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Protein

Mannan polymerase I complex VAN1 subunit

Gene

VAN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in regulation of the phosphorylation of a number of proteins, some of which appear to be important in cell growth control.
The M-Pol I complex possesses alpha-1,6-mannosyltransferase activity and is probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins.

GO - Molecular functioni

  • alpha-1,6-mannosyltransferase activity Source: UniProtKB

GO - Biological processi

  • cell wall mannoprotein biosynthetic process Source: UniProtKB
  • protein N-linked glycosylation Source: SGD
  • regulation of growth Source: UniProtKB-KW
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Growth regulation, Sporulation

Enzyme and pathway databases

BioCyciYEAST:YML115C-MONOMER.

Protein family/group databases

CAZyiGT62. Glycosyltransferase Family 62.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan polymerase I complex VAN1 subunit
Short name:
M-pol I subunit VAN1
Alternative name(s):
Vanadate resistance protein
Gene namesi
Name:VAN1
Synonyms:VRG7
Ordered Locus Names:YML115C
ORF Names:YM8339.04C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML115C.
SGDiS000004583. VAN1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6464CytoplasmicSequence analysisAdd
BLAST
Transmembranei65 – 8117Helical; Signal-anchor for type II membrane proteinAdd
BLAST
Topological domaini82 – 535454LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • alpha-1,6-mannosyltransferase complex Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi361 – 3611D → A: Reduced activity of the M-Pol I complex. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535Mannan polymerase I complex VAN1 subunitPRO_0000193672Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei25 – 251PhosphoserineCombined sources
Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence analysis
Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP23642.

PTM databases

iPTMnetiP23642.

Interactioni

Subunit structurei

Component of the M-Pol I complex which contains MNN9 and VAN1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MNN9P391073EBI-20237,EBI-11082

Protein-protein interaction databases

BioGridi35089. 37 interactions.
DIPiDIP-886N.
IntActiP23642. 1 interaction.
MINTiMINT-522083.

Structurei

3D structure databases

ProteinModelPortaliP23642.
SMRiP23642. Positions 172-514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ANP1/MMN9/VAN1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000005201.
HOGENOMiHOG000167461.
InParanoidiP23642.
KOiK05530.
OMAiGIRRKLM.
OrthoDBiEOG092C2GA4.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

P23642-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGMFFNLRSN IKKKAMDNGL SLPISRNGSS NNIKDKRSEH NSNSLKGKYR
60 70 80 90 100
YQPRSTPSKF QLTVSITSLI IIAVLSLYLF ISFLSGMGIG VSTQNGRSLL
110 120 130 140 150
GSSKSSENYK TIDLEDEEYY DYDFEDIDPE VISKFDDGVQ HYLISQFGSE
160 170 180 190 200
VLTPKDDEKY QRELNMLFDS TVEEYDLSNF EGAPNGLETR DHILLCIPLR
210 220 230 240 250
NAADVLPLMF KHLMNLTYPH ELIDLAFLVS DCSEGDTTLD ALIAYSRHLQ
260 270 280 290 300
NGTLSQIFQE IDAVIDSQTK GTDKLYLKYM DEGYINRVHQ AFSPPFHENY
310 320 330 340 350
DKPFRSVQIF QKDFGQVIGQ GFSDRHAVKV QGIRRKLMGR ARNWLTANAL
360 370 380 390 400
KPYHSWVYWR DADVELCPGS VIQDLMSKNY DVIVPNVWRP LPTFLGTEQP
410 420 430 440 450
YDLNSWMESQ EALALAKTLD EDDVIVEGYA EYPTWRVHLA YIRDAEGDPN
460 470 480 490 500
EAVDLDGVGG VSILAKAKIF RNGVQFPAFT FENHAETEAF GKMAKKMGYR
510 520 530
VGGLPHYTIW HIYEPSDDDL KEIASREREK RRQSE
Length:535
Mass (Da):61,092
Last modified:October 1, 1996 - v3
Checksum:i92767D0E4C3ECCED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871M → L in AAA35204 (PubMed:2137555).Curated
Sequence conflicti140 – 1401Q → L in AAA35204 (PubMed:2137555).Curated
Sequence conflicti281 – 2811D → E in AAA35204 (PubMed:2137555).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33957 Genomic DNA. Translation: AAA35204.1.
Z49210 Genomic DNA. Translation: CAA89103.1.
BK006946 Genomic DNA. Translation: DAA09783.1.
PIRiS53957.
RefSeqiNP_013592.1. NM_001182477.1.

Genome annotation databases

EnsemblFungiiYML115C; YML115C; YML115C.
GeneIDi854925.
KEGGisce:YML115C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33957 Genomic DNA. Translation: AAA35204.1.
Z49210 Genomic DNA. Translation: CAA89103.1.
BK006946 Genomic DNA. Translation: DAA09783.1.
PIRiS53957.
RefSeqiNP_013592.1. NM_001182477.1.

3D structure databases

ProteinModelPortaliP23642.
SMRiP23642. Positions 172-514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35089. 37 interactions.
DIPiDIP-886N.
IntActiP23642. 1 interaction.
MINTiMINT-522083.

Protein family/group databases

CAZyiGT62. Glycosyltransferase Family 62.

PTM databases

iPTMnetiP23642.

Proteomic databases

MaxQBiP23642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML115C; YML115C; YML115C.
GeneIDi854925.
KEGGisce:YML115C.

Organism-specific databases

EuPathDBiFungiDB:YML115C.
SGDiS000004583. VAN1.

Phylogenomic databases

GeneTreeiENSGT00390000005201.
HOGENOMiHOG000167461.
InParanoidiP23642.
KOiK05530.
OMAiGIRRKLM.
OrthoDBiEOG092C2GA4.

Enzyme and pathway databases

BioCyciYEAST:YML115C-MONOMER.

Miscellaneous databases

PROiP23642.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiVAN1_YEAST
AccessioniPrimary (citable) accession number: P23642
Secondary accession number(s): D6W0G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4750 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.