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P23640 (RB27A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-27A

Short name=Rab-27
Alternative name(s):
GTP-binding protein Ram
Ram p25
Gene names
Name:Rab27a
Synonyms:Rab27
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length221 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse By similarity.

Subunit structure

Binds SYTL1, SYTL2, SLAC2B, MYRIP, SYTL3, SYTL4 and SYTL5. Interacts with RPH3A and RPH3A. Binds MLPH. Interacts with UNC13D By similarity.

Subcellular location

Membrane; Lipid-anchor. Melanosome By similarity. Late endosome By similarity. Lysosome By similarity. Note: Localizes to endosomal exocytic vesicles By similarity.

Tissue specificity

High levels in eye, intestine, lung, pancreas and spleen, and low or absent in brain, liver, heart, kidney, and skeletal muscle.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
   Biological processExocytosis
   Cellular componentEndosome
Lysosome
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMAcetylation
Disulfide bond
Lipoprotein
Methylation
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processblood coagulation

Inferred from electronic annotation. Source: Ensembl

cytotoxic T cell degranulation

Inferred from electronic annotation. Source: Ensembl

exocytosis

Traceable author statement PubMed 17067543. Source: RGD

melanocyte differentiation

Inferred from electronic annotation. Source: Ensembl

melanosome transport

Inferred from electronic annotation. Source: Ensembl

multivesicular body sorting pathway

Inferred from electronic annotation. Source: Ensembl

natural killer cell degranulation

Inferred from electronic annotation. Source: Ensembl

positive regulation of exocytosis

Inferred from electronic annotation. Source: Ensembl

protein targeting

Inferred from electronic annotation. Source: Ensembl

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Ensembl

apical plasma membrane

Inferred from direct assay PubMed 17067543. Source: RGD

dendrite

Inferred from electronic annotation. Source: Ensembl

exocytic vesicle

Inferred from electronic annotation. Source: Ensembl

lysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

multivesicular body membrane

Inferred from electronic annotation. Source: Ensembl

photoreceptor outer segment

Inferred from electronic annotation. Source: Ensembl

secretory granule

Inferred from direct assay PubMed 15465017. Source: RGD

secretory granule membrane

Inferred from direct assay PubMed 17067543. Source: RGD

   Molecular_functionGDP binding

Inferred from sequence or structural similarity. Source: UniProtKB

GTP binding

Inferred from sequence or structural similarity. Source: UniProtKB

GTPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein domain specific binding

Inferred from physical interaction PubMed 16880209. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 221220Ras-related protein Rab-27A
PRO_0000121223

Regions

Nucleotide binding16 – 249GTP By similarity
Nucleotide binding74 – 785GTP By similarity
Nucleotide binding133 – 1364GTP By similarity
Nucleotide binding163 – 1653GTP By similarity
Motif38 – 469Effector region By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue2211Cysteine methyl ester By similarity
Lipidation2191S-geranylgeranyl cysteine By similarity
Lipidation2211S-geranylgeranyl cysteine By similarity
Disulfide bond123 ↔ 188 By similarity

Experimental info

Mutagenesis191G → V: No binding of GTP and GDP.
Mutagenesis411T → S: Increased rates of GTP binding and GDP dissociation.
Mutagenesis761A → T: Increased rates of GTP binding and GDP dissociation.
Mutagenesis781Q → L: Increased GTP dissociation and decreased GDP hydrolysis.
Mutagenesis1331N → H: Increased rates of GTP binding and GDP dissociation.

Sequences

Sequence LengthMass (Da)Tools
P23640 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 6A3125DCC4738C80

FASTA22125,068
        10         20         30         40         50         60 
MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR VVYRANGPDG 

        70         80         90        100        110        120 
TVGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD LTNEQSFLNV RNWISQLQMH 

       130        140        150        160        170        180 
AYCENPDIVL CGNKSDLEDQ RAVKEEEARE LAEKYGIPYF ETSAANGTNI SQAIEMLLDL 

       190        200        210        220 
IMKRMERCVD KSWIPEGVVR SNGHTSTDQL SEEKEKGLCG C 

« Hide

References

« Hide 'large scale' references
[1]"The ram: a novel low molecular weight GTP-binding protein cDNA from a rat megakaryocyte library."
Nagata K., Satoh T., Itoh H., Kozasa T., Okano Y., Doi T., Kaziro Y., Nozawa Y.
FEBS Lett. 275:29-32(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Megakaryocyte.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thymus.
[3]"Characterization and site-directed mutagenesis of a low M(r) GTP-binding protein, ram p25, expressed in Escherichia coli."
Nagata K., Suzuki T., Shibagaki Y., Mizumoto K., Okano Y., Kaziro Y., Nozawa Y.
J. Biol. Chem. 267:19600-19606(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, MUTAGENESIS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D17352 mRNA. Translation: BAA04167.1.
BC098667 mRNA. Translation: AAH98667.1.
PIRS12959.
RefSeqNP_059013.1. NM_017317.2.
XP_006243404.1. XM_006243342.1.
UniGeneRn.37360.

3D structure databases

ProteinModelPortalP23640.
SMRP23640. Positions 5-190.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000010012.

Proteomic databases

PaxDbP23640.
PRIDEP23640.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000010012; ENSRNOP00000010012; ENSRNOG00000007533.
GeneID50645.
KEGGrno:50645.
UCSCRGD:620918. rat.

Organism-specific databases

CTD5873.
RGD620918. Rab27a.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00740000114962.
HOGENOMHOG000233968.
HOVERGENHBG009351.
InParanoidP23640.
KOK07885.
OMADQRAVKE.
OrthoDBEOG7FFMSC.
PhylomeDBP23640.
TreeFamTF312895.

Gene expression databases

GenevestigatorP23640.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio610454.
PROP23640.

Entry information

Entry nameRB27A_RAT
AccessionPrimary (citable) accession number: P23640
Secondary accession number(s): Q4KMA7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families