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P23671

- AMY_CLOAB

UniProt

P23671 - AMY_CLOAB

Protein

Alpha-amylase

Gene

amyA

Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (29 Aug 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi143 – 1431CalciumBy similarity
    Metal bindingi184 – 1841Calcium; via carbonyl oxygenBy similarity
    Metal bindingi192 – 1921CalciumBy similarity
    Active sitei222 – 2221NucleophileBy similarity
    Metal bindingi226 – 2261Calcium; via carbonyl oxygenBy similarity
    Active sitei262 – 2621Proton donorBy similarity
    Sitei321 – 3211Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. alpha-amylase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. starch binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BioCyciCACE272562:GJIH-4013-MONOMER.

    Protein family/group databases

    CAZyiCBM25. Carbohydrate-Binding Module Family 25.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-amylase (EC:3.2.1.1)
    Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase
    Gene namesi
    Name:amyA
    Synonyms:amyP
    Ordered Locus Names:CA_P0168
    Encoded oniPlasmid pSOL10 Publication
    OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
    Taxonomic identifieri272562 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000814: Plasmid pSOL1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3434Sequence AnalysisAdd
    BLAST
    Chaini35 – 760726Alpha-amylasePRO_0000001338Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi272562.CA_P0168.

    Structurei

    3D structure databases

    ProteinModelPortaliP23671.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0366.
    HOGENOMiHOG000292529.
    KOiK01176.
    OMAiGQNIFVV.
    OrthoDBiEOG6F55CH.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR006048. A-amylase_b_C.
    IPR006046. Alpha_amylase.
    IPR005085. CBM_fam25.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF03423. CBM_25. 3 hits.
    [Graphical view]
    PRINTSiPR00110. ALPHAAMYLASE.
    SMARTiSM00632. Aamy_C. 1 hit.
    SM01066. CBM_25. 3 hits.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23671-1 [UniParc]FASTAAdd to Basket

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    MSKRSKLLKR RMLSLSVICV LIGYGPVFNP VRSQAKVMTY SSENRELPEN    50
    TKDGVMLHAF DWSFNNIKKE LPSIAAAGYK AVQVSPVQGT KSNSTNSSDW 100
    WLLYQPTNQA IGNAQLGSYD DFKSLCSEAK NYGISIVVDV VMNHMANNGN 150
    DDEVASEVDP SFKDPSLYHH NGQCTDWNNR QDVTQEGIGM PDLNTQSSAV 200
    QSKAITFLNQ CVDAGATGFR FDAAKHIETD LGLDANKSWS GNYWENVLGS 250
    LHNKSNLYIY GEVLQDGKVD NISAYESFMN VEASAYDGSL RGAIKSGDLT 300
    NAQGMGGLDS NKCVDMLETH DEYEHNESKD LTDWQRKAGW AIAASRAGSV 350
    PLFFDRPTGN IGSEGDALWK DSDVVAVNEF HNAMAGQNEY LRLQNNNKAM 400
    IIERGSKGAV IVNEGDSFNL NTPTNLEDGN YDNHGSATDS LTVSQGRMTG 450
    TVPANSIIVI YNKNSNPGSD RVTLSEQAAK AGDSVTITYD AGTTALKDAS 500
    NVNLYWGYDG FSAATSKAMT SLGDNKWQTT ITVPKEVTKN VNFSFTDGTS 550
    WDNNNGANWN IPLASNYLPH AGYKVDYDSS NLVSGNNFTI YYNGNLANSS 600
    NVSLHWGVNG WSNMQNLAMV KDSNGFWEAT IAIPASSNTL NFCFTNGSSW 650
    DNNNNNNWTL NTWSSVPKVQ VTPAPEACKQ ISVYYNGSLA SSASNITLHW 700
    GCNGFTSPQD INMVKQADGR WLANITLPSG CYNVNMAFKD QSGTWDNNNS 750
    NNYNFSSTNN 760
    Length:760
    Mass (Da):83,045
    Last modified:August 29, 2001 - v2
    Checksum:i10E146F40252F6D0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti213 – 2131D → H in AAA63759. 1 PublicationCurated
    Sequence conflicti222 – 2221D → H in AAA63759. 1 PublicationCurated
    Sequence conflicti571 – 5711A → G in AAA63759. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55392 Genomic DNA. Translation: AAA63759.2.
    AF164199 Genomic DNA. Translation: AAD47072.1.
    AE001438 Genomic DNA. Translation: AAK76913.1.
    PIRiB37837.
    RefSeqiNP_149331.1. NC_001988.2.

    Genome annotation databases

    EnsemblBacteriaiAAK76913; AAK76913; CA_P0168.
    GeneIDi1116173.
    KEGGicac:CA_P0168.
    PATRICi32034222. VBICloAce74127_0169.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55392 Genomic DNA. Translation: AAA63759.2 .
    AF164199 Genomic DNA. Translation: AAD47072.1 .
    AE001438 Genomic DNA. Translation: AAK76913.1 .
    PIRi B37837.
    RefSeqi NP_149331.1. NC_001988.2.

    3D structure databases

    ProteinModelPortali P23671.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272562.CA_P0168.

    Protein family/group databases

    CAZyi CBM25. Carbohydrate-Binding Module Family 25.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK76913 ; AAK76913 ; CA_P0168 .
    GeneIDi 1116173.
    KEGGi cac:CA_P0168.
    PATRICi 32034222. VBICloAce74127_0169.

    Phylogenomic databases

    eggNOGi COG0366.
    HOGENOMi HOG000292529.
    KOi K01176.
    OMAi GQNIFVV.
    OrthoDBi EOG6F55CH.

    Enzyme and pathway databases

    BioCyci CACE272562:GJIH-4013-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR006048. A-amylase_b_C.
    IPR006046. Alpha_amylase.
    IPR005085. CBM_fam25.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF03423. CBM_25. 3 hits.
    [Graphical view ]
    PRINTSi PR00110. ALPHAAMYLASE.
    SMARTi SM00632. Aamy_C. 1 hit.
    SM01066. CBM_25. 3 hits.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence analysis and ECF sigma factor-dependent expression of an alpha-amylase gene from Clostridium acetobutylicum DSM 792."
      Schaffer S., Duerre P.
      Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
    2. "Molecular characterization of amyP, a pSOL1 located gene coding the major alpha-amylase of Clostridium acetobutylicum ATCC824, and its use as a reporter system for strain degeneration."
      Sabathe F., Cornillot E., Croux C., Soucaille P.
      Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
    4. "Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum."
      Gerischer U., Duerre P.
      J. Bacteriol. 172:6907-6918(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 292-760.
      Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

    Entry informationi

    Entry nameiAMY_CLOAB
    AccessioniPrimary (citable) accession number: P23671
    Secondary accession number(s): Q9S429
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: August 29, 2001
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3