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P23708

- NFYA_MOUSE

UniProt

P23708 - NFYA_MOUSE

Protein

Nuclear transcription factor Y subunit alpha

Gene

Nfya

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (01 May 1992)
      Previous versions | rss
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    Functioni

    Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi295 – 32026NFYA/HAP2-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. core promoter sequence-specific DNA binding Source: UniProtKB
    2. DNA binding Source: MGI
    3. protein binding Source: UniProtKB
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
    5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    6. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. positive regulation of stem cell proliferation Source: MGI
    2. positive regulation of transcription, DNA-templated Source: UniProtKB
    3. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    4. regulation of stem cell maintenance Source: MGI

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Biological rhythms, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198602. PPARA activates gene expression.
    REACT_198969. Activation of gene expression by SREBF (SREBP).
    REACT_32838. ATF6-alpha activates chaperone genes.
    REACT_90370. ATF4 activates genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear transcription factor Y subunit alpha
    Alternative name(s):
    CAAT box DNA-binding protein subunit A
    Nuclear transcription factor Y subunit A
    Short name:
    NF-YA
    Gene namesi
    Name:Nfya
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:97316. Nfya.

    Subcellular locationi

    GO - Cellular componenti

    1. CCAAT-binding factor complex Source: InterPro
    2. nucleoplasm Source: Reactome
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 346346Nuclear transcription factor Y subunit alphaPRO_0000198769Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei325 – 3251PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP23708.
    PRIDEiP23708.

    PTM databases

    PhosphoSiteiP23708.

    Expressioni

    Gene expression databases

    ArrayExpressiP23708.
    BgeeiP23708.
    CleanExiMM_NFYA.
    GenevestigatoriP23708.

    Interactioni

    Subunit structurei

    Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding By similarity. Interacts with SP1; the interaction is inhibited by glycosylation of SP1. Interacts (via N-terminus) with ZHX2 (via homeobox domain). Interacts with ZFX3. Interacts with ZHX1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SP1P0804718EBI-862337,EBI-298336From a different organism.

    Protein-protein interaction databases

    BioGridi201760. 4 interactions.
    DIPiDIP-2661N.
    IntActiP23708. 2 interactions.
    MINTiMINT-4089997.

    Structurei

    3D structure databases

    ProteinModelPortaliP23708.
    SMRiP23708. Positions 261-321.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi265 – 28824Subunit association domain (SAD)Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi14 – 160147Gln-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NFYA/HAP2 subunit family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5224.
    GeneTreeiENSGT00390000015714.
    HOGENOMiHOG000247016.
    HOVERGENiHBG005253.
    InParanoidiP23708.
    KOiK08064.
    OrthoDBiEOG7MD4RM.
    PhylomeDBiP23708.

    Family and domain databases

    InterProiIPR018362. CCAAT-binding_factor_CS.
    IPR001289. TF_CBFB.
    [Graphical view]
    PANTHERiPTHR12632. PTHR12632. 1 hit.
    PfamiPF02045. CBFB_NFYA. 1 hit.
    [Graphical view]
    PRINTSiPR00616. CCAATSUBUNTB.
    SMARTiSM00521. CBF. 1 hit.
    [Graphical view]
    PROSITEiPS00686. NFYA_HAP2_1. 1 hit.
    PS51152. NFYA_HAP2_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P23708-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEQYTTNSNS STEQIVVQAG QIQQQQGGVT AVQLQTEAQV ASASGQQVQT    50
    LQVVQGQPLM VQVSGGQLIT STGQPIMVQA VPGGQGQTIM QVPVSGTQGL 100
    QQIQLVPPGQ IQIQGGQAVQ VQGQQGQTQQ IIIQQPQTAV TAGQTQTQQQ 150
    IAVQGQQVAQ TAEGQTIVYQ PVNADGTILQ QVTVPVSGMI TIPAASLAGA 200
    QIVQTGANTN TTSSGQGTVT VTLPVAGNVV NSGGMVMMVP GAGSVPAIQR 250
    IPLPGAEMLE EEPLYVNAKQ YHRILKRRQA RAKLEAEGKI PKERRKYLHE 300
    SRHRHAMARK RGEGGRFFSP KEKDSPHMQD PNQADEEAMT QIIRVS 346
    Length:346
    Mass (Da):36,779
    Last modified:May 1, 1992 - v2
    Checksum:i1291AA0D3AA76E05
    GO
    Isoform Short (identifier: P23708-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         26-53: Missing.

    Show »
    Length:318
    Mass (Da):33,969
    Checksum:iD3807C653CA04D85
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti182 – 1876Missing in AAA39816. (PubMed:1577736)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei26 – 5328Missing in isoform Short. CuratedVSP_000850Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55315 mRNA. Translation: CAA39023.1.
    M86215 mRNA. Translation: AAA39817.1.
    M86214 mRNA. Translation: AAA39816.1.
    CCDSiCCDS28867.1. [P23708-2]
    PIRiA38245.
    C38245.
    D38245.
    E38245.
    RefSeqiNP_035043.1. NM_010913.2. [P23708-2]
    XP_006523854.1. XM_006523791.1. [P23708-1]
    XP_006523859.1. XM_006523796.1.
    UniGeneiMm.4929.

    Genome annotation databases

    EnsembliENSMUST00000078800; ENSMUSP00000077853; ENSMUSG00000023994. [P23708-1]
    ENSMUST00000159063; ENSMUSP00000124404; ENSMUSG00000023994.
    ENSMUST00000162460; ENSMUSP00000123785; ENSMUSG00000023994. [P23708-2]
    GeneIDi18044.
    KEGGimmu:18044.
    UCSCiuc008cxp.2. mouse. [P23708-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55315 mRNA. Translation: CAA39023.1 .
    M86215 mRNA. Translation: AAA39817.1 .
    M86214 mRNA. Translation: AAA39816.1 .
    CCDSi CCDS28867.1. [P23708-2 ]
    PIRi A38245.
    C38245.
    D38245.
    E38245.
    RefSeqi NP_035043.1. NM_010913.2. [P23708-2 ]
    XP_006523854.1. XM_006523791.1. [P23708-1 ]
    XP_006523859.1. XM_006523796.1.
    UniGenei Mm.4929.

    3D structure databases

    ProteinModelPortali P23708.
    SMRi P23708. Positions 261-321.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201760. 4 interactions.
    DIPi DIP-2661N.
    IntActi P23708. 2 interactions.
    MINTi MINT-4089997.

    PTM databases

    PhosphoSitei P23708.

    Proteomic databases

    PaxDbi P23708.
    PRIDEi P23708.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000078800 ; ENSMUSP00000077853 ; ENSMUSG00000023994 . [P23708-1 ]
    ENSMUST00000159063 ; ENSMUSP00000124404 ; ENSMUSG00000023994 .
    ENSMUST00000162460 ; ENSMUSP00000123785 ; ENSMUSG00000023994 . [P23708-2 ]
    GeneIDi 18044.
    KEGGi mmu:18044.
    UCSCi uc008cxp.2. mouse. [P23708-1 ]

    Organism-specific databases

    CTDi 4800.
    MGIi MGI:97316. Nfya.

    Phylogenomic databases

    eggNOGi COG5224.
    GeneTreei ENSGT00390000015714.
    HOGENOMi HOG000247016.
    HOVERGENi HBG005253.
    InParanoidi P23708.
    KOi K08064.
    OrthoDBi EOG7MD4RM.
    PhylomeDBi P23708.

    Enzyme and pathway databases

    Reactomei REACT_198602. PPARA activates gene expression.
    REACT_198969. Activation of gene expression by SREBF (SREBP).
    REACT_32838. ATF6-alpha activates chaperone genes.
    REACT_90370. ATF4 activates genes.

    Miscellaneous databases

    NextBioi 293157.
    PROi P23708.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P23708.
    Bgeei P23708.
    CleanExi MM_NFYA.
    Genevestigatori P23708.

    Family and domain databases

    InterProi IPR018362. CCAAT-binding_factor_CS.
    IPR001289. TF_CBFB.
    [Graphical view ]
    PANTHERi PTHR12632. PTHR12632. 1 hit.
    Pfami PF02045. CBFB_NFYA. 1 hit.
    [Graphical view ]
    PRINTSi PR00616. CCAATSUBUNTB.
    SMARTi SM00521. CBF. 1 hit.
    [Graphical view ]
    PROSITEi PS00686. NFYA_HAP2_1. 1 hit.
    PS51152. NFYA_HAP2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolutionary variation of the CCAAT-binding transcription factor NF-Y."
      Li X.-Y., Mantovani R., Hooft van Huijsduijnen R., Andre I., Benoist C., Mathis D.
      Nucleic Acids Res. 20:1087-1091(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Intron-exon organization of the NF-Y genes. Tissue-specific splicing modifies an activation domain."
      Li X.-Y., Hooft van Huijsduijnen R., Mantovani R., Benoist C.O., Mathis D.
      J. Biol. Chem. 267:8984-8990(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Co-evolution from yeast to mouse: cDNA cloning of the two NF-Y (CP-1/CBF) subunits."
      Hooft van Huijsduijnen R., Li X.-Y., Black D., Matthes H., Benoist C., Mathis D.
      EMBO J. 9:3119-3127(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-25 AND 54-346, PARTIAL PROTEIN SEQUENCE.
    4. "Transcription factor NF-Y is a functional regulator of the transcription of core clock gene Bmal1."
      Xiao J., Zhou Y., Lai H., Lei S., Chi L.H., Mo X.
      J. Biol. Chem. 288:31930-31936(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiNFYA_MOUSE
    AccessioniPrimary (citable) accession number: P23708
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3