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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

Gene

NDUFS3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  2. NADH dehydrogenase activity Source: UniProtKB

GO - Biological processi

  1. negative regulation of cell growth Source: UniProtKB
  2. negative regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
  3. reactive oxygen species metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

NAD, Ubiquinone

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-30kD
Short name:
CI-30kD
NADH-ubiquinone oxidoreductase 30 kDa subunit
Gene namesi
Name:NDUFS3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial membrane Source: UniProtKB
  2. mitochondrial respiratory chain complex I Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3838MitochondrionAdd
BLAST
Chaini39 – 266228NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrialPRO_0000019997Add
BLAST

Proteomic databases

PaxDbiP23709.
PRIDEiP23709.

Interactioni

Subunit structurei

Mammalian complex I is composed of 45 different subunits. Interacts with NDUFAF3 (By similarity).By similarity

Protein-protein interaction databases

IntActiP23709. 3 interactions.
STRINGi9913.ENSBTAP00000024600.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 30 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0852.
HOGENOMiHOG000009797.
HOVERGENiHBG000450.
InParanoidiP23709.
KOiK03936.

Family and domain databases

HAMAPiMF_01357. NDH1_NuoC.
InterProiIPR010218. NADH_DH_suC.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01961. NuoC_fam. 1 hit.
PROSITEiPS00542. COMPLEX1_30K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23709-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAVAAAAR GCWQRLVGSA APARVAGRPS VLLLPVRRES SAADTRPTVR
60 70 80 90 100
PRNDVAHKQL SAFGEYVAEI LPKYVQQVQV SCFNELEICI HPDGVIPVLT
110 120 130 140 150
FLRDHSNAQF KSLADLTAVD IPTRQNRFEI VYNLLSLRFN SRIRVKTYTD
160 170 180 190 200
ELTPIESSVP VYKAANWYER EIWDMFGVFF ANHPDLRRIL TDYGFEGHPF
210 220 230 240 250
RKDFPLSGYV ELRYDDEVKR VVAEPVELAQ EFRKFDLNSP WEAFPAYRQP
260
PESLKLEAGD TKPEAK
Length:266
Mass (Da):30,284
Last modified:November 1, 1991 - v1
Checksum:i43494B3359D2FEB1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti254 – 2541L → E AA sequence (PubMed:1907966).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58469 mRNA. Translation: AAA30663.1. Sequence problems.
PIRiA37957.
RefSeqiNP_777244.1. NM_174819.3.
UniGeneiBt.1153.

Genome annotation databases

GeneIDi287327.
KEGGibta:287327.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58469 mRNA. Translation: AAA30663.1. Sequence problems.
PIRiA37957.
RefSeqiNP_777244.1. NM_174819.3.
UniGeneiBt.1153.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP23709. 3 interactions.
STRINGi9913.ENSBTAP00000024600.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP23709.
PRIDEiP23709.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi287327.
KEGGibta:287327.

Organism-specific databases

CTDi4722.

Phylogenomic databases

eggNOGiCOG0852.
HOGENOMiHOG000009797.
HOVERGENiHBG000450.
InParanoidiP23709.
KOiK03936.

Miscellaneous databases

NextBioi20806550.

Family and domain databases

HAMAPiMF_01357. NDH1_NuoC.
InterProiIPR010218. NADH_DH_suC.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view]
PfamiPF00329. Complex1_30kDa. 1 hit.
[Graphical view]
ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01961. NuoC_fam. 1 hit.
PROSITEiPS00542. COMPLEX1_30K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The 30-kilodalton subunit of bovine mitochondrial complex I is homologous to a protein coded in chloroplast DNA."
    Pilkington S.J., Skehel J.M., Walker J.E.
    Biochemistry 30:1901-1908(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "The amino acid sequences of two 13 kDa polypeptides and partial amino acid sequence of 30 kDa polypeptide of complex I from bovine heart mitochondria: possible location of iron-sulfur clusters."
    Masui R., Wakabayashi S., Matsubara H., Hatefi Y.
    J. Biochem. 109:534-543(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 59-99 AND 197-266.
    Tissue: Heart.

Entry informationi

Entry nameiNDUS3_BOVIN
AccessioniPrimary (citable) accession number: P23709
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 1, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.