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P23687

- PPCE_PIG

UniProt

P23687 - PPCE_PIG

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Protein
Prolyl endopeptidase
Gene
PREP
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.

Catalytic activityi

Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei554 – 5541Charge relay system1 Publication
Active sitei641 – 6411Charge relay system
Active sitei680 – 6801Charge relay system1 Publication

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
  2. serine-type exopeptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS09.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prolyl endopeptidase (EC:3.4.21.26)
    Short name:
    PE
    Alternative name(s):
    Post-proline cleaving enzyme
    Gene namesi
    Name:PREP
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 710710Prolyl endopeptidase
    PRO_0000122403Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine By similarity
    Modified residuei157 – 1571N6-acetyllysine By similarity

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP23687.
    PRIDEiP23687.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Interactioni

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000004715.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi16 – 194
    Beta strandi22 – 254
    Helixi29 – 324
    Beta strandi34 – 363
    Helixi37 – 5620
    Helixi59 – 7113
    Beta strandi80 – 823
    Beta strandi85 – 917
    Beta strandi99 – 1057
    Beta strandi111 – 1144
    Helixi116 – 1194
    Beta strandi121 – 1233
    Beta strandi125 – 1328
    Beta strandi136 – 14510
    Beta strandi151 – 1577
    Turni158 – 1614
    Beta strandi162 – 17110
    Beta strandi176 – 1783
    Beta strandi182 – 1898
    Beta strandi198 – 2003
    Beta strandi209 – 2146
    Helixi219 – 2213
    Beta strandi223 – 2264
    Beta strandi235 – 2406
    Beta strandi246 – 2527
    Beta strandi254 – 2574
    Beta strandi260 – 2656
    Helixi266 – 2683
    Beta strandi269 – 2735
    Beta strandi280 – 2834
    Beta strandi285 – 2884
    Beta strandi290 – 2967
    Beta strandi299 – 3046
    Beta strandi312 – 3176
    Helixi323 – 3253
    Beta strandi327 – 3304
    Beta strandi337 – 3448
    Turni345 – 3473
    Beta strandi348 – 3558
    Beta strandi358 – 3658
    Turni366 – 3683
    Beta strandi371 – 3755
    Beta strandi379 – 3868
    Beta strandi392 – 3998
    Beta strandi401 – 4033
    Beta strandi406 – 4116
    Beta strandi414 – 4163
    Beta strandi420 – 4245
    Beta strandi428 – 4303
    Helixi432 – 4343
    Beta strandi435 – 4439
    Beta strandi445 – 4473
    Beta strandi449 – 4579
    Beta strandi468 – 4714
    Helixi486 – 49510
    Beta strandi498 – 5025
    Helixi511 – 5166
    Helixi520 – 5234
    Helixi524 – 53916
    Helixi545 – 5473
    Beta strandi548 – 5536
    Helixi555 – 56612
    Helixi568 – 5703
    Beta strandi572 – 5787
    Turni583 – 5853
    Helixi586 – 5883
    Helixi592 – 5954
    Helixi596 – 5994
    Helixi605 – 61410
    Helixi616 – 6183
    Beta strandi624 – 6274
    Beta strandi632 – 6387
    Beta strandi642 – 6443
    Helixi647 – 65913
    Turni660 – 6623
    Beta strandi663 – 6653
    Beta strandi670 – 6778
    Helixi686 – 70419

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1E5TX-ray1.70A1-710[»]
    1E8MX-ray1.50A1-710[»]
    1E8NX-ray1.50A1-710[»]
    1H2WX-ray1.39A1-710[»]
    1H2XX-ray1.49A1-710[»]
    1H2YX-ray1.78A1-710[»]
    1H2ZX-ray1.65A1-710[»]
    1O6FX-ray1.60A1-710[»]
    1O6GX-ray1.40A1-710[»]
    1QFMX-ray1.40A1-710[»]
    1QFSX-ray2.00A1-710[»]
    1UOOX-ray2.35A1-710[»]
    1UOPX-ray1.85A1-710[»]
    1UOQX-ray2.10A1-710[»]
    1VZ2X-ray2.20A1-710[»]
    1VZ3X-ray1.60A1-710[»]
    2XDWX-ray1.35A1-710[»]
    3EQ7X-ray2.89A1-710[»]
    3EQ8X-ray2.73A1-710[»]
    3EQ9X-ray2.47A1-710[»]
    4AMYX-ray2.00A1-710[»]
    4AMZX-ray2.00A1-710[»]
    4AN0X-ray2.20A1-710[»]
    4AN1X-ray1.90A1-710[»]
    4AX4X-ray1.60A1-710[»]
    4BCBX-ray1.70A1-710[»]
    4BCCX-ray1.65A1-710[»]
    4BCDX-ray1.50A1-710[»]
    ProteinModelPortaliP23687.
    SMRiP23687. Positions 1-710.

    Miscellaneous databases

    EvolutionaryTraceiP23687.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S9A family.

    Phylogenomic databases

    eggNOGiCOG1505.
    HOGENOMiHOG000238967.
    HOVERGENiHBG007251.
    KOiK01322.

    Family and domain databases

    Gene3Di2.130.10.120. 1 hit.
    3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR002471. Pept_S9_AS.
    IPR023302. Pept_S9A_N.
    IPR001375. Peptidase_S9.
    IPR002470. Peptidase_S9A.
    [Graphical view]
    PANTHERiPTHR11757. PTHR11757. 1 hit.
    PfamiPF00326. Peptidase_S9. 1 hit.
    PF02897. Peptidase_S9_N. 1 hit.
    [Graphical view]
    PRINTSiPR00862. PROLIGOPTASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23687-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSFQYPDVY RDETAIQDYH GHKVCDPYAW LEDPDSEQTK AFVEAQNKIT    50
    VPFLEQCPIR GLYKERMTEL YDYPKYSCHF KKGKRYFYFY NTGLQNQRVL 100
    YVQDSLEGEA RVFLDPNILS DDGTVALRGY AFSEDGEYFA YGLSASGSDW 150
    VTIKFMKVDG AKELPDVLER VKFSCMAWTH DGKGMFYNAY PQQDGKSDGT 200
    ETSTNLHQKL YYHVLGTDQS EDILCAEFPD EPKWMGGAEL SDDGRYVLLS 250
    IREGCDPVNR LWYCDLQQES NGITGILKWV KLIDNFEGEY DYVTNEGTVF 300
    TFKTNRHSPN YRLINIDFTD PEESKWKVLV PEHEKDVLEW VACVRSNFLV 350
    LCYLHDVKNT LQLHDLATGA LLKIFPLEVG SVVGYSGQKK DTEIFYQFTS 400
    FLSPGIIYHC DLTKEELEPR VFREVTVKGI DASDYQTVQI FYPSKDGTKI 450
    PMFIVHKKGI KLDGSHPAFL YGYGGFNISI TPNYSVSRLI FVRHMGGVLA 500
    VANIRGGGEY GETWHKGGIL ANKQNCFDDF QCAAEYLIKE GYTSPKRLTI 550
    NGGSNGGLLV ATCANQRPDL FGCVIAQVGV MDMLKFHKYT IGHAWTTDYG 600
    CSDSKQHFEW LIKYSPLHNV KLPEADDIQY PSMLLLTADH DDRVVPLHSL 650
    KFIATLQYIV GRSRKQNNPL LIHVDTKAGH GAGKPTAKVI EEVSDMFAFI 700
    ARCLNIDWIP 710
    Length:710
    Mass (Da):80,770
    Last modified:November 1, 1991 - v1
    Checksum:i70286A86238D72C0
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti29 – 291A → H.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64227 mRNA. Translation: AAA31110.1.
    PIRiA37942.
    RefSeqiNP_001004050.1. NM_001004050.1.
    UniGeneiSsc.10442.

    Genome annotation databases

    GeneIDi445540.
    KEGGissc:445540.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64227 mRNA. Translation: AAA31110.1 .
    PIRi A37942.
    RefSeqi NP_001004050.1. NM_001004050.1.
    UniGenei Ssc.10442.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1E5T X-ray 1.70 A 1-710 [» ]
    1E8M X-ray 1.50 A 1-710 [» ]
    1E8N X-ray 1.50 A 1-710 [» ]
    1H2W X-ray 1.39 A 1-710 [» ]
    1H2X X-ray 1.49 A 1-710 [» ]
    1H2Y X-ray 1.78 A 1-710 [» ]
    1H2Z X-ray 1.65 A 1-710 [» ]
    1O6F X-ray 1.60 A 1-710 [» ]
    1O6G X-ray 1.40 A 1-710 [» ]
    1QFM X-ray 1.40 A 1-710 [» ]
    1QFS X-ray 2.00 A 1-710 [» ]
    1UOO X-ray 2.35 A 1-710 [» ]
    1UOP X-ray 1.85 A 1-710 [» ]
    1UOQ X-ray 2.10 A 1-710 [» ]
    1VZ2 X-ray 2.20 A 1-710 [» ]
    1VZ3 X-ray 1.60 A 1-710 [» ]
    2XDW X-ray 1.35 A 1-710 [» ]
    3EQ7 X-ray 2.89 A 1-710 [» ]
    3EQ8 X-ray 2.73 A 1-710 [» ]
    3EQ9 X-ray 2.47 A 1-710 [» ]
    4AMY X-ray 2.00 A 1-710 [» ]
    4AMZ X-ray 2.00 A 1-710 [» ]
    4AN0 X-ray 2.20 A 1-710 [» ]
    4AN1 X-ray 1.90 A 1-710 [» ]
    4AX4 X-ray 1.60 A 1-710 [» ]
    4BCB X-ray 1.70 A 1-710 [» ]
    4BCC X-ray 1.65 A 1-710 [» ]
    4BCD X-ray 1.50 A 1-710 [» ]
    ProteinModelPortali P23687.
    SMRi P23687. Positions 1-710.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000004715.

    Chemistry

    BindingDBi P23687.
    ChEMBLi CHEMBL2461.

    Protein family/group databases

    MEROPSi S09.001.

    Proteomic databases

    PaxDbi P23687.
    PRIDEi P23687.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 445540.
    KEGGi ssc:445540.

    Organism-specific databases

    CTDi 5550.

    Phylogenomic databases

    eggNOGi COG1505.
    HOGENOMi HOG000238967.
    HOVERGENi HBG007251.
    KOi K01322.

    Miscellaneous databases

    EvolutionaryTracei P23687.

    Family and domain databases

    Gene3Di 2.130.10.120. 1 hit.
    3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR002471. Pept_S9_AS.
    IPR023302. Pept_S9A_N.
    IPR001375. Peptidase_S9.
    IPR002470. Peptidase_S9A.
    [Graphical view ]
    PANTHERi PTHR11757. PTHR11757. 1 hit.
    Pfami PF00326. Peptidase_S9. 1 hit.
    PF02897. Peptidase_S9_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00862. PROLIGOPTASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00708. PRO_ENDOPEP_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning of porcine brain prolyl endopeptidase and identification of the active-site seryl residue."
      Rennex D., Hemmings B.A., Hofsteenge J., Stone S.R.
      Biochemistry 30:2195-2203(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, ACTIVE SITE SER-554.
      Tissue: Brain.
    2. "Inactivation of prolyl endopeptidase by a peptidylchloromethane. Kinetics of inactivation and identification of sites of modification."
      Stone S.R., Rennex D., Wikstrom P., Shaw E., Hofsteenge J.
      Biochem. J. 276:837-840(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVE SITE HIS-680.
    3. "Prolyl oligopeptidase: an unusual beta-propeller domain regulates proteolysis."
      Fueloep V., Bocskei Z., Polgar L.
      Cell 94:161-170(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS).

    Entry informationi

    Entry nameiPPCE_PIG
    AccessioniPrimary (citable) accession number: P23687
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: June 11, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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