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P23709

- NDUS3_BOVIN

UniProt

P23709 - NDUS3_BOVIN

Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

Gene

NDUFS3

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    GO - Molecular functioni

    1. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
    2. NADH dehydrogenase activity Source: UniProtKB

    GO - Biological processi

    1. negative regulation of cell growth Source: UniProtKB
    2. negative regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
    3. reactive oxygen species metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    NAD, Ubiquinone

    Protein family/group databases

    TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-30kD
    Short name:
    CI-30kD
    NADH-ubiquinone oxidoreductase 30 kDa subunit
    Gene namesi
    Name:NDUFS3
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial membrane Source: UniProtKB
    2. mitochondrial respiratory chain complex I Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3838MitochondrionAdd
    BLAST
    Chaini39 – 266228NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrialPRO_0000019997Add
    BLAST

    Proteomic databases

    PaxDbiP23709.
    PRIDEiP23709.

    Interactioni

    Subunit structurei

    Mammalian complex I is composed of 45 different subunits. Interacts with NDUFAF3 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP23709. 3 interactions.
    STRINGi9913.ENSBTAP00000024600.

    Structurei

    3D structure databases

    ProteinModelPortaliP23709.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 30 kDa subunit family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0852.
    HOGENOMiHOG000009797.
    HOVERGENiHBG000450.
    InParanoidiP23709.
    KOiK03936.

    Family and domain databases

    HAMAPiMF_01357. NDH1_NuoC.
    InterProiIPR010218. NADH_DH_suC.
    IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
    IPR020396. NADH_UbQ_OxRdtase_CS.
    [Graphical view]
    PfamiPF00329. Complex1_30kDa. 1 hit.
    [Graphical view]
    ProDomiPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    TIGRFAMsiTIGR01961. NuoC_fam. 1 hit.
    PROSITEiPS00542. COMPLEX1_30K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23709-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAVAAAAR GCWQRLVGSA APARVAGRPS VLLLPVRRES SAADTRPTVR    50
    PRNDVAHKQL SAFGEYVAEI LPKYVQQVQV SCFNELEICI HPDGVIPVLT 100
    FLRDHSNAQF KSLADLTAVD IPTRQNRFEI VYNLLSLRFN SRIRVKTYTD 150
    ELTPIESSVP VYKAANWYER EIWDMFGVFF ANHPDLRRIL TDYGFEGHPF 200
    RKDFPLSGYV ELRYDDEVKR VVAEPVELAQ EFRKFDLNSP WEAFPAYRQP 250
    PESLKLEAGD TKPEAK 266
    Length:266
    Mass (Da):30,284
    Last modified:November 1, 1991 - v1
    Checksum:i43494B3359D2FEB1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti254 – 2541L → E AA sequence (PubMed:1907966)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58469 mRNA. Translation: AAA30663.1. Sequence problems.
    PIRiA37957.
    RefSeqiNP_777244.1. NM_174819.3.
    UniGeneiBt.1153.

    Genome annotation databases

    GeneIDi287327.
    KEGGibta:287327.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58469 mRNA. Translation: AAA30663.1 . Sequence problems.
    PIRi A37957.
    RefSeqi NP_777244.1. NM_174819.3.
    UniGenei Bt.1153.

    3D structure databases

    ProteinModelPortali P23709.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P23709. 3 interactions.
    STRINGi 9913.ENSBTAP00000024600.

    Protein family/group databases

    TCDBi 3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Proteomic databases

    PaxDbi P23709.
    PRIDEi P23709.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 287327.
    KEGGi bta:287327.

    Organism-specific databases

    CTDi 4722.

    Phylogenomic databases

    eggNOGi COG0852.
    HOGENOMi HOG000009797.
    HOVERGENi HBG000450.
    InParanoidi P23709.
    KOi K03936.

    Miscellaneous databases

    NextBioi 20806550.

    Family and domain databases

    HAMAPi MF_01357. NDH1_NuoC.
    InterProi IPR010218. NADH_DH_suC.
    IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
    IPR020396. NADH_UbQ_OxRdtase_CS.
    [Graphical view ]
    Pfami PF00329. Complex1_30kDa. 1 hit.
    [Graphical view ]
    ProDomi PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    TIGRFAMsi TIGR01961. NuoC_fam. 1 hit.
    PROSITEi PS00542. COMPLEX1_30K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The 30-kilodalton subunit of bovine mitochondrial complex I is homologous to a protein coded in chloroplast DNA."
      Pilkington S.J., Skehel J.M., Walker J.E.
      Biochemistry 30:1901-1908(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "The amino acid sequences of two 13 kDa polypeptides and partial amino acid sequence of 30 kDa polypeptide of complex I from bovine heart mitochondria: possible location of iron-sulfur clusters."
      Masui R., Wakabayashi S., Matsubara H., Hatefi Y.
      J. Biochem. 109:534-543(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 59-99 AND 197-266.
      Tissue: Heart.

    Entry informationi

    Entry nameiNDUS3_BOVIN
    AccessioniPrimary (citable) accession number: P23709
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3