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Protein

Troponin I, cardiac muscle

Gene

Tnni3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei81Involved in TNI-TNT interactions1
Sitei98Involved in TNI-TNT interactions1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi138 – 149By similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-390522. Striated Muscle Contraction.
R-RNO-5578775. Ion homeostasis.

Names & Taxonomyi

Protein namesi
Recommended name:
Troponin I, cardiac muscle
Alternative name(s):
Cardiac troponin I
Gene namesi
Name:Tnni3
Synonyms:Ctni, Tni
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi62052. Tnni3.

Subcellular locationi

GO - Cellular componenti

  • contractile fiber Source: RGD
  • cytoplasm Source: MGI
  • myofibril Source: RGD
  • sarcomere Source: MGI
  • troponin complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001861552 – 211Troponin I, cardiac muscleAdd BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei5PhosphoserineBy similarity1
Modified residuei6PhosphoserineCombined sources1
Modified residuei23Phosphoserine; by PKA and PKD/PRKD1By similarity1
Modified residuei24Phosphoserine; by PKA and PKD/PRKD1By similarity1
Modified residuei27PhosphotyrosineBy similarity1
Modified residuei32Phosphothreonine; by STK4/MST1By similarity1
Modified residuei43Phosphoserine; by PKC/PRKCEBy similarity1
Modified residuei45Phosphoserine; by PKC/PRKCEBy similarity1
Modified residuei52Phosphothreonine; by STK4/MST1By similarity1
Modified residuei78PhosphoserineBy similarity1
Modified residuei79PhosphothreonineBy similarity1
Modified residuei130Phosphothreonine; by STK4/MST1By similarity1
Modified residuei144Phosphothreonine; by STK4/MST1By similarity1
Modified residuei151Phosphoserine; by PAK3By similarity1
Modified residuei167PhosphoserineBy similarity1
Modified residuei200PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-32. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). Phosphorylated at Ser-43 and Ser-45 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP23693.
PRIDEiP23693.

PTM databases

iPTMnetiP23693.
PhosphoSitePlusiP23693.

Expressioni

Gene expression databases

BgeeiENSRNOG00000018250.
GenevisibleiP23693. RN.

Interactioni

Subunit structurei

Interacts with TRIM63 (By similarity). Binds to actin and tropomyosin. Interacts with STK4/MST1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PDE4DIPQ5VU43-112EBI-10817583,EBI-10769071From a different organism.

Protein-protein interaction databases

BioGridi247923. 1 interactor.
IntActiP23693. 1 interactor.
STRINGi10116.ENSRNOP00000024640.

Structurei

3D structure databases

ProteinModelPortaliP23693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 80Involved in binding TNCAdd BLAST48
Regioni130 – 151Involved in binding TNC and actinAdd BLAST22

Sequence similaritiesi

Belongs to the troponin I family.Curated

Phylogenomic databases

eggNOGiKOG3977. Eukaryota.
ENOG410Y9IX. LUCA.
GeneTreeiENSGT00390000002746.
HOGENOMiHOG000293300.
HOVERGENiHBG052737.
InParanoidiP23693.
KOiK12044.
OMAiCRQLHTR.
OrthoDBiEOG091G0NOD.
PhylomeDBiP23693.
TreeFamiTF313374.

Family and domain databases

InterProiIPR001978. Troponin.
IPR021666. Troponin-I_N.
[Graphical view]
PfamiPF00992. Troponin. 1 hit.
PF11636. Troponin-I_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADESSDAAG EPQPAPAPVR RRSSANYRAY ATEPHAKKKS KISASRKLQL
60 70 80 90 100
KTLMLQIAKQ EMEREAEERR GEKGRVLSTR CQPLVLDGLG FEELQDLCRQ
110 120 130 140 150
LHARVDKVDE ERYDVEAKVT KNITEIADLT QKIYDLRGKF KRPTLRRVRI
160 170 180 190 200
SADAMMQALL GTRAKESLDL RAHLKQVKKE DIEKENREVG DWRKNIDALS
210
GMEGRKKKFE G
Length:211
Mass (Da):24,160
Last modified:January 23, 2007 - v2
Checksum:i17586B0FDB682B4C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8A → S in AAA42294 (PubMed:1886137).Curated1
Sequence conflicti182I → T in AAA42294 (PubMed:1886137).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57679 mRNA. Translation: AAA63504.1.
X58499 mRNA. Translation: CAA41402.1.
M92074 mRNA. Translation: AAA42294.1.
U77354 Genomic DNA. Translation: AAB52234.1.
DQ062462 mRNA. Translation: AAY63993.1.
PIRiA60124.
I56441.
RefSeqiNP_058840.1. NM_017144.2.
UniGeneiRn.64141.

Genome annotation databases

EnsembliENSRNOT00000024640; ENSRNOP00000024640; ENSRNOG00000018250.
GeneIDi29248.
KEGGirno:29248.
UCSCiRGD:62052. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57679 mRNA. Translation: AAA63504.1.
X58499 mRNA. Translation: CAA41402.1.
M92074 mRNA. Translation: AAA42294.1.
U77354 Genomic DNA. Translation: AAB52234.1.
DQ062462 mRNA. Translation: AAY63993.1.
PIRiA60124.
I56441.
RefSeqiNP_058840.1. NM_017144.2.
UniGeneiRn.64141.

3D structure databases

ProteinModelPortaliP23693.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247923. 1 interactor.
IntActiP23693. 1 interactor.
STRINGi10116.ENSRNOP00000024640.

PTM databases

iPTMnetiP23693.
PhosphoSitePlusiP23693.

Proteomic databases

PaxDbiP23693.
PRIDEiP23693.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024640; ENSRNOP00000024640; ENSRNOG00000018250.
GeneIDi29248.
KEGGirno:29248.
UCSCiRGD:62052. rat.

Organism-specific databases

CTDi7137.
RGDi62052. Tnni3.

Phylogenomic databases

eggNOGiKOG3977. Eukaryota.
ENOG410Y9IX. LUCA.
GeneTreeiENSGT00390000002746.
HOGENOMiHOG000293300.
HOVERGENiHBG052737.
InParanoidiP23693.
KOiK12044.
OMAiCRQLHTR.
OrthoDBiEOG091G0NOD.
PhylomeDBiP23693.
TreeFamiTF313374.

Enzyme and pathway databases

ReactomeiR-RNO-390522. Striated Muscle Contraction.
R-RNO-5578775. Ion homeostasis.

Miscellaneous databases

PROiP23693.

Gene expression databases

BgeeiENSRNOG00000018250.
GenevisibleiP23693. RN.

Family and domain databases

InterProiIPR001978. Troponin.
IPR021666. Troponin-I_N.
[Graphical view]
PfamiPF00992. Troponin. 1 hit.
PF11636. Troponin-I_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTNNI3_RAT
AccessioniPrimary (citable) accession number: P23693
Secondary accession number(s): Q4PP23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.