355HTH cro/C1-type
Domain99 – 429331SAM-dependent MTase C5-type

Sites

Active site1741 By similarity

Sequences

Sequence LengthMass (Da)Tools
P23737 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 884FBD96F4890651

FASTA43049,284
        10         20         30         40         50         60 
MRLNKGSIIE KMKNQNIKTQ TELAEKIDIS KSQLSFMFSD EYEPLKKNVI KLADVLKVSP 

        70         80         90        100        110        120 
NDIILDEEDQ MPINSDFNRY DYKLDEFIDV SNVRKNKDYN VFETFAGAGG LALGLESAGL 

       130        140        150        160        170        180 
STYGAVEIDK NAAETLRINR PKWKVIENDI EFIADNLDEF IDEEIDILSG GYPCQTFSYA 

       190        200        210        220        230        240 
GKRNGFADTR GTLFYPYSKI LSKLKPKAFI AENVRGLVNH DDGKTLEVML KVFIKEGYEV 

       250        260        270        280        290        300 
YWNILNSWNY DVAQKRERIV IIGIREDLVK EQKYPFRFPL AQVYKPVLKD VLKDVPKSKV 

       310        320        330        340        350        360 
TAYSDKKREV MKLVPPGGCW VDLPEQIAKD YMGKSWYSGG GKRGMARRIS WDEPCLTLTT 

       370        380        390        400        410        420 
SPSQKQTERC HPDETRPFSI REYARIQSFP DEWEFSGGVG AQYRQIGNAV PVNLAKYIGK 

       430 
SLVHYLNQFN 

« Hide

References

[1]"Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes."
Szilak L., Venetianer P., Kiss A.
Nucleic Acids Res. 18:4659-4664(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: PS96.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53096 Genomic DNA. Translation: CAA37260.1.
PIRS12705.

3D structure databases

ProteinModelPortalP23737.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3496. M.Sau96I.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.260.40. 1 hit.
3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR001387. Cro/C1-type_HTH.
IPR010982. Lambda_DNA-bd_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
PF01381. HTH_3. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SMARTSM00530. HTH_XRE. 1 hit.
[Graphical view]
SUPFAMSSF47413. SSF47413. 1 hit.
SSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS50943. HTH_CROC1. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTS9_STAAU
AccessionPrimary (citable) accession number: P23737
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 11, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries