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Protein

Nuclear transcription factor Y subunit alpha

Gene

Nfya

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi295 – 320NFYA/HAP2-typePROSITE-ProRule annotationAdd BLAST26

GO - Molecular functioni

  • core promoter sequence-specific DNA binding Source: UniProtKB
  • DNA binding Source: MGI
  • DNA binding transcription factor activity Source: MGI
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: MGI
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB

GO - Biological processi

  • positive regulation of stem cell proliferation Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: BHF-UCL
  • regulation of stem cell population maintenance Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • rhythmic process Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processBiological rhythms, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transcription factor Y subunit alpha
Alternative name(s):
CAAT box DNA-binding protein subunit A
Nuclear transcription factor Y subunit A
Short name:
NF-YA
Gene namesi
Name:Nfya
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:97316 Nfya

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001987691 – 346Nuclear transcription factor Y subunit alphaAdd BLAST346

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei325PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23708
PaxDbiP23708
PRIDEiP23708

PTM databases

iPTMnetiP23708
PhosphoSitePlusiP23708

Expressioni

Gene expression databases

BgeeiENSMUSG00000023994
CleanExiMM_NFYA
ExpressionAtlasiP23708 baseline and differential
GenevisibleiP23708 MM

Interactioni

Subunit structurei

Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding (By similarity). Interacts with SP1; the interaction is inhibited by glycosylation of SP1. Interacts (via N-terminus) with ZHX2 (via homeobox domain). Interacts with ZFX3. Interacts with ZHX1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
SP1P0804718EBI-862337,EBI-298336From Homo sapiens.

Protein-protein interaction databases

BioGridi201760, 17 interactors
DIPiDIP-2661N
IntActiP23708, 16 interactors
MINTiP23708
STRINGi10090.ENSMUSP00000043909

Structurei

3D structure databases

ProteinModelPortaliP23708
SMRiP23708
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi265 – 288Subunit association domain (SAD)Add BLAST24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 160Gln-richAdd BLAST147

Sequence similaritiesi

Belongs to the NFYA/HAP2 subunit family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1561 Eukaryota
COG5224 LUCA
GeneTreeiENSGT00390000015714
HOGENOMiHOG000247016
HOVERGENiHBG005253
InParanoidiP23708
KOiK08064
PhylomeDBiP23708

Family and domain databases

InterProiView protein in InterPro
IPR018362 CCAAT-binding_factor_CS
IPR001289 NFYA
PANTHERiPTHR12632 PTHR12632, 1 hit
PfamiView protein in Pfam
PF02045 CBFB_NFYA, 1 hit
PRINTSiPR00616 CCAATSUBUNTB
SMARTiView protein in SMART
SM00521 CBF, 1 hit
PROSITEiView protein in PROSITE
PS00686 NFYA_HAP2_1, 1 hit
PS51152 NFYA_HAP2_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P23708-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEQYTTNSNS STEQIVVQAG QIQQQQGGVT AVQLQTEAQV ASASGQQVQT
60 70 80 90 100
LQVVQGQPLM VQVSGGQLIT STGQPIMVQA VPGGQGQTIM QVPVSGTQGL
110 120 130 140 150
QQIQLVPPGQ IQIQGGQAVQ VQGQQGQTQQ IIIQQPQTAV TAGQTQTQQQ
160 170 180 190 200
IAVQGQQVAQ TAEGQTIVYQ PVNADGTILQ QVTVPVSGMI TIPAASLAGA
210 220 230 240 250
QIVQTGANTN TTSSGQGTVT VTLPVAGNVV NSGGMVMMVP GAGSVPAIQR
260 270 280 290 300
IPLPGAEMLE EEPLYVNAKQ YHRILKRRQA RAKLEAEGKI PKERRKYLHE
310 320 330 340
SRHRHAMARK RGEGGRFFSP KEKDSPHMQD PNQADEEAMT QIIRVS
Length:346
Mass (Da):36,779
Last modified:May 1, 1992 - v2
Checksum:i1291AA0D3AA76E05
GO
Isoform Short (identifier: P23708-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     26-53: Missing.

Show »
Length:318
Mass (Da):33,969
Checksum:iD3807C653CA04D85
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182 – 187Missing in AAA39816 (PubMed:1577736).Curated6

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00085026 – 53Missing in isoform Short. CuratedAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55315 mRNA Translation: CAA39023.1
M86215 mRNA Translation: AAA39817.1
M86214 mRNA Translation: AAA39816.1
CCDSiCCDS28867.1 [P23708-2]
CCDS84321.1 [P23708-1]
PIRiA38245
C38245
D38245
E38245
RefSeqiNP_001334330.1, NM_001347401.1 [P23708-1]
NP_001334331.1, NM_001347402.1
NP_035043.1, NM_010913.2 [P23708-2]
UniGeneiMm.4929

Genome annotation databases

EnsembliENSMUST00000078800; ENSMUSP00000077853; ENSMUSG00000023994 [P23708-1]
ENSMUST00000162460; ENSMUSP00000123785; ENSMUSG00000023994 [P23708-2]
GeneIDi18044
KEGGimmu:18044
UCSCiuc008cxp.2 mouse [P23708-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNFYA_MOUSE
AccessioniPrimary (citable) accession number: P23708
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: May 1, 1992
Last modified: March 28, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health