Reviewed, UniProtKB/Swiss-Prot P23709 (NDUS3_BOVIN)

Names and origin

Protein names

Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH-ubiquinone oxidoreductase 30 kDa subunit
    Complex I-30kD
      Short name=CI-30kD

Gene names

Name:

NDUFS3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Subunit structure	Mammalian complex I is composed of 45 different subunits.
Subcellular location	Mitochondrion inner membrane.
Sequence similarities	Belongs to the complex I 30 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW induction of apoptosis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell growth Inferred from sequence or structural similarity. Source: UniProtKB oxygen and reactive oxygen species metabolic process Inferred from sequence or structural similarity. Source: UniProtKB transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain complex I Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 38	38	Mitochondrion
	Chain	39 – 266	228	NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial		PRO_0000019997
Experimental info
	Sequence conflict	254	1	L → E AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P23709-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 43494B3359D2FEB1	FASTA	266	30,284
	10 20 30 40 50 60 MAAAVAAAAR GCWQRLVGSA APARVAGRPS VLLLPVRRES SAADTRPTVR PRNDVAHKQL 70 80 90 100 110 120 SAFGEYVAEI LPKYVQQVQV SCFNELEICI HPDGVIPVLT FLRDHSNAQF KSLADLTAVD 130 140 150 160 170 180 IPTRQNRFEI VYNLLSLRFN SRIRVKTYTD ELTPIESSVP VYKAANWYER EIWDMFGVFF 190 200 210 220 230 240 ANHPDLRRIL TDYGFEGHPF RKDFPLSGYV ELRYDDEVKR VVAEPVELAQ EFRKFDLNSP 250 260 WEAFPAYRQP PESLKLEAGD TKPEAK

References

[1]	"The 30-kilodalton subunit of bovine mitochondrial complex I is homologous to a protein coded in chloroplast DNA." Pilkington S.J., Skehel J.M., Walker J.E. Biochemistry 30:1901-1908(1991) [PubMed: 1899621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The amino acid sequences of two 13 kDa polypeptides and partial amino acid sequence of 30 kDa polypeptide of complex I from bovine heart mitochondria: possible location of iron-sulfur clusters." Masui R., Wakabayashi S., Matsubara H., Hatefi Y. J. Biochem. 109:534-543(1991) [PubMed: 1907966] [Abstract] Cited for: PROTEIN SEQUENCE OF 59-99 AND 197-266. Tissue: Heart.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M58469 mRNA. Translation: AAA30663.1. Sequence problems.
IPI	IPI00686077.
PIR	A37957.
RefSeq	NP_777244.1.
UniGene	Bt.1153
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.6.1. proton-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
Ensembl	ENSBTAG00000018483. Bos taurus. [Contig view]
GeneID	287327.
KEGG	bta:287327.
Phylogenomic databases
HOVERGEN	P23709.
Enzyme and pathway databases
BRENDA	1.6.5.3. 251. 1.6.99.3. 251.
Family and domain databases
InterPro	IPR010218. NADH_DH_csu. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS3_BOVIN

Accession

Primary (citable) accession number: P23709

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)