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Protein

Transcription elongation factor SPT6

Gene

SPT6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression; the nucleosome reassembly is essential for transcriptional repression. Essential for viability.4 Publications

GO - Molecular functioni

  • DNA binding Source: InterPro
  • histone binding Source: SGD
  • nucleosome binding Source: SGD
  • transcription antitermination factor activity, DNA binding Source: SGD
  • transcription factor activity, core RNA polymerase II binding Source: SGD

GO - Biological processi

  • chromatin maintenance Source: SGD
  • chromatin organization Source: SGD
  • chromatin remodeling Source: SGD
  • chromatin silencing Source: GO_Central
  • mRNA transcription from RNA polymerase II promoter Source: GO_Central
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter by glucose Source: SGD
  • nucleosome assembly Source: SGD
  • nucleosome organization Source: SGD
  • poly(A)+ mRNA export from nucleus Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
  • regulation of histone H3-K36 methylation Source: SGD
  • regulation of mRNA 3'-end processing Source: SGD
  • regulation of nucleosome density Source: SGD
  • regulation of transcriptional start site selection at RNA polymerase II promoter Source: SGD
  • regulation of transcription from RNA polymerase II promoter in response to stress Source: SGD
  • transcription antitermination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30823-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor SPT6
Alternative name(s):
Chromatin elongation factor SPT6
Gene namesi
Name:SPT6
Synonyms:CRE2, SSN20
Ordered Locus Names:YGR116W
ORF Names:G6169
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR116W.
SGDiS000003348. SPT6.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • transcriptionally active chromatin Source: SGD
  • transcription elongation factor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000721731 – 1451Transcription elongation factor SPT6Add BLAST1451

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei94PhosphoserineCombined sources1
Modified residuei134PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Modified residuei148PhosphoserineCombined sources1
Modified residuei155PhosphoserineCombined sources1
Modified residuei206PhosphoserineCombined sources1
Modified residuei295PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23615.
PRIDEiP23615.

PTM databases

iPTMnetiP23615.

Interactioni

Subunit structurei

Interacts with CTR9.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SPN1Q065057EBI-17947,EBI-32596
SPT5P276922EBI-17947,EBI-17937

GO - Molecular functioni

  • histone binding Source: SGD

Protein-protein interaction databases

BioGridi33361. 70 interactors.
DIPiDIP-2630N.
IntActiP23615. 21 interactors.
MINTiMINT-427185.

Structurei

Secondary structure

11451
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi239 – 249Combined sources11
Helixi256 – 262Combined sources7
Helixi301 – 308Combined sources8
Helixi312 – 319Combined sources8
Beta strandi320 – 322Combined sources3
Helixi324 – 329Combined sources6
Helixi330 – 332Combined sources3
Turni334 – 337Combined sources4
Helixi341 – 359Combined sources19
Helixi368 – 382Combined sources15
Helixi388 – 394Combined sources7
Helixi396 – 398Combined sources3
Beta strandi402 – 406Combined sources5
Helixi411 – 441Combined sources31
Helixi446 – 454Combined sources9
Helixi465 – 476Combined sources12
Helixi478 – 483Combined sources6
Helixi502 – 505Combined sources4
Helixi511 – 515Combined sources5
Helixi521 – 530Combined sources10
Beta strandi541 – 543Combined sources3
Helixi545 – 554Combined sources10
Turni555 – 559Combined sources5
Helixi568 – 583Combined sources16
Helixi586 – 598Combined sources13
Beta strandi599 – 606Combined sources8
Helixi608 – 613Combined sources6
Turni615 – 617Combined sources3
Turni619 – 625Combined sources7
Helixi633 – 636Combined sources4
Helixi639 – 649Combined sources11
Beta strandi652 – 659Combined sources8
Helixi662 – 673Combined sources12
Helixi681 – 733Combined sources53
Beta strandi740 – 744Combined sources5
Beta strandi750 – 754Combined sources5
Turni760 – 762Combined sources3
Beta strandi765 – 770Combined sources6
Beta strandi776 – 782Combined sources7
Helixi793 – 806Combined sources14
Beta strandi809 – 813Combined sources5
Helixi819 – 833Combined sources15
Beta strandi839 – 841Combined sources3
Beta strandi846 – 848Combined sources3
Helixi854 – 858Combined sources5
Helixi861 – 866Combined sources6
Helixi872 – 885Combined sources14
Helixi887 – 892Combined sources6
Helixi896 – 900Combined sources5
Helixi908 – 910Combined sources3
Helixi913 – 931Combined sources19
Helixi935 – 939Combined sources5
Helixi942 – 945Combined sources4
Helixi946 – 950Combined sources5
Helixi956 – 968Combined sources13
Helixi978 – 981Combined sources4
Helixi987 – 993Combined sources7
Turni994 – 996Combined sources3
Helixi1015 – 1018Combined sources4
Helixi1023 – 1025Combined sources3
Helixi1026 – 1036Combined sources11
Helixi1041 – 1050Combined sources10
Helixi1055 – 1062Combined sources8
Helixi1066 – 1070Combined sources5
Helixi1075 – 1086Combined sources12
Helixi1091 – 1102Combined sources12
Beta strandi1103 – 1105Combined sources3
Helixi1117 – 1125Combined sources9
Turni1129 – 1131Combined sources3
Beta strandi1137 – 1145Combined sources9
Beta strandi1150 – 1153Combined sources4
Beta strandi1159 – 1162Combined sources4
Beta strandi1166 – 1169Combined sources4
Turni1177 – 1179Combined sources3
Beta strandi1185 – 1194Combined sources10
Helixi1195 – 1197Combined sources3
Beta strandi1199 – 1203Combined sources5
Helixi1206 – 1209Combined sources4
Turni1222 – 1224Combined sources3
Helixi1227 – 1246Combined sources20
Turni1247 – 1249Combined sources3
Beta strandi1253 – 1255Combined sources3
Helixi1264 – 1271Combined sources8
Beta strandi1279 – 1283Combined sources5
Turni1285 – 1287Combined sources3
Beta strandi1288 – 1298Combined sources11
Beta strandi1301 – 1311Combined sources11
Beta strandi1313 – 1317Combined sources5
Beta strandi1320 – 1324Combined sources5
Beta strandi1327 – 1331Combined sources5
Helixi1332 – 1338Combined sources7
Helixi1340 – 1351Combined sources12
Helixi1361 – 1372Combined sources12
Beta strandi1380 – 1385Combined sources6
Beta strandi1387 – 1389Combined sources3
Beta strandi1392 – 1400Combined sources9
Beta strandi1406 – 1412Combined sources7
Beta strandi1414 – 1419Combined sources6
Beta strandi1422 – 1426Combined sources5
Helixi1427 – 1437Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L3TNMR-A1250-1440[»]
3OAKX-ray2.15C/D239-263[»]
3PSFX-ray2.59A235-1259[»]
3PSIX-ray3.30A239-1451[»]
3PSJX-ray2.70A1247-1451[»]
3PSKX-ray2.10A/B/C/D1247-1451[»]
ProteinModelPortaliP23615.
SMRiP23615.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23615.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1257 – 1354SH2PROSITE-ProRule annotationAdd BLAST98

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi8 – 12Nuclear localization signalSequence analysis5
Motifi77 – 85Nuclear localization signalSequence analysis9
Motifi120 – 125Nuclear localization signalSequence analysis6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 484Asp/Glu-rich (acidic)Add BLAST484

Sequence similaritiesi

Belongs to the SPT6 family.Curated
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

GeneTreeiENSGT00510000047446.
HOGENOMiHOG000193710.
InParanoidiP23615.
KOiK11292.
OMAiWNDEREL.
OrthoDBiEOG092C04AI.

Family and domain databases

Gene3Di1.10.150.310. 1 hit.
1.10.3500.10. 2 hits.
3.30.420.140. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR010994. RuvA_2-like.
IPR000980. SH2.
IPR028083. Spt6_acidic_N_dom.
IPR027999. Spt6_dom.
IPR032706. Spt6_HHH.
IPR028088. Spt6_HTH_DNA-bd_dom.
IPR028231. Spt6_YqgF.
IPR023323. Tex-like_dom.
IPR023097. Tex_RuvX-like_dom.
IPR017072. TF_Spt6.
IPR006641. YqgF/RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10145:SF6. PTHR10145:SF6. 2 hits.
PfamiPF14878. DLD. 1 hit.
PF14635. HHH_7. 1 hit.
PF14641. HTH_44. 1 hit.
PF14633. SH2_2. 1 hit.
PF14632. SPT6_acidic. 1 hit.
PF14639. YqgF. 1 hit.
[Graphical view]
PIRSFiPIRSF036947. Spt6. 1 hit.
SMARTiSM00252. SH2. 1 hit.
SM00732. YqgFc. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23615-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEETGDSKLV PRDEEEIVND NDETKAPSEE EEGEDVFDSS EEDEDIDEDE
60 70 80 90 100
DEARKVQEGF IVNDDDENED PGTSISKKRR KHKRREREED DRLSEDDLDL
110 120 130 140 150
LMENAGVERT KASSSSGKFK RLKRVGDEGN AAESESDNVA ASRQDSTSKL
160 170 180 190 200
EDFFSEDEEE EESGLRNGRN NEYGRDEEDH ENRNRTADKG GILDELDDFI
210 220 230 240 250
EDDEFSDEDD ETRQRRIQEK KLLREQSIKQ PTQITGLSSD KIDEMYDIFG
260 270 280 290 300
DGHDYDWALE IENEELENGN DNNEAEEEEI DEETGAIKST KKKISLQDIY
310 320 330 340 350
DLEDLKKNLM TEGDMKIRKT DIPERYQELR AGITDYGNMS SEDQELERNW
360 370 380 390 400
IAEKISVDKN FDANYDLTEF KEAIGNAIKF ITKENLEVPF IYAYRRNYIS
410 420 430 440 450
SREKDGFLLT EDDLWDIVSL DIEFHSLVNK KDYVQRFYAE LHIDDPIVTE
460 470 480 490 500
YFKNQNTASI AELNSLQDIY DYLEFKYANE INEMFINHTG KTGKKHLKNS
510 520 530 540 550
SYEKFKASPL YQAVSDIGIS AEDVGENISS QHQIHPPVDH PSSKPVEVIE
560 570 580 590 600
SILNANSGDL QVFTSNTKLA IDTVQKYYSL ELSKNTKIRE KVRSDFSKYY
610 620 630 640 650
LADVVLTAKG KKEIQKGSLY EDIKYAINRT PMHFRRDPDV FLKMVEAESL
660 670 680 690 700
NLLSVKLHMS SQAQYIEHLF QIALETTNTS DIAIEWNNFR KLAFNQAMDK
710 720 730 740 750
IFQDISQEVK DNLTKNCQKL VAKTVRHKFM TKLDQAPFIP NVRDPKIPKI
760 770 780 790 800
LSLTCGQGRF GADAIIAVYV NRKGDFIRDY KIVDNPFDKT NPEKFEDTLD
810 820 830 840 850
NIIQSCQPNA IGINGPNPKT QKFYKRLQEV LHKKQIVDSR GHTIPIIYVE
860 870 880 890 900
DEVAIRYQNS ERAAQEFPNK PPLVKYCIAL ARYMHSPLLE YANLTSEEVR
910 920 930 940 950
SLSIHPHQNL LSSEQLSWAL ETAFVDIVNL VSVEVNKATD NNYYASALKY
960 970 980 990 1000
ISGFGKRKAI DFLQSLQRLN EPLLARQQLI THNILHKTIF MNSAGFLYIS
1010 1020 1030 1040 1050
WNEKRQKYED LEHDQLDSTR IHPEDYHLAT KVAADALEYD PDTIAEKEEQ
1060 1070 1080 1090 1100
GTMSEFIELL REDPDRRAKL ESLNLESYAE ELEKNTGLRK LNNLNTIVLE
1110 1120 1130 1140 1150
LLDGFEELRN DFHPLQGDEI FQSLTGESEK TFFKGSIIPV RVERFWHNDI
1160 1170 1180 1190 1200
ICTTNSEVEC VVNAQRHAGA QLRRPANEIY EIGKTYPAKV IYIDYANITA
1210 1220 1230 1240 1250
EVSLLDHDVK QQYVPISYSK DPSIWDLKQE LEDAEEERKL MMAEARAKRT
1260 1270 1280 1290 1300
HRVINHPYYF PFNGRQAEDY LRSKERGEFV IRQSSRGDDH LVITWKLDKD
1310 1320 1330 1340 1350
LFQHIDIQEL EKENPLALGK VLIVDNQKYN DLDQIIVEYL QNKVRLLNEM
1360 1370 1380 1390 1400
TSSEKFKSGT KKDVVKFIED YSRVNPNKSV YYFSLNHDNP GWFYLMFKIN
1410 1420 1430 1440 1450
ANSKLYTWNV KLTNTGYFLV NYNYPSVIQL CNGFKTLLKS NSSKNRMNNY

R
Length:1,451
Mass (Da):168,291
Last modified:November 1, 1991 - v1
Checksum:i0BE9922A59BD0483
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34391 Genomic DNA. Translation: AAA35086.1.
Z72899 Genomic DNA. Translation: CAA97124.1.
Z72902 Genomic DNA. Translation: CAA97127.1.
BK006941 Genomic DNA. Translation: DAA08208.1.
PIRiA36468.
RefSeqiNP_011631.1. NM_001181245.1.

Genome annotation databases

EnsemblFungiiYGR116W; YGR116W; YGR116W.
GeneIDi853011.
KEGGisce:YGR116W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34391 Genomic DNA. Translation: AAA35086.1.
Z72899 Genomic DNA. Translation: CAA97124.1.
Z72902 Genomic DNA. Translation: CAA97127.1.
BK006941 Genomic DNA. Translation: DAA08208.1.
PIRiA36468.
RefSeqiNP_011631.1. NM_001181245.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L3TNMR-A1250-1440[»]
3OAKX-ray2.15C/D239-263[»]
3PSFX-ray2.59A235-1259[»]
3PSIX-ray3.30A239-1451[»]
3PSJX-ray2.70A1247-1451[»]
3PSKX-ray2.10A/B/C/D1247-1451[»]
ProteinModelPortaliP23615.
SMRiP23615.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33361. 70 interactors.
DIPiDIP-2630N.
IntActiP23615. 21 interactors.
MINTiMINT-427185.

PTM databases

iPTMnetiP23615.

Proteomic databases

MaxQBiP23615.
PRIDEiP23615.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR116W; YGR116W; YGR116W.
GeneIDi853011.
KEGGisce:YGR116W.

Organism-specific databases

EuPathDBiFungiDB:YGR116W.
SGDiS000003348. SPT6.

Phylogenomic databases

GeneTreeiENSGT00510000047446.
HOGENOMiHOG000193710.
InParanoidiP23615.
KOiK11292.
OMAiWNDEREL.
OrthoDBiEOG092C04AI.

Enzyme and pathway databases

BioCyciYEAST:G3O-30823-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP23615.
PROiP23615.

Family and domain databases

Gene3Di1.10.150.310. 1 hit.
1.10.3500.10. 2 hits.
3.30.420.140. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR010994. RuvA_2-like.
IPR000980. SH2.
IPR028083. Spt6_acidic_N_dom.
IPR027999. Spt6_dom.
IPR032706. Spt6_HHH.
IPR028088. Spt6_HTH_DNA-bd_dom.
IPR028231. Spt6_YqgF.
IPR023323. Tex-like_dom.
IPR023097. Tex_RuvX-like_dom.
IPR017072. TF_Spt6.
IPR006641. YqgF/RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10145:SF6. PTHR10145:SF6. 2 hits.
PfamiPF14878. DLD. 1 hit.
PF14635. HHH_7. 1 hit.
PF14641. HTH_44. 1 hit.
PF14633. SH2_2. 1 hit.
PF14632. SPT6_acidic. 1 hit.
PF14639. YqgF. 1 hit.
[Graphical view]
PIRSFiPIRSF036947. Spt6. 1 hit.
SMARTiSM00252. SH2. 1 hit.
SM00732. YqgFc. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPT6_YEAST
AccessioniPrimary (citable) accession number: P23615
Secondary accession number(s): D6VUP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 30, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8890 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.