Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P23660

- GUNA_RUMAL

UniProt

P23660 - GUNA_RUMAL

Protein

Endoglucanase A

Gene

celA

Organism
Ruminococcus albus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Hydrolyzes both carboxymethylcellulose and xylan. Probably has a role in hydrolyzing oligosaccharides derived from cellulose, which are transported across the cell wall.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
    Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei169 – 1691Proton donorBy similarity
    Active sitei293 – 2931NucleophileBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW
    2. xylan catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation, Xylan degradation

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase A (EC:3.2.1.4)
    Alternative name(s):
    Cellulase A
    Endo-1,4-beta-glucanase A
    Short name:
    EGA
    Gene namesi
    Name:celA
    OrganismiRuminococcus albus
    Taxonomic identifieri1264 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminococcus

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 364364Endoglucanase APRO_0000184051Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP23660.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00150. Cellulase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23660-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKPDKDADR LTTLDLARSG EVRDISAMEL VGEMKTGWNL GNSLDATGAP    50
    GNASEVNWGN PKTTKEMIDA VYNKGFDVIR IPVTWGGHVG DAPDYKIDDE 100
    WIARVQEVVN YAYDDGAYVI INSHHEEDWR IPDNEHIDAV DEKTAAIWKQ 150
    VAERFKDYGD HLIFEGLNEP RVKGSPQEWN GGTEEGRRCV DRLNKTFLDT 200
    VRATGGNNEK RLLLMTTYAS SSMSNVIKDT AIPEDDHIGF SIHAYTPYAF 250
    TYNANADWEL FHWDDSHDGE LVSLMTNLKE NYLDKDIPVI ITEYGAVNKD 300
    NNDEDRAKWV SSYIEYAELL GGIPCVWWDN GYYSSGNELF GIFDRNTCTW 350
    FTDTVTDAII ENAK 364
    Length:364
    Mass (Da):41,218
    Last modified:November 1, 1991 - v1
    Checksum:iF8DCD2E2E55781F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54931 Genomic DNA. Translation: CAA38692.1.
    PIRiS12017.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54931 Genomic DNA. Translation: CAA38692.1 .
    PIRi S12017.

    3D structure databases

    ProteinModelPortali P23660.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the Ruminococcus albus SY3 endoglucanase genes celA and celB."
      Poole D.M., Hazlewood G.P., Laurie J.I., Barker P.J., Gilbert H.J.
      Mol. Gen. Genet. 223:217-223(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-14.
      Strain: SY3.

    Entry informationi

    Entry nameiGUNA_RUMAL
    AccessioniPrimary (citable) accession number: P23660
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3