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P23709 (NDUS3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
Complex I-30kD
Short name=CI-30kD
NADH-ubiquinone oxidoreductase 30 kDa subunit
Gene names
Name:NDUFS3
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. HAMAP-Rule MF_01357

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out). HAMAP-Rule MF_01357

NADH + acceptor = NAD+ + reduced acceptor. HAMAP-Rule MF_01357

Subunit structure

Mammalian complex I is composed of 45 different subunits. Interacts with NDUFAF3 By similarity.

Subcellular location

Mitochondrion inner membrane HAMAP-Rule MF_01357.

Sequence similarities

Belongs to the complex I 30 kDa subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3838Mitochondrion HAMAP-Rule MF_01357
Chain39 – 266228NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial HAMAP-Rule MF_01357
PRO_0000019997

Experimental info

Sequence conflict2541L → E AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P23709 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 43494B3359D2FEB1

FASTA26630,284
        10         20         30         40         50         60 
MAAAVAAAAR GCWQRLVGSA APARVAGRPS VLLLPVRRES SAADTRPTVR PRNDVAHKQL 

        70         80         90        100        110        120 
SAFGEYVAEI LPKYVQQVQV SCFNELEICI HPDGVIPVLT FLRDHSNAQF KSLADLTAVD 

       130        140        150        160        170        180 
IPTRQNRFEI VYNLLSLRFN SRIRVKTYTD ELTPIESSVP VYKAANWYER EIWDMFGVFF 

       190        200        210        220        230        240 
ANHPDLRRIL TDYGFEGHPF RKDFPLSGYV ELRYDDEVKR VVAEPVELAQ EFRKFDLNSP 

       250        260 
WEAFPAYRQP PESLKLEAGD TKPEAK 

« Hide

References

[1]"The 30-kilodalton subunit of bovine mitochondrial complex I is homologous to a protein coded in chloroplast DNA."
Pilkington S.J., Skehel J.M., Walker J.E.
Biochemistry 30:1901-1908(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"The amino acid sequences of two 13 kDa polypeptides and partial amino acid sequence of 30 kDa polypeptide of complex I from bovine heart mitochondria: possible location of iron-sulfur clusters."
Masui R., Wakabayashi S., Matsubara H., Hatefi Y.
J. Biochem. 109:534-543(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 59-99 AND 197-266.
Tissue: Heart.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M58469 mRNA. Translation: AAA30663.1. Sequence problems.
PIRA37957.
RefSeqNP_777244.1. NM_174819.3.
UniGeneBt.1153.

3D structure databases

ProteinModelPortalP23709.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP23709. 3 interactions.
STRING9913.ENSBTAP00000024600.

Protein family/group databases

TCDB3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbP23709.
PRIDEP23709.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID287327.
KEGGbta:287327.

Organism-specific databases

CTD4722.

Phylogenomic databases

eggNOGCOG0852.
HOGENOMHOG000009797.
HOVERGENHBG000450.
InParanoidP23709.
KOK03936.

Family and domain databases

HAMAPMF_01357. NDH1_NuoC.
InterProIPR010218. NADH_DH_suC.
IPR001268. NADH_UbQ_OxRdtase_30kDa_su.
IPR020396. NADH_UbQ_OxRdtase_CS.
[Graphical view]
PfamPF00329. Complex1_30kDa. 1 hit.
[Graphical view]
ProDomPD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01961. NuoC_fam. 1 hit.
PROSITEPS00542. COMPLEX1_30K. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806550.

Entry information

Entry nameNDUS3_BOVIN
AccessionPrimary (citable) accession number: P23709
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families