Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphatidylinositol 3-kinase regulatory subunit beta

Gene

PIK3R2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy. Promotes nuclear translocation of XBP1 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Stress response, Transport

Enzyme and pathway databases

ReactomeiR-BTA-109704 PI3K Cascade
R-BTA-114604 GPVI-mediated activation cascade
R-BTA-1257604 PIP3 activates AKT signaling
R-BTA-1266695 Interleukin-7 signaling
R-BTA-1433557 Signaling by SCF-KIT
R-BTA-1660499 Synthesis of PIPs at the plasma membrane
R-BTA-186763 Downstream signal transduction
R-BTA-194840 Rho GTPase cycle
R-BTA-198203 PI3K/AKT activation
R-BTA-202424 Downstream TCR signaling
R-BTA-2029485 Role of phospholipids in phagocytosis
R-BTA-210993 Tie2 Signaling
R-BTA-2424491 DAP12 signaling
R-BTA-2730905 Role of LAT2/NTAL/LAB on calcium mobilization
R-BTA-388841 Costimulation by the CD28 family
R-BTA-389357 CD28 dependent PI3K/Akt signaling
R-BTA-392451 G beta:gamma signalling through PI3Kgamma
R-BTA-416476 G alpha (q) signalling events
R-BTA-416482 G alpha (12/13) signalling events
R-BTA-4420097 VEGFA-VEGFR2 Pathway
R-BTA-512988 Interleukin-3, 5 and GM-CSF signaling
R-BTA-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-BTA-8853659 RET signaling
R-BTA-912526 Interleukin receptor SHC signaling
R-BTA-912631 Regulation of signaling by CBL

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase regulatory subunit beta
Short name:
PI3-kinase regulatory subunit beta
Short name:
PI3K regulatory subunit beta
Short name:
PtdIns-3-kinase regulatory subunit beta
Alternative name(s):
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
Short name:
PI3-kinase subunit p85-beta
Short name:
PtdIns-3-kinase regulatory subunit p85-beta
Gene namesi
Name:PIK3R2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 7

Organism-specific databases

VGNCiVGNC:52862 PIK3R2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000807621 – 724Phosphatidylinositol 3-kinase regulatory subunit betaAdd BLAST724

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei460PhosphotyrosineBy similarity1
Modified residuei601PhosphotyrosineBy similarity1
Modified residuei651PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated in response to signaling from activated receptor-type protein kinases. Dephosphorylated by PTPRJ. Dephosphorylated at Tyr-651 by PTPN13. Phosphorylation of Tyr-651 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination.By similarity
Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP23726
PRIDEiP23726

Expressioni

Gene expression databases

BgeeiENSBTAG00000002350

Interactioni

Subunit structurei

Heterodimer of a regulatory subunit PIK3R2 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Interacts with FLT1 (tyrosine-phosphorylated) and FLT4 (tyrosine-phosphorylated). Interacts with NYAP1, NYAP2 and MYO16. Interacts with FBXL2; PIK3R2 is a substrate of the SCF(FBXL2) complex. Interacts with PTPN13; dephosphorylates PIK3R2. Interacts with XBP1; the interaction is direct and induces translocation of XBP1 into the nucleus in a ER stress- and/or insulin-dependent but PI3K-independent manner. Interacts with PIK3R1; the interaction is dissociated in an insulin-dependent manner (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PIK3CAP328713EBI-1555978,EBI-1373130

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP23726
DIPiDIP-39878N
IntActiP23726, 3 interactors
MINTiP23726
STRINGi9913.ENSBTAP00000003033

Structurei

Secondary structure

1724
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi432 – 494Combined sources63
Beta strandi495 – 497Combined sources3
Helixi500 – 502Combined sources3
Beta strandi503 – 507Combined sources5
Beta strandi511 – 513Combined sources3
Turni514 – 518Combined sources5
Helixi519 – 579Combined sources61
Helixi584 – 591Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3L4QX-ray2.30C/D424-593[»]
ProteinModelPortaliP23726
SMRiP23726
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23726

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 80SH3PROSITE-ProRule annotationAdd BLAST77
Domaini109 – 291Rho-GAPPROSITE-ProRule annotationAdd BLAST183
Domaini326 – 421SH2 1PROSITE-ProRule annotationAdd BLAST96
Domaini618 – 712SH2 2PROSITE-ProRule annotationAdd BLAST95

Domaini

The SH2 2 domain is required for interaction with FBXL2 and PTPN13.By similarity

Sequence similaritiesi

Belongs to the PI3K p85 subunit family.Curated

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG4637 Eukaryota
ENOG410XP6R LUCA
GeneTreeiENSGT00390000010431
HOGENOMiHOG000008438
HOVERGENiHBG082100
InParanoidiP23726
KOiK02649
OMAiHCVIYKT
OrthoDBiEOG091G0C3Z
TreeFamiTF102033

Family and domain databases

CDDicd09930 SH2_cSH2_p85_like, 1 hit
cd09942 SH2_nSH2_p85_like, 1 hit
cd11909 SH3_PI3K_p85beta, 1 hit
Gene3Di1.10.555.10, 1 hit
3.30.505.10, 2 hits
InterProiView protein in InterPro
IPR032498 PI3K_P85_iSH2
IPR035586 PI3K_p85beta_SH3
IPR035020 PI3kinase_P85_cSH2
IPR035022 PI3kinase_P85_nSH2
IPR001720 PI3kinase_P85_p55
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR10155 PTHR10155, 1 hit
PfamiView protein in Pfam
PF16454 PI3K_P85_iSH2, 1 hit
PF00620 RhoGAP, 1 hit
PF00017 SH2, 2 hits
PRINTSiPR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00324 RhoGAP, 1 hit
SM00252 SH2, 2 hits
SM00326 SH3, 1 hit
SUPFAMiSSF48350 SSF48350, 1 hit
SSF50044 SSF50044, 1 hit
SSF55550 SSF55550, 2 hits
PROSITEiView protein in PROSITE
PS50238 RHOGAP, 1 hit
PS50001 SH2, 2 hits
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

P23726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGPEGFQYR ALYPFRRERP EDLELLPGDV LVVSRAALQA LGVAEGNERC
60 70 80 90 100
PQSVGWMPGL NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP
110 120 130 140 150
RDGPPEPGLT LPDLPEQFSP PDVAPPILVK LVEAIERTGL DSYRPEPPAV
160 170 180 190 200
RTDWSLSDVE QWDAAALSDG VKGFLLALPA PLVTPEAAAE AHRALREAAG
210 220 230 240 250
PVGPALEPPT LPLHHALTLR FLLQHLGRVA GRAPAPGPAV RALGATFGPL
260 270 280 290 300
LLRAPPPPSP PPGGAPDGTE PTPDFPALLV EKLLQEHLEE QEVAPPALPP
310 320 330 340 350
KPPKTKPAPT GLANGGSPPS LQDAEWYWGD ISREEVNEKL RDTPDGTFLV
360 370 380 390 400
RDASSKIQGE YTLTLRKGGN NKLIKVFHRD GHYGFSEPLT FCSVVDLITH
410 420 430 440 450
YRHESLAQYN AKLDTRLLYP VSKYQQDQIV KEDSVEAVGA QLKVYHQQYQ
460 470 480 490 500
DKSREYDQLY EEYTRTSQEL QMKRTAIEAF NETIKIFEEQ GQTQEKCSKE
510 520 530 540 550
YLERFRREGN EKEMQRILLN SERLKSRIAE IHESRTKLEQ ELRAQASDNR
560 570 580 590 600
EIDKRMNSLK PDLMQLRKIR DQYLVWLTQK GARQKKINEW LGIKNETEDQ
610 620 630 640 650
YSLMEDEDDL PHHEERTWYV GKINRTQAEE MLSGKRDGTF LIRESSQRGC
660 670 680 690 700
YACSVVVDGD TKHCVIYRTA TGFGFAEPYN LYGSLKELVL HYQHASLVQH
710 720
NDALTVTLAH PVRAPGPGPP PAAR
Length:724
Mass (Da):81,060
Last modified:November 1, 1991 - v1
Checksum:i9D2BA8B6DB087098
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61746 mRNA Translation: AAA79510.1
BC118113 mRNA Translation: AAI18114.1
PIRiB38749
RefSeqiNP_777001.1, NM_174576.2
UniGeneiBt.515

Genome annotation databases

EnsembliENSBTAT00000003033; ENSBTAP00000003033; ENSBTAG00000002350
GeneIDi282308
KEGGibta:282308

Similar proteinsi

Entry informationi

Entry nameiP85B_BOVIN
AccessioniPrimary (citable) accession number: P23726
Secondary accession number(s): Q17QY7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health