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P23736 (T2S9_STAAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme Sau96I

Short name=R.Sau96I
EC=3.1.21.4
Alternative name(s):
Endonuclease Sau96I
Type II restriction enzyme Sau96I
Gene names
Name:sau96IR
OrganismStaphylococcus aureus
Taxonomic identifier1280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGNCC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 261261Type-2 restriction enzyme Sau96I
PRO_0000077359

Sequences

Sequence LengthMass (Da)Tools
P23736 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 75338416FCD54677

FASTA26130,486
        10         20         30         40         50         60 
MTNKYLSFIT DEDLFECIEF LYTEYEKALE GIDFDKFFKN RIDTFKMTFD MGINNLSEQD 

        70         80         90        100        110        120 
WLAAELQRQV EKTITNHVGT FHEKLIGKIE GYTNYPVGYD YDVAKDDNTL FAEIKNKHNT 

       130        140        150        160        170        180 
LTGTHTKSLF QKICGYAEKY PDAICYYVRI IDTKSRNDIW EFRSGSIDEN TREKPRFSHP 

       190        200        210        220        230        240 
RVRIASGDQF YKIVTGEEDA FKQLAYNIPI ALDDWIETKR AKKGSSLGLF AELYQQAKAN 

       250        260 
NRTLSEEIIS INYPKSNYIS F 

« Hide

References

[1]"Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes."
Szilak L., Venetianer P., Kiss A.
Nucleic Acids Res. 18:4659-4664(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: PS96.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53096 Genomic DNA. Translation: CAA37259.1.
PIRS12706.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1607. Sau96I.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019057. Restrct_endonuc_II_Eco47II.
[Graphical view]
PfamPF09553. RE_Eco47II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2S9_STAAU
AccessionPrimary (citable) accession number: P23736
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 16, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries