Reviewed, UniProtKB/Swiss-Prot P23623 (OXLA_NEUCR)

Names and origin

Protein names

Recommended name:
    L-amino-acid oxidase
      Short name=LAAO
      Short name=LAO
    EC=1.4.3.2

Gene names

Name:	lox
ORF Names:	B14A6.230, NCU01066

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	696 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	An L-amino acid + H(2)O + O(2) = a 2-oxo acid + NH(3) + H(2)O(2).
Cofactor	FAD.
Induction	By addition of L-amino acids after nitrogen starvation, by starvation in phosphate buffer and by the addition of protein synthesis inhibitors, D-amino acids, or ATP.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Zymogen
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-amino-acid oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 130	130			PRO_0000001708
	Chain	131 – 696	566	L-amino-acid oxidase		PRO_0000001709
Regions
	Nucleotide binding	236 – 237	2	FAD By similarity
	Nucleotide binding	658 – 661	4	FAD By similarity
Sites
	Binding site	207	1	FAD By similarity
	Binding site	215	1	FAD By similarity
	Binding site	237	1	Substrate By similarity
	Binding site	440	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	564	1	Substrate By similarity
	Binding site	649	1	FAD By similarity
Experimental info
	Sequence conflict	6 – 13	8	AAGAALLA → RGCGTAR AA sequence Ref.1
	Sequence conflict	53	1	I → L AA sequence Ref.1
	Sequence conflict	343	1	F → L AA sequence Ref.1
	Sequence conflict	391 – 414	24	SASAH…STWKT → APALTRTGTRCTKGCTFPRR RGA AA sequence Ref.1
	Sequence conflict	440	1	V → L AA sequence Ref.1
	Sequence conflict	684 – 685	2	AT → GRP AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P23623-1 [UniParc]. Last modified April 30, 2003. Version 2. Checksum: 3909C59198E96008	FASTA	696	77,013
	10 20 30 40 50 60 MKWSAAAGAA LLALPANSAV TASLPLKLET RSSLNSRLSN IHVERSASVE GAISYTYGSC 70 80 90 100 110 120 QAKREEEAHH SISQPTDAHH DRLVWVIPEN VQSGGCISAW SRANGRLVGR SRPQSFDFKS 130 140 150 160 170 180 IKMRRDLKAR ATKPSDSVAI HMTTDNGINP WGPWFDGVKL LEDKEISTVD VEKAKSKNIA 190 200 210 220 230 240 IVGAGMSGLM TYLCLTQAGM TNVSIIEGGN RLGGRVHTEY LSGGPFDYSY QEMGPMRFPN 250 260 270 280 290 300 TITLGNETYN VSDHQLVFQL AEEMNSLNGH SKNLSVDFIP WYQSNSNGLY YYDGIKNPET 310 320 330 340 350 360 GLPPTLAELA ANSSLALTRV SNNSTKSLSQ KVDAFLPDTD KFFAEMAQNM FKAHADWLSG 370 380 390 400 410 420 GLAGLPGDQW SEFGFMVNYL RGSLNDTAFL SASAHSYWDT LYEGMYFSAS TWKTIDGGLN 430 440 450 460 470 480 RLPLSFHPLV DNATTLNRRV ERVAFDAETQ KVTLHSRNSY KDSFESSEHD YAVIAAPFSI 490 500 510 520 530 540 VKKWRFSPAL DLTAPTLANA IQNLEYTSAC KVALEFRTRF WEHLPQPIYG SCSTTSDIPG 550 560 570 580 590 600 IGSICYPSYN INGTDGPASI LASYISGADW GDRWVSTPEE EHVQYVLNAM AEIHGEELVK 610 620 630 640 650 660 EQYTGQFNRR CWALDPLESA SWASPTVGQH ELYLPEYFQT RNNLVFVGEH TSYTHAWIAS 670 680 690 ALESGIRGSV QLLLELGLVD EAKATVDKWM ARWIDV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the L-amino-acid oxidase gene from Neurospora crassa." Niedermann D.M., Lerch K. J. Biol. Chem. 265:17246-17251(1990) [PubMed: 2145270] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL670007 Genomic DNA. Translation: CAD21325.1. AABX02000015 Genomic DNA. Translation: EAA32442.1.
PIR	A38314.
RefSeq	XP_961678.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3877869.
KEGG	ncr:NCU01066.
NMPDR	fig|5141.1.peg.4492.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-004776-MON.
Family and domain databases
InterPro	IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OXLA_NEUCR

Accession

Primary (citable) accession number: P23623
Secondary accession number(s): Q7RVE5, Q8X0D4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	April 30, 2003
Last modified:	November 25, 2008
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)