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P23640

- RB27A_RAT

UniProt

P23640 - RB27A_RAT

Protein

Ras-related protein Rab-27A

Gene

Rab27a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 249GTPBy similarity
    Nucleotide bindingi74 – 785GTPBy similarity
    Nucleotide bindingi133 – 1364GTPBy similarity
    Nucleotide bindingi163 – 1653GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: UniProtKB
    2. GTPase activity Source: UniProtKB
    3. GTP binding Source: UniProtKB
    4. protein binding Source: RGD
    5. protein domain specific binding Source: UniProtKB

    GO - Biological processi

    1. blood coagulation Source: Ensembl
    2. cytotoxic T cell degranulation Source: Ensembl
    3. exocytosis Source: RGD
    4. melanocyte differentiation Source: Ensembl
    5. melanosome transport Source: Ensembl
    6. multivesicular body sorting pathway Source: Ensembl
    7. natural killer cell degranulation Source: Ensembl
    8. positive regulation of exocytosis Source: Ensembl
    9. protein targeting Source: Ensembl
    10. small GTPase mediated signal transduction Source: InterPro

    Keywords - Biological processi

    Exocytosis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-27A
    Short name:
    Rab-27
    Alternative name(s):
    GTP-binding protein Ram
    Ram p25
    Gene namesi
    Name:Rab27a
    Synonyms:Rab27
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi620918. Rab27a.

    Subcellular locationi

    Membrane; Lipid-anchor. Melanosome By similarity. Late endosome By similarity. Lysosome By similarity
    Note: Localizes to endosomal exocytic vesicles.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: RGD
    2. dendrite Source: Ensembl
    3. exocytic vesicle Source: Ensembl
    4. Golgi apparatus Source: Ensembl
    5. lysosome Source: UniProtKB-SubCell
    6. melanosome Source: UniProtKB-SubCell
    7. multivesicular body membrane Source: Ensembl
    8. photoreceptor outer segment Source: Ensembl
    9. secretory granule Source: RGD
    10. secretory granule membrane Source: RGD

    Keywords - Cellular componenti

    Endosome, Lysosome, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi19 – 191G → V: No binding of GTP and GDP. 1 Publication
    Mutagenesisi41 – 411T → S: Increased rates of GTP binding and GDP dissociation. 1 Publication
    Mutagenesisi76 – 761A → T: Increased rates of GTP binding and GDP dissociation. 1 Publication
    Mutagenesisi78 – 781Q → L: Increased GTP dissociation and decreased GDP hydrolysis. 1 Publication
    Mutagenesisi133 – 1331N → H: Increased rates of GTP binding and GDP dissociation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 221220Ras-related protein Rab-27APRO_0000121223Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Disulfide bondi123 ↔ 188By similarity
    Lipidationi219 – 2191S-geranylgeranyl cysteineBy similarity
    Modified residuei221 – 2211Cysteine methyl esterBy similarity
    Lipidationi221 – 2211S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Acetylation, Disulfide bond, Lipoprotein, Methylation, Prenylation

    Proteomic databases

    PaxDbiP23640.
    PRIDEiP23640.

    Expressioni

    Tissue specificityi

    High levels in eye, intestine, lung, pancreas and spleen, and low or absent in brain, liver, heart, kidney, and skeletal muscle.

    Gene expression databases

    GenevestigatoriP23640.

    Interactioni

    Subunit structurei

    Binds SYTL1, SYTL2, SLAC2B, MYRIP, SYTL3, SYTL4 and SYTL5. Interacts with RPH3A and RPH3A. Binds MLPH. Interacts with UNC13D By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000010012.

    Structurei

    3D structure databases

    ProteinModelPortaliP23640.
    SMRiP23640. Positions 5-190.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi38 – 469Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00740000114962.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiP23640.
    KOiK07885.
    OMAiDQRAVKE.
    OrthoDBiEOG7FFMSC.
    PhylomeDBiP23640.
    TreeFamiTF312895.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23640-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR    50
    VVYRANGPDG TVGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD 100
    LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RAVKEEEARE 150
    LAEKYGIPYF ETSAANGTNI SQAIEMLLDL IMKRMERCVD KSWIPEGVVR 200
    SNGHTSTDQL SEEKEKGLCG C 221
    Length:221
    Mass (Da):25,068
    Last modified:November 1, 1991 - v1
    Checksum:i6A3125DCC4738C80
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17352 mRNA. Translation: BAA04167.1.
    BC098667 mRNA. Translation: AAH98667.1.
    PIRiS12959.
    RefSeqiNP_059013.1. NM_017317.2.
    XP_006243404.1. XM_006243342.1.
    UniGeneiRn.37360.

    Genome annotation databases

    EnsembliENSRNOT00000010012; ENSRNOP00000010012; ENSRNOG00000007533.
    GeneIDi50645.
    KEGGirno:50645.
    UCSCiRGD:620918. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17352 mRNA. Translation: BAA04167.1 .
    BC098667 mRNA. Translation: AAH98667.1 .
    PIRi S12959.
    RefSeqi NP_059013.1. NM_017317.2.
    XP_006243404.1. XM_006243342.1.
    UniGenei Rn.37360.

    3D structure databases

    ProteinModelPortali P23640.
    SMRi P23640. Positions 5-190.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000010012.

    Proteomic databases

    PaxDbi P23640.
    PRIDEi P23640.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000010012 ; ENSRNOP00000010012 ; ENSRNOG00000007533 .
    GeneIDi 50645.
    KEGGi rno:50645.
    UCSCi RGD:620918. rat.

    Organism-specific databases

    CTDi 5873.
    RGDi 620918. Rab27a.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00740000114962.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi P23640.
    KOi K07885.
    OMAi DQRAVKE.
    OrthoDBi EOG7FFMSC.
    PhylomeDBi P23640.
    TreeFami TF312895.

    Miscellaneous databases

    NextBioi 610454.
    PROi P23640.

    Gene expression databases

    Genevestigatori P23640.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ram: a novel low molecular weight GTP-binding protein cDNA from a rat megakaryocyte library."
      Nagata K., Satoh T., Itoh H., Kozasa T., Okano Y., Doi T., Kaziro Y., Nozawa Y.
      FEBS Lett. 275:29-32(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Megakaryocyte.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.
    3. "Characterization and site-directed mutagenesis of a low M(r) GTP-binding protein, ram p25, expressed in Escherichia coli."
      Nagata K., Suzuki T., Shibagaki Y., Mizumoto K., Okano Y., Kaziro Y., Nozawa Y.
      J. Biol. Chem. 267:19600-19606(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, MUTAGENESIS.

    Entry informationi

    Entry nameiRB27A_RAT
    AccessioniPrimary (citable) accession number: P23640
    Secondary accession number(s): Q4KMA7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3