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P23665

- GUNA_FIBSU

UniProt

P23665 - GUNA_FIBSU

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Protein

Endoglucanase A

Gene

endA

Organism
Fibrobacter succinogenes (Bacteroides succinogenes)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

High levels of endoglucanase activity detected on acid-swollen cellulose, ball-milled cellulose, and carboxymethyl cellulose; moderate levels detected on filter paper, phosphoric acid-swollen cellulose, lichenan, and xylan.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

pH dependencei

Optimum pH is 5.0.

Temperature dependencei

Optimum temperature is 37-39 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei417 – 4171PROSITE-ProRule annotation

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase A (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Gene namesi
Name:endA
OrganismiFibrobacter succinogenes (Bacteroides succinogenes)
Taxonomic identifieri833 [NCBI]
Taxonomic lineageiBacteriaFibrobacteresFibrobacteralesFibrobacteraceaeFibrobacter

Subcellular locationi

Periplasm 1 Publication
Note: 80% periplasmic, 16% extracellular upon overexpression in E.coli.

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 453427Endoglucanase APRO_0000007954Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP23665.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni115 – 12612Linker ("hinge") (Pro-Thr box)Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23665-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNCRKYLLSG LAVFGLAATS AVAALSTDDY VEAAWMTTRF FGAQRSGQGP
60 70 80 90 100
NWILDGTSNP TSFTKDSYNG KDVSGGWFDC GDHVMYGQSQ GYASYVLALA
110 120 130 140 150
YAEFTEVSTT FILVTTPTTR KPTTTPMKSG KPNKVRDLLE ELRYEADFWV
160 170 180 190 200
KAAIDGNNFV TVKGDGNADH QKWVTAGAMS KLGSGEGGEP RCITGNANDG
210 220 230 240 250
FTSGLAAAML AVMARVDPDT ANQAKYLKAA KTAYSYAKSH KGVTNSQGFY
260 270 280 290 300
ESSWWDGRWE DGPFLAELEL YRTTGENSYK TAAIDRYDNL KFSLGEGTHF
310 320 330 340 350
MYSNVVPLSA VMAEAVFEET PHGMRKEAIG VLDLIYEEKA KDKIFQNPNG
360 370 380 390 400
MGSGKFPVRV PSGGAFLYAL SDKFNNTNEH MEMIEKNVSY LLGDNGSKKS
410 420 430 440 450
YVVGFSKNGA NAPSRPHHRG YYANEKRWRR SRRCSESSRK EQALGRYDCW

RLY
Length:453
Mass (Da):50,042
Last modified:November 1, 1991 - v1
Checksum:i7E861B76070D1186
GO

Sequence cautioni

The sequence AAA24895.1 differs from that shown. Reason: Frameshift at position 127.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58520 Genomic DNA. Translation: AAA24894.1.
M58520 Genomic DNA. Translation: AAA24895.1. Frameshift.
PIRiA39416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58520 Genomic DNA. Translation: AAA24894.1 .
M58520 Genomic DNA. Translation: AAA24895.1 . Frameshift.
PIRi A39416.

3D structure databases

ProteinModelPortali P23665.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view ]
Pfami PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "endAFS, a novel family E endoglucanase gene from Fibrobacter succinogenes AR1."
    Cavicchioli R., East P.D., Watson K.
    J. Bacteriol. 173:3265-3268(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AR1.
  2. "The involvement of transcriptional read-through from internal promoters in the expression of a novel endoglucanase gene FSendA, from Fibrobacter succinogenes AR1."
    Cavicchioli R., Watson K.
    Nucleic Acids Res. 19:1661-1669(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: RIBOSOMAL FRAMESHIFT.
    Strain: AR1.
  3. "Molecular cloning, expression, and characterization of endoglucanase genes from Fibrobacter succinogenes AR1."
    Cavicchioli R., Watson K.
    Appl. Environ. Microbiol. 57:359-365(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBSTRATES, SUBUNIT, SUBCELLULAR LOCATION, CHARACTERIZATION.
    Strain: AR1.

Entry informationi

Entry nameiGUNA_FIBSU
AccessioniPrimary (citable) accession number: P23665
Secondary accession number(s): P23664
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Expression of endA requires a ribosomal frameshift to allow contiguous translation of a 453 amino acid protein; this is suggested to take place at position 127 but could in fact occur anywhere between positions 82 and 127.Curated
On the basis of sequence similarities the C-terminal part of this sequence seems to be incorrect.Curated

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3