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Protein

Nuclear transcription factor Y subunit alpha

Gene

Nfya

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi295 – 32026NFYA/HAP2-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. core promoter sequence-specific DNA binding Source: UniProtKB
  2. DNA binding Source: MGI
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  5. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. positive regulation of stem cell proliferation Source: MGI
  2. positive regulation of transcription, DNA-templated Source: UniProtKB
  3. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  4. regulation of stem cell maintenance Source: MGI
  5. regulation of transcription, DNA-templated Source: MGI
  6. rhythmic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_272139. PPARA activates gene expression.
REACT_303828. Activation of gene expression by SREBF (SREBP).

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transcription factor Y subunit alpha
Alternative name(s):
CAAT box DNA-binding protein subunit A
Nuclear transcription factor Y subunit A
Short name:
NF-YA
Gene namesi
Name:Nfya
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:97316. Nfya.

Subcellular locationi

GO - Cellular componenti

  1. CCAAT-binding factor complex Source: MGI
  2. nucleoplasm Source: Reactome
  3. nucleus Source: MGI
  4. protein-DNA complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346Nuclear transcription factor Y subunit alphaPRO_0000198769Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei325 – 3251PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23708.
PaxDbiP23708.
PRIDEiP23708.

PTM databases

PhosphoSiteiP23708.

Expressioni

Gene expression databases

BgeeiP23708.
CleanExiMM_NFYA.
ExpressionAtlasiP23708. baseline and differential.
GenevestigatoriP23708.

Interactioni

Subunit structurei

Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding (By similarity). Interacts with SP1; the interaction is inhibited by glycosylation of SP1. Interacts (via N-terminus) with ZHX2 (via homeobox domain). Interacts with ZFX3. Interacts with ZHX1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
SP1P0804718EBI-862337,EBI-298336From a different organism.

Protein-protein interaction databases

BioGridi201760. 4 interactions.
DIPiDIP-2661N.
IntActiP23708. 3 interactions.
MINTiMINT-4089997.

Structurei

3D structure databases

SMRiP23708. Positions 261-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi265 – 28824Subunit association domain (SAD)Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi14 – 160147Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the NFYA/HAP2 subunit family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5224.
GeneTreeiENSGT00390000015714.
HOGENOMiHOG000247016.
HOVERGENiHBG005253.
InParanoidiP23708.
KOiK08064.
OrthoDBiEOG7MD4RM.
PhylomeDBiP23708.

Family and domain databases

InterProiIPR018362. CCAAT-binding_factor_CS.
IPR001289. TF_CBFB.
[Graphical view]
PANTHERiPTHR12632. PTHR12632. 1 hit.
PfamiPF02045. CBFB_NFYA. 1 hit.
[Graphical view]
PRINTSiPR00616. CCAATSUBUNTB.
SMARTiSM00521. CBF. 1 hit.
[Graphical view]
PROSITEiPS00686. NFYA_HAP2_1. 1 hit.
PS51152. NFYA_HAP2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P23708-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEQYTTNSNS STEQIVVQAG QIQQQQGGVT AVQLQTEAQV ASASGQQVQT
60 70 80 90 100
LQVVQGQPLM VQVSGGQLIT STGQPIMVQA VPGGQGQTIM QVPVSGTQGL
110 120 130 140 150
QQIQLVPPGQ IQIQGGQAVQ VQGQQGQTQQ IIIQQPQTAV TAGQTQTQQQ
160 170 180 190 200
IAVQGQQVAQ TAEGQTIVYQ PVNADGTILQ QVTVPVSGMI TIPAASLAGA
210 220 230 240 250
QIVQTGANTN TTSSGQGTVT VTLPVAGNVV NSGGMVMMVP GAGSVPAIQR
260 270 280 290 300
IPLPGAEMLE EEPLYVNAKQ YHRILKRRQA RAKLEAEGKI PKERRKYLHE
310 320 330 340
SRHRHAMARK RGEGGRFFSP KEKDSPHMQD PNQADEEAMT QIIRVS
Length:346
Mass (Da):36,779
Last modified:April 30, 1992 - v2
Checksum:i1291AA0D3AA76E05
GO
Isoform Short (identifier: P23708-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     26-53: Missing.

Show »
Length:318
Mass (Da):33,969
Checksum:iD3807C653CA04D85
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 1876Missing in AAA39816 (PubMed:1577736).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei26 – 5328Missing in isoform Short. CuratedVSP_000850Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55315 mRNA. Translation: CAA39023.1.
M86215 mRNA. Translation: AAA39817.1.
M86214 mRNA. Translation: AAA39816.1.
CCDSiCCDS28867.1. [P23708-2]
PIRiA38245.
C38245.
D38245.
E38245.
RefSeqiNP_035043.1. NM_010913.2. [P23708-2]
XP_006523854.1. XM_006523791.1. [P23708-1]
XP_006523859.1. XM_006523796.1.
UniGeneiMm.4929.

Genome annotation databases

EnsembliENSMUST00000078800; ENSMUSP00000077853; ENSMUSG00000023994. [P23708-1]
ENSMUST00000162460; ENSMUSP00000123785; ENSMUSG00000023994. [P23708-2]
GeneIDi18044.
KEGGimmu:18044.
UCSCiuc008cxp.2. mouse. [P23708-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55315 mRNA. Translation: CAA39023.1.
M86215 mRNA. Translation: AAA39817.1.
M86214 mRNA. Translation: AAA39816.1.
CCDSiCCDS28867.1. [P23708-2]
PIRiA38245.
C38245.
D38245.
E38245.
RefSeqiNP_035043.1. NM_010913.2. [P23708-2]
XP_006523854.1. XM_006523791.1. [P23708-1]
XP_006523859.1. XM_006523796.1.
UniGeneiMm.4929.

3D structure databases

SMRiP23708. Positions 261-321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201760. 4 interactions.
DIPiDIP-2661N.
IntActiP23708. 3 interactions.
MINTiMINT-4089997.

PTM databases

PhosphoSiteiP23708.

Proteomic databases

MaxQBiP23708.
PaxDbiP23708.
PRIDEiP23708.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078800; ENSMUSP00000077853; ENSMUSG00000023994. [P23708-1]
ENSMUST00000162460; ENSMUSP00000123785; ENSMUSG00000023994. [P23708-2]
GeneIDi18044.
KEGGimmu:18044.
UCSCiuc008cxp.2. mouse. [P23708-1]

Organism-specific databases

CTDi4800.
MGIiMGI:97316. Nfya.

Phylogenomic databases

eggNOGiCOG5224.
GeneTreeiENSGT00390000015714.
HOGENOMiHOG000247016.
HOVERGENiHBG005253.
InParanoidiP23708.
KOiK08064.
OrthoDBiEOG7MD4RM.
PhylomeDBiP23708.

Enzyme and pathway databases

ReactomeiREACT_272139. PPARA activates gene expression.
REACT_303828. Activation of gene expression by SREBF (SREBP).

Miscellaneous databases

NextBioi293157.
PROiP23708.
SOURCEiSearch...

Gene expression databases

BgeeiP23708.
CleanExiMM_NFYA.
ExpressionAtlasiP23708. baseline and differential.
GenevestigatoriP23708.

Family and domain databases

InterProiIPR018362. CCAAT-binding_factor_CS.
IPR001289. TF_CBFB.
[Graphical view]
PANTHERiPTHR12632. PTHR12632. 1 hit.
PfamiPF02045. CBFB_NFYA. 1 hit.
[Graphical view]
PRINTSiPR00616. CCAATSUBUNTB.
SMARTiSM00521. CBF. 1 hit.
[Graphical view]
PROSITEiPS00686. NFYA_HAP2_1. 1 hit.
PS51152. NFYA_HAP2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Evolutionary variation of the CCAAT-binding transcription factor NF-Y."
    Li X.-Y., Mantovani R., Hooft van Huijsduijnen R., Andre I., Benoist C., Mathis D.
    Nucleic Acids Res. 20:1087-1091(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Intron-exon organization of the NF-Y genes. Tissue-specific splicing modifies an activation domain."
    Li X.-Y., Hooft van Huijsduijnen R., Mantovani R., Benoist C.O., Mathis D.
    J. Biol. Chem. 267:8984-8990(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Co-evolution from yeast to mouse: cDNA cloning of the two NF-Y (CP-1/CBF) subunits."
    Hooft van Huijsduijnen R., Li X.-Y., Black D., Matthes H., Benoist C., Mathis D.
    EMBO J. 9:3119-3127(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-25 AND 54-346, PARTIAL PROTEIN SEQUENCE.
  4. "Transcription factor NF-Y is a functional regulator of the transcription of core clock gene Bmal1."
    Xiao J., Zhou Y., Lai H., Lei S., Chi L.H., Mo X.
    J. Biol. Chem. 288:31930-31936(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNFYA_MOUSE
AccessioniPrimary (citable) accession number: P23708
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1991
Last sequence update: April 30, 1992
Last modified: March 31, 2015
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.