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Protein

Butyrate--acetoacetate CoA-transferase subunit B

Gene

ctfB

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation.

Catalytic activityi

Butanoyl-CoA + acetoacetate = butanoate + acetoacetyl-CoA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei51 – 511PROSITE-ProRule annotation

GO - Molecular functioni

  1. butyrate-acetoacetate CoA-transferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciCACE272562:GJIH-4009-MONOMER.
MetaCyc:COATBCLOS-MONOMER.
SABIO-RKP23673.

Names & Taxonomyi

Protein namesi
Recommended name:
Butyrate--acetoacetate CoA-transferase subunit B (EC:2.8.3.9)
Short name:
Coat B
Alternative name(s):
Acetoacetyl-CoA:acetate/butyrate CoA-transferase subunit B
Gene namesi
Name:ctfB
Ordered Locus Names:CA_P0164
Encoded oniPlasmid pSOL10 Publication
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000814 Componenti: Plasmid pSOL1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 221221Butyrate--acetoacetate CoA-transferase subunit BPRO_0000157926Add
BLAST

Expressioni

Inductioni

Induced during switch to solvent production.

Interactioni

Subunit structurei

Heterodimer.

Protein-protein interaction databases

STRINGi272562.CA_P0164.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2057.
HOGENOMiHOG000221245.
KOiK01035.
OMAiTIDADMI.
OrthoDBiEOG6HMXJG.

Family and domain databases

InterProiIPR012791. 3-oxoacid_CoA-transf_B.
IPR004165. CoA_trans_fam_I.
IPR004164. CoA_transf_AS.
[Graphical view]
PANTHERiPTHR13707. PTHR13707. 1 hit.
PfamiPF01144. CoA_trans. 1 hit.
[Graphical view]
SMARTiSM00882. CoA_trans. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITEiPS01274. COA_TRANSF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23673-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINDKNLAKE IIAKRVAREL KNGQLVNLGV GLPTMVADYI PKNFKITFQS
60 70 80 90 100
ENGIVGMGAS PKINEADKDV VNAGGDYTTV LPDGTFFDSS VSFSLIRGGH
110 120 130 140 150
VDVTVLGALQ VDEKGNIANW IVPGKMLSGM GGAMDLVNGA KKVIIAMRHT
160 170 180 190 200
NKGQPKILKK CTLPLTAKSQ ANLIVTELGV IEVINDGLLL TEINKNTTID
210 220
EIRSLTAADL LISNELRPMA V
Length:221
Mass (Da):23,624
Last modified:November 1, 1991 - v1
Checksum:i9F9920709628F800
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55392 Genomic DNA. Translation: AAA63762.1.
X72831 Genomic DNA. Translation: CAA51346.1.
M93363 Genomic DNA. Translation: AAB53233.1.
AE001438 Genomic DNA. Translation: AAK76909.1.
PIRiJN0489.
RefSeqiNP_149327.1. NC_001988.2.
WP_010890848.1. NC_001988.2.

Genome annotation databases

EnsemblBacteriaiAAK76909; AAK76909; CA_P0164.
GeneIDi1116169.
KEGGicac:CA_P0164.
PATRICi32034214. VBICloAce74127_0165.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55392 Genomic DNA. Translation: AAA63762.1.
X72831 Genomic DNA. Translation: CAA51346.1.
M93363 Genomic DNA. Translation: AAB53233.1.
AE001438 Genomic DNA. Translation: AAK76909.1.
PIRiJN0489.
RefSeqiNP_149327.1. NC_001988.2.
WP_010890848.1. NC_001988.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272562.CA_P0164.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK76909; AAK76909; CA_P0164.
GeneIDi1116169.
KEGGicac:CA_P0164.
PATRICi32034214. VBICloAce74127_0165.

Phylogenomic databases

eggNOGiCOG2057.
HOGENOMiHOG000221245.
KOiK01035.
OMAiTIDADMI.
OrthoDBiEOG6HMXJG.

Enzyme and pathway databases

BioCyciCACE272562:GJIH-4009-MONOMER.
MetaCyc:COATBCLOS-MONOMER.
SABIO-RKP23673.

Family and domain databases

InterProiIPR012791. 3-oxoacid_CoA-transf_B.
IPR004165. CoA_trans_fam_I.
IPR004164. CoA_transf_AS.
[Graphical view]
PANTHERiPTHR13707. PTHR13707. 1 hit.
PfamiPF01144. CoA_trans. 1 hit.
[Graphical view]
SMARTiSM00882. CoA_trans. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITEiPS01274. COA_TRANSF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum."
    Gerischer U., Duerre P.
    J. Bacteriol. 172:6907-6918(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  2. "Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis."
    Fischer R.J., Helms J., Duerre P.
    J. Bacteriol. 175:6959-6969(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  3. "Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824."
    Petersen D.J., Cary J.W., Vanderleyden J., Bennett G.N.
    Gene 123:93-97(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19.
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  5. "Cloning and expression of Clostridium acetobutylicum ATCC 824 acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase in Escherichia coli."
    Cary J.W., Petersen D.J., Papoutsakis E.T., Bennett G.N.
    Appl. Environ. Microbiol. 56:1576-1583(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-8.
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiCTFB_CLOAB
AccessioniPrimary (citable) accession number: P23673
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 1, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.