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Protein

NADH-ubiquinone oxidoreductase 30.4 kDa subunit, mitochondrial

Gene

nuo-31

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

NAD, Ubiquinone

Protein family/group databases

TCDBi3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 30.4 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
CI-31kD
Complex I-30kD
Gene namesi
Name:nuo-31
ORF Names:NCU04074
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 5, Linkage Group VI

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-SubCell
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1717MitochondrionSequence AnalysisAdd
BLAST
Chaini18 – 283266NADH-ubiquinone oxidoreductase 30.4 kDa subunit, mitochondrialPRO_0000020000Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of about 40 different subunits. This is a component of the iron-sulfur protein fraction.

Protein-protein interaction databases

STRINGi5141.NCU04074.1.

Structurei

3D structure databases

ProteinModelPortaliP23710.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 30 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0852.
HOGENOMiHOG000009797.
InParanoidiP23710.
KOiK03936.
OMAiLRDHSQC.
OrthoDBiEOG7ZGXDG.

Family and domain databases

HAMAPiMF_01357. NDH1_NuoC.
InterProiIPR010218. NADH_DH_suC.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01961. NuoC_fam. 1 hit.
PROSITEiPS00542. COMPLEX1_30K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23710-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASKLCRSRA LASALRSAKP SPAIRCLATT SRNLINMPEG PNPRQFPREP
60 70 80 90 100
LPGALNAAVV NPADKYQSKA DNLHKYGSWL MGCLPKYIQQ FSVWKDELTI
110 120 130 140 150
YISPAGVIPV FSFLKYNTAA EYTQVSDITA VDFPTKDQRF EVVYNLLSVR
160 170 180 190 200
HNSRIRVKTY ADEVSPVPSI TPLYDGANWY EREVYDLFGV FFTGHPDLRR
210 220 230 240 250
IMTDYGFDGH PLRKDFPMTG YTEIRYDEEK KRIVTEPLEM TQAFRNFEGG
260 270 280
SSAWEQVGAG IDRKPESFKL PTPKPETKPE EKK
Length:283
Mass (Da):32,185
Last modified:June 7, 2005 - v2
Checksum:i3A2DCD3489F32C6F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401G → R(PubMed:2145832)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM002241 Genomic DNA. Translation: EAA28453.1.
PIRiA35935.
RefSeqiXP_957689.1. XM_952596.2.

Genome annotation databases

EnsemblFungiiEFNCRT00000003652; EFNCRP00000003652; EFNCRG00000003648.
GeneIDi3873779.
KEGGincr:NCU04074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM002241 Genomic DNA. Translation: EAA28453.1.
PIRiA35935.
RefSeqiXP_957689.1. XM_952596.2.

3D structure databases

ProteinModelPortaliP23710.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5141.NCU04074.1.

Protein family/group databases

TCDBi3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000003652; EFNCRP00000003652; EFNCRG00000003648.
GeneIDi3873779.
KEGGincr:NCU04074.

Phylogenomic databases

eggNOGiCOG0852.
HOGENOMiHOG000009797.
InParanoidiP23710.
KOiK03936.
OMAiLRDHSQC.
OrthoDBiEOG7ZGXDG.

Family and domain databases

HAMAPiMF_01357. NDH1_NuoC.
InterProiIPR010218. NADH_DH_suC.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01961. NuoC_fam. 1 hit.
PROSITEiPS00542. COMPLEX1_30K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure and expression of a nuclear-coded subunit of complex I homologous to proteins specified by the chloroplast genome."
    Videira A., Tropschug M., Werner S.
    Biochem. Biophys. Res. Commun. 171:1168-1174(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiNDUS3_NEUCR
AccessioniPrimary (citable) accession number: P23710
Secondary accession number(s): Q7RV79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: June 7, 2005
Last modified: January 7, 2015
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.