Reviewed, UniProtKB/Swiss-Prot P23736 (T2S9_STAAU)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme Sau96I
      Short name=R.Sau96I
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme Sau96I
    Endonuclease Sau96I

Gene names

Name:

sau96IR

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GGNCC and cleaves after G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Type-2 restriction enzyme Sau96I		PRO_0000077359

Sequences

	Sequence		Length	Mass (Da)
	P23736-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 75338416FCD54677	FASTA	261	30,486
	10 20 30 40 50 60 MTNKYLSFIT DEDLFECIEF LYTEYEKALE GIDFDKFFKN RIDTFKMTFD MGINNLSEQD 70 80 90 100 110 120 WLAAELQRQV EKTITNHVGT FHEKLIGKIE GYTNYPVGYD YDVAKDDNTL FAEIKNKHNT 130 140 150 160 170 180 LTGTHTKSLF QKICGYAEKY PDAICYYVRI IDTKSRNDIW EFRSGSIDEN TREKPRFSHP 190 200 210 220 230 240 RVRIASGDQF YKIVTGEEDA FKQLAYNIPI ALDDWIETKR AKKGSSLGLF AELYQQAKAN 250 260 NRTLSEEIIS INYPKSNYIS F

References

[1]	"Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes." Szilak L., Venetianer P., Kiss A. Nucleic Acids Res. 18:4659-4664(1990) [PubMed: 2204026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PS96.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53096 Genomic DNA. Translation: CAA37259.1.
PIR	S12706.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1607. Sau96I.
Enzyme and pathway databases
BRENDA	3.1.21.4. 95.
Family and domain databases
InterPro	IPR019057. Restrict_endonuc_II_Eco47II. [Graphical view]
Pfam	PF09553. RE_Eco47II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2S9_STAAU

Accession

Primary (citable) accession number: P23736

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)