Reviewed, UniProtKB/Swiss-Prot P23666 (GUN2_PERAE)

Names and origin

Protein names

Recommended name:
    Endoglucanase 2
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta-glucanase 2
    Abscission cellulase 2

Gene names

Name:

CEL2

Organism

Persea americana (Avocado)

Taxonomic identifier

3435 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › magnoliids › Laurales › Lauraceae › Persea

Protein attributes

Sequence length	130 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in ripening fruit process.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Developmental stage	In ripening fruit.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Fruit ripening Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW ripening Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 130	›130	Endoglucanase 2		PRO_0000184059
Sites
	Active site	47	1	By similarity
	Active site	98	1	By similarity
	Active site	107	1	By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P23666-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: A8A9EBC7B0B6F5B6	FASTA	130	13,892
	10 20 30 40 50 60 ASCGSTTVTA KNLISLAKKQ VDYILGENPA KMSYMVGFGE RYPQHVHHRG SSLPSVHAHP 70 80 90 100 110 120 NPIPCNAGFQ YLYSSSPNPN ILVGAILGGP DSRDSFSDDR NNYQQSEPAT YINAPLVGAL 130 AFFAANPVAN

References

[1]	"Isolation and characterization of a cellulase gene family member expressed during avocado fruit ripening." Cass L.G., Kirven K.A., Christoffersen R.E. Mol. Gen. Genet. 223:76-86(1990) [PubMed: 2259345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Hass. Tissue: Fruit.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55790 Genomic DNA. Translation: CAA39314.1.
PIR	S34493.
3D structure databases
HSSP	HSSP built from PDB template 1IA6 based on UniProtKB Q9EYQ2.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Enzyme and pathway databases
BRENDA	3.2.1.4. 2559.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN2_PERAE

Accession

Primary (citable) accession number: P23666

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	May 5, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)