Reviewed, UniProtKB/Swiss-Prot P23634 (AT2B4_HUMAN)

Names and origin

Protein names

Recommended name:
    Plasma membrane calcium-transporting ATPase 4
      Short name=PMCA4
    EC=3.6.3.8
Alternative name(s):
    Plasma membrane calcium ATPase isoform 4
    Plasma membrane calcium pump isoform 4
    Matrix-remodeling-associated protein 1

Gene names

Name:	ATP2B4
Synonyms:	ATP2B2, MXRA1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1241 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
Catalytic activity	ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Isoform XB is the most abundant isoform and is expressed ubiquitously. Isoforms containing segment Z have only been detected in heart, while isoforms containing segment a have been found in heart, stomach and brain cortex.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIB subfamily.

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Ligand	ATP-binding Calcium Calmodulin-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro calcium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activity Ref.1 Traceable author statement. Source: ProtInc calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Dlg1	Q62696	1	EBI-1174437,EBI-389325	From a different organism.
Dlg2	Q63622	2	EBI-1174437,EBI-396947	From a different organism.
Dlg3	Q62936	2	EBI-1174437,EBI-349596	From a different organism.
Dlg4	P31016	1	EBI-1174437,EBI-375655	From a different organism.

Alternative products

This entry describes 8 isoforms produced by alternative splicing. [Align] [Select]

Note: There is a combination of two alternatively spliced domains at N-terminal site A (X and Z) and at C-terminal site B/C (A, B, D and K). The splice sites have mostly been studied independently. Full isoforms so far detected are isoform XA and isoform XB. Experimental confirmation may be lacking for some isoforms.

Isoform XD (identifier: P23634-1)

Also known as: AIICIV;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform XA (identifier: P23634-2)

Also known as: AIICII;

The sequence of this isoform differs from the canonical sequence as follows:
     1140-1241: IKVVKAFHSS...SSLQSLETSV → VAVAPVKSSPTTSVPAVSSPPMGNQSGQSVP

Isoform ZA (identifier: P23634-3)

Also known as: AICII;

The sequence of this isoform differs from the canonical sequence as follows:
     301-312: Missing.
     1140-1241: IKVVKAFHSS...SSLQSLETSV → VAVAPVKSSPTTSVPAVSSPPMGNQSGQSVP

Isoform XK (identifier: P23634-4)

Also known as: XG;

The sequence of this isoform differs from the canonical sequence as follows:
     1009-1044: Missing.
     1140-1241: IKVVKAFHSS...SSLQSLETSV → VAVAPVKSSPTTSVPAVSSPPMGNQSGQSVP

Isoform ZK (identifier: P23634-5)

Also known as: ZG;

The sequence of this isoform differs from the canonical sequence as follows:
     301-312: Missing.
     1009-1044: Missing.
     1140-1241: IKVVKAFHSS...SSLQSLETSV → VAVAPVKSSPTTSVPAVSSPPMGNQSGQSVP

Isoform XB (identifier: P23634-6)

Also known as: AIICI;

The sequence of this isoform differs from the canonical sequence as follows:
     1104-1139: Missing.

Isoform ZB (identifier: P23634-7)

Also known as: AICI;

The sequence of this isoform differs from the canonical sequence as follows:
     301-312: Missing.
     1104-1139: Missing.

Isoform ZD (identifier: P23634-8)

Also known as: AICIV;

The sequence of this isoform differs from the canonical sequence as follows:
     301-312: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1241

1241

Plasma membrane calcium-transporting ATPase 4

PRO_0000046220

Regions

Topological domain

1 – 92

92

Cytoplasmic Potential

Transmembrane

93 – 113

21

Potential

Topological domain

114 – 150

37

Extracellular Potential

Transmembrane

151 – 171

21

Potential

Topological domain

172 – 356

185

Cytoplasmic Potential

Transmembrane

357 – 376

20

Potential

Topological domain

377 – 409

33

Extracellular Potential

Transmembrane

410 – 427

18

Potential

Topological domain

428 – 840

413

Cytoplasmic Potential

Transmembrane

841 – 860

20

Potential

Topological domain

861 – 870

10

Extracellular Potential

Transmembrane

871 – 891

21

Potential

Topological domain

892 – 911

20

Cytoplasmic Potential

Transmembrane

912 – 934

23

Potential

Topological domain

935 – 952

18

Extracellular Potential

Transmembrane

953 – 974

22

Potential

Topological domain

975 – 993

19

Cytoplasmic Potential

Transmembrane

994 – 1015

22

Potential

Topological domain

1016 – 1025

10

Extracellular Potential

Transmembrane

1026 – 1047

22

Potential

Topological domain

1048 – 1241

194

Cytoplasmic Potential

Region

1086 – 1103

18

Calmodulin-binding subdomain A

Region

1104 – 1113

10

Calmodulin-binding subdomain B By similarity

Compositional bias

297 – 303

7

Poly-Lys

Compositional bias

1186 – 1190

5

Poly-Glu

Sites

Active site

465

1

4-aspartylphosphate intermediate By similarity

Metal binding

785

1

Magnesium By similarity

Metal binding

789

1

Magnesium By similarity

Amino acid modifications

Modified residue

1102

1

Phosphothreonine; by PKC By similarity

Natural variations

Alternative sequence

301 – 312

12

Missing in isoform ZA, isoform ZK, isoform ZB and isoform ZD.

VSP_000402

Alternative sequence

1009 – 1044

36

Missing in isoform XK and isoform ZK.

VSP_000403

Alternative sequence

1104 – 1139

36

Missing in isoform XB and isoform ZB.

VSP_000404

Alternative sequence

1140 – 1241

102

IKVVK…LETSV → VAVAPVKSSPTTSVPAVSSP PMGNQSGQSVP in isoform XA, isoform XK, isoform ZA and isoform ZK.

VSP_000405

Experimental info

Sequence conflict

1144

1

K → N AA sequence Ref.6

Sequence conflict

1147

1

H → S AA sequence Ref.6

Sequence conflict

1153

1

S → F AA sequence Ref.6

Sequence conflict

1178

1

L → Q AA sequence Ref.7

Sequence conflict

1187

1

E → Q AA sequence Ref.7

Secondary structure

1

.

.

.

.

1241

Helix Strand Turn

Details...

Turn

1090 – 1092

3

Helix

1093 – 1102

10

Sequences

	Sequence		Length	Mass (Da)
	Isoform XD (AIICIV) [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 568544103CD5F494	FASTA	1,241	137,920
	10 20 30 40 50 60 MTNPSDRVLP ANSMAESREG DFGCTVMELR KLMELRSRDA LTQINVHYGG VQNLCSRLKT 70 80 90 100 110 120 SPVEGLSGNP ADLEKRRQVF GHNVIPPKKP KTFLELVWEA LQDVTLIILE IAAIISLVLS 130 140 150 160 170 180 FYRPAGEENE LCGQVATTPE DENEAQAGWI EGAAILFSVI IVVLVTAFND WSKEKQFRGL 190 200 210 220 230 240 QCRIEQEQKF SIIRNGQLIQ LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT 250 260 270 280 290 300 GESDHVKKSL DKDPMLLSGT HVMEGSGRMV VTAVGVNSQT GIILTLLGVN EDDEGEKKKK 310 320 330 340 350 360 GKKQGVPENR NKAKTQDGVA LEIQPLNSQE GIDNEEKDKK AVKVPKKEKS VLQGKLTRLA 370 380 390 400 410 420 VQIGKAGLLM SALTVFILIL YFVIDNFVIN RRPWLPECTP IYIQYFVKFF IIGITVLVVA 430 440 450 460 470 480 VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL DACETMGNAT AICSDKTGTL TMNRMTVVQA 490 500 510 520 530 540 YIGGIHYRQI PSPDVFLPKV LDLIVNGISI NSAYTSKILP PEKEGGLPRQ VGNKTECALL 550 560 570 580 590 600 GFVTDLKQDY QAVRNEVPEE KLYKVYTFNS VRKSMSTVIR NPNGGFRMYS KGASEIILRK 610 620 630 640 650 660 CNRILDRKGE AVPFKNKDRD DMVRTVIEPM ACDGLRTICI AYRDFDDTEP SWDNENEILT 670 680 690 700 710 720 ELTCIAVVGI EDPVRPEVPD AIAKCKQAGI TVRMVTGDNI NTARAIATKC GILTPGDDFL 730 740 750 760 770 780 CLEGKEFNRL IRNEKGEVEQ EKLDKIWPKL RVLARSSPTD KHTLVKGIID STVGEHRQVV 790 800 810 820 830 840 AVTGDGTNDG PALKKADVGF AMGIAGTDVA KEASDIILTD DNFTSIVKAV MWGRNVYDSI 850 860 870 880 890 900 SKFLQFQLTV NVVAVIVAFT GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTESLL 910 920 930 940 950 960 KRRPYGRNKP LISRTMMKNI LGHAFYQLIV IFILVFAGEK FFDIDSGRKA PLHSPPSQHY 970 980 990 1000 1010 1020 TIVFNTFVLM QLFNEINSRK IHGEKNVFSG IYRNIIFCSV VLGTFICQIF IVEFGGKPFS 1030 1040 1050 1060 1070 1080 CTSLSLSQWL WCLFIGIGEL LWGQFISAIP TRSLKFLKEA GHGTTKEEIT KDAEGLDEID 1090 1100 1110 1120 1130 1140 HAEMELRRGQ ILWFRGLNRI QTQIDVINTF QTGASFKGVL RRQNMGQHLD VKLVPSSSYI 1150 1160 1170 1180 1190 1200 KVVKAFHSSL HESIQKPYNQ KSIHSFMTHP EFAIEEELPR TPLLDEEEEE NPDKASKFGT 1210 1220 1230 1240 RVLLLDGEVT PYANTNNNAV DCNQVQLPQS DSSLQSLETS V
	Isoform XA (AIICII). Checksum: C4ED2E2EFE1BB043	FASTA	1,170	129,403
	10 20 30 40 50 60 MTNPSDRVLP ANSMAESREG DFGCTVMELR KLMELRSRDA LTQINVHYGG VQNLCSRLKT 70 80 90 100 110 120 SPVEGLSGNP ADLEKRRQVF GHNVIPPKKP KTFLELVWEA LQDVTLIILE IAAIISLVLS 130 140 150 160 170 180 FYRPAGEENE LCGQVATTPE DENEAQAGWI EGAAILFSVI IVVLVTAFND WSKEKQFRGL 190 200 210 220 230 240 QCRIEQEQKF SIIRNGQLIQ LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT 250 260 270 280 290 300 GESDHVKKSL DKDPMLLSGT HVMEGSGRMV VTAVGVNSQT GIILTLLGVN EDDEGEKKKK 310 320 330 340 350 360 GKKQGVPENR NKAKTQDGVA LEIQPLNSQE GIDNEEKDKK AVKVPKKEKS VLQGKLTRLA 370 380 390 400 410 420 VQIGKAGLLM SALTVFILIL YFVIDNFVIN RRPWLPECTP IYIQYFVKFF IIGITVLVVA 430 440 450 460 470 480 VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL DACETMGNAT AICSDKTGTL TMNRMTVVQA 490 500 510 520 530 540 YIGGIHYRQI PSPDVFLPKV LDLIVNGISI NSAYTSKILP PEKEGGLPRQ VGNKTECALL 550 560 570 580 590 600 GFVTDLKQDY QAVRNEVPEE KLYKVYTFNS VRKSMSTVIR NPNGGFRMYS KGASEIILRK 610 620 630 640 650 660 CNRILDRKGE AVPFKNKDRD DMVRTVIEPM ACDGLRTICI AYRDFDDTEP SWDNENEILT 670 680 690 700 710 720 ELTCIAVVGI EDPVRPEVPD AIAKCKQAGI TVRMVTGDNI NTARAIATKC GILTPGDDFL 730 740 750 760 770 780 CLEGKEFNRL IRNEKGEVEQ EKLDKIWPKL RVLARSSPTD KHTLVKGIID STVGEHRQVV 790 800 810 820 830 840 AVTGDGTNDG PALKKADVGF AMGIAGTDVA KEASDIILTD DNFTSIVKAV MWGRNVYDSI 850 860 870 880 890 900 SKFLQFQLTV NVVAVIVAFT GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTESLL 910 920 930 940 950 960 KRRPYGRNKP LISRTMMKNI LGHAFYQLIV IFILVFAGEK FFDIDSGRKA PLHSPPSQHY 970 980 990 1000 1010 1020 TIVFNTFVLM QLFNEINSRK IHGEKNVFSG IYRNIIFCSV VLGTFICQIF IVEFGGKPFS 1030 1040 1050 1060 1070 1080 CTSLSLSQWL WCLFIGIGEL LWGQFISAIP TRSLKFLKEA GHGTTKEEIT KDAEGLDEID 1090 1100 1110 1120 1130 1140 HAEMELRRGQ ILWFRGLNRI QTQIDVINTF QTGASFKGVL RRQNMGQHLD VKLVPSSSYV 1150 1160 1170 AVAPVKSSPT TSVPAVSSPP MGNQSGQSVP
	Isoform ZA (AICII). Checksum: E7AA8CC9C25E0727	FASTA	1,158	128,066
	10 20 30 40 50 60 MTNPSDRVLP ANSMAESREG DFGCTVMELR KLMELRSRDA LTQINVHYGG VQNLCSRLKT 70 80 90 100 110 120 SPVEGLSGNP ADLEKRRQVF GHNVIPPKKP KTFLELVWEA LQDVTLIILE IAAIISLVLS 130 140 150 160 170 180 FYRPAGEENE LCGQVATTPE DENEAQAGWI EGAAILFSVI IVVLVTAFND WSKEKQFRGL 190 200 210 220 230 240 QCRIEQEQKF SIIRNGQLIQ LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT 250 260 270 280 290 300 GESDHVKKSL DKDPMLLSGT HVMEGSGRMV VTAVGVNSQT GIILTLLGVN EDDEGEKKKK 310 320 330 340 350 360 AKTQDGVALE IQPLNSQEGI DNEEKDKKAV KVPKKEKSVL QGKLTRLAVQ IGKAGLLMSA 370 380 390 400 410 420 LTVFILILYF VIDNFVINRR PWLPECTPIY IQYFVKFFII GITVLVVAVP EGLPLAVTIS 430 440 450 460 470 480 LAYSVKKMMK DNNLVRHLDA CETMGNATAI CSDKTGTLTM NRMTVVQAYI GGIHYRQIPS 490 500 510 520 530 540 PDVFLPKVLD LIVNGISINS AYTSKILPPE KEGGLPRQVG NKTECALLGF VTDLKQDYQA 550 560 570 580 590 600 VRNEVPEEKL YKVYTFNSVR KSMSTVIRNP NGGFRMYSKG ASEIILRKCN RILDRKGEAV 610 620 630 640 650 660 PFKNKDRDDM VRTVIEPMAC DGLRTICIAY RDFDDTEPSW DNENEILTEL TCIAVVGIED 670 680 690 700 710 720 PVRPEVPDAI AKCKQAGITV RMVTGDNINT ARAIATKCGI LTPGDDFLCL EGKEFNRLIR 730 740 750 760 770 780 NEKGEVEQEK LDKIWPKLRV LARSSPTDKH TLVKGIIDST VGEHRQVVAV TGDGTNDGPA 790 800 810 820 830 840 LKKADVGFAM GIAGTDVAKE ASDIILTDDN FTSIVKAVMW GRNVYDSISK FLQFQLTVNV 850 860 870 880 890 900 VAVIVAFTGA CITQDSPLKA VQMLWVNLIM DTFASLALAT EPPTESLLKR RPYGRNKPLI 910 920 930 940 950 960 SRTMMKNILG HAFYQLIVIF ILVFAGEKFF DIDSGRKAPL HSPPSQHYTI VFNTFVLMQL 970 980 990 1000 1010 1020 FNEINSRKIH GEKNVFSGIY RNIIFCSVVL GTFICQIFIV EFGGKPFSCT SLSLSQWLWC 1030 1040 1050 1060 1070 1080 LFIGIGELLW GQFISAIPTR SLKFLKEAGH GTTKEEITKD AEGLDEIDHA EMELRRGQIL 1090 1100 1110 1120 1130 1140 WFRGLNRIQT QIDVINTFQT GASFKGVLRR QNMGQHLDVK LVPSSSYVAV APVKSSPTTS 1150 VPAVSSPPMG NQSGQSVP
	Isoform XK (XG). Checksum: 088F8CB994D46238	FASTA	1,134	125,344
	10 20 30 40 50 60 MTNPSDRVLP ANSMAESREG DFGCTVMELR KLMELRSRDA LTQINVHYGG VQNLCSRLKT 70 80 90 100 110 120 SPVEGLSGNP ADLEKRRQVF GHNVIPPKKP KTFLELVWEA LQDVTLIILE IAAIISLVLS 130 140 150 160 170 180 FYRPAGEENE LCGQVATTPE DENEAQAGWI EGAAILFSVI IVVLVTAFND WSKEKQFRGL 190 200 210 220 230 240 QCRIEQEQKF SIIRNGQLIQ LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT 250 260 270 280 290 300 GESDHVKKSL DKDPMLLSGT HVMEGSGRMV VTAVGVNSQT GIILTLLGVN EDDEGEKKKK 310 320 330 340 350 360 GKKQGVPENR NKAKTQDGVA LEIQPLNSQE GIDNEEKDKK AVKVPKKEKS VLQGKLTRLA 370 380 390 400 410 420 VQIGKAGLLM SALTVFILIL YFVIDNFVIN RRPWLPECTP IYIQYFVKFF IIGITVLVVA 430 440 450 460 470 480 VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL DACETMGNAT AICSDKTGTL TMNRMTVVQA 490 500 510 520 530 540 YIGGIHYRQI PSPDVFLPKV LDLIVNGISI NSAYTSKILP PEKEGGLPRQ VGNKTECALL 550 560 570 580 590 600 GFVTDLKQDY QAVRNEVPEE KLYKVYTFNS VRKSMSTVIR NPNGGFRMYS KGASEIILRK 610 620 630 640 650 660 CNRILDRKGE AVPFKNKDRD DMVRTVIEPM ACDGLRTICI AYRDFDDTEP SWDNENEILT 670 680 690 700 710 720 ELTCIAVVGI EDPVRPEVPD AIAKCKQAGI TVRMVTGDNI NTARAIATKC GILTPGDDFL 730 740 750 760 770 780 CLEGKEFNRL IRNEKGEVEQ EKLDKIWPKL RVLARSSPTD KHTLVKGIID STVGEHRQVV 790 800 810 820 830 840 AVTGDGTNDG PALKKADVGF AMGIAGTDVA KEASDIILTD DNFTSIVKAV MWGRNVYDSI 850 860 870 880 890 900 SKFLQFQLTV NVVAVIVAFT GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTESLL 910 920 930 940 950 960 KRRPYGRNKP LISRTMMKNI LGHAFYQLIV IFILVFAGEK FFDIDSGRKA PLHSPPSQHY 970 980 990 1000 1010 1020 TIVFNTFVLM QLFNEINSRK IHGEKNVFSG IYRNIIFCSV VLGTFICQFI SAIPTRSLKF 1030 1040 1050 1060 1070 1080 LKEAGHGTTK EEITKDAEGL DEIDHAEMEL RRGQILWFRG LNRIQTQIDV INTFQTGASF 1090 1100 1110 1120 1130 KGVLRRQNMG QHLDVKLVPS SSYVAVAPVK SSPTTSVPAV SSPPMGNQSG QSVP
	Isoform ZK (ZG). Checksum: 4AD4378ED226853B	FASTA	1,122	124,007
	10 20 30 40 50 60 MTNPSDRVLP ANSMAESREG DFGCTVMELR KLMELRSRDA LTQINVHYGG VQNLCSRLKT 70 80 90 100 110 120 SPVEGLSGNP ADLEKRRQVF GHNVIPPKKP KTFLELVWEA LQDVTLIILE IAAIISLVLS 130 140 150 160 170 180 FYRPAGEENE LCGQVATTPE DENEAQAGWI EGAAILFSVI IVVLVTAFND WSKEKQFRGL 190 200 210 220 230 240 QCRIEQEQKF SIIRNGQLIQ LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT 250 260 270 280 290 300 GESDHVKKSL DKDPMLLSGT HVMEGSGRMV VTAVGVNSQT GIILTLLGVN EDDEGEKKKK 310 320 330 340 350 360 AKTQDGVALE IQPLNSQEGI DNEEKDKKAV KVPKKEKSVL QGKLTRLAVQ IGKAGLLMSA 370 380 390 400 410 420 LTVFILILYF VIDNFVINRR PWLPECTPIY IQYFVKFFII GITVLVVAVP EGLPLAVTIS 430 440 450 460 470 480 LAYSVKKMMK DNNLVRHLDA CETMGNATAI CSDKTGTLTM NRMTVVQAYI GGIHYRQIPS 490 500 510 520 530 540 PDVFLPKVLD LIVNGISINS AYTSKILPPE KEGGLPRQVG NKTECALLGF VTDLKQDYQA 550 560 570 580 590 600 VRNEVPEEKL YKVYTFNSVR KSMSTVIRNP NGGFRMYSKG ASEIILRKCN RILDRKGEAV 610 620 630 640 650 660 PFKNKDRDDM VRTVIEPMAC DGLRTICIAY RDFDDTEPSW DNENEILTEL TCIAVVGIED 670 680 690 700 710 720 PVRPEVPDAI AKCKQAGITV RMVTGDNINT ARAIATKCGI LTPGDDFLCL EGKEFNRLIR 730 740 750 760 770 780 NEKGEVEQEK LDKIWPKLRV LARSSPTDKH TLVKGIIDST VGEHRQVVAV TGDGTNDGPA 790 800 810 820 830 840 LKKADVGFAM GIAGTDVAKE ASDIILTDDN FTSIVKAVMW GRNVYDSISK FLQFQLTVNV 850 860 870 880 890 900 VAVIVAFTGA CITQDSPLKA VQMLWVNLIM DTFASLALAT EPPTESLLKR RPYGRNKPLI 910 920 930 940 950 960 SRTMMKNILG HAFYQLIVIF ILVFAGEKFF DIDSGRKAPL HSPPSQHYTI VFNTFVLMQL 970 980 990 1000 1010 1020 FNEINSRKIH GEKNVFSGIY RNIIFCSVVL GTFICQFISA IPTRSLKFLK EAGHGTTKEE 1030 1040 1050 1060 1070 1080 ITKDAEGLDE IDHAEMELRR GQILWFRGLN RIQTQIDVIN TFQTGASFKG VLRRQNMGQH 1090 1100 1110 1120 LDVKLVPSSS YVAVAPVKSS PTTSVPAVSS PPMGNQSGQS VP
	Isoform XB (AIICI). Checksum: 76CE806974035974	FASTA	1,205	133,931
	10 20 30 40 50 60 MTNPSDRVLP ANSMAESREG DFGCTVMELR KLMELRSRDA LTQINVHYGG VQNLCSRLKT 70 80 90 100 110 120 SPVEGLSGNP ADLEKRRQVF GHNVIPPKKP KTFLELVWEA LQDVTLIILE IAAIISLVLS 130 140 150 160 170 180 FYRPAGEENE LCGQVATTPE DENEAQAGWI EGAAILFSVI IVVLVTAFND WSKEKQFRGL 190 200 210 220 230 240 QCRIEQEQKF SIIRNGQLIQ LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT 250 260 270 280 290 300 GESDHVKKSL DKDPMLLSGT HVMEGSGRMV VTAVGVNSQT GIILTLLGVN EDDEGEKKKK 310 320 330 340 350 360 GKKQGVPENR NKAKTQDGVA LEIQPLNSQE GIDNEEKDKK AVKVPKKEKS VLQGKLTRLA 370 380 390 400 410 420 VQIGKAGLLM SALTVFILIL YFVIDNFVIN RRPWLPECTP IYIQYFVKFF IIGITVLVVA 430 440 450 460 470 480 VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL DACETMGNAT AICSDKTGTL TMNRMTVVQA 490 500 510 520 530 540 YIGGIHYRQI PSPDVFLPKV LDLIVNGISI NSAYTSKILP PEKEGGLPRQ VGNKTECALL 550 560 570 580 590 600 GFVTDLKQDY QAVRNEVPEE KLYKVYTFNS VRKSMSTVIR NPNGGFRMYS KGASEIILRK 610 620 630 640 650 660 CNRILDRKGE AVPFKNKDRD DMVRTVIEPM ACDGLRTICI AYRDFDDTEP SWDNENEILT 670 680 690 700 710 720 ELTCIAVVGI EDPVRPEVPD AIAKCKQAGI TVRMVTGDNI NTARAIATKC GILTPGDDFL 730 740 750 760 770 780 CLEGKEFNRL IRNEKGEVEQ EKLDKIWPKL RVLARSSPTD KHTLVKGIID STVGEHRQVV 790 800 810 820 830 840 AVTGDGTNDG PALKKADVGF AMGIAGTDVA KEASDIILTD DNFTSIVKAV MWGRNVYDSI 850 860 870 880 890 900 SKFLQFQLTV NVVAVIVAFT GACITQDSPL KAVQMLWVNL IMDTFASLAL ATEPPTESLL 910 920 930 940 950 960 KRRPYGRNKP LISRTMMKNI LGHAFYQLIV IFILVFAGEK FFDIDSGRKA PLHSPPSQHY 970 980 990 1000 1010 1020 TIVFNTFVLM QLFNEINSRK IHGEKNVFSG IYRNIIFCSV VLGTFICQIF IVEFGGKPFS 1030 1040 1050 1060 1070 1080 CTSLSLSQWL WCLFIGIGEL LWGQFISAIP TRSLKFLKEA GHGTTKEEIT KDAEGLDEID 1090 1100 1110 1120 1130 1140 HAEMELRRGQ ILWFRGLNRI QTQIKVVKAF HSSLHESIQK PYNQKSIHSF MTHPEFAIEE 1150 1160 1170 1180 1190 1200 ELPRTPLLDE EEEENPDKAS KFGTRVLLLD GEVTPYANTN NNAVDCNQVQ LPQSDSSLQS LETSV
	Isoform ZB (AICI). Checksum: 6AA34BD1850CADB7	FASTA	1,193	132,594
	10 20 30 40 50 60 MTNPSDRVLP ANSMAESREG DFGCTVMELR KLMELRSRDA LTQINVHYGG VQNLCSRLKT 70 80 90 100 110 120 SPVEGLSGNP ADLEKRRQVF GHNVIPPKKP KTFLELVWEA LQDVTLIILE IAAIISLVLS 130 140 150 160 170 180 FYRPAGEENE LCGQVATTPE DENEAQAGWI EGAAILFSVI IVVLVTAFND WSKEKQFRGL 190 200 210 220 230 240 QCRIEQEQKF SIIRNGQLIQ LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT 250 260 270 280 290 300 GESDHVKKSL DKDPMLLSGT HVMEGSGRMV VTAVGVNSQT GIILTLLGVN EDDEGEKKKK 310 320 330 340 350 360 AKTQDGVALE IQPLNSQEGI DNEEKDKKAV KVPKKEKSVL QGKLTRLAVQ IGKAGLLMSA 370 380 390 400 410 420 LTVFILILYF VIDNFVINRR PWLPECTPIY IQYFVKFFII GITVLVVAVP EGLPLAVTIS 430 440 450 460 470 480 LAYSVKKMMK DNNLVRHLDA CETMGNATAI CSDKTGTLTM NRMTVVQAYI GGIHYRQIPS 490 500 510 520 530 540 PDVFLPKVLD LIVNGISINS AYTSKILPPE KEGGLPRQVG NKTECALLGF VTDLKQDYQA 550 560 570 580 590 600 VRNEVPEEKL YKVYTFNSVR KSMSTVIRNP NGGFRMYSKG ASEIILRKCN RILDRKGEAV 610 620 630 640 650 660 PFKNKDRDDM VRTVIEPMAC DGLRTICIAY RDFDDTEPSW DNENEILTEL TCIAVVGIED 670 680 690 700 710 720 PVRPEVPDAI AKCKQAGITV RMVTGDNINT ARAIATKCGI LTPGDDFLCL EGKEFNRLIR 730 740 750 760 770 780 NEKGEVEQEK LDKIWPKLRV LARSSPTDKH TLVKGIIDST VGEHRQVVAV TGDGTNDGPA 790 800 810 820 830 840 LKKADVGFAM GIAGTDVAKE ASDIILTDDN FTSIVKAVMW GRNVYDSISK FLQFQLTVNV 850 860 870 880 890 900 VAVIVAFTGA CITQDSPLKA VQMLWVNLIM DTFASLALAT EPPTESLLKR RPYGRNKPLI 910 920 930 940 950 960 SRTMMKNILG HAFYQLIVIF ILVFAGEKFF DIDSGRKAPL HSPPSQHYTI VFNTFVLMQL 970 980 990 1000 1010 1020 FNEINSRKIH GEKNVFSGIY RNIIFCSVVL GTFICQIFIV EFGGKPFSCT SLSLSQWLWC 1030 1040 1050 1060 1070 1080 LFIGIGELLW GQFISAIPTR SLKFLKEAGH GTTKEEITKD AEGLDEIDHA EMELRRGQIL 1090 1100 1110 1120 1130 1140 WFRGLNRIQT QIKVVKAFHS SLHESIQKPY NQKSIHSFMT HPEFAIEEEL PRTPLLDEEE 1150 1160 1170 1180 1190 EENPDKASKF GTRVLLLDGE VTPYANTNNN AVDCNQVQLP QSDSSLQSLE TSV
	Isoform ZD (AICIV). Checksum: 5E9E48B86464E926	FASTA	1,229	136,584
	10 20 30 40 50 60 MTNPSDRVLP ANSMAESREG DFGCTVMELR KLMELRSRDA LTQINVHYGG VQNLCSRLKT 70 80 90 100 110 120 SPVEGLSGNP ADLEKRRQVF GHNVIPPKKP KTFLELVWEA LQDVTLIILE IAAIISLVLS 130 140 150 160 170 180 FYRPAGEENE LCGQVATTPE DENEAQAGWI EGAAILFSVI IVVLVTAFND WSKEKQFRGL 190 200 210 220 230 240 QCRIEQEQKF SIIRNGQLIQ LPVAEIVVGD IAQVKYGDLL PADGILIQGN DLKIDESSLT 250 260 270 280 290 300 GESDHVKKSL DKDPMLLSGT HVMEGSGRMV VTAVGVNSQT GIILTLLGVN EDDEGEKKKK 310 320 330 340 350 360 AKTQDGVALE IQPLNSQEGI DNEEKDKKAV KVPKKEKSVL QGKLTRLAVQ IGKAGLLMSA 370 380 390 400 410 420 LTVFILILYF VIDNFVINRR PWLPECTPIY IQYFVKFFII GITVLVVAVP EGLPLAVTIS 430 440 450 460 470 480 LAYSVKKMMK DNNLVRHLDA CETMGNATAI CSDKTGTLTM NRMTVVQAYI GGIHYRQIPS 490 500 510 520 530 540 PDVFLPKVLD LIVNGISINS AYTSKILPPE KEGGLPRQVG NKTECALLGF VTDLKQDYQA 550 560 570 580 590 600 VRNEVPEEKL YKVYTFNSVR KSMSTVIRNP NGGFRMYSKG ASEIILRKCN RILDRKGEAV 610 620 630 640 650 660 PFKNKDRDDM VRTVIEPMAC DGLRTICIAY RDFDDTEPSW DNENEILTEL TCIAVVGIED 670 680 690 700 710 720 PVRPEVPDAI AKCKQAGITV RMVTGDNINT ARAIATKCGI LTPGDDFLCL EGKEFNRLIR 730 740 750 760 770 780 NEKGEVEQEK LDKIWPKLRV LARSSPTDKH TLVKGIIDST VGEHRQVVAV TGDGTNDGPA 790 800 810 820 830 840 LKKADVGFAM GIAGTDVAKE ASDIILTDDN FTSIVKAVMW GRNVYDSISK FLQFQLTVNV 850 860 870 880 890 900 VAVIVAFTGA CITQDSPLKA VQMLWVNLIM DTFASLALAT EPPTESLLKR RPYGRNKPLI 910 920 930 940 950 960 SRTMMKNILG HAFYQLIVIF ILVFAGEKFF DIDSGRKAPL HSPPSQHYTI VFNTFVLMQL 970 980 990 1000 1010 1020 FNEINSRKIH GEKNVFSGIY RNIIFCSVVL GTFICQIFIV EFGGKPFSCT SLSLSQWLWC 1030 1040 1050 1060 1070 1080 LFIGIGELLW GQFISAIPTR SLKFLKEAGH GTTKEEITKD AEGLDEIDHA EMELRRGQIL 1090 1100 1110 1120 1130 1140 WFRGLNRIQT QIDVINTFQT GASFKGVLRR QNMGQHLDVK LVPSSSYIKV VKAFHSSLHE 1150 1160 1170 1180 1190 1200 SIQKPYNQKS IHSFMTHPEF AIEEELPRTP LLDEEEEENP DKASKFGTRV LLLDGEVTPY 1210 1220 ANTNNNAVDC NQVQLPQSDS SLQSLETSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Peptide sequence analysis and molecular cloning reveal two calcium pump isoforms in the human erythrocyte membrane." Strehler E.E., James P., Fischer R., Heim R., Vorherr T.E., Filoteo A.G., Penniston J.T., Carafoli E. J. Biol. Chem. 265:2835-2842(1990) [PubMed: 2137451] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM XB), PARTIAL PROTEIN SEQUENCE. Tissue: Erythrocyte.
[2]	"Analysis of the tissue-specific distribution of mRNAs encoding the plasma membrane calcium-pumping ATPases and characterization of an alternately spliced form of PMCA4 at the cDNA and genomic levels." Brandt P., Neve R.L., Kammesheidt A., Rhoads R.E., Vanaman T.C. J. Biol. Chem. 267:4376-4385(1992) [PubMed: 1531651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM XA). Tissue: Fetal brain.
[3]	"Analysis of mRNA expression and cloning of a novel plasma membrane Ca(2+)-ATPase splice variant in human heart." Santiago-Garcia J., Mas-Oliva J., Saavedra D., Zarain-Herzberg A. Mol. Cell. Biochem. 155:173-182(1996) [PubMed: 8700162] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS XA; XB; XK; ZA; ZB AND ZK). Tissue: Heart muscle.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Identification and primary structure of a calmodulin binding domain of the Ca2+ pump of human erythrocytes." James P., Maeda M., Fischer R., Verma A.K., Krebs J., Penniston J.T., Carafoli E. J. Biol. Chem. 263:2905-2910(1988) [PubMed: 2963820] [Abstract] Cited for: PROTEIN SEQUENCE OF 1085-1153 (ISOFORMS XB/ZB), CALMODULIN-BINDING SUBDOMAIN A.
[7]	"A C-terminal, calmodulin-like regulatory domain from the plasma membrane Ca2+-pumping ATPase." Brandt P., Zurini M., Neve R.L., Rhoads R.E., Vanaman T.C. Proc. Natl. Acad. Sci. U.S.A. 85:2914-2918(1988) [PubMed: 2966397] [Abstract] Cited for: PROTEIN SEQUENCE OF 1177-1190.
[8]	"Quantitative analysis of alternative splicing options of human plasma membrane calcium pump genes." Stauffer T.P., Hilfiker H., Carafoli E., Strehler E.E. J. Biol. Chem. 268:25993-26003(1993) [PubMed: 8245032] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS X AND Z). Tissue: Heart.
[9]	Erratum Stauffer T.P., Hilfiker H., Carafoli E., Strehler E.E. J. Biol. Chem. 269:32022-32022(1994) [PubMed: 7989379] [Abstract]
[10]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[11]	"NMR solution structure of a complex of calmodulin with a binding peptide of the Ca(2+) pump." Elshorst B., Hennig M., Foersterling H., Diener A., Maurer M., Schulte P., Schwalbe H., Griesinger C., Krebs J., Schmid H., Vorherr T.E., Carafoli E. Biochemistry 38:12320-12332(1999) [PubMed: 10493800] [Abstract] Cited for: STRUCTURE BY NMR OF 1086-1104 IN COMPLEX WITH CALMODULIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M25874 mRNA. Translation: AAA50819.1.
M83363 mRNA. Translation: AAA36455.1.
U42026 mRNA. Translation: AAB17577.1.
U42061 mRNA. Translation: AAB17578.1.
U42062 mRNA. Translation: AAB17579.1.
U42378 mRNA. Translation: AAB17580.1.
AL513343, AC114402 Genomic DNA. Translation: CAI17026.1.
CH471067 Genomic DNA. Translation: EAW91486.1.

IPI

IPI00012490.
IPI00217164.
IPI00217165.
IPI00217166.
IPI00217168.
IPI00217169.
IPI00217170.
IPI00217171.

PIR

A35547.

RefSeq

NP_001001396.1.
NP_001675.3.

UniGene

Hs.343522

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CFF	NMR	-	B	1086-1104	[»]
2KNE	NMR	-	B	1086-1104	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6128N.

IntAct

P23634. 4 interactions.

STRING

P23634.

Protein family/group databases

TCDB

3.A.3.2.1. P-type ATPase (P-ATPase) superfamily.

PTM databases

PhosphoSite

P23634.

Proteomic databases

PRIDE

P23634.

Genome annotation databases

Ensembl

ENST00000341360; ENSP00000340930; ENSG00000058668; Homo sapiens. [Genome view]
ENST00000367218; ENSP00000356187; ENSG00000058668; Homo sapiens. [Genome view]

GeneID

493.

KEGG

hsa:493.

UCSC

uc001gzv.1. human.
uc001gzw.1. human.

Organism-specific databases

CTD

493.

GeneCards

GC01P201862.

H-InvDB

HIX0023651.

HGNC

HGNC:817. ATP2B4.

HPA

CAB016118.

MIM

108732. gene.

PharmGKB

PA25110.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P23634.

InParanoid

P23634.

OMA

LKTNPVE.

OrthoDB

EOG9KM1NM.

PhylomeDB

P23634.

Enzyme and pathway databases

BRENDA

3.6.3.8. 247.

Gene expression databases

ArrayExpress

P23634.

Bgee

P23634.

CleanEx

HS_ATP2B2.
HS_ATP2B4.

Genevestigator

P23634.

GermOnline

ENSG00000058668. Homo sapiens.

Family and domain databases

InterPro

IPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR006408. ATPase_P-typ_Ca-transp_PMCA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR005834. Dehalogen-like_hydro.
[Graphical view]

PANTHER

PTHR11939. ATPase_P. 1 hit.

Pfam

PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]

PRINTS

PR00119. CATATPASE.

SMART

SM00831. Cation_ATPase_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 4 hits.

PROSITE

PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

SOURCE

Search...

Entry information

Entry name

AT2B4_HUMAN

Accession

Primary (citable) accession number: P23634
Secondary accession number(s): B1APW6 , Q13450, Q13452, Q13455, Q16817

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.