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P23665

- GUNA_FIBSU

UniProt

P23665 - GUNA_FIBSU

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Protein

Endoglucanase A

Gene
endA
Organism
Fibrobacter succinogenes (Bacteroides succinogenes)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

High levels of endoglucanase activity detected on acid-swollen cellulose, ball-milled cellulose, and carboxymethyl cellulose; moderate levels detected on filter paper, phosphoric acid-swollen cellulose, lichenan, and xylan.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 5.0.

Temperature dependencei

Optimum temperature is 37-39 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei417 – 4171 By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase A (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:endA
OrganismiFibrobacter succinogenes (Bacteroides succinogenes)
Taxonomic identifieri833 [NCBI]
Taxonomic lineageiBacteriaFibrobacteresFibrobacteralesFibrobacteraceaeFibrobacter

Subcellular locationi

Periplasm
Note: 80% periplasmic, 16% extracellular upon overexpression in E.coli.1 Publication

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626 Reviewed predictionAdd
BLAST
Chaini27 – 453427Endoglucanase APRO_0000007954Add
BLAST

Interactioni

Subunit structurei

Monomer Inferred.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP23665.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni115 – 12612Linker ("hinge") (Pro-Thr box)Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23665-1 [UniParc]FASTAAdd to Basket

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MNCRKYLLSG LAVFGLAATS AVAALSTDDY VEAAWMTTRF FGAQRSGQGP    50
NWILDGTSNP TSFTKDSYNG KDVSGGWFDC GDHVMYGQSQ GYASYVLALA 100
YAEFTEVSTT FILVTTPTTR KPTTTPMKSG KPNKVRDLLE ELRYEADFWV 150
KAAIDGNNFV TVKGDGNADH QKWVTAGAMS KLGSGEGGEP RCITGNANDG 200
FTSGLAAAML AVMARVDPDT ANQAKYLKAA KTAYSYAKSH KGVTNSQGFY 250
ESSWWDGRWE DGPFLAELEL YRTTGENSYK TAAIDRYDNL KFSLGEGTHF 300
MYSNVVPLSA VMAEAVFEET PHGMRKEAIG VLDLIYEEKA KDKIFQNPNG 350
MGSGKFPVRV PSGGAFLYAL SDKFNNTNEH MEMIEKNVSY LLGDNGSKKS 400
YVVGFSKNGA NAPSRPHHRG YYANEKRWRR SRRCSESSRK EQALGRYDCW 450
RLY 453
Length:453
Mass (Da):50,042
Last modified:November 1, 1991 - v1
Checksum:i7E861B76070D1186
GO

Sequence cautioni

The sequence AAA24895.1 differs from that shown. Reason: Frameshift at position 127.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58520 Genomic DNA. Translation: AAA24894.1.
M58520 Genomic DNA. Translation: AAA24895.1. Frameshift.
PIRiA39416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58520 Genomic DNA. Translation: AAA24894.1 .
M58520 Genomic DNA. Translation: AAA24895.1 . Frameshift.
PIRi A39416.

3D structure databases

ProteinModelPortali P23665.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view ]
Pfami PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "endAFS, a novel family E endoglucanase gene from Fibrobacter succinogenes AR1."
    Cavicchioli R., East P.D., Watson K.
    J. Bacteriol. 173:3265-3268(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AR1.
  2. "The involvement of transcriptional read-through from internal promoters in the expression of a novel endoglucanase gene FSendA, from Fibrobacter succinogenes AR1."
    Cavicchioli R., Watson K.
    Nucleic Acids Res. 19:1661-1669(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: RIBOSOMAL FRAMESHIFT.
    Strain: AR1.
  3. "Molecular cloning, expression, and characterization of endoglucanase genes from Fibrobacter succinogenes AR1."
    Cavicchioli R., Watson K.
    Appl. Environ. Microbiol. 57:359-365(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBSTRATES, SUBUNIT, SUBCELLULAR LOCATION, CHARACTERIZATION.
    Strain: AR1.

Entry informationi

Entry nameiGUNA_FIBSU
AccessioniPrimary (citable) accession number: P23665
Secondary accession number(s): P23664
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Expression of endA requires a ribosomal frameshift to allow contiguous translation of a 453 amino acid protein; this is suggested to take place at position 127 but could in fact occur anywhere between positions 82 and 127.
On the basis of sequence similarities the C-terminal part of this sequence seems to be incorrect.

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3