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P23671

- AMY_CLOAB

UniProt

P23671 - AMY_CLOAB

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Protein
Alpha-amylase
Gene
amyA, amyP, CA_P0168
Organism
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Binds 1 calcium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi143 – 1431Calcium By similarity
Metal bindingi184 – 1841Calcium; via carbonyl oxygen By similarity
Metal bindingi192 – 1921Calcium By similarity
Active sitei222 – 2221Nucleophile By similarity
Metal bindingi226 – 2261Calcium; via carbonyl oxygen By similarity
Active sitei262 – 2621Proton donor By similarity
Sitei321 – 3211Transition state stabilizer By similarity

GO - Molecular functioni

  1. alpha-amylase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. starch binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciCACE272562:GJIH-4013-MONOMER.

Protein family/group databases

CAZyiCBM25. Carbohydrate-Binding Module Family 25.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:amyA
Synonyms:amyP
Ordered Locus Names:CA_P0168
Encoded oniPlasmid pSOL10 Publication
OrganismiClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Taxonomic identifieri272562 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000814: Plasmid pSOL1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434 Reviewed prediction
Add
BLAST
Chaini35 – 760726Alpha-amylase
PRO_0000001338Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

STRINGi272562.CA_P0168.

Structurei

3D structure databases

ProteinModelPortaliP23671.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0366.
HOGENOMiHOG000292529.
KOiK01176.
OMAiGQNIFVV.
OrthoDBiEOG6F55CH.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR005085. CBM_fam25.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF03423. CBM_25. 3 hits.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00632. Aamy_C. 1 hit.
SM01066. CBM_25. 3 hits.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23671-1 [UniParc]FASTAAdd to Basket

« Hide

MSKRSKLLKR RMLSLSVICV LIGYGPVFNP VRSQAKVMTY SSENRELPEN    50
TKDGVMLHAF DWSFNNIKKE LPSIAAAGYK AVQVSPVQGT KSNSTNSSDW 100
WLLYQPTNQA IGNAQLGSYD DFKSLCSEAK NYGISIVVDV VMNHMANNGN 150
DDEVASEVDP SFKDPSLYHH NGQCTDWNNR QDVTQEGIGM PDLNTQSSAV 200
QSKAITFLNQ CVDAGATGFR FDAAKHIETD LGLDANKSWS GNYWENVLGS 250
LHNKSNLYIY GEVLQDGKVD NISAYESFMN VEASAYDGSL RGAIKSGDLT 300
NAQGMGGLDS NKCVDMLETH DEYEHNESKD LTDWQRKAGW AIAASRAGSV 350
PLFFDRPTGN IGSEGDALWK DSDVVAVNEF HNAMAGQNEY LRLQNNNKAM 400
IIERGSKGAV IVNEGDSFNL NTPTNLEDGN YDNHGSATDS LTVSQGRMTG 450
TVPANSIIVI YNKNSNPGSD RVTLSEQAAK AGDSVTITYD AGTTALKDAS 500
NVNLYWGYDG FSAATSKAMT SLGDNKWQTT ITVPKEVTKN VNFSFTDGTS 550
WDNNNGANWN IPLASNYLPH AGYKVDYDSS NLVSGNNFTI YYNGNLANSS 600
NVSLHWGVNG WSNMQNLAMV KDSNGFWEAT IAIPASSNTL NFCFTNGSSW 650
DNNNNNNWTL NTWSSVPKVQ VTPAPEACKQ ISVYYNGSLA SSASNITLHW 700
GCNGFTSPQD INMVKQADGR WLANITLPSG CYNVNMAFKD QSGTWDNNNS 750
NNYNFSSTNN 760
Length:760
Mass (Da):83,045
Last modified:August 29, 2001 - v2
Checksum:i10E146F40252F6D0
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti213 – 2131D → H in AAA63759. 1 Publication
Sequence conflicti222 – 2221D → H in AAA63759. 1 Publication
Sequence conflicti571 – 5711A → G in AAA63759. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M55392 Genomic DNA. Translation: AAA63759.2.
AF164199 Genomic DNA. Translation: AAD47072.1.
AE001438 Genomic DNA. Translation: AAK76913.1.
PIRiB37837.
RefSeqiNP_149331.1. NC_001988.2.

Genome annotation databases

EnsemblBacteriaiAAK76913; AAK76913; CA_P0168.
GeneIDi1116173.
KEGGicac:CA_P0168.
PATRICi32034222. VBICloAce74127_0169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M55392 Genomic DNA. Translation: AAA63759.2 .
AF164199 Genomic DNA. Translation: AAD47072.1 .
AE001438 Genomic DNA. Translation: AAK76913.1 .
PIRi B37837.
RefSeqi NP_149331.1. NC_001988.2.

3D structure databases

ProteinModelPortali P23671.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272562.CA_P0168.

Protein family/group databases

CAZyi CBM25. Carbohydrate-Binding Module Family 25.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK76913 ; AAK76913 ; CA_P0168 .
GeneIDi 1116173.
KEGGi cac:CA_P0168.
PATRICi 32034222. VBICloAce74127_0169.

Phylogenomic databases

eggNOGi COG0366.
HOGENOMi HOG000292529.
KOi K01176.
OMAi GQNIFVV.
OrthoDBi EOG6F55CH.

Enzyme and pathway databases

BioCyci CACE272562:GJIH-4013-MONOMER.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR006046. Alpha_amylase.
IPR005085. CBM_fam25.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF03423. CBM_25. 3 hits.
[Graphical view ]
PRINTSi PR00110. ALPHAAMYLASE.
SMARTi SM00632. Aamy_C. 1 hit.
SM01066. CBM_25. 3 hits.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis and ECF sigma factor-dependent expression of an alpha-amylase gene from Clostridium acetobutylicum DSM 792."
    Schaffer S., Duerre P.
    Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  2. "Molecular characterization of amyP, a pSOL1 located gene coding the major alpha-amylase of Clostridium acetobutylicum ATCC824, and its use as a reporter system for strain degeneration."
    Sabathe F., Cornillot E., Croux C., Soucaille P.
    Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
  4. "Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum."
    Gerischer U., Duerre P.
    J. Bacteriol. 172:6907-6918(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 292-760.
    Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.

Entry informationi

Entry nameiAMY_CLOAB
AccessioniPrimary (citable) accession number: P23671
Secondary accession number(s): Q9S429
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: August 29, 2001
Last modified: May 14, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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