Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P23710 (NDUS3_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-ubiquinone oxidoreductase 30.4 kDa subunit, mitochondrial

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
CI-31kD
Complex I-30kD
Gene names
Name:nuo-31
ORF Names:NCU04074
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. HAMAP-Rule MF_01357

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out). HAMAP-Rule MF_01357

NADH + acceptor = NAD+ + reduced acceptor. HAMAP-Rule MF_01357

Subunit structure

Complex I is composed of about 40 different subunits. This is a component of the iron-sulfur protein fraction.

Subcellular location

Mitochondrion inner membrane HAMAP-Rule MF_01357.

Sequence similarities

Belongs to the complex I 30 kDa subunit family.

Ontologies

Keywords
   Biological processElectron transport
Respiratory chain
Transport
   Cellular componentMembrane
Mitochondrion
Mitochondrion inner membrane
   DomainTransit peptide
   LigandNAD
Ubiquinone
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmitochondrial inner membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

respiratory chain

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionNADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1717Mitochondrion Potential
Chain18 – 283266NADH-ubiquinone oxidoreductase 30.4 kDa subunit, mitochondrial HAMAP-Rule MF_01357
PRO_0000020000

Experimental info

Sequence conflict401G → R Ref.1

Sequences

Sequence LengthMass (Da)Tools
P23710 [UniParc].

Last modified June 7, 2005. Version 2.
Checksum: 3A2DCD3489F32C6F

FASTA28332,185
        10         20         30         40         50         60 
MASKLCRSRA LASALRSAKP SPAIRCLATT SRNLINMPEG PNPRQFPREP LPGALNAAVV 

        70         80         90        100        110        120 
NPADKYQSKA DNLHKYGSWL MGCLPKYIQQ FSVWKDELTI YISPAGVIPV FSFLKYNTAA 

       130        140        150        160        170        180 
EYTQVSDITA VDFPTKDQRF EVVYNLLSVR HNSRIRVKTY ADEVSPVPSI TPLYDGANWY 

       190        200        210        220        230        240 
EREVYDLFGV FFTGHPDLRR IMTDYGFDGH PLRKDFPMTG YTEIRYDEEK KRIVTEPLEM 

       250        260        270        280 
TQAFRNFEGG SSAWEQVGAG IDRKPESFKL PTPKPETKPE EKK 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure and expression of a nuclear-coded subunit of complex I homologous to proteins specified by the chloroplast genome."
Videira A., Tropschug M., Werner S.
Biochem. Biophys. Res. Commun. 171:1168-1174(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CM002241 Genomic DNA. Translation: EAA28453.1.
PIRA35935.
RefSeqXP_957689.1. XM_952596.2.

3D structure databases

ProteinModelPortalP23710.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU04074.1.

Protein family/group databases

TCDB3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000003652; EFNCRP00000003652; EFNCRG00000003648.
GeneID3873779.
KEGGncr:NCU04074.

Phylogenomic databases

eggNOGCOG0852.
HOGENOMHOG000009797.
KOK03936.
OMALRDHSQC.
OrthoDBEOG7ZGXDG.

Family and domain databases

HAMAPMF_01357. NDH1_NuoC.
InterProIPR010218. NADH_DH_suC.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view]
PfamPF00329. Complex1_30kDa. 1 hit.
[Graphical view]
ProDomPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01961. NuoC_fam. 1 hit.
PROSITEPS00542. COMPLEX1_30K. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDUS3_NEUCR
AccessionPrimary (citable) accession number: P23710
Secondary accession number(s): Q7RV79
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: June 7, 2005
Last modified: February 19, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families