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Protein

Ras-related protein Rab-27A

Gene

Rab27a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 249GTPBy similarity
Nucleotide bindingi74 – 785GTPBy similarity
Nucleotide bindingi133 – 1364GTPBy similarity
Nucleotide bindingi163 – 1653GTPBy similarity

GO - Molecular functioni

  1. GDP binding Source: UniProtKB
  2. GTPase activity Source: UniProtKB
  3. GTP binding Source: UniProtKB
  4. protein domain specific binding Source: UniProtKB

GO - Biological processi

  1. antigen processing and presentation Source: Ensembl
  2. blood coagulation Source: Ensembl
  3. cytotoxic T cell degranulation Source: Ensembl
  4. exocytosis Source: RGD
  5. exosomal secretion Source: Ensembl
  6. intracellular protein transport Source: GO_Central
  7. melanocyte differentiation Source: Ensembl
  8. melanosome transport Source: GO_Central
  9. metabolic process Source: GOC
  10. multivesicular body organization Source: Ensembl
  11. multivesicular body sorting pathway Source: Ensembl
  12. natural killer cell degranulation Source: Ensembl
  13. positive regulation of constitutive secretory pathway Source: Ensembl
  14. positive regulation of exocytosis Source: GO_Central
  15. positive regulation of gene expression Source: Ensembl
  16. positive regulation of regulated secretory pathway Source: Ensembl
  17. protein secretion Source: GO_Central
  18. protein targeting Source: Ensembl
  19. Rab protein signal transduction Source: GO_Central
  20. vesicle docking involved in exocytosis Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-27A
Short name:
Rab-27
Alternative name(s):
GTP-binding protein Ram
Ram p25
Gene namesi
Name:Rab27a
Synonyms:Rab27
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi620918. Rab27a.

Subcellular locationi

  1. Membrane; Lipid-anchor
  2. Melanosome By similarity
  3. Late endosome By similarity
  4. Lysosome By similarity

  5. Note: Localizes to endosomal exocytic vesicles.By similarity

GO - Cellular componenti

  1. apical plasma membrane Source: RGD
  2. dendrite Source: Ensembl
  3. exocytic vesicle Source: Ensembl
  4. extracellular vesicular exosome Source: Ensembl
  5. Golgi apparatus Source: Ensembl
  6. lysosome Source: UniProtKB-SubCell
  7. melanosome Source: GO_Central
  8. multivesicular body membrane Source: Ensembl
  9. photoreceptor outer segment Source: Ensembl
  10. secretory granule Source: RGD
  11. secretory granule membrane Source: RGD
  12. Weibel-Palade body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi19 – 191G → V: No binding of GTP and GDP. 1 Publication
Mutagenesisi41 – 411T → S: Increased rates of GTP binding and GDP dissociation. 1 Publication
Mutagenesisi76 – 761A → T: Increased rates of GTP binding and GDP dissociation. 1 Publication
Mutagenesisi78 – 781Q → L: Increased GTP dissociation and decreased GDP hydrolysis. 1 Publication
Mutagenesisi133 – 1331N → H: Increased rates of GTP binding and GDP dissociation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 221220Ras-related protein Rab-27APRO_0000121223Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Disulfide bondi123 ↔ 188By similarity
Lipidationi219 – 2191S-geranylgeranyl cysteineBy similarity
Modified residuei221 – 2211Cysteine methyl esterBy similarity
Lipidationi221 – 2211S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Acetylation, Disulfide bond, Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiP23640.
PRIDEiP23640.

Expressioni

Tissue specificityi

High levels in eye, intestine, lung, pancreas and spleen, and low or absent in brain, liver, heart, kidney, and skeletal muscle.

Gene expression databases

GenevestigatoriP23640.

Interactioni

Subunit structurei

Binds SYTL1, SYTL2, SLAC2B, MYRIP, SYTL3, SYTL4 and SYTL5. Interacts with RPH3A and RPH3A. Binds MLPH. Interacts with UNC13D (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010012.

Structurei

3D structure databases

SMRiP23640. Positions 5-190.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi38 – 469Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00770000120510.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP23640.
KOiK07885.
OMAiKEKGACG.
OrthoDBiEOG7FFMSC.
PhylomeDBiP23640.
TreeFamiTF312895.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23640-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR
60 70 80 90 100
VVYRANGPDG TVGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD
110 120 130 140 150
LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RAVKEEEARE
160 170 180 190 200
LAEKYGIPYF ETSAANGTNI SQAIEMLLDL IMKRMERCVD KSWIPEGVVR
210 220
SNGHTSTDQL SEEKEKGLCG C
Length:221
Mass (Da):25,068
Last modified:November 1, 1991 - v1
Checksum:i6A3125DCC4738C80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17352 mRNA. Translation: BAA04167.1.
BC098667 mRNA. Translation: AAH98667.1.
PIRiS12959.
RefSeqiNP_059013.1. NM_017317.2.
UniGeneiRn.37360.

Genome annotation databases

EnsembliENSRNOT00000010012; ENSRNOP00000010012; ENSRNOG00000007533.
GeneIDi50645.
KEGGirno:50645.
UCSCiRGD:620918. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17352 mRNA. Translation: BAA04167.1.
BC098667 mRNA. Translation: AAH98667.1.
PIRiS12959.
RefSeqiNP_059013.1. NM_017317.2.
UniGeneiRn.37360.

3D structure databases

SMRiP23640. Positions 5-190.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010012.

Proteomic databases

PaxDbiP23640.
PRIDEiP23640.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010012; ENSRNOP00000010012; ENSRNOG00000007533.
GeneIDi50645.
KEGGirno:50645.
UCSCiRGD:620918. rat.

Organism-specific databases

CTDi5873.
RGDi620918. Rab27a.

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00770000120510.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP23640.
KOiK07885.
OMAiKEKGACG.
OrthoDBiEOG7FFMSC.
PhylomeDBiP23640.
TreeFamiTF312895.

Miscellaneous databases

NextBioi610454.
PROiP23640.

Gene expression databases

GenevestigatoriP23640.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The ram: a novel low molecular weight GTP-binding protein cDNA from a rat megakaryocyte library."
    Nagata K., Satoh T., Itoh H., Kozasa T., Okano Y., Doi T., Kaziro Y., Nozawa Y.
    FEBS Lett. 275:29-32(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Megakaryocyte.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  3. "Characterization and site-directed mutagenesis of a low M(r) GTP-binding protein, ram p25, expressed in Escherichia coli."
    Nagata K., Suzuki T., Shibagaki Y., Mizumoto K., Okano Y., Kaziro Y., Nozawa Y.
    J. Biol. Chem. 267:19600-19606(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS.

Entry informationi

Entry nameiRB27A_RAT
AccessioniPrimary (citable) accession number: P23640
Secondary accession number(s): Q4KMA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 1, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.