Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P23665

- GUNA_FIBSU

UniProt

P23665 - GUNA_FIBSU

Protein

Endoglucanase A

Gene

endA

Organism
Fibrobacter succinogenes (Bacteroides succinogenes)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    High levels of endoglucanase activity detected on acid-swollen cellulose, ball-milled cellulose, and carboxymethyl cellulose; moderate levels detected on filter paper, phosphoric acid-swollen cellulose, lichenan, and xylan.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    pH dependencei

    Optimum pH is 5.0.

    Temperature dependencei

    Optimum temperature is 37-39 degrees Celsius.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei417 – 4171PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase A (EC:3.2.1.4)
    Alternative name(s):
    Cellulase
    Endo-1,4-beta-glucanase
    Gene namesi
    Name:endA
    OrganismiFibrobacter succinogenes (Bacteroides succinogenes)
    Taxonomic identifieri833 [NCBI]
    Taxonomic lineageiBacteriaFibrobacteresFibrobacteralesFibrobacteraceaeFibrobacter

    Subcellular locationi

    Periplasm 1 Publication
    Note: 80% periplasmic, 16% extracellular upon overexpression in E.coli.

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 453427Endoglucanase APRO_0000007954Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP23665.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni115 – 12612Linker ("hinge") (Pro-Thr box)Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view]
    PfamiPF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23665-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNCRKYLLSG LAVFGLAATS AVAALSTDDY VEAAWMTTRF FGAQRSGQGP    50
    NWILDGTSNP TSFTKDSYNG KDVSGGWFDC GDHVMYGQSQ GYASYVLALA 100
    YAEFTEVSTT FILVTTPTTR KPTTTPMKSG KPNKVRDLLE ELRYEADFWV 150
    KAAIDGNNFV TVKGDGNADH QKWVTAGAMS KLGSGEGGEP RCITGNANDG 200
    FTSGLAAAML AVMARVDPDT ANQAKYLKAA KTAYSYAKSH KGVTNSQGFY 250
    ESSWWDGRWE DGPFLAELEL YRTTGENSYK TAAIDRYDNL KFSLGEGTHF 300
    MYSNVVPLSA VMAEAVFEET PHGMRKEAIG VLDLIYEEKA KDKIFQNPNG 350
    MGSGKFPVRV PSGGAFLYAL SDKFNNTNEH MEMIEKNVSY LLGDNGSKKS 400
    YVVGFSKNGA NAPSRPHHRG YYANEKRWRR SRRCSESSRK EQALGRYDCW 450
    RLY 453
    Length:453
    Mass (Da):50,042
    Last modified:November 1, 1991 - v1
    Checksum:i7E861B76070D1186
    GO

    Sequence cautioni

    The sequence AAA24895.1 differs from that shown. Reason: Frameshift at position 127.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58520 Genomic DNA. Translation: AAA24894.1.
    M58520 Genomic DNA. Translation: AAA24895.1. Frameshift.
    PIRiA39416.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58520 Genomic DNA. Translation: AAA24894.1 .
    M58520 Genomic DNA. Translation: AAA24895.1 . Frameshift.
    PIRi A39416.

    3D structure databases

    ProteinModelPortali P23665.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH9. Glycoside Hydrolase Family 9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view ]
    Pfami PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "endAFS, a novel family E endoglucanase gene from Fibrobacter succinogenes AR1."
      Cavicchioli R., East P.D., Watson K.
      J. Bacteriol. 173:3265-3268(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AR1.
    2. "The involvement of transcriptional read-through from internal promoters in the expression of a novel endoglucanase gene FSendA, from Fibrobacter succinogenes AR1."
      Cavicchioli R., Watson K.
      Nucleic Acids Res. 19:1661-1669(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: RIBOSOMAL FRAMESHIFT.
      Strain: AR1.
    3. "Molecular cloning, expression, and characterization of endoglucanase genes from Fibrobacter succinogenes AR1."
      Cavicchioli R., Watson K.
      Appl. Environ. Microbiol. 57:359-365(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBSTRATES, SUBUNIT, SUBCELLULAR LOCATION, CHARACTERIZATION.
      Strain: AR1.

    Entry informationi

    Entry nameiGUNA_FIBSU
    AccessioniPrimary (citable) accession number: P23665
    Secondary accession number(s): P23664
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Expression of endA requires a ribosomal frameshift to allow contiguous translation of a 453 amino acid protein; this is suggested to take place at position 127 but could in fact occur anywhere between positions 82 and 127.Curated
    On the basis of sequence similarities the C-terminal part of this sequence seems to be incorrect.Curated

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3