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Protein

Ras-related protein Rab-27A

Gene

Rab27a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 24GTPBy similarity9
Nucleotide bindingi74 – 78GTPBy similarity5
Nucleotide bindingi133 – 136GTPBy similarity4
Nucleotide bindingi163 – 165GTPBy similarity3

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • myosin V binding Source: Ensembl
  • protein domain specific binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processExocytosis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-6798695. Neutrophil degranulation.
R-RNO-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-27A
Short name:
Rab-27
Alternative name(s):
GTP-binding protein Ram
Ram p25
Gene namesi
Name:Rab27a
Synonyms:Rab27
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi620918. Rab27a.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19G → V: No binding of GTP and GDP. 1 Publication1
Mutagenesisi41T → S: Increased rates of GTP binding and GDP dissociation. 1 Publication1
Mutagenesisi76A → T: Increased rates of GTP binding and GDP dissociation. 1 Publication1
Mutagenesisi78Q → L: Increased GTP dissociation and decreased GDP hydrolysis. 1 Publication1
Mutagenesisi133N → H: Increased rates of GTP binding and GDP dissociation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001212232 – 221Ras-related protein Rab-27AAdd BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Disulfide bondi123 ↔ 188By similarity
Lipidationi219S-geranylgeranyl cysteineBy similarity1
Modified residuei221Cysteine methyl esterBy similarity1
Lipidationi221S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP23640.
PRIDEiP23640.

PTM databases

PhosphoSitePlusiP23640.

Expressioni

Tissue specificityi

High levels in eye, intestine, lung, pancreas and spleen, and low or absent in brain, liver, heart, kidney, and skeletal muscle.

Gene expression databases

BgeeiENSRNOG00000052499.
GenevisibleiP23640. RN.

Interactioni

Subunit structurei

Binds SYTL1, SYTL2, SLAC2B, MYRIP, SYTL3, SYTL4 and SYTL5. Interacts with RPH3A and RPH3A. Binds MLPH. Interacts with UNC13D. Does not interact with the BLOC-3 complex (heterodimer of HPS1 and HPS4) (By similarity).By similarity

GO - Molecular functioni

  • myosin V binding Source: Ensembl
  • protein domain specific binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010012.

Structurei

3D structure databases

ProteinModelPortaliP23640.
SMRiP23640.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi38 – 46Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0081. Eukaryota.
ENOG410XQ3T. LUCA.
GeneTreeiENSGT00890000139330.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP23640.
KOiK07885.
OMAiLVMQRIQ.
OrthoDBiEOG091G0I51.
PhylomeDBiP23640.
TreeFamiTF312895.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51419. RAB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23640-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR
60 70 80 90 100
VVYRANGPDG TVGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD
110 120 130 140 150
LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RAVKEEEARE
160 170 180 190 200
LAEKYGIPYF ETSAANGTNI SQAIEMLLDL IMKRMERCVD KSWIPEGVVR
210 220
SNGHTSTDQL SEEKEKGLCG C
Length:221
Mass (Da):25,068
Last modified:November 1, 1991 - v1
Checksum:i6A3125DCC4738C80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17352 mRNA. Translation: BAA04167.1.
BC098667 mRNA. Translation: AAH98667.1.
PIRiS12959.
RefSeqiNP_059013.1. NM_017317.2.
XP_017451353.1. XM_017595864.1.
UniGeneiRn.37360.

Genome annotation databases

EnsembliENSRNOT00000083957; ENSRNOP00000068946; ENSRNOG00000052499.
GeneIDi50645.
KEGGirno:50645.
UCSCiRGD:620918. rat.

Similar proteinsi

Entry informationi

Entry nameiRB27A_RAT
AccessioniPrimary (citable) accession number: P23640
Secondary accession number(s): Q4KMA7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 25, 2017
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families