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Protein

Serine/threonine-protein kinase fused

Gene

fu

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable serine/threonine-protein kinase; maternally required for correct patterning in the posterior part of each embryonic metamere. May be involved in control of cell division during metamorphosis and ovarian development. May interact with costal-2.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei125 – 1251Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: FlyBase

GO - Biological processi

  • germarium-derived egg chamber formation Source: FlyBase
  • intraciliary transport Source: FlyBase
  • negative regulation of BMP signaling pathway Source: FlyBase
  • positive regulation of hh target transcription factor activity Source: FlyBase
  • positive regulation of protein ubiquitination Source: FlyBase
  • positive regulation of transcription factor import into nucleus Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of protein stability Source: FlyBase
  • regulation of proteolysis Source: FlyBase
  • segment polarity determination Source: FlyBase
  • smoothened signaling pathway Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Segmentation polarity protein, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 1994.
SignaLinkiP23647.

Protein family/group databases

MEROPSiM12.A08.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase fused (EC:2.7.11.1)
Gene namesi
Name:fu
ORF Names:CG6551
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0001079. fu.

Subcellular locationi

GO - Cellular componenti

  • cilium Source: GOC
  • cytoplasm Source: FlyBase
  • Hedgehog signaling complex Source: FlyBase
  • membrane Source: FlyBase
  • protein complex Source: FlyBase
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi139 – 1413Missing in FU-62. 1 Publication
Mutagenesisi147 – 1471A → T in FU-H63. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 805805Serine/threonine-protein kinase fusedPRO_0000085954Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei422 – 4221Phosphoserine1 Publication
Modified residuei429 – 4291Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP23647.
PRIDEiP23647.

PTM databases

iPTMnetiP23647.

Expressioni

Tissue specificityi

Expressed in all imaginal disks, higher level in wing disk.1 Publication

Developmental stagei

Expressed both maternally and zygotically, low expression is present in males, larvae and pupae.

Gene expression databases

BgeeiFBgn0001079.
GenevisibleiP23647. DM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ciP195388EBI-165536,EBI-94976
cosO168444EBI-165536,EBI-102069
lackQ9V8535EBI-165536,EBI-133520
tkvQ95SI04EBI-165536,EBI-3401975

Protein-protein interaction databases

BioGridi59167. 46 interactions.
DIPiDIP-492N.
IntActiP23647. 9 interactions.
MINTiMINT-342659.
STRINGi7227.FBpp0074373.

Structurei

3D structure databases

ProteinModelPortaliP23647.
SMRiP23647. Positions 2-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 254251Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0597. Eukaryota.
ENOG410XRQ6. LUCA.
InParanoidiP23647.
KOiK06228.
OrthoDBiEOG091G04G9.
PhylomeDBiP23647.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23647-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRYAVSSLV GQGSFGCVYK ATRKDDSKVV AIKVISKRGR ATKELKNLRR
60 70 80 90 100
ECDIQARLKH PHVIEMIESF ESKTDLFVVT EFALMDLHRY LSYNGAMGEE
110 120 130 140 150
PARRVTGHLV SALYYLHSNR ILHRDLKPQN VLLDKNMHAK LCDFGLARNM
160 170 180 190 200
TLGTHVLTSI KGTPLYMAPE LLAEQPYDHH ADMWSLGCIA YESMAGQPPF
210 220 230 240 250
CASSILHLVK MIKHEDVKWP STLTSECRSF LQGLLEKDPG LRISWTQLLC
260 270 280 290 300
HPFVEGRIFI AETQAEAAKE SPFTNPEAKV KSSKQSDPEV GDLDEALAAL
310 320 330 340 350
DFGESRQENL TTSRDSINAI APSDVEHLET DVEDNMQRVV VPFADLSYRD
360 370 380 390 400
LSGVRAMPMV HQPVINSHTC FVSGNSNMIL NHMNDNFDFQ ASLRGGGVAA
410 420 430 440 450
KPIVAPTVRQ SRSKDLEKRK LSQNLDNFSV RLGHSVDHEA QRKATEIATQ
460 470 480 490 500
EKHNQENKPP AEAISYANSQ PPQQQPQQLK HSMHSTNEEK LSSDNTPPCL
510 520 530 540 550
LPGWDSCDES QSPPIENDEW LAFLNRSVQE LLDGELDSLK QHNLVSIIVA
560 570 580 590 600
PLRNSKAIPR VLKSVAQLLS LPFVLVDPVL IVDLELIRNV YVDVKLVPNL
610 620 630 640 650
MYACKLLLSH KQLSDSAASA PLTTGSLSRT LRSIPELTVE ELETACSLYE
660 670 680 690 700
LVCHLVHLQQ QFLTQFCDAV AILAASDLFL NFLTHDFRQS DSDAASVRLA
710 720 730 740 750
GCMLALMGCV LRELPENAEL VERIVFNPRL NFVSLLQSRH HLLRQRSCQL
760 770 780 790 800
LRLLARFSLR GVQRIWNGEL RFALQQLSEH HSYPALRGEA AQTLDEISHF

TFFVT
Length:805
Mass (Da):90,347
Last modified:December 1, 2000 - v2
Checksum:iA7804E2749B79D0C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91L → M in AAF48871 (PubMed:10731132).Curated
Sequence conflicti15 – 151F → L in CAA56640 (PubMed:8155575).Curated
Sequence conflicti15 – 151F → L in AAA28552 (PubMed:8155575).Curated
Sequence conflicti37 – 371K → KVSGAGQVIKQQVHTTSSHH IHVLQ (PubMed:2168522).Curated
Sequence conflicti174 – 1752EQ → DE (PubMed:8155575).Curated
Sequence conflicti174 – 1752EQ → DE (PubMed:2168522).Curated
Sequence conflicti225 – 2251S → C (PubMed:8155575).Curated
Sequence conflicti225 – 2251S → C (PubMed:2168522).Curated
Sequence conflicti326 – 3261E → D in CAA56640 (PubMed:8155575).Curated
Sequence conflicti326 – 3261E → D in AAA28552 (PubMed:8155575).Curated
Sequence conflicti397 – 3971G → V in CAA56640 (PubMed:8155575).Curated
Sequence conflicti397 – 3971G → V in AAA28552 (PubMed:8155575).Curated
Sequence conflicti708 – 7081G → S in CAA56640 (PubMed:8155575).Curated
Sequence conflicti708 – 7081G → S in AAA28552 (PubMed:8155575).Curated
Sequence conflicti744 – 7441R → G in CAA56640 (PubMed:8155575).Curated
Sequence conflicti744 – 7441R → G in AAA28552 (PubMed:8155575).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80468 Genomic DNA. Translation: CAA56640.1.
L34782 Genomic DNA. Translation: AAA28552.1.
AE014298 Genomic DNA. Translation: AAF48871.1.
S69165 Genomic DNA. Translation: AAB29840.1.
S69166 Genomic DNA. Translation: AAB29841.1.
X55759 Genomic DNA. Translation: CAA39285.1.
PIRiS11380.
RefSeqiNP_477499.1. NM_058151.5.

Genome annotation databases

GeneIDi32855.
KEGGidme:Dmel_CG6551.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80468 Genomic DNA. Translation: CAA56640.1.
L34782 Genomic DNA. Translation: AAA28552.1.
AE014298 Genomic DNA. Translation: AAF48871.1.
S69165 Genomic DNA. Translation: AAB29840.1.
S69166 Genomic DNA. Translation: AAB29841.1.
X55759 Genomic DNA. Translation: CAA39285.1.
PIRiS11380.
RefSeqiNP_477499.1. NM_058151.5.

3D structure databases

ProteinModelPortaliP23647.
SMRiP23647. Positions 2-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59167. 46 interactions.
DIPiDIP-492N.
IntActiP23647. 9 interactions.
MINTiMINT-342659.
STRINGi7227.FBpp0074373.

Protein family/group databases

MEROPSiM12.A08.

PTM databases

iPTMnetiP23647.

Proteomic databases

PaxDbiP23647.
PRIDEiP23647.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi32855.
KEGGidme:Dmel_CG6551.

Organism-specific databases

CTDi32855.
FlyBaseiFBgn0001079. fu.

Phylogenomic databases

eggNOGiKOG0597. Eukaryota.
ENOG410XRQ6. LUCA.
InParanoidiP23647.
KOiK06228.
OrthoDBiEOG091G04G9.
PhylomeDBiP23647.

Enzyme and pathway databases

BRENDAi2.7.11.1. 1994.
SignaLinkiP23647.

Miscellaneous databases

GenomeRNAii32855.
PROiP23647.

Gene expression databases

BgeeiFBgn0001079.
GenevisibleiP23647. DM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUSED_DROME
AccessioniPrimary (citable) accession number: P23647
Secondary accession number(s): Q26346
, Q26347, Q27412, Q9VWR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: December 1, 2000
Last modified: September 7, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.