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P23623

- OXLA_NEUCR

UniProt

P23623 - OXLA_NEUCR

Protein

L-amino-acid oxidase

Gene

lox

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 2 (30 Apr 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

    Cofactori

    FAD.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei207 – 2071FADBy similarity
    Binding sitei215 – 2151FADBy similarity
    Binding sitei237 – 2371SubstrateBy similarity
    Binding sitei440 – 4401FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei564 – 5641SubstrateBy similarity
    Binding sitei649 – 6491FADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi236 – 2372FADBy similarity
    Nucleotide bindingi658 – 6614FADBy similarity

    GO - Molecular functioni

    1. L-amino-acid oxidase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-amino-acid oxidase (EC:1.4.3.2)
    Short name:
    LAAO
    Short name:
    LAO
    Gene namesi
    Name:lox
    ORF Names:B14A6.230, NCU01066
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 2, Linkage Group V

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 130130PRO_0000001708Add
    BLAST
    Chaini131 – 696566L-amino-acid oxidasePRO_0000001709Add
    BLAST

    Keywords - PTMi

    Zymogen

    Expressioni

    Inductioni

    By addition of L-amino acids after nitrogen starvation, by starvation in phosphate buffer and by the addition of protein synthesis inhibitors, D-amino acids, or ATP.

    Interactioni

    Protein-protein interaction databases

    STRINGi5141.NCU01066.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP23623.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the flavin monoamine oxidase family.Curated

    Phylogenomic databases

    eggNOGiCOG1231.
    HOGENOMiHOG000178764.
    OMAiHCQFDEG.
    OrthoDBiEOG7FNCH3.

    Family and domain databases

    InterProiIPR002937. Amino_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23623-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKWSAAAGAA LLALPANSAV TASLPLKLET RSSLNSRLSN IHVERSASVE    50
    GAISYTYGSC QAKREEEAHH SISQPTDAHH DRLVWVIPEN VQSGGCISAW 100
    SRANGRLVGR SRPQSFDFKS IKMRRDLKAR ATKPSDSVAI HMTTDNGINP 150
    WGPWFDGVKL LEDKEISTVD VEKAKSKNIA IVGAGMSGLM TYLCLTQAGM 200
    TNVSIIEGGN RLGGRVHTEY LSGGPFDYSY QEMGPMRFPN TITLGNETYN 250
    VSDHQLVFQL AEEMNSLNGH SKNLSVDFIP WYQSNSNGLY YYDGIKNPET 300
    GLPPTLAELA ANSSLALTRV SNNSTKSLSQ KVDAFLPDTD KFFAEMAQNM 350
    FKAHADWLSG GLAGLPGDQW SEFGFMVNYL RGSLNDTAFL SASAHSYWDT 400
    LYEGMYFSAS TWKTIDGGLN RLPLSFHPLV DNATTLNRRV ERVAFDAETQ 450
    KVTLHSRNSY KDSFESSEHD YAVIAAPFSI VKKWRFSPAL DLTAPTLANA 500
    IQNLEYTSAC KVALEFRTRF WEHLPQPIYG SCSTTSDIPG IGSICYPSYN 550
    INGTDGPASI LASYISGADW GDRWVSTPEE EHVQYVLNAM AEIHGEELVK 600
    EQYTGQFNRR CWALDPLESA SWASPTVGQH ELYLPEYFQT RNNLVFVGEH 650
    TSYTHAWIAS ALESGIRGSV QLLLELGLVD EAKATVDKWM ARWIDV 696
    Length:696
    Mass (Da):77,013
    Last modified:April 30, 2003 - v2
    Checksum:i3909C59198E96008
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 138AAGAALLA → RGCGTAR AA sequence (PubMed:2145270)Curated
    Sequence conflicti53 – 531I → L AA sequence (PubMed:2145270)Curated
    Sequence conflicti343 – 3431F → L AA sequence (PubMed:2145270)Curated
    Sequence conflicti391 – 41424SASAH…STWKT → APALTRTGTRCTKGCTFPRR RGA AA sequence (PubMed:2145270)CuratedAdd
    BLAST
    Sequence conflicti440 – 4401V → L AA sequence (PubMed:2145270)Curated
    Sequence conflicti684 – 6852AT → GRP AA sequence (PubMed:2145270)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL670007 Genomic DNA. Translation: CAD21325.1.
    CM002240 Genomic DNA. Translation: EAA32442.1.
    PIRiA38314.
    RefSeqiXP_961678.1. XM_956585.1.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000004415; EFNCRP00000004415; EFNCRG00000004409.
    GeneIDi3877869.
    KEGGincr:NCU01066.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL670007 Genomic DNA. Translation: CAD21325.1 .
    CM002240 Genomic DNA. Translation: EAA32442.1 .
    PIRi A38314.
    RefSeqi XP_961678.1. XM_956585.1.

    3D structure databases

    ProteinModelPortali P23623.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU01066.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000004415 ; EFNCRP00000004415 ; EFNCRG00000004409 .
    GeneIDi 3877869.
    KEGGi ncr:NCU01066.

    Phylogenomic databases

    eggNOGi COG1231.
    HOGENOMi HOG000178764.
    OMAi HCQFDEG.
    OrthoDBi EOG7FNCH3.

    Family and domain databases

    InterProi IPR002937. Amino_oxidase.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the L-amino-acid oxidase gene from Neurospora crassa."
      Niedermann D.M., Lerch K.
      J. Biol. Chem. 265:17246-17251(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    3. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiOXLA_NEUCR
    AccessioniPrimary (citable) accession number: P23623
    Secondary accession number(s): Q7RVE5, Q8X0D4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 93 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3