P23734 (PP12_TRYBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Serine/threonine-protein phosphatase PP1(5.9) EC=3.1.3.16
Organism	Trypanosoma brucei brucei
Taxonomic identifier	5702 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma › Trypanozoon › Trypanosoma brucei

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Miscellaneous	Trypanosoma brucei contains two PP1 genes which are highly similar.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Serine/threonine-protein phosphatase PP1(5.9)		PRO_0000058815
Sites
	Active site	163	1	Proton donor By similarity
	Metal binding	102	1	Iron By similarity
	Metal binding	104	1	Iron By similarity
	Metal binding	130	1	Iron By similarity
	Metal binding	130	1	Manganese By similarity
	Metal binding	162	1	Manganese By similarity
	Metal binding	211	1	Manganese By similarity
	Metal binding	287	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23734 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 1CC8700523F0FB36	FASTA	346	39,295
	10 20 30 40 50 60 MNCREIIRKL LLNPAHNNAA TRTAQGDNGD SNQRAYTRIS RLAAFQSAQT QESTPKTNGT 70 80 90 100 110 120 GRATTEGLTE AEVRWLVMES RALFMSQPML VEIAAPVRIC GDVHGQYTDL LRLFDLGGFP 130 140 150 160 170 180 PDANYIFLGD YVDRGDQSLE TICLLLAYKL SFPETFFLLR GNHECSSINR IYGFFDECKR 190 200 210 220 230 240 RYSVRLWKQF TDTFNCMPVA GLVEGRILCM HGGLSPELTD LDQIRRILRP TDVPDSGLIC 250 260 270 280 290 300 DLLWSDPSTN MESNWSENDR GVSWTFSESV VKSFNKKFDL DLICRAHQVV DAGYEFFAAR 310 320 330 340 QLVTVFSAPN YCDEFDNAGA FMCVDENLMC SFVQIEPTRT LLRYFF

References

[1]	"The Trypanosoma brucei protein phosphatase gene: polycistronic transcription with the RNA polymerase II largest subunit gene." Evers R., Cornelissen A.W.C.A. Nucleic Acids Res. 18:5089-5095(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52746 Genomic DNA. Translation: CAA36960.1.
PIR	S12599.
3D structure databases
ProteinModelPortal	P23734.
SMR	P23734. Positions 68-344.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP12_TRYBB

Accession

Primary (citable) accession number: P23734

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)