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P23659 (GUNZ_CLOSR) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase Z

EC=3.2.1.4
Alternative name(s):
Avicelase I
Endo-1,4-beta-glucanase
Thermoactive cellulase
Gene names
Name:celZ
OrganismClostridium stercorarium
Taxonomic identifier1510 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeunclassified Ruminococcaceae

Protein attributes

Sequence length986 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Contains 2 CBM3 (carbohydrate binding type-3) domains.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainRepeat
Signal
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

cellulose binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.1
Chain26 – 986961Endoglucanase Z
PRO_0000007948

Regions

Domain481 – 642162CBM3 1
Repeat651 – 73888Domain B
Repeat744 – 83188Domain B'
Domain836 – 986151CBM3 2

Sites

Active site4001 By similarity
Active site4381 By similarity
Active site4471 By similarity

Sequences

Sequence LengthMass (Da)Tools
P23659 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 1802E09B22923690

FASTA986109,512
        10         20         30         40         50         60 
MRKFYSFAII ISLLVTGLFI HTPKAEAAGY NYGEALQKAI MFYEFQRSGK LPENKRDNWR 

        70         80         90        100        110        120 
GDSGLNDGAD VGLDLTGGWY DAGDHVKFNL PMAYSQTMLA WAAYEAEEAL ERSGQMGYLL 

       130        140        150        160        170        180 
DAIKWVSDYL IKCHPSPNVF YYQVGDGHLD HSWWGPAEVM QMDRPAYKVD LANPGSTVVA 

       190        200        210        220        230        240 
EAAAALASAA VVFADRDPAY AATCIQHAKE LYNFAEITKS DSGYTAASGF YDSHSGFYDE 

       250        260        270        280        290        300 
LSWAGVWLYL ATGDETYLNK AEQYVAYWGT EPQTNIISYK WAHCWDDVHY GACLLLAKIT 

       310        320        330        340        350        360 
GKQIYKEAIE RHLDYWSVGY NGERVHYTPK GLAWLDSWGS LRYATTTAFL ASVYADWEGC 

       370        380        390        400        410        420 
SREKAAIYND FAKQQIDYAL GSSGRSYVVG FGVNPPKRPH HRTAHSSWAD SMSVPDYHRH 

       430        440        450        460        470        480 
VLIGALVGGP GKDDSYTDDI NNYINNEVAC DYNAGFVGAL AKMYEDYGGS PIPDLNAFEE 

       490        500        510        520        530        540 
ITNDEFFVMA GINASGQNFI EIKALLHNQS GWPARVADKL SFRYFVDLTE LIEAGYSASD 

       550        560        570        580        590        600 
VTITTNYNAG AKVTGLHPWN EAENIYYVNV DFTGTKIYPG GQSAYRKEVQ FRIAAPQNTN 

       610        620        630        640        650        660 
FWNNDNDYSF RDIKGVTSGN TVKTVYIPVY DDGVLVFGVE PEGGSGENNS SISITNATFD 

       670        680        690        700        710        720 
KNPAKQENIQ VVMNLNGNTL NGIKYGNTYL REGTDYTVSG DTVTILKSFL NSFDTSTVQL 

       730        740        750        760        770        780 
IFDFSAGRDP VLTVNIIDTT TSASIVPTTA DFDKNPDASR DVKVKLVPNG NTLLAVKKDG 

       790        800        810        820        830        840 
EALVLGRDYS IDGDEVTIFR EYLADQPVGR VTLTFDFDRG TDPVLTINIT DSRQVETGVI 

       850        860        870        880        890        900 
QIQMFNGNTS DKTNGIMPRY RLTNTGTTPI RLSDVKIRYY YTIDGEKDQN FWCDWSSVGS 

       910        920        930        940        950        960 
NNITGTFVKM AEPKEGADYY LETGFTDGAG YLQPNQSIEV QNRFSKADWT DYIQTNDYSF 

       970        980 
STNTSYGSND RITVYISGVL VSGIEP 

« Hide

References

[1]"Sequence analysis of the Clostridium stercorarium celZ gene encoding a thermoactive cellulase (Avicelase I): identification of catalytic and cellulose-binding domains."
Jauris S., Ruecknagel K.P., Schwarz W.H., Kratzsch P., Bronnenmeier K., Staudenbauer W.L.
Mol. Gen. Genet. 223:258-267(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-36 AND 475-486.
Strain: NCIB 11745.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55299 Genomic DNA. Translation: CAA39010.1. Sequence problems.
PIRS12021.

3D structure databases

ProteinModelPortalP23659.
SMRP23659. Positions 30-641.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM3. Carbohydrate-Binding Module Family 3.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.50.10.10. 1 hit.
2.60.40.10. 2 hits.
2.60.40.710. 2 hits.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR008965. Carb-bd_dom.
IPR005102. Carbo-bd_X2.
IPR001956. CBD_3.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PfamPF00942. CBM_3. 2 hits.
PF03442. CBM_X2. 2 hits.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTSM01067. CBM_3. 2 hits.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
SSF49384. SSF49384. 2 hits.
SSF81296. SSF81296. 2 hits.
PROSITEPS51172. CBM3. 2 hits.
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUNZ_CLOSR
AccessionPrimary (citable) accession number: P23659
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 16, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries