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Protein

Transcription elongation factor SPT6

Gene

SPT6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression. Essential for viability.4 Publications

GO - Molecular functioni

  • DNA binding Source: InterPro
  • histone binding Source: SGD
  • nucleosome binding Source: SGD
  • transcription antitermination factor activity, DNA binding Source: SGD
  • transcription factor activity, core RNA polymerase II binding Source: SGD

GO - Biological processi

  • chromatin maintenance Source: SGD
  • chromatin organization Source: SGD
  • chromatin remodeling Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter by glucose Source: SGD
  • nucleosome assembly Source: SGD
  • nucleosome organization Source: SGD
  • poly(A)+ mRNA export from nucleus Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
  • regulation of histone H3-K36 methylation Source: SGD
  • regulation of mRNA 3'-end processing Source: SGD
  • regulation of nucleosome density Source: SGD
  • regulation of transcriptional start site selection at RNA polymerase II promoter Source: SGD
  • regulation of transcription from RNA polymerase II promoter in response to stress Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • transcription antitermination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30823-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor SPT6
Alternative name(s):
Chromatin elongation factor SPT6
Gene namesi
Name:SPT6
Synonyms:CRE2, SSN20
Ordered Locus Names:YGR116W
ORF Names:G6169
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR116W.
SGDiS000003348. SPT6.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • transcriptionally active chromatin Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14511451Transcription elongation factor SPT6PRO_0000072173Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei94 – 941PhosphoserineCombined sources
Modified residuei134 – 1341PhosphoserineCombined sources
Modified residuei136 – 1361PhosphoserineCombined sources
Modified residuei148 – 1481PhosphoserineCombined sources
Modified residuei155 – 1551PhosphoserineCombined sources
Modified residuei206 – 2061PhosphoserineCombined sources
Modified residuei295 – 2951PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23615.

PTM databases

iPTMnetiP23615.

Interactioni

Subunit structurei

Interacts with CTR9.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SPN1Q065057EBI-17947,EBI-32596
SPT5P276922EBI-17947,EBI-17937

GO - Molecular functioni

  • histone binding Source: SGD
  • nucleosome binding Source: SGD

Protein-protein interaction databases

BioGridi33361. 70 interactions.
DIPiDIP-2630N.
IntActiP23615. 21 interactions.
MINTiMINT-427185.

Structurei

Secondary structure

1
1451
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi239 – 24911Combined sources
Helixi256 – 2627Combined sources
Helixi301 – 3088Combined sources
Helixi312 – 3198Combined sources
Beta strandi320 – 3223Combined sources
Helixi324 – 3296Combined sources
Helixi330 – 3323Combined sources
Turni334 – 3374Combined sources
Helixi341 – 35919Combined sources
Helixi368 – 38215Combined sources
Helixi388 – 3947Combined sources
Helixi396 – 3983Combined sources
Beta strandi402 – 4065Combined sources
Helixi411 – 44131Combined sources
Helixi446 – 4549Combined sources
Helixi465 – 47612Combined sources
Helixi478 – 4836Combined sources
Helixi502 – 5054Combined sources
Helixi511 – 5155Combined sources
Helixi521 – 53010Combined sources
Beta strandi541 – 5433Combined sources
Helixi545 – 55410Combined sources
Turni555 – 5595Combined sources
Helixi568 – 58316Combined sources
Helixi586 – 59813Combined sources
Beta strandi599 – 6068Combined sources
Helixi608 – 6136Combined sources
Turni615 – 6173Combined sources
Turni619 – 6257Combined sources
Helixi633 – 6364Combined sources
Helixi639 – 64911Combined sources
Beta strandi652 – 6598Combined sources
Helixi662 – 67312Combined sources
Helixi681 – 73353Combined sources
Beta strandi740 – 7445Combined sources
Beta strandi750 – 7545Combined sources
Turni760 – 7623Combined sources
Beta strandi765 – 7706Combined sources
Beta strandi776 – 7827Combined sources
Helixi793 – 80614Combined sources
Beta strandi809 – 8135Combined sources
Helixi819 – 83315Combined sources
Beta strandi839 – 8413Combined sources
Beta strandi846 – 8483Combined sources
Helixi854 – 8585Combined sources
Helixi861 – 8666Combined sources
Helixi872 – 88514Combined sources
Helixi887 – 8926Combined sources
Helixi896 – 9005Combined sources
Helixi908 – 9103Combined sources
Helixi913 – 93119Combined sources
Helixi935 – 9395Combined sources
Helixi942 – 9454Combined sources
Helixi946 – 9505Combined sources
Helixi956 – 96813Combined sources
Helixi978 – 9814Combined sources
Helixi987 – 9937Combined sources
Turni994 – 9963Combined sources
Helixi1015 – 10184Combined sources
Helixi1023 – 10253Combined sources
Helixi1026 – 103611Combined sources
Helixi1041 – 105010Combined sources
Helixi1055 – 10628Combined sources
Helixi1066 – 10705Combined sources
Helixi1075 – 108612Combined sources
Helixi1091 – 110212Combined sources
Beta strandi1103 – 11053Combined sources
Helixi1117 – 11259Combined sources
Turni1129 – 11313Combined sources
Beta strandi1137 – 11459Combined sources
Beta strandi1150 – 11534Combined sources
Beta strandi1159 – 11624Combined sources
Beta strandi1166 – 11694Combined sources
Turni1177 – 11793Combined sources
Beta strandi1185 – 119410Combined sources
Helixi1195 – 11973Combined sources
Beta strandi1199 – 12035Combined sources
Helixi1206 – 12094Combined sources
Turni1222 – 12243Combined sources
Helixi1227 – 124620Combined sources
Turni1247 – 12493Combined sources
Beta strandi1253 – 12553Combined sources
Helixi1264 – 12718Combined sources
Beta strandi1279 – 12835Combined sources
Turni1285 – 12873Combined sources
Beta strandi1288 – 129811Combined sources
Beta strandi1301 – 131111Combined sources
Beta strandi1313 – 13175Combined sources
Beta strandi1320 – 13245Combined sources
Beta strandi1327 – 13315Combined sources
Helixi1332 – 13387Combined sources
Helixi1340 – 135112Combined sources
Helixi1361 – 137212Combined sources
Beta strandi1380 – 13856Combined sources
Beta strandi1387 – 13893Combined sources
Beta strandi1392 – 14009Combined sources
Beta strandi1406 – 14127Combined sources
Beta strandi1414 – 14196Combined sources
Beta strandi1422 – 14265Combined sources
Helixi1427 – 143711Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L3TNMR-A1250-1440[»]
3OAKX-ray2.15C/D239-263[»]
3PSFX-ray2.59A235-1259[»]
3PSIX-ray3.30A239-1451[»]
3PSJX-ray2.70A1247-1451[»]
3PSKX-ray2.10A/B/C/D1247-1451[»]
ProteinModelPortaliP23615.
SMRiP23615. Positions 298-1210, 1219-1440.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23615.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1257 – 135498SH2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi8 – 125Nuclear localization signalSequence analysis
Motifi77 – 859Nuclear localization signalSequence analysis
Motifi120 – 1256Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 484484Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the SPT6 family.Curated
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

GeneTreeiENSGT00510000047446.
HOGENOMiHOG000193710.
InParanoidiP23615.
KOiK11292.
OMAiWNDEREL.
OrthoDBiEOG092C04AI.

Family and domain databases

Gene3Di1.10.150.310. 1 hit.
1.10.3500.10. 2 hits.
3.30.420.140. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR010994. RuvA_2-like.
IPR000980. SH2.
IPR028083. Spt6_acidic_N_dom.
IPR027999. Spt6_dom.
IPR032706. Spt6_HHH.
IPR028088. Spt6_HTH_DNA-bd_dom.
IPR028231. Spt6_YqgF.
IPR023323. Tex-like_dom.
IPR023097. Tex_RuvX-like_dom.
IPR017072. TF_Spt6.
IPR006641. YqgF/RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10145:SF6. PTHR10145:SF6. 2 hits.
PfamiPF14878. DLD. 1 hit.
PF14635. HHH_7. 1 hit.
PF14641. HTH_44. 1 hit.
PF14633. SH2_2. 1 hit.
PF14632. SPT6_acidic. 1 hit.
PF14639. YqgF. 1 hit.
[Graphical view]
PIRSFiPIRSF036947. Spt6. 1 hit.
SMARTiSM00252. SH2. 1 hit.
SM00732. YqgFc. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23615-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEETGDSKLV PRDEEEIVND NDETKAPSEE EEGEDVFDSS EEDEDIDEDE
60 70 80 90 100
DEARKVQEGF IVNDDDENED PGTSISKKRR KHKRREREED DRLSEDDLDL
110 120 130 140 150
LMENAGVERT KASSSSGKFK RLKRVGDEGN AAESESDNVA ASRQDSTSKL
160 170 180 190 200
EDFFSEDEEE EESGLRNGRN NEYGRDEEDH ENRNRTADKG GILDELDDFI
210 220 230 240 250
EDDEFSDEDD ETRQRRIQEK KLLREQSIKQ PTQITGLSSD KIDEMYDIFG
260 270 280 290 300
DGHDYDWALE IENEELENGN DNNEAEEEEI DEETGAIKST KKKISLQDIY
310 320 330 340 350
DLEDLKKNLM TEGDMKIRKT DIPERYQELR AGITDYGNMS SEDQELERNW
360 370 380 390 400
IAEKISVDKN FDANYDLTEF KEAIGNAIKF ITKENLEVPF IYAYRRNYIS
410 420 430 440 450
SREKDGFLLT EDDLWDIVSL DIEFHSLVNK KDYVQRFYAE LHIDDPIVTE
460 470 480 490 500
YFKNQNTASI AELNSLQDIY DYLEFKYANE INEMFINHTG KTGKKHLKNS
510 520 530 540 550
SYEKFKASPL YQAVSDIGIS AEDVGENISS QHQIHPPVDH PSSKPVEVIE
560 570 580 590 600
SILNANSGDL QVFTSNTKLA IDTVQKYYSL ELSKNTKIRE KVRSDFSKYY
610 620 630 640 650
LADVVLTAKG KKEIQKGSLY EDIKYAINRT PMHFRRDPDV FLKMVEAESL
660 670 680 690 700
NLLSVKLHMS SQAQYIEHLF QIALETTNTS DIAIEWNNFR KLAFNQAMDK
710 720 730 740 750
IFQDISQEVK DNLTKNCQKL VAKTVRHKFM TKLDQAPFIP NVRDPKIPKI
760 770 780 790 800
LSLTCGQGRF GADAIIAVYV NRKGDFIRDY KIVDNPFDKT NPEKFEDTLD
810 820 830 840 850
NIIQSCQPNA IGINGPNPKT QKFYKRLQEV LHKKQIVDSR GHTIPIIYVE
860 870 880 890 900
DEVAIRYQNS ERAAQEFPNK PPLVKYCIAL ARYMHSPLLE YANLTSEEVR
910 920 930 940 950
SLSIHPHQNL LSSEQLSWAL ETAFVDIVNL VSVEVNKATD NNYYASALKY
960 970 980 990 1000
ISGFGKRKAI DFLQSLQRLN EPLLARQQLI THNILHKTIF MNSAGFLYIS
1010 1020 1030 1040 1050
WNEKRQKYED LEHDQLDSTR IHPEDYHLAT KVAADALEYD PDTIAEKEEQ
1060 1070 1080 1090 1100
GTMSEFIELL REDPDRRAKL ESLNLESYAE ELEKNTGLRK LNNLNTIVLE
1110 1120 1130 1140 1150
LLDGFEELRN DFHPLQGDEI FQSLTGESEK TFFKGSIIPV RVERFWHNDI
1160 1170 1180 1190 1200
ICTTNSEVEC VVNAQRHAGA QLRRPANEIY EIGKTYPAKV IYIDYANITA
1210 1220 1230 1240 1250
EVSLLDHDVK QQYVPISYSK DPSIWDLKQE LEDAEEERKL MMAEARAKRT
1260 1270 1280 1290 1300
HRVINHPYYF PFNGRQAEDY LRSKERGEFV IRQSSRGDDH LVITWKLDKD
1310 1320 1330 1340 1350
LFQHIDIQEL EKENPLALGK VLIVDNQKYN DLDQIIVEYL QNKVRLLNEM
1360 1370 1380 1390 1400
TSSEKFKSGT KKDVVKFIED YSRVNPNKSV YYFSLNHDNP GWFYLMFKIN
1410 1420 1430 1440 1450
ANSKLYTWNV KLTNTGYFLV NYNYPSVIQL CNGFKTLLKS NSSKNRMNNY

R
Length:1,451
Mass (Da):168,291
Last modified:November 1, 1991 - v1
Checksum:i0BE9922A59BD0483
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34391 Genomic DNA. Translation: AAA35086.1.
Z72899 Genomic DNA. Translation: CAA97124.1.
Z72902 Genomic DNA. Translation: CAA97127.1.
BK006941 Genomic DNA. Translation: DAA08208.1.
PIRiA36468.
RefSeqiNP_011631.1. NM_001181245.1.

Genome annotation databases

EnsemblFungiiYGR116W; YGR116W; YGR116W.
GeneIDi853011.
KEGGisce:YGR116W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34391 Genomic DNA. Translation: AAA35086.1.
Z72899 Genomic DNA. Translation: CAA97124.1.
Z72902 Genomic DNA. Translation: CAA97127.1.
BK006941 Genomic DNA. Translation: DAA08208.1.
PIRiA36468.
RefSeqiNP_011631.1. NM_001181245.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L3TNMR-A1250-1440[»]
3OAKX-ray2.15C/D239-263[»]
3PSFX-ray2.59A235-1259[»]
3PSIX-ray3.30A239-1451[»]
3PSJX-ray2.70A1247-1451[»]
3PSKX-ray2.10A/B/C/D1247-1451[»]
ProteinModelPortaliP23615.
SMRiP23615. Positions 298-1210, 1219-1440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33361. 70 interactions.
DIPiDIP-2630N.
IntActiP23615. 21 interactions.
MINTiMINT-427185.

PTM databases

iPTMnetiP23615.

Proteomic databases

MaxQBiP23615.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR116W; YGR116W; YGR116W.
GeneIDi853011.
KEGGisce:YGR116W.

Organism-specific databases

EuPathDBiFungiDB:YGR116W.
SGDiS000003348. SPT6.

Phylogenomic databases

GeneTreeiENSGT00510000047446.
HOGENOMiHOG000193710.
InParanoidiP23615.
KOiK11292.
OMAiWNDEREL.
OrthoDBiEOG092C04AI.

Enzyme and pathway databases

BioCyciYEAST:G3O-30823-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP23615.
PROiP23615.

Family and domain databases

Gene3Di1.10.150.310. 1 hit.
1.10.3500.10. 2 hits.
3.30.420.140. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR010994. RuvA_2-like.
IPR000980. SH2.
IPR028083. Spt6_acidic_N_dom.
IPR027999. Spt6_dom.
IPR032706. Spt6_HHH.
IPR028088. Spt6_HTH_DNA-bd_dom.
IPR028231. Spt6_YqgF.
IPR023323. Tex-like_dom.
IPR023097. Tex_RuvX-like_dom.
IPR017072. TF_Spt6.
IPR006641. YqgF/RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10145:SF6. PTHR10145:SF6. 2 hits.
PfamiPF14878. DLD. 1 hit.
PF14635. HHH_7. 1 hit.
PF14641. HTH_44. 1 hit.
PF14633. SH2_2. 1 hit.
PF14632. SPT6_acidic. 1 hit.
PF14639. YqgF. 1 hit.
[Graphical view]
PIRSFiPIRSF036947. Spt6. 1 hit.
SMARTiSM00252. SH2. 1 hit.
SM00732. YqgFc. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPT6_YEAST
AccessioniPrimary (citable) accession number: P23615
Secondary accession number(s): D6VUP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: September 7, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8890 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.