Reviewed, UniProtKB/Swiss-Prot P23725 (PUTA_KLEPN)

Names and origin

Protein names

Recommended name:
    Bifunctional protein putA
Including the following 2 domains:
    1- Recommended name:
            Proline dehydrogenase
              EC=1.5.99.8
        Alternative name(s):
            Proline oxidase
    2- Recommended name:
            Delta-1-pyrroline-5-carboxylate dehydrogenase
                Short name=P5C dehydrogenase
              EC=1.5.1.12

Gene names

Name:

putA

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	26 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon.
Catalytic activity	L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor. (S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H.
Cofactor	FAD.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2. Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
Induction	By proline, autorepression and catabolite repression, and is potentially nitrogen controlled.

Ontologies

Keywords
Biological process	Proline metabolism Transcription Transcription regulation
Ligand	DNA-binding FAD Flavoprotein NAD
Molecular function	Oxidoreductase Repressor
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline metabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: UniProtKB-KW proline dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›26	›26	Bifunctional protein putA		PRO_0000056526
Experimental info
	Non-terminal residue	26	1

Sequences

	Sequence		Length	Mass (Da)
	P23725-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: BB332D0DE504CE19	FASTA	26	2,824
	10 20 MGTTTMGVKL DDATRERIKS AASRID

References

[1]	"Regulation of proline utilization in enteric bacteria: cloning and characterization of the Klebsiella put control region." Chen L.M., Maloy S. J. Bacteriol. 173:783-790(1991) [PubMed: 1987164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63160 Genomic DNA. Translation: AAA25139.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.5.1.12. 758. 1.5.99.8. 758.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. Partial match. PS00687. ALDEHYDE_DEHYDR_GLU. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUTA_KLEPN

Accession

Primary (citable) accession number: P23725

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)