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Protein

Mitochondrial phosphate carrier protein

Gene

MIR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transport of phosphate groups from the cytosol to the mitochondrial matrix.

GO - Molecular functioni

  • inorganic phosphate transmembrane transporter activity Source: SGD

GO - Biological processi

  • phosphate ion transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31707-MONOMER.

Protein family/group databases

TCDBi2.A.29.4.3. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial phosphate carrier protein
Alternative name(s):
Mitochondrial import receptor
Phosphate transport protein
Short name:
PTP
mPic 1
p32
Cleaved into the following chain:
Gene namesi
Name:MIR1
Ordered Locus Names:YJR077C
ORF Names:J1837
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR077C.
SGDiS000003838. MIR1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei20 – 4021Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini41 – 6222Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei63 – 8321Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini84 – 11532Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei116 – 13621Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini137 – 16529Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei166 – 18621Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini187 – 21428Mitochondrial intermembraneSequence analysisAdd
BLAST
Transmembranei215 – 23521Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini236 – 25318Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei254 – 27421Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini275 – 31137Mitochondrial intermembraneSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of mitochondrial inner membrane Source: SGD
  • mitochondrial inner membrane Source: Reactome
  • mitochondrial intermembrane space Source: Reactome
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 311311Mitochondrial phosphate carrier proteinPRO_0000090635Add
BLAST
Initiator methionineiRemoved; alternateCombined sources
Chaini2 – 311310Mitochondrial phosphate carrier protein, N-terminally processedPRO_0000423225Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine; in Mitochondrial phosphate carrier protein, N-terminally processedCombined sources
Modified residuei4 – 41PhosphoserineCombined sources
Modified residuei145 – 1451PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP23641.
PeptideAtlasiP23641.

PTM databases

iPTMnetiP23641.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TAT1P380853EBI-11178,EBI-20222

Protein-protein interaction databases

BioGridi33833. 72 interactions.
DIPiDIP-1714N.
IntActiP23641. 51 interactions.
MINTiMINT-393656.

Structurei

3D structure databases

ProteinModelPortaliP23641.
SMRiP23641. Positions 122-298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati15 – 10086Solcar 1Add
BLAST
Repeati114 – 19784Solcar 2Add
BLAST
Repeati212 – 29786Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00840000131664.
HOGENOMiHOG000164438.
InParanoidiP23641.
KOiK15102.
OMAiYTVTKFV.
OrthoDBiEOG7D2FQH.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23641-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSAAPAIP QYSVSDYMKF ALAGAIGCGS THSSMVPIDV VKTRIQLEPT
60 70 80 90 100
VYNKGMVGSF KQIIAGEGAG ALLTGFGPTL LGYSIQGAFK FGGYEVFKKF
110 120 130 140 150
FIDNLGYDTA SRYKNSVYMG SAAMAEFLAD IALCPLEATR IRLVSQPQFA
160 170 180 190 200
NGLVGGFSRI LKEEGIGSFY SGFTPILFKQ IPYNIAKFLV FERASEFYYG
210 220 230 240 250
FAGPKEKLSS TSTTLLNLLS GLTAGLAAAI VSQPADTLLS KVNKTKKAPG
260 270 280 290 300
QSTVGLLAQL AKQLGFFGSF AGLPTRLVMV GTLTSLQFGI YGSLKSTLGC
310
PPTIEIGGGG H
Length:311
Mass (Da):32,812
Last modified:November 1, 1991 - v1
Checksum:i89D1D97C49A9E8F1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21S → Y AA sequence (PubMed:2170848).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57478 Genomic DNA. Translation: CAA40716.1.
M54879 Genomic DNA. Translation: AAA34782.1.
Z49577 Genomic DNA. Translation: CAA89605.1.
L47993 Genomic DNA. Translation: AAB39302.1.
AY557913 Genomic DNA. Translation: AAS56239.1.
BK006943 Genomic DNA. Translation: DAA08863.1.
PIRiS12318.
RefSeqiNP_012611.1. NM_001181735.1.

Genome annotation databases

EnsemblFungiiYJR077C; YJR077C; YJR077C.
GeneIDi853540.
KEGGisce:YJR077C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57478 Genomic DNA. Translation: CAA40716.1.
M54879 Genomic DNA. Translation: AAA34782.1.
Z49577 Genomic DNA. Translation: CAA89605.1.
L47993 Genomic DNA. Translation: AAB39302.1.
AY557913 Genomic DNA. Translation: AAS56239.1.
BK006943 Genomic DNA. Translation: DAA08863.1.
PIRiS12318.
RefSeqiNP_012611.1. NM_001181735.1.

3D structure databases

ProteinModelPortaliP23641.
SMRiP23641. Positions 122-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33833. 72 interactions.
DIPiDIP-1714N.
IntActiP23641. 51 interactions.
MINTiMINT-393656.

Protein family/group databases

TCDBi2.A.29.4.3. the mitochondrial carrier (mc) family.

PTM databases

iPTMnetiP23641.

Proteomic databases

MaxQBiP23641.
PeptideAtlasiP23641.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR077C; YJR077C; YJR077C.
GeneIDi853540.
KEGGisce:YJR077C.

Organism-specific databases

EuPathDBiFungiDB:YJR077C.
SGDiS000003838. MIR1.

Phylogenomic databases

GeneTreeiENSGT00840000131664.
HOGENOMiHOG000164438.
InParanoidiP23641.
KOiK15102.
OMAiYTVTKFV.
OrthoDBiEOG7D2FQH.

Enzyme and pathway databases

BioCyciYEAST:G3O-31707-MONOMER.

Miscellaneous databases

NextBioi974254.
PROiP23641.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the gene for a yeast mitochondrial import receptor."
    Murakami H., Blobel G., Pain D.
    Nature 347:488-491(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-27.
  2. "Cloning and characterization of the mitochondrial phosphate transport protein gene from the yeast Saccharomyces cerevisiae."
    Phelps A., Schobert C.T., Wohlrab H.
    Biochemistry 30:248-252(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
    Huang M.-E., Manus V., Chuat J.-C., Galibert F.
    Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
    Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
    Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4 AND SER-145, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiMPCP_YEAST
AccessioniPrimary (citable) accession number: P23641
Secondary accession number(s): D6VWP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 11, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.