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Protein

Alpha-amylase

Gene

amyA

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi143 – 1431CalciumBy similarity
Metal bindingi184 – 1841Calcium; via carbonyl oxygenBy similarity
Metal bindingi192 – 1921CalciumBy similarity
Active sitei222 – 2221NucleophileBy similarity
Metal bindingi226 – 2261Calcium; via carbonyl oxygenBy similarity
Active sitei262 – 2621Proton donorBy similarity
Sitei321 – 3211Transition state stabilizerBy similarity

GO - Molecular functioni

  1. alpha-amylase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. starch binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciCACE272562:GJIH-4013-MONOMER.

Protein family/group databases

CAZyiCBM25. Carbohydrate-Binding Module Family 25.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:amyA
Synonyms:amyP
Ordered Locus Names:CA_P0168
Encoded oniPlasmid pSOL10 Publication
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000814: Plasmid pSOL1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Sequence AnalysisAdd
BLAST
Chaini35 – 760726Alpha-amylasePRO_0000001338Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi272562.CA_P0168.

Structurei

3D structure databases

ProteinModelPortaliP23671.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0366.
HOGENOMiHOG000292529.
KOiK01176.
OMAiGQNIFVV.
OrthoDBiEOG6F55CH.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR005085. CBM_fam25.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF03423. CBM_25. 3 hits.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00632. Aamy_C. 1 hit.
SM01066. CBM_25. 3 hits.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23671-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRSKLLKR RMLSLSVICV LIGYGPVFNP VRSQAKVMTY SSENRELPEN
60 70 80 90 100
TKDGVMLHAF DWSFNNIKKE LPSIAAAGYK AVQVSPVQGT KSNSTNSSDW
110 120 130 140 150
WLLYQPTNQA IGNAQLGSYD DFKSLCSEAK NYGISIVVDV VMNHMANNGN
160 170 180 190 200
DDEVASEVDP SFKDPSLYHH NGQCTDWNNR QDVTQEGIGM PDLNTQSSAV
210 220 230 240 250
QSKAITFLNQ CVDAGATGFR FDAAKHIETD LGLDANKSWS GNYWENVLGS
260 270 280 290 300
LHNKSNLYIY GEVLQDGKVD NISAYESFMN VEASAYDGSL RGAIKSGDLT
310 320 330 340 350
NAQGMGGLDS NKCVDMLETH DEYEHNESKD LTDWQRKAGW AIAASRAGSV
360 370 380 390 400
PLFFDRPTGN IGSEGDALWK DSDVVAVNEF HNAMAGQNEY LRLQNNNKAM
410 420 430 440 450
IIERGSKGAV IVNEGDSFNL NTPTNLEDGN YDNHGSATDS LTVSQGRMTG
460 470 480 490 500
TVPANSIIVI YNKNSNPGSD RVTLSEQAAK AGDSVTITYD AGTTALKDAS
510 520 530 540 550
NVNLYWGYDG FSAATSKAMT SLGDNKWQTT ITVPKEVTKN VNFSFTDGTS
560 570 580 590 600
WDNNNGANWN IPLASNYLPH AGYKVDYDSS NLVSGNNFTI YYNGNLANSS
610 620 630 640 650
NVSLHWGVNG WSNMQNLAMV KDSNGFWEAT IAIPASSNTL NFCFTNGSSW
660 670 680 690 700
DNNNNNNWTL NTWSSVPKVQ VTPAPEACKQ ISVYYNGSLA SSASNITLHW
710 720 730 740 750
GCNGFTSPQD INMVKQADGR WLANITLPSG CYNVNMAFKD QSGTWDNNNS
760
NNYNFSSTNN
Length:760
Mass (Da):83,045
Last modified:August 29, 2001 - v2
Checksum:i10E146F40252F6D0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti213 – 2131D → H in AAA63759 (Ref. 1) Curated
Sequence conflicti222 – 2221D → H in AAA63759 (Ref. 1) Curated
Sequence conflicti571 – 5711A → G in AAA63759 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55392 Genomic DNA. Translation: AAA63759.2.
AF164199 Genomic DNA. Translation: AAD47072.1.
AE001438 Genomic DNA. Translation: AAK76913.1.
PIRiB37837.
RefSeqiNP_149331.1. NC_001988.2.
YP_009075958.1. NG_035184.1.

Genome annotation databases

EnsemblBacteriaiAAK76913; AAK76913; CA_P0168.
GeneIDi1116173.
KEGGicac:CA_P0168.
PATRICi32034222. VBICloAce74127_0169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55392 Genomic DNA. Translation: AAA63759.2.
AF164199 Genomic DNA. Translation: AAD47072.1.
AE001438 Genomic DNA. Translation: AAK76913.1.
PIRiB37837.
RefSeqiNP_149331.1. NC_001988.2.
YP_009075958.1. NG_035184.1.

3D structure databases

ProteinModelPortaliP23671.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272562.CA_P0168.

Protein family/group databases

CAZyiCBM25. Carbohydrate-Binding Module Family 25.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK76913; AAK76913; CA_P0168.
GeneIDi1116173.
KEGGicac:CA_P0168.
PATRICi32034222. VBICloAce74127_0169.

Phylogenomic databases

eggNOGiCOG0366.
HOGENOMiHOG000292529.
KOiK01176.
OMAiGQNIFVV.
OrthoDBiEOG6F55CH.

Enzyme and pathway databases

BioCyciCACE272562:GJIH-4013-MONOMER.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR005085. CBM_fam25.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF03423. CBM_25. 3 hits.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00632. Aamy_C. 1 hit.
SM01066. CBM_25. 3 hits.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis and ECF sigma factor-dependent expression of an alpha-amylase gene from Clostridium acetobutylicum DSM 792."
    Schaffer S., Duerre P.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  2. "Molecular characterization of amyP, a pSOL1 located gene coding the major alpha-amylase of Clostridium acetobutylicum ATCC824, and its use as a reporter system for strain degeneration."
    Sabathe F., Cornillot E., Croux C., Soucaille P.
    Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  4. "Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum."
    Gerischer U., Duerre P.
    J. Bacteriol. 172:6907-6918(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 292-760.
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiAMY_CLOAB
AccessioniPrimary (citable) accession number: P23671
Secondary accession number(s): Q9S429
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: August 29, 2001
Last modified: January 7, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.