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P23660 (GUNA_RUMAL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase A

EC=3.2.1.4
Alternative name(s):
Cellulase A
Endo-1,4-beta-glucanase A
Short name=EGA
Gene names
Name:celA
OrganismRuminococcus albus
Taxonomic identifier1264 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminococcus

Protein attributes

Sequence length364 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes both carboxymethylcellulose and xylan. Probably has a role in hydrolyzing oligosaccharides derived from cellulose, which are transported across the cell wall.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
Xylan degradation
   Cellular componentCytoplasm
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

xylan catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 364364Endoglucanase A
PRO_0000184051

Sites

Active site1691Proton donor By similarity
Active site2931Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P23660 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: F8DCD2E2E55781F7

FASTA36441,218
        10         20         30         40         50         60 
MRKPDKDADR LTTLDLARSG EVRDISAMEL VGEMKTGWNL GNSLDATGAP GNASEVNWGN 

        70         80         90        100        110        120 
PKTTKEMIDA VYNKGFDVIR IPVTWGGHVG DAPDYKIDDE WIARVQEVVN YAYDDGAYVI 

       130        140        150        160        170        180 
INSHHEEDWR IPDNEHIDAV DEKTAAIWKQ VAERFKDYGD HLIFEGLNEP RVKGSPQEWN 

       190        200        210        220        230        240 
GGTEEGRRCV DRLNKTFLDT VRATGGNNEK RLLLMTTYAS SSMSNVIKDT AIPEDDHIGF 

       250        260        270        280        290        300 
SIHAYTPYAF TYNANADWEL FHWDDSHDGE LVSLMTNLKE NYLDKDIPVI ITEYGAVNKD 

       310        320        330        340        350        360 
NNDEDRAKWV SSYIEYAELL GGIPCVWWDN GYYSSGNELF GIFDRNTCTW FTDTVTDAII 


ENAK 

« Hide

References

[1]"Nucleotide sequence of the Ruminococcus albus SY3 endoglucanase genes celA and celB."
Poole D.M., Hazlewood G.P., Laurie J.I., Barker P.J., Gilbert H.J.
Mol. Gen. Genet. 223:217-223(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-14.
Strain: SY3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54931 Genomic DNA. Translation: CAA38692.1.
PIRS12017.

3D structure databases

ProteinModelPortalP23660.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUNA_RUMAL
AccessionPrimary (citable) accession number: P23660
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 16, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries