P23665 (GUNA_FIBSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase A
EC=3.2.1.4

Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase

Gene names

Name:

endA

Organism

Fibrobacter succinogenes (Bacteroides succinogenes)

Taxonomic identifier

833 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Fibrobacteres/Acidobacteria group › Fibrobacteres › Fibrobacteria › Fibrobacterales › Fibrobacteraceae › Fibrobacter

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High levels of endoglucanase activity detected on acid-swollen cellulose, ball-milled cellulose, and carboxymethyl cellulose; moderate levels detected on filter paper, phosphoric acid-swollen cellulose, lichenan, and xylan.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subunit structure	Monomer Probable. Ref.3
Subcellular location	Periplasm. Note: 80% periplasmic, 16% extracellular upon overexpression in E.coli. Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.
Caution	Expression of endA requires a ribosomal frameshift to allow contiguous translation of a 453 amino acid protein; this is suggested to take place at position 127 but could in fact occur anywhere between positions 82 and 127. On the basis of sequence similarities the C-terminal part of this sequence seems to be incorrect.
Biophysicochemical properties	pH dependence: Optimum pH is 5.0. Temperature dependence: Optimum temperature is 37-39 degrees Celsius.
Sequence caution	The sequence AAA24895.1 differs from that shown. Reason: Frameshift at position 127.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Periplasm
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 453	427	Endoglucanase A		PRO_0000007954
Regions
	Region	115 – 126	12	Linker ("hinge") (Pro-Thr box)
Sites
	Active site	417	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23665 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 7E861B76070D1186	FASTA	453	50,042
	10 20 30 40 50 60 MNCRKYLLSG LAVFGLAATS AVAALSTDDY VEAAWMTTRF FGAQRSGQGP NWILDGTSNP 70 80 90 100 110 120 TSFTKDSYNG KDVSGGWFDC GDHVMYGQSQ GYASYVLALA YAEFTEVSTT FILVTTPTTR 130 140 150 160 170 180 KPTTTPMKSG KPNKVRDLLE ELRYEADFWV KAAIDGNNFV TVKGDGNADH QKWVTAGAMS 190 200 210 220 230 240 KLGSGEGGEP RCITGNANDG FTSGLAAAML AVMARVDPDT ANQAKYLKAA KTAYSYAKSH 250 260 270 280 290 300 KGVTNSQGFY ESSWWDGRWE DGPFLAELEL YRTTGENSYK TAAIDRYDNL KFSLGEGTHF 310 320 330 340 350 360 MYSNVVPLSA VMAEAVFEET PHGMRKEAIG VLDLIYEEKA KDKIFQNPNG MGSGKFPVRV 370 380 390 400 410 420 PSGGAFLYAL SDKFNNTNEH MEMIEKNVSY LLGDNGSKKS YVVGFSKNGA NAPSRPHHRG 430 440 450 YYANEKRWRR SRRCSESSRK EQALGRYDCW RLY

References

[1]	"endAFS, a novel family E endoglucanase gene from Fibrobacter succinogenes AR1." Cavicchioli R., East P.D., Watson K. J. Bacteriol. 173:3265-3268(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AR1.
[2]	"The involvement of transcriptional read-through from internal promoters in the expression of a novel endoglucanase gene FSendA, from Fibrobacter succinogenes AR1." Cavicchioli R., Watson K. Nucleic Acids Res. 19:1661-1669(1991) [PubMed] [Europe PMC] [Abstract] Cited for: RIBOSOMAL FRAMESHIFT. Strain: AR1.
[3]	"Molecular cloning, expression, and characterization of endoglucanase genes from Fibrobacter succinogenes AR1." Cavicchioli R., Watson K. Appl. Environ. Microbiol. 57:359-365(1991) [PubMed] [Europe PMC] [Abstract] Cited for: SUBSTRATES, SUBUNIT, SUBCELLULAR LOCATION, CHARACTERIZATION. Strain: AR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58520 Genomic DNA. Translation: AAA24894.1. M58520 Genomic DNA. Translation: AAA24895.1. Frameshift.
PIR	A39416.
3D structure databases
ProteinModelPortal	P23665.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNA_FIBSU

Accession

Primary (citable) accession number: P23665
Secondary accession number(s): P23664

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program