P23640 (RB27A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-27A
Short name=Rab-27

Alternative name(s):
GTP-binding protein Ram
Ram p25

Gene names

Name:	Rab27a
Synonyms:	Rab27

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse By similarity.
Subunit structure	Binds SYTL1, SYTL2, SLAC2B, MYRIP, SYTL3, SYTL4 and SYTL5. Interacts with RPH3A and RPH3A. Binds MLPH. Interacts with UNC13D By similarity.
Subcellular location	Membrane; Lipid-anchor. Melanosome By similarity. Late endosome By similarity. Lysosome By similarity. Note: Localizes to endosomal exocytic vesicles By similarity.
Tissue specificity	High levels in eye, intestine, lung, pancreas and spleen, and low or absent in brain, liver, heart, kidney, and skeletal muscle.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Exocytosis
Cellular component	Endosome Lysosome Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Disulfide bond Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Inferred from electronic annotation. Source: Compara cytotoxic T cell degranulation Inferred from electronic annotation. Source: Compara exocytosis Traceable author statement PubMed 17067543. Source: RGD melanocyte differentiation Inferred from electronic annotation. Source: Compara melanosome transport Inferred from electronic annotation. Source: Compara multivesicular body sorting pathway Inferred from electronic annotation. Source: Compara natural killer cell degranulation Inferred from electronic annotation. Source: Compara positive regulation of exocytosis Inferred from electronic annotation. Source: Compara protein targeting Inferred from electronic annotation. Source: Compara small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara apical plasma membrane Inferred from direct assay PubMed 17067543. Source: RGD dendrite Inferred from electronic annotation. Source: Compara exocytic vesicle Inferred from electronic annotation. Source: Compara lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell multivesicular body membrane Inferred from electronic annotation. Source: Compara photoreceptor outer segment Inferred from electronic annotation. Source: Compara secretory granule membrane Inferred from direct assay PubMed 17067543. Source: RGD
Molecular_function	GDP binding Inferred from sequence or structural similarity. Source: UniProtKB GTP binding Inferred from sequence or structural similarity. Source: UniProtKB GTPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Ras-related protein Rab-27A		PRO_0000121223
Regions
	Nucleotide binding	16 – 24	9	GTP By similarity
	Nucleotide binding	74 – 78	5	GTP By similarity
	Nucleotide binding	133 – 136	4	GTP By similarity
	Nucleotide binding	163 – 165	3	GTP By similarity
	Motif	38 – 46	9	Effector region By similarity
Amino acid modifications
	Modified residue	211	1	Phosphoserine By similarity
	Modified residue	221	1	Cysteine methyl ester By similarity
	Lipidation	219	1	S-geranylgeranyl cysteine By similarity
	Lipidation	221	1	S-geranylgeranyl cysteine By similarity
	Disulfide bond	123 ↔ 188		By similarity
Experimental info
	Mutagenesis	19	1	G → V: No binding of GTP and GDP.
	Mutagenesis	41	1	T → S: Increased rates of GTP binding and GDP dissociation.
	Mutagenesis	76	1	A → T: Increased rates of GTP binding and GDP dissociation.
	Mutagenesis	78	1	Q → L: Increased GTP dissociation and decreased GDP hydrolysis.
	Mutagenesis	133	1	N → H: Increased rates of GTP binding and GDP dissociation.

Sequences

	Sequence		Length	Mass (Da)
	P23640 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 6A3125DCC4738C80	FASTA	221	25,068
	10 20 30 40 50 60 MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR VVYRANGPDG 70 80 90 100 110 120 TVGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD LTNEQSFLNV RNWISQLQMH 130 140 150 160 170 180 AYCENPDIVL CGNKSDLEDQ RAVKEEEARE LAEKYGIPYF ETSAANGTNI SQAIEMLLDL 190 200 210 220 IMKRMERCVD KSWIPEGVVR SNGHTSTDQL SEEKEKGLCG C

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ram: a novel low molecular weight GTP-binding protein cDNA from a rat megakaryocyte library." Nagata K., Satoh T., Itoh H., Kozasa T., Okano Y., Doi T., Kaziro Y., Nozawa Y. FEBS Lett. 275:29-32(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Megakaryocyte.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
[3]	"Characterization and site-directed mutagenesis of a low M(r) GTP-binding protein, ram p25, expressed in Escherichia coli." Nagata K., Suzuki T., Shibagaki Y., Mizumoto K., Okano Y., Kaziro Y., Nozawa Y. J. Biol. Chem. 267:19600-19606(1992) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17352 mRNA. Translation: BAA04167.1. BC098667 mRNA. Translation: AAH98667.1.
IPI	IPI00212794.
PIR	S12959.
RefSeq	NP_059013.1. NM_017317.2.
UniGene	Rn.37360.
3D structure databases
ProteinModelPortal	P23640.
SMR	P23640. Positions 5-190.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000010012.
Proteomic databases
PaxDb	P23640.
PRIDE	P23640.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000010012; ENSRNOP00000010012; ENSRNOG00000007533.
GeneID	50645.
KEGG	rno:50645.
UCSC	RGD:620918. rat.
Organism-specific databases
CTD	5873.
RGD	620918. Rab27a.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00700000104288.
HOGENOM	HOG000233968.
HOVERGEN	HBG009351.
InParanoid	P23640.
KO	K07885.
OMA	DQRAVKE.
OrthoDB	EOG4PRSRM.
Gene expression databases
Genevestigator	P23640.
GermOnline	ENSRNOG00000007533. Rattus norvegicus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	610454.

Entry information

Entry name

RB27A_RAT

Accession

Primary (citable) accession number: P23640
Secondary accession number(s): Q4KMA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program