Reviewed, UniProtKB/Swiss-Prot P23726 (P85B_BOVIN)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 3-kinase regulatory subunit beta
Alternative name(s):
    PI3-kinase p85 subunit beta
    PtdIns-3-kinase p85-beta

Gene names

Name:

PIK3R2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
Subunit structure	Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits.
Sequence similarities	Belongs to the PI3K p85 subunit family. Contains 1 Rho-GAP domain. Contains 2 SH2 domains. Contains 1 SH3 domain.

Ontologies

Keywords
Domain	Repeat SH2 domain SH3 domain
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	negative regulation of anti-apoptosis Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	phosphoinositide 3-kinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	phosphoinositide 3-kinase regulator activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PIK3CA	P32871	2	EBI-1555978,EBI-1373130

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Phosphatidylinositol 3-kinase regulatory subunit beta		PRO_0000080762
Regions
	Domain	4 – 80	77	SH3
	Domain	109 – 291	183	Rho-GAP
	Domain	326 – 421	96	SH2 1
	Domain	618 – 712	95	SH2 2
Amino acid modifications
	Modified residue	361	1	Phosphotyrosine By similarity
	Modified residue	460	1	Phosphotyrosine By similarity
	Modified residue	463	1	Phosphotyrosine By similarity
	Modified residue	601	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23726-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 9D2BA8B6DB087098	FASTA	724	81,060
	10 20 30 40 50 60 MAGPEGFQYR ALYPFRRERP EDLELLPGDV LVVSRAALQA LGVAEGNERC PQSVGWMPGL 70 80 90 100 110 120 NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP RDGPPEPGLT LPDLPEQFSP 130 140 150 160 170 180 PDVAPPILVK LVEAIERTGL DSYRPEPPAV RTDWSLSDVE QWDAAALSDG VKGFLLALPA 190 200 210 220 230 240 PLVTPEAAAE AHRALREAAG PVGPALEPPT LPLHHALTLR FLLQHLGRVA GRAPAPGPAV 250 260 270 280 290 300 RALGATFGPL LLRAPPPPSP PPGGAPDGTE PTPDFPALLV EKLLQEHLEE QEVAPPALPP 310 320 330 340 350 360 KPPKTKPAPT GLANGGSPPS LQDAEWYWGD ISREEVNEKL RDTPDGTFLV RDASSKIQGE 370 380 390 400 410 420 YTLTLRKGGN NKLIKVFHRD GHYGFSEPLT FCSVVDLITH YRHESLAQYN AKLDTRLLYP 430 440 450 460 470 480 VSKYQQDQIV KEDSVEAVGA QLKVYHQQYQ DKSREYDQLY EEYTRTSQEL QMKRTAIEAF 490 500 510 520 530 540 NETIKIFEEQ GQTQEKCSKE YLERFRREGN EKEMQRILLN SERLKSRIAE IHESRTKLEQ 550 560 570 580 590 600 ELRAQASDNR EIDKRMNSLK PDLMQLRKIR DQYLVWLTQK GARQKKINEW LGIKNETEDQ 610 620 630 640 650 660 YSLMEDEDDL PHHEERTWYV GKINRTQAEE MLSGKRDGTF LIRESSQRGC YACSVVVDGD 670 680 690 700 710 720 TKHCVIYRTA TGFGFAEPYN LYGSLKELVL HYQHASLVQH NDALTVTLAH PVRAPGPGPP PAAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase." Otsu M., Hiles I.D., Goot I., Fry M.J., Ruiz-Larrea F., Panayotou G., Thompson A., Dhand R., Hsuan J., Totty N., Smith A.D., Morgan S.J., Courtneidge S.A., Parker P.J., Waterfield M.D. Cell 65:91-104(1991) [PubMed: 1707345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Uterus.
[3]	"Interaction of the p85 subunit of PI 3-kinase and its N-terminal SH2 domain with a PDGF receptor phosphorylation site: structural features and analysis of conformational changes." Panayotou G., Bax B., Gout I., Federwisch M., Wroblowski B., Dhand R., Fry M.J., Blundell T.L., Wollmer A., Waterfield M.D. EMBO J. 11:4261-4272(1992) [PubMed: 1330535] [Abstract] Cited for: CIRCULAR DICHROISM ANALYSIS, FLUORESCENCE SPECTROSCOPY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M61746 mRNA. Translation: AAA79510.1. BC118113 mRNA. Translation: AAI18114.1.
IPI	IPI00710112.
PIR	B38749.
RefSeq	NP_777001.1.
UniGene	Bt.515
3D structure databases
SMR	P23726. Positions 320-432, 610-714.
ModBase	Search...
Protein-protein interaction databases
IntAct	P23726. 2 interactions.
STRING	P23726.
Proteomic databases
PRIDE	P23726.
Genome annotation databases
Ensembl	ENSBTAT00000003033; ENSBTAP00000003033; ENSBTAG00000002350; Bos taurus. [Genome view]
GeneID	282308.
KEGG	bta:282308.
Organism-specific databases
CTD	282308.
Phylogenomic databases
HOVERGEN	P23726.
OMA	DEDDLPH
OrthoDB	EOG9HDWCT
Family and domain databases
InterPro	IPR001720. PI3kinase_P85. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP. IPR000980. SH2. IPR001452. SH3_domain. [Graphical view]
Gene3D	G3DSA:1.10.555.10. RhoGAP. 1 hit. G3DSA:3.30.505.10. SH2. 2 hits.
PANTHER	PTHR10155. PI3kinase_P85. 1 hit.
Pfam	PF00620. RhoGAP. 1 hit. PF00017. SH2. 2 hits. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00678. PI3KINASEP85. PR00401. SH2DOMAIN.
SMART	SM00324. RhoGAP. 1 hit. SM00252. SH2. 2 hits. SM00326. SH3. 1 hit. [Graphical view]
PROSITE	PS50238. RHOGAP. 1 hit. PS50001. SH2. 2 hits. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P85B_BOVIN

Accession

Primary (citable) accession number: P23726
Secondary accession number(s): Q17QY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	November 24, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)