Reviewed, UniProtKB/Swiss-Prot P23710 (NDUS3_NEUCR)

Names and origin

Protein names

Recommended name:
    NADH-ubiquinone oxidoreductase 30.4 kDa subunit, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    Complex I-30kD
    CI-31kD

Gene names

Name:	nuo-31
ORF Names:	NCU04074

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Subunit structure	Complex I is composed of about 40 different subunits. This is a component of the iron-sulfur protein fraction.
Subcellular location	Mitochondrion inner membrane.
Sequence similarities	Belongs to the complex I 30 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 17	17	Mitochondrion Potential
	Chain	18 – 283	266	NADH-ubiquinone oxidoreductase 30.4 kDa subunit, mitochondrial		PRO_0000020000
Experimental info
	Sequence conflict	40	1	G → R Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P23710-1 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: 3A2DCD3489F32C6F	FASTA	283	32,185
	10 20 30 40 50 60 MASKLCRSRA LASALRSAKP SPAIRCLATT SRNLINMPEG PNPRQFPREP LPGALNAAVV 70 80 90 100 110 120 NPADKYQSKA DNLHKYGSWL MGCLPKYIQQ FSVWKDELTI YISPAGVIPV FSFLKYNTAA 130 140 150 160 170 180 EYTQVSDITA VDFPTKDQRF EVVYNLLSVR HNSRIRVKTY ADEVSPVPSI TPLYDGANWY 190 200 210 220 230 240 EREVYDLFGV FFTGHPDLRR IMTDYGFDGH PLRKDFPMTG YTEIRYDEEK KRIVTEPLEM 250 260 270 280 TQAFRNFEGG SSAWEQVGAG IDRKPESFKL PTPKPETKPE EKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure and expression of a nuclear-coded subunit of complex I homologous to proteins specified by the chloroplast genome." Videira A., Tropschug M., Werner S. Biochem. Biophys. Res. Commun. 171:1168-1174(1990) [PubMed: 2145832] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AABX02000012 Genomic DNA. Translation: EAA28453.1.
PIR	A35935.
RefSeq	XP_957689.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.6.2. proton-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
GeneID	3873779.
KEGG	ncr:NCU04074.
NMPDR	fig|5141.1.peg.1326.
Enzyme and pathway databases
BRENDA	1.6.5.3. 266. 1.6.99.3. 266.
Family and domain databases
InterPro	IPR010218. NADH_DH_csu. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS3_NEUCR

Accession

Primary (citable) accession number: P23710
Secondary accession number(s): Q7RV79

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)