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P23640

- RB27A_RAT

UniProt

P23640 - RB27A_RAT

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Protein
Ras-related protein Rab-27A
Gene
Rab27a, Rab27
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 249GTP By similarity
Nucleotide bindingi74 – 785GTP By similarity
Nucleotide bindingi133 – 1364GTP By similarity
Nucleotide bindingi163 – 1653GTP By similarity

GO - Molecular functioni

  1. GDP binding Source: UniProtKB
  2. GTP binding Source: UniProtKB
  3. GTPase activity Source: UniProtKB
  4. protein binding Source: RGD
  5. protein domain specific binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. blood coagulation Source: Ensembl
  2. cytotoxic T cell degranulation Source: Ensembl
  3. exocytosis Source: RGD
  4. melanocyte differentiation Source: Ensembl
  5. melanosome transport Source: Ensembl
  6. multivesicular body sorting pathway Source: Ensembl
  7. natural killer cell degranulation Source: Ensembl
  8. positive regulation of exocytosis Source: Ensembl
  9. protein targeting Source: Ensembl
  10. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-27A
Short name:
Rab-27
Alternative name(s):
GTP-binding protein Ram
Ram p25
Gene namesi
Name:Rab27a
Synonyms:Rab27
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi620918. Rab27a.

Subcellular locationi

Membrane; Lipid-anchor. Melanosome By similarity. Late endosome By similarity. Lysosome By similarity
Note: Localizes to endosomal exocytic vesicles By similarity.

GO - Cellular componenti

  1. Golgi apparatus Source: Ensembl
  2. apical plasma membrane Source: RGD
  3. dendrite Source: Ensembl
  4. exocytic vesicle Source: Ensembl
  5. lysosome Source: UniProtKB-SubCell
  6. melanosome Source: UniProtKB-SubCell
  7. multivesicular body membrane Source: Ensembl
  8. photoreceptor outer segment Source: Ensembl
  9. secretory granule Source: RGD
  10. secretory granule membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi19 – 191G → V: No binding of GTP and GDP.
Mutagenesisi41 – 411T → S: Increased rates of GTP binding and GDP dissociation.
Mutagenesisi76 – 761A → T: Increased rates of GTP binding and GDP dissociation.
Mutagenesisi78 – 781Q → L: Increased GTP dissociation and decreased GDP hydrolysis.
Mutagenesisi133 – 1331N → H: Increased rates of GTP binding and GDP dissociation.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 221220Ras-related protein Rab-27A
PRO_0000121223Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Disulfide bondi123 ↔ 188 By similarity
Lipidationi219 – 2191S-geranylgeranyl cysteine By similarity
Modified residuei221 – 2211Cysteine methyl ester By similarity
Lipidationi221 – 2211S-geranylgeranyl cysteine By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiP23640.
PRIDEiP23640.

Expressioni

Tissue specificityi

High levels in eye, intestine, lung, pancreas and spleen, and low or absent in brain, liver, heart, kidney, and skeletal muscle.

Gene expression databases

GenevestigatoriP23640.

Interactioni

Subunit structurei

Binds SYTL1, SYTL2, SLAC2B, MYRIP, SYTL3, SYTL4 and SYTL5. Interacts with RPH3A and RPH3A. Binds MLPH. Interacts with UNC13D By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010012.

Structurei

3D structure databases

ProteinModelPortaliP23640.
SMRiP23640. Positions 5-190.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi38 – 469Effector region By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00740000114962.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP23640.
KOiK07885.
OMAiDQRAVKE.
OrthoDBiEOG7FFMSC.
PhylomeDBiP23640.
TreeFamiTF312895.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23640-1 [UniParc]FASTAAdd to Basket

« Hide

MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR    50
VVYRANGPDG TVGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD 100
LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RAVKEEEARE 150
LAEKYGIPYF ETSAANGTNI SQAIEMLLDL IMKRMERCVD KSWIPEGVVR 200
SNGHTSTDQL SEEKEKGLCG C 221
Length:221
Mass (Da):25,068
Last modified:November 1, 1991 - v1
Checksum:i6A3125DCC4738C80
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17352 mRNA. Translation: BAA04167.1.
BC098667 mRNA. Translation: AAH98667.1.
PIRiS12959.
RefSeqiNP_059013.1. NM_017317.2.
XP_006243404.1. XM_006243342.1.
UniGeneiRn.37360.

Genome annotation databases

EnsembliENSRNOT00000010012; ENSRNOP00000010012; ENSRNOG00000007533.
GeneIDi50645.
KEGGirno:50645.
UCSCiRGD:620918. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17352 mRNA. Translation: BAA04167.1 .
BC098667 mRNA. Translation: AAH98667.1 .
PIRi S12959.
RefSeqi NP_059013.1. NM_017317.2.
XP_006243404.1. XM_006243342.1.
UniGenei Rn.37360.

3D structure databases

ProteinModelPortali P23640.
SMRi P23640. Positions 5-190.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000010012.

Proteomic databases

PaxDbi P23640.
PRIDEi P23640.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000010012 ; ENSRNOP00000010012 ; ENSRNOG00000007533 .
GeneIDi 50645.
KEGGi rno:50645.
UCSCi RGD:620918. rat.

Organism-specific databases

CTDi 5873.
RGDi 620918. Rab27a.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00740000114962.
HOGENOMi HOG000233968.
HOVERGENi HBG009351.
InParanoidi P23640.
KOi K07885.
OMAi DQRAVKE.
OrthoDBi EOG7FFMSC.
PhylomeDBi P23640.
TreeFami TF312895.

Miscellaneous databases

NextBioi 610454.
PROi P23640.

Gene expression databases

Genevestigatori P23640.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ram: a novel low molecular weight GTP-binding protein cDNA from a rat megakaryocyte library."
    Nagata K., Satoh T., Itoh H., Kozasa T., Okano Y., Doi T., Kaziro Y., Nozawa Y.
    FEBS Lett. 275:29-32(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Megakaryocyte.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  3. "Characterization and site-directed mutagenesis of a low M(r) GTP-binding protein, ram p25, expressed in Escherichia coli."
    Nagata K., Suzuki T., Shibagaki Y., Mizumoto K., Okano Y., Kaziro Y., Nozawa Y.
    J. Biol. Chem. 267:19600-19606(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS.

Entry informationi

Entry nameiRB27A_RAT
AccessioniPrimary (citable) accession number: P23640
Secondary accession number(s): Q4KMA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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