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Protein

Neurogenic protein big brain

Gene

bib

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for proper differentiation of ectoderm. Acts synergistically with neurogenic locus proteins Notch and Delta during the separation of neural and epidermal cell lineages in response to the lateral inhibition signal. Voltage-insensitive monovalent cation channel. Ion transport is blocked by the presence of divalent cations.5 Publications

GO - Molecular functioni

  • cation channel activity Source: FlyBase
  • channel activity Source: FlyBase
  • glycerol channel activity Source: GO_Central
  • water channel activity Source: GO_Central

GO - Biological processi

  • cellular water homeostasis Source: GO_Central
  • ectoderm development Source: FlyBase
  • lateral inhibition Source: FlyBase
  • mesoderm development Source: FlyBase
  • nervous system development Source: FlyBase
  • Notch signaling pathway Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • regulation of imaginal disc-derived wing size Source: FlyBase
  • single organismal cell-cell adhesion Source: FlyBase
  • transmembrane transport Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Ion channel

Keywords - Biological processi

Differentiation, Ion transport, Neurogenesis, Transport

Enzyme and pathway databases

SignaLinkiP23645.

Protein family/group databases

TCDBi1.A.8.8.3. the major intrinsic protein (mip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurogenic protein big brain
Gene namesi
Name:bib
ORF Names:CG4722
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000180. bib.

Subcellular locationi

  • Membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7171CytoplasmicSequence analysisAdd
BLAST
Transmembranei72 – 9322HelicalSequence analysisAdd
BLAST
Topological domaini94 – 974ExtracellularSequence analysis
Transmembranei98 – 11821HelicalSequence analysisAdd
BLAST
Topological domaini119 – 14325CytoplasmicSequence analysisAdd
BLAST
Transmembranei144 – 16724HelicalSequence analysisAdd
BLAST
Topological domaini168 – 18922ExtracellularSequence analysisAdd
BLAST
Transmembranei190 – 20819HelicalSequence analysisAdd
BLAST
Topological domaini209 – 2135CytoplasmicSequence analysis
Transmembranei214 – 23421HelicalSequence analysisAdd
BLAST
Topological domaini235 – 25622ExtracellularSequence analysisAdd
BLAST
Transmembranei257 – 27317HelicalSequence analysisAdd
BLAST
Topological domaini274 – 696423CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • adherens junction Source: FlyBase
  • cytoplasmic vesicle Source: FlyBase
  • integral component of membrane Source: FlyBase
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi71 – 711E → D: Partial loss of transport activity and increased sensitivity to blocking by the magnesium ion. 2 Publications
Mutagenesisi71 – 711E → K: Loss of expression in cell membrane. 2 Publications
Mutagenesisi71 – 711E → N: Loss of transport activity. 2 Publications
Mutagenesisi71 – 711E → Q: Loss of transport activity. 2 Publications
Mutagenesisi253 – 2531D → N: No effect on transport activity. 1 Publication
Mutagenesisi274 – 2741E → Q: No effect on transport activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 696696Neurogenic protein big brainPRO_0000064097Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 461Phosphoserine1 Publication
Modified residuei47 – 471Phosphothreonine1 Publication
Modified residuei273 – 2731Phosphotyrosine; by SrcSequence analysis
Modified residuei300 – 3001Phosphoserine1 Publication
Modified residuei367 – 3671Phosphotyrosine; by AblSequence analysis
Modified residuei384 – 3841Phosphotyrosine; by SrcSequence analysis
Modified residuei394 – 3941Phosphoserine1 Publication
Modified residuei478 – 4781Phosphotyrosine; by SrcSequence analysis
Modified residuei576 – 5761Phosphoserine1 Publication
Modified residuei610 – 6101Phosphotyrosine; by AblSequence analysis

Post-translational modificationi

Phosphorylated at its C-terminus.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP23645.

PTM databases

iPTMnetiP23645.

Expressioni

Tissue specificityi

Detected in all tissues with neurogenic abilities, for example the neurogenic ectoderm.1 Publication

Gene expression databases

BgeeiP23645.
ExpressionAtlasiP23645. differential.
GenevisibleiP23645. DM.

Interactioni

Protein-protein interaction databases

BioGridi60424. 4 interactions.
DIPiDIP-21188N.
IntActiP23645. 1 interaction.
MINTiMINT-1607360.
STRINGi7227.FBpp0303019.

Structurei

3D structure databases

ProteinModelPortaliP23645.
SMRiP23645. Positions 57-277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi126 – 1283NPA 1
Motifi238 – 2403NPA 2

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi439 – 686248Gln-richAdd
BLAST
Compositional biasi591 – 62333Pro-richAdd
BLAST

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
GeneTreeiENSGT00760000119223.
InParanoidiP23645.
OrthoDBiEOG72NRQ0.
PhylomeDBiP23645.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23645-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADESLHTVP LEHNIDYHIV TLFERLEAMR KDSHGGGHGV NNRLSSTLQA
60 70 80 90 100
PKRSMQAEIR TLEFWRSIIS ECLASFMYVF IVCGAAAGVG VGASVSSVLL
110 120 130 140 150
ATALASGLAM ATLTQCFLHI SGAHINPAVT LALCVVRSIS PIRAAMYITA
160 170 180 190 200
QCGGGIAGAA LLYGVTVPGY QGNLQAAISH SAALAAWERF GVEFILTFLV
210 220 230 240 250
VLCYFVSTDP MKKFMGNSAA SIGCAYSACC FVSMPYLNPA RSLGPSFVLN
260 270 280 290 300
KWDSHWVYWF GPLVGGMASG LVYEYIFNSR NRNLRHNKGS IDNDSSSIHS
310 320 330 340 350
EDELNYDMDM EKPNKYQQSQ GTYPRGQSNG NGGGQAAGNG QHQAANMGQM
360 370 380 390 400
PGVVANAGQG NYCQNLYTAP PLSSKYDQQQ EPLYGGTRSL YCRSPTLTRS
410 420 430 440 450
NLNRSQSVYA KSNTAINRDI VPRPGPLVPA QSLYPMRTQQ QQQQQQQQQQ
460 470 480 490 500
QVAPAPQSSH LQNQNVQNQM QQRSESIYGM RGSMRGQQQP IQQQQQQQQQ
510 520 530 540 550
QQLQQQQPNM GVQQQQMQPP PQMMSDPQQQ PQGFQPVYGT RTNPTPMDGN
560 570 580 590 600
HKYDRRDPQQ MYGVTGPRNR GQSAQSDDSS YGSYHGSAVT PPARHPSVEP
610 620 630 640 650
SPPPPPMLMY APPPQPNAAH PQPIRTQSER KVSAPVVVSQ PAACAVTYTT
660 670 680 690
SQGSAVTAQQ QQQQQQQQQQ QQQQQQQQMM MQQQQQHYGM LPLRPN
Length:696
Mass (Da):76,509
Last modified:July 19, 2005 - v2
Checksum:i14DEA6064FAFAB72
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti198 – 1981F → S in CAB37863 (PubMed:1692392).Curated
Sequence conflicti454 – 4541P → S in CAB37863 (PubMed:1692392).Curated
Sequence conflicti492 – 4921Missing in CAB37863 (PubMed:1692392).Curated
Sequence conflicti678 – 6781Q → QQQQQQ (PubMed:1692392).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53275 mRNA. Translation: CAB37863.1.
AE014134 Genomic DNA. Translation: AAF52844.1.
AY051981 mRNA. Translation: AAK93405.1.
PIRiS09699.
RefSeqiNP_476837.1. NM_057489.5.
UniGeneiDm.3236.

Genome annotation databases

EnsemblMetazoaiFBtr0079929; FBpp0079519; FBgn0000180.
GeneIDi34330.
KEGGidme:Dmel_CG4722.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53275 mRNA. Translation: CAB37863.1.
AE014134 Genomic DNA. Translation: AAF52844.1.
AY051981 mRNA. Translation: AAK93405.1.
PIRiS09699.
RefSeqiNP_476837.1. NM_057489.5.
UniGeneiDm.3236.

3D structure databases

ProteinModelPortaliP23645.
SMRiP23645. Positions 57-277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60424. 4 interactions.
DIPiDIP-21188N.
IntActiP23645. 1 interaction.
MINTiMINT-1607360.
STRINGi7227.FBpp0303019.

Protein family/group databases

TCDBi1.A.8.8.3. the major intrinsic protein (mip) family.

PTM databases

iPTMnetiP23645.

Proteomic databases

PaxDbiP23645.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0079929; FBpp0079519; FBgn0000180.
GeneIDi34330.
KEGGidme:Dmel_CG4722.

Organism-specific databases

CTDi34330.
FlyBaseiFBgn0000180. bib.

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
GeneTreeiENSGT00760000119223.
InParanoidiP23645.
OrthoDBiEOG72NRQ0.
PhylomeDBiP23645.

Enzyme and pathway databases

SignaLinkiP23645.

Miscellaneous databases

GenomeRNAii34330.
PROiP23645.

Gene expression databases

BgeeiP23645.
ExpressionAtlasiP23645. differential.
GenevisibleiP23645. DM.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Similarity of the product of the Drosophila neurogenic gene big brain to transmembrane channel proteins."
    Rao Y., Jan L.Y., Jan Y.N.
    Nature 345:163-167(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "The big brain gene of Drosophila functions to control the number of neuronal precursors in the peripheral nervous system."
    Rao Y., Bodmer R., Jan L.Y., Jan Y.N.
    Development 116:31-40(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The Drosophila neurogenic gene big brain, which encodes a membrane-associated protein, acts cell autonomously and can act synergistically with Notch and Delta."
    Doherty D., Jan L.Y., Jan Y.N.
    Development 124:3881-3893(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  7. "Regulated cationic channel function in Xenopus oocytes expressing Drosophila big brain."
    Yanochko G.M., Yool A.J.
    J. Neurosci. 22:2530-2540(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF GLU-71.
  8. "Block by extracellular divalent cations of Drosophila big brain channels expressed in Xenopus oocytes."
    Yanochko G.M., Yool A.J.
    Biophys. J. 86:1470-1478(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLU-71; ASP-253 AND GLU-274.
  9. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46; THR-47; SER-300; SER-394 AND SER-576, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiBIB_DROME
AccessioniPrimary (citable) accession number: P23645
Secondary accession number(s): Q9VL53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: July 19, 2005
Last modified: July 6, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Separation of neuroblasts from the ectoderm into the inner part of embryo is one of the first steps of CNS development in insects, this process is under control of the neurogenic genes. Mutation in bib gene underlies "big brain" development defect.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.